LOCUS       sequence-476401-        7455 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express peroxisomal Venus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7455)
  TITLE     Dueber Lab plasmids 2025
REFERENCE   2  (bases 1 to 7455)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pJJB112
FEATURES             Location/Qualifiers
     source          1..7455
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             707..934
                     /codon_start=1
                     /product="Saccharomyces cerevisiae ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     CDS             1061..1771
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKL
                     ICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEGGNILGHKLEYNYNSHNVYITADKQKNGIKAN
                     FKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     promoter        2486..2893
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             2894..3988
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     CDS             complement(5019..5834)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(5019..5834)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     5742..5761
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     5826..5845
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      6059..6647
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6548..6567
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aagaggatgt ccaatatttt ttttaaggaa taaggatact tcaagactag attcccccct
       61 gcattcccat cagaaccgta aaccttggcg ctttccttgg gaagtattca agaagtgcct
      121 tgtccggttt ctgtggctca caaaccagcg cgcccgatat ggctttcttt tcacttatga
      181 atgtaccagt acgggacaat tagaacgctc ctgtaacaat ctctttgcaa atgtggggtt
      241 acattctaac catgtcacac tgctgacgaa attcaaagta aaaaaaaatg ggaccacgtc
      301 ttgagaacga tagattttct ttattttaca ttgaacagtc gttgtctcag cgcgctttat
      361 gttttcattc atacttcata ttataaaata acaaaagaag aatttcatat tcacgcccaa
      421 gaaatcaggc tgctttccaa atgcaattga cacttcatta gccatcacac aaaactcttt
      481 cttgctggag cttcttttaa aaaagacctc agtacaccaa acacgttacc cgacctcgtt
      541 attttacgac aactatgata aaattctgaa gaaaaaataa aaaaattttc atacttcttg
      601 cttttattta aaccattgaa tgatttcttt tgaacaaaac tacctgtttc accaaaggaa
      661 atagaaagaa aaaatcaatt agaagaaaac aaaaaacaaa agatctatgc agattttcgt
      721 taaaacttta actggtaaaa caattacttt agaagttgag tcatctgata ctatcgataa
      781 tgttaaatct aagatccagg ataaggaagg tattcctcca gatcaacaaa gattgatttt
      841 cgcaggtaag cagttggagg acggtaggac tttgtctgat tataacatac agaaagaatc
      901 tactttgcac ttagtcttaa gattaagggg aggtagacat ggttctggtg cctggttgtt
      961 acctgtatca ttggttaaaa gaaagaccac attggcacca aatactcaaa ctgcctctcc
     1021 aagagccttg gcagactctt taatgcagag atcaggttct tctaaaggtg aagaattatt
     1081 cactggtgtt gtcccaattt tggttgaatt agatggtgat gttaatggtc acaaattttc
     1141 tgtctccggt gaaggtgaag gtgatgctac ttacggtaaa ttgaccttaa aattgatttg
     1201 tactactggt aaattgccag ttccatggcc aaccttagtc actactttag gttatggttt
     1261 gcaatgtttt gctagatacc cagatcatat gaaacaacat gactttttca agtctgccat
     1321 gccagaaggt tatgttcaag aaagaactat ttttttcaaa gatgacggta actacaagac
     1381 cagagctgaa gtcaagtttg aaggtgatac cttagttaat agaatcgaat taaaaggtat
     1441 tgattttaaa gaaggtggta acattttagg tcacaaattg gaatacaact ataactctca
     1501 caatgtttac atcactgctg acaaacaaaa gaatggtatc aaagctaact tcaaaattag
     1561 acacaacatt gaagatggtg gtgttcaatt agctgaccat tatcaacaaa atactccaat
     1621 tggtgatggt ccagtcttgt taccagacaa ccattactta tcctatcaat ctgccttatc
     1681 caaagatcca aacgaaaaga gagatcacat ggtcttgtta gaatttgtta ctgctgctgg
     1741 tattacccat ggtatggatg aattgtacaa agggtccttg ggaagaggta gaagatccaa
     1801 attgtaagga tcctaactcg agagtgcttt taactaagaa ttattagtct tttctgctta
     1861 ttttttcatc atagtttaga acactttata ttaacgaata gtttatgaat ctatttaggt
     1921 ttaaaaattg atacagtttt ataagttact ttttcaaaga ctcgtgctgt ctattgcata
     1981 atgcactgga aggggaaaaa aaaggtgcac acgcgtggct ttttcttgaa tttgcagttt
     2041 gaaaaatgct ggaaatctgc tcgtcagtgg tgctcacact gacgaatcat gtacagatca
     2101 taccgatgac tgcctggcga ctcacaacta agcaagacag ccggaaccag cgccggcgaa
     2161 caccactgca tatatggcat atcacaacag tccacgtctc aagcagttac agagatgtta
     2221 cgaaccacta gtgcactgca gtacatcgag gagaacttct agtatatcta catacctaat
     2281 attattgcct tattaaaaat ggaatcccaa caattacatc aaaatccaca ttctcttcaa
     2341 aatcaattgt cctgtacttc cttgttcatg tgtgttcaaa aacgttatat ttataggata
     2401 attatactct atttctcaac aagtaattgg ttgtttggcc gagcggtcta aggcgcctga
     2461 ttcaagaaat atcttgaccg cagttaactg tgggaatact caggtatcgt aagatgcaag
     2521 agttcgaatc tcttagcaac cattattttt ttcctcaaca taacgagaac acacaggggc
     2581 gctatcgcac agaatcaaat tcgatgactg gaaatttttt gttaatttca gaggtcgcct
     2641 gacgcatata cctttttcaa ctgaaaaatt gggagaaaaa ggaaaggtga gagcgccgga
     2701 accggctttt catatagaat agagaagcgt tcatgactaa atgcttgcat cacaatactt
     2761 gaagttgaca atattattta aggacctatt gttttttcca ataggtggtt agcaatcgtc
     2821 ttactttcta acttttctta ccttttacat ttcagcaata tatatatata tatttcaagg
     2881 atataccatt ctaatgtctg cccctaagaa gatcgtcgtt ttgccaggtg accacgttgg
     2941 tcaagaaatc acagccgaag ccattaaggt tcttaaagct atttctgatg ttcgttccaa
     3001 tgtcaagttc gatttcgaaa atcatttaat tggtggtgct gctatcgatg ctacaggtgt
     3061 tccacttcca gatgaggcgc tggaagcctc caagaaggct gatgccgttt tgttaggtgc
     3121 tgtgggtggt cctaaatggg gaaccggtag tgttagacct gaacaaggtt tactaaaaat
     3181 ccgtaaagaa cttcaattgt acgccaactt aagaccatgt aactttgcat ccgactctct
     3241 tttagactta tctccaatca agccacaatt tgctaaaggt actgacttcg ttgttgtcag
     3301 agaattagtg ggaggtattt actttggtaa gagaaaggaa gacgatggtg atggtgtcgc
     3361 ttgggatagt gaacaataca ccgttccaga agtgcaaaga atcacaagaa tggccgcttt
     3421 catggcccta caacatgagc caccattgcc tatttggtcc ttggataaag ctaatgtttt
     3481 ggcctcttca agattatgga gaaaaactgt ggaggaaacc atcaagaacg aatttcctac
     3541 attgaaggtt caacatcaat tgattgattc tgccgccatg atcctagtta agaacccaac
     3601 ccacctaaat ggtattataa tcaccagcaa catgtttggt gatatcatct ccgatgaagc
     3661 ctccgttatc ccaggttcct tgggtttgtt gccatctgcg tccttggcct ctttgccaga
     3721 caagaacacc gcatttggtt tgtacgaacc atgccacggt tctgctccag atttgccaaa
     3781 gaataaggtc aaccctatcg ccactatctt gtctgctgca atgatgttga aattgtcatt
     3841 gaacttgcct gaagaaggta aggccattga agatgcagtt aaaaaggttt tggatgcagg
     3901 tatcagaact ggtgatttag gtggttccaa cagtaccacc gaagtcggtg atgctgtcgc
     3961 cgaagaagtt aagaaaatcc ttgcttaaaa agattctctt tttttatgat atttgtacat
     4021 aaactttata aatgaaattc ataatagaaa cgacacgaaa ttacaaaatg gaatatgttc
     4081 atagggtaga cgaaactata tacgcaatct acatacattt atcaagaagg agaaaaagga
     4141 ggatgtaaag gaatacaggt aagcaaattg atactaatgg ctcaacgtga taaggaaaaa
     4201 gaattgcact ttaacattaa tattgacaag gaggagggca ccacacaaaa agttaggtgt
     4261 aacagaaaat catgaaacta tgattcctaa tttatatatt ggaggatttt ctctaaaaaa
     4321 aaaaaaatac aacaaataaa aaacactcaa tgacctgacc atttgatgga gtttaagtca
     4381 ataccttctt gaaccatttc ccataatggt gaaagttccc tcaagaattt tactctgtca
     4441 gaaacggcct taacgacgta gtcgagagta ctcgtatcgc atgtcggtgc gacacgaaat
     4501 tacaaaatgg aatatgttca tagggtagac gaaactatat acgcaatcta catacattta
     4561 tcaagaagga gaaaaaggag gatgtaaagg aatacaggta agcaaattga tactaatggc
     4621 tcaacgtgat aaggaaaaag aattgcactt taacattaat attgacaagg aggagggcac
     4681 cacacaaaaa gttaggtgta acagaaaatc atgaaactat gattcctaat ttatatattg
     4741 gaggattttc tctaaaaaaa aaaaaataca acaaataaaa aacactcaat gacctgacca
     4801 tttgatggag tttaagtcaa taccttcttg aaccatttcc cataatggtg aaagttccct
     4861 caagaatttt actctgtcag aaacggcctt aacgacgtag tcgacctcct cttcagtact
     4921 aaatctacca ataccaaatc tgatggaaga atgggctaat gcatcatcct tacccagcgc
     4981 cgagcggccg cgtgttacaa ccaattaacc aattctgatt agaaaaactc atcgagcatc
     5041 aaatgaaact gcaatttatt catatcagga ttatcaatac catatttttg aaaaagccgt
     5101 ttctgtaatg aaggagaaaa ctcaccgagg cagttccata ggatggcaag atcctggtat
     5161 cggtctgcga ttccgactcg tccaacatca atacaaccta ttaatttccc ctcgtcaaaa
     5221 ataaggttat caagtgagaa atcaccatga gtgacgactg aatccggtga gaatggcaaa
     5281 agcttatgca tttctttcca gacttgttca acaggccagc cattacgctc gtcatcaaaa
     5341 tcactcgcat caaccaaacc gttattcatt cgtgattgcg cctgagcgag gcgaaatacg
     5401 cgatcgctgt taaaaggaca attacaaaca ggaatcgaat gcaaccggcg caggaacact
     5461 gccagcgcat caacaatatt ttcacctgaa tcaggatatt cttctaatac ctggaatgct
     5521 gttttcccgg ggatcgcagt ggtgagtaac catgcatcat caggagtacg gataaaatgc
     5581 ttgatggtcg gaagaggcat aaattccgtc agccagttta gtctgaccat ctcatctgta
     5641 acatcattgg caacgctacc tttgccatgt ttcagaaaca actctggcgc atcgggcttc
     5701 ccatacaatc gatagattgt cgcacctgat tgcccgacat tatcgcgagc ccatttatac
     5761 ccatataaat cagcatccat gttggaattt aatcgcggcc tggagcaaga cgtttcccgt
     5821 tgaatatggc tcataacacc ccttgtatta ctgtttatgt aagcagacag ttttattgtt
     5881 catgatgata tatttttatc ttgtgcaatg taacatcaga gattttgaga cacaacgtgg
     5941 ctttgttgaa taaatcgaac ttttgctgag ttgaaggatc agtcatgacc aaaatccctt
     6001 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     6061 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     6121 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     6181 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     6241 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     6301 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     6361 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     6421 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     6481 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     6541 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     6601 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     6661 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     6721 tatcccctga ttctgtggat aaccgtgcgg ccgccaatac ccagaagaac agtaaaataa
     6781 agcaaggtac gtgaaattaa tatttttaaa tggttctaac cgatgccgaa gaactgcgca
     6841 gtccggttat aacgtctgac atgtcctttt ttgatttgga atccaaccac tcaagtgact
     6901 ctgttcattt actttgcgaa aaatataccc acaaattgcc catcgaaagt gaatcgcaaa
     6961 ccaccttcag actggcaccg acaaagcaaa gattatacag acagagtact ttatacgtac
     7021 cgttaagtct caagcaaagg gttttcttat ttactgaacg ggtaaagagt atctgggccg
     7081 gcttgccaag atgcaaaccg aataagtatt tcaaagttgc atttgcctta gccgtcctga
     7141 caccattggc tatttggata ttttatattg actttcgtgt acattgatca catcgactgt
     7201 tctattggca aatgaaccac gggcattgac tatttttcag gttactacta tatattatca
     7261 tcacgggcaa ggattgtacc ctgaattcgc atctagactg atgagacgtg gtagagccac
     7321 aaacagccgg tacaagcaac gatctccagg accatctgaa tcatgcgcgg atgacacgaa
     7381 ctcacgacgg cgatcacaga cattaaccca cagtacagac actgcgacaa cgtggcaatt
     7441 cgtcgcaata caacg
//
