LOCUS       sequence-47638-e         822 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Vander Haar E, Lee SI, Bandhakavi S, Griffin TJ, Kim DH
  TITLE     Insulin signalling to mTOR mediated by the Akt/PKB substrate PRAS40.
  JOURNAL   Nat Cell Biol. 2007 Mar . 9(3):316-23.
  PUBMED    17277771
REFERENCE   2  (bases 1 to 822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Cell 
            Biol. 2007 Mar . 9(3):316-23."
COMMENT     Sequence Label: pRK5-HA-PRAS40 T246A
FEATURES             Location/Qualifiers
     source          1..822
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      82..91
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             94..120
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 cactcccagg tccaactgca cctcggttct atcgattgaa ttcccgaacc gacagtcggt
       61 ctcttcacca aggccattcg cgccaccatg ggctacccct atgatgtgcc tgactacgca
      121 gatctcaatg gtggtggtgg tgggtcgacc atggcgtcgg gtcgccctga ggagctgtgg
      181 gaggccgtgg tgggggccgc tgagcgcttc cgggcccgga ctggcacgga gctggtgctg
      241 ctgaccgcgg ccccgccgcc accaccccgc ccgggcccct gtgcctatcc tgcccatggt
      301 cgaggagccc tggcggaggc agcgcgccgt tgcctccacg acatcgcact ggcccacagg
      361 gctgccactg ctgctcggcc tcctgcgccc ccaccagcac cacagccacc cagtcccaca
      421 cccagcccac cccggcctac cctggccaga gaggacaacg aggaggacga ggatgagccc
      481 acagagacag agacctccgg ggagcagctg ggcattagtg ataatggagg gctctttgtg
      541 atggatgagg acgccaccct ccaggacctt ccccccttct gtgagtcaga ccccgagagt
      601 acagatgatg gcagcctgag cgaggagacc cccgccggcc cccccacctg ctcagtgccc
      661 ccagcctcag ccctacccac acagcagtac gccaagtccc tgcctgtgtc tgtgcccgtc
      721 tggggcttca aggagaagag gacagaggcg cggtcatcag atgaggagaa tgggccgccc
      781 tcttcgcccg acctggaccg catcgcggcg agcatgcgcg cg
//
