LOCUS       sequence-476402-        9369 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-terminal fusion of NanoLuc luciferase to dSaCas9, mammalian 
            expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9369)
  TITLE     A Split Luciferase Biosensing Platform for Detection and Imaging of 
            Chromatin Loops in Individual Live Cells
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9369)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: dSaCas9-NLuc
FEATURES             Location/Qualifiers
     source          1..9369
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    1040..1161
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1051..1070)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1105..1124
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(1337..2131)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1448..1467)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     2058..2077
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(2198..2527)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2212..2347
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(2266..2285)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     2512..2532
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(2541..2969)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2651..2672)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2863..2882
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    3171..3219
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     3244..3263
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(3327..3374)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             complement(7163..7228)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             complement(7247..7267)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(7364..7388)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(7385..7588)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(7414..7434)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        7589..7968
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     8140..8159
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        8234..8338
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8339..8407,8408..9199)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8557..8576)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagctct agctagaggt
     1021 cgacggtata cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg
     1081 cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt
     1141 ataagctgca ataaacaagt tggggtgggc gaagaactcc agcatgagat ccccgcgctg
     1201 gaggatcatc cagccggcgt cccggaaaac gattccgaag cccaaccttt catagaaggc
     1261 ggcggtggaa tcgaaatctc gtgatggcag gttgggcgtc gcttggtcgg tcatttcgcg
     1321 aaccccagag tcccgctcag aagaactcgt caagaaggcg atagaaggcg atgcgctgcg
     1381 aatcgggagc ggcgataccg taaagcacga ggaagcggtc agcccattcg ccgccaagct
     1441 cttcagcaat atcacgggta gccaacgcta tgtcctgata gcggtccgcc acacccagcc
     1501 ggccacagtc gatgaatcca gaaaagcggc cattttccac catgatattc ggcaagcagg
     1561 catcgccatg ggtcacgacg agatcctcgc cgtcgggcat gcgcgccttg agcctggcga
     1621 acagttcggc tggcgcgagc ccctgatgct cttcgtccag atcatcctga tcgacaagac
     1681 cggcttccat ccgagtacgt gctcgctcga tgcgatgttt cgcttggtgg tcgaatgggc
     1741 aggtagccgg atcaagcgta tgcagccgcc gcattgcatc agccatgatg gatactttct
     1801 cggcaggagc aaggtgagat gacaggagat cctgccccgg cacttcgccc aatagcagcc
     1861 agtcccttcc cgcttcagtg acaacgtcga gcacagctgc gcaaggaacg cccgtcgtgg
     1921 ccagccacga tagccgcgct gcctcgtcct gcagttcatt cagggcaccg gacaggtcgg
     1981 tcttgacaaa aagaaccggg cgcccctgcg ctgacagccg gaacacggcg gcatcagagc
     2041 agccgattgt ctgttgtgcc cagtcatagc cgaatagcct ctccacccaa gcggccggag
     2101 aacctgcgtg caatccatct tgttcaatca tgcgaaacga tcctcatcct gtctcttgat
     2161 cagatccgaa aatggatata caagctcccg ggagcttttt gcaaaagcct aggcctccaa
     2221 aaaagcctcc tcactacttc tggaatagct cagaggcaga ggcggcctcg gcctctgcat
     2281 aaataaaaaa aattagtcag ccatggggcg gagaatgggc ggaactgggc ggagttaggg
     2341 gcgggatggg cggagttagg ggcgggacta tggttgctga ctaattgaga tgcatgcttt
     2401 gcatacttct gcctgctggg gagcctgggg actttccaca cctggttgct gactaattga
     2461 gatgcatgct ttgcatactt ctgcctgctg gggagcctgg ggactttcca caccctaact
     2521 gacacacatt ccacagaatt aattcgcgtt aaatttttgt taaatcagct cattttttaa
     2581 ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt
     2641 gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa
     2701 agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag
     2761 ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt
     2821 tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg
     2881 agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc
     2941 cgcgcttaat gcgccgctac agggcgcgtg gggatacccc ctagagcccc agctgcgcag
     3001 atctgctatg gcagggcctg ccgccccgac gttggctgcg agccctgggc cttcacccga
     3061 acttgggggg tggggtgggg aaaaggaaga aacgcgggcg tattggcccc aatggggtct
     3121 cggtggggta tcgacagagt gccagccctg ggaccgaacc ccgcgtttat gaacaaacga
     3181 cccaacaccc gtgcgtttta ttctgtcttt ttattgccgt catagcgcgg gttccttccg
     3241 gtattgtctc cttccgtgtt tcagttagcc tcccccgttt aaactcatta ctaaccggta
     3301 gggatcgaac cctttcatgc ggccgccttt ttcttttttg cctgaccggc ctttttcgtg
     3361 gccgccggcc ttttagatcc acctccggag cctccaccga gaatctcctc gaacagccgg
     3421 tagccggtca cactgttgat ggttactcgg aacagcatgg agccgtcggg ggtgatcagg
     3481 cgctcgtcga taattttgtt gccgttccac agggtccctg ttacagtgat ctttttgccg
     3541 tcgaacacgg cgatgccttc atacggccgt ccgaaatagt tcagcatgtt cggcgtaacc
     3601 ccgtcgatta ccagtgtgcc atagggcagg atcaccttaa agtgatgatc atccacaggg
     3661 tacaccacct taaacacctc ttcgatctgg gccatttggt cggcgctcag accttcatac
     3721 gggatgatga catggatgtc gatcttcagg gcattttcac cgctccggac aatcctttgg
     3781 atcggagtta cggacacggc gagattctgc agcaaactgg acacacctcc ctgttcaagg
     3841 acttggtcca ggttgtaggc ggctgtctgt tcccagtccc caacgaaatc ttcgagtgtg
     3901 aagaccatgc tccctccgct ccccccggat cctcctgaac ctccactacc accgctagcg
     3961 ccctttttga tgatctgagg gtgcttctta gatttcactt catacaggtt gcccagaatg
     4021 tctgtgctgt acttcttaat gctctgggtc ttggaggcga ttgtcttaat gatcctgggg
     4081 ggcctcttgt cgttcatgtt ttccaggtac tcgcggtagg tgatgtcgat catgttcact
     4141 tcgatccggt tcagcaggtc gttgttcacg ccgatcactc tatacagctc gccgttgatc
     4201 ttgatcagat cgttgttgta gaaggaggcg ataaactcgg cctggttgct gatcttcttc
     4261 agcttcttag cttcctcata gcacttgcta ttcacttcgt agtagttttc ttttttgatc
     4321 acatccagat tcttcacggt cacgaacttg tacacgccat tgtccaggta cacgtcgaat
     4381 ctgtagggct tcagggacag cttcacgacc ttgtttctgc tgttggggta gtcgtcggtg
     4441 atgtccagat gggcgttcag tttgttgccg taatacttaa tcttcttgat cacggggccg
     4501 ttgtcctttt tggagtactt ggtcaggtag ttcccggttt cctcgtagta cttgtacagg
     4561 ggattcttct cgtcgccgta ctgttccata atcagcttca gtttctggta ggtctggggg
     4621 tcgtggtggt acatcagcag cttttcgggg ctcttgttga tcagcttttt cagcttgtca
     4681 ttgtccttgt cgtacaggcc gttcagattg ttcacgatca gggtgttgcc cttgtcgtcc
     4741 ttccgggtgg agtacagggt gtcgttaatc agctctctat taggcttctt gtccacccgg
     4801 tggctgtact tgtagtcctt gaagtcctta atgtgcttga tctggtgggg ggtgatgaag
     4861 atctctttgt actcctgctc ggtttcgatc tcgggcatgc tctcggcctg cttttcctcg
     4921 aacatctggt tttccatcac ttttttggcc ttgtccagtt tcttccactc tttgaagatg
     4981 aaatcggcgt tggcaatgat cagggcgtcc tcggcgtggt gcttgtaccc cttgttccgc
     5041 tctttcttaa acttccactt ccgccgcaga aagctggtga agccgccatt gatggacttc
     5101 actttcacgt ccaggttgtt cactctgaag tagctccgca gcaggttcat caggcctctg
     5161 gtggcgtatc tggtatccac caggttccgg ttgatgaagt ctttctgcac ggagaacctg
     5221 ttgatgtccc gttcttccag cagatactct ttcttggtct tgctgattct gcccttgccc
     5281 ttggccagat tcaggatgtg cttcttgaag gtttcgtagc tgatcttgct gtcgctgctg
     5341 ctcaggtact ggaatggggt ccggttgccc ttcttgctgg cttcttcctg cttcacgagc
     5401 accttgttgt tgaagctgtt gtcgaaggac acgcttctgg ggatgatgtg gtccacctca
     5461 tagttgaagg ggttgttcag cagatcttcc agagggatgg cttccaggct gtacaggcac
     5521 ttgccttcct gcatgtcgtg cagcttgatc ttctcgatca ggtacttggc gttctctttg
     5581 ccggtggtcc ggatgatttc ctcgatccgc tcgttggtct gccggttccg cttctgcatc
     5641 tcgttgatca ttttctgggc gtccttggag ttcttctcgc gggccagctc gataatgatg
     5701 tcgttgggca ggccgtactt cttgatgatg gcgttgatca ctttgatgct ctggatgaag
     5761 cttctcttca cgacggggct caggatgaag tcgtccacca gggtggtggg gatctctttc
     5821 tgctgggaca ggtccacctt cttgggcacc agcttcagcc ggttgaagat agcgatctgg
     5881 ttgtcgttgg tgtgccacag ctcgtccagg atcaggttga tggccttcag gctcaggttg
     5941 tgggtgccgg tatagccctt cagattagag atctgctcga tctcttcctg ggtcagctcg
     6001 gagttcagat tggtcagttc ttcctggatg tcctcgctgc tctggtagat ggtcaggatc
     6061 ttggcaatct gatccagcag ctcggcgttc tcaataatct ctttccgggc ggtaatgtcc
     6121 ttgatgtcgt ggtacacctt caggttggtg aactcgggct tgccggtgct ggtcactctg
     6181 tagcccttaa tatcctcttc gttcacgagg atttctttgg cgatctgctt cagggtgggc
     6241 ttcttcttct gcttgaacac gttctcgatg atctggaact tctcgtaata ttccagcttc
     6301 tcgttctcgt ccctggtgat cacgagattg ttcaggtcgt tcagggcgtt gtacaggtcg
     6361 gcgttgtagg cgtacttcac gctccgcagt tcctcgggga agtaggtgca gtggcccatc
     6421 agcatctcgt accattcttt gatgtccttc cagccgaagg ggctgccctc gccaggtccc
     6481 tcatagtagg tccgccgggt ttccagcagg tcgatgtagg tgtcgatgaa gctctggtcc
     6541 agctggtggt aggccttctg caccttcagc agctgtttgg cttctttcac gtagtcgctg
     6601 gtcttgaatc tgttgatgct gccccgcact tcgccgtctt tcttcagccg ttccagctgc
     6661 agttcggcca cgtatttctc ttccagggcc ttgctgttcc ggctgatctg ctctttggtg
     6721 gacagctcgt tgccggtgtc ctcttccacc tcgttcacgt tgtgcacgcc tcttctcttg
     6781 gccaggtgca gcagggcggc agagaactct tcctcgctca gcttctggct caggcccttc
     6841 actctggcct cgtaggggtt gatgccgctc agctcgctgt ggtcggtcag caggttgtag
     6901 tcgaacagca gcttcttcac tctctggatt ctatgccgcc tccgccgctt cagccttctg
     6961 gcgcctctct tgctccgcct gccctcgttg ttttccacgt tggcctcttt gaacagccgc
     7021 acgccggcat cgatcacgtc ccgtgtctcg tagtcgatga tgccgtagcc cacgctggtg
     7081 atgccgatgg ccaggcccag gatgtagttc cgctttggcc ggccgctcag gctggtaccg
     7141 gatccaccgc cgacccgcca cccttatcgt catcgtcttt gtaatcaata tcatgatcct
     7201 tgtagtctcc gtcgtggtcc ttatagtcgc ttcctccgga gcctcccacc ttcctcttct
     7261 tcttgggcat ggtggcaagg gttcgatcct ctagagtccg gaggctggat cggtcccggt
     7321 gtcttctatg gaggtcaaaa cagcgtggat ggcgtctcca ggcgatctga cggttcacta
     7381 aacgagctct gcttatatag acctcccacc gtacacgcct accgcccatt tgcgtcaatg
     7441 gggcggagtt gttacgacat tttggaaagt cccgttgatt ttggtgccaa aacaaactcc
     7501 cattgacgtc aatggggtgg agacttggaa atccccgtga gtcaaaccgc tatccacgcc
     7561 cattgatgta ctgccaaaac cgcatcacca tggtaatagc gatgactaat acgtagatgt
     7621 actgccaagt aggaaagtcc cataaggtca tgtactgggc ataatgccag gcgggccatt
     7681 taccgtcatt gacgtcaata gggggcgtac ttggcatatg atacacttga tgtactgcca
     7741 agtgggcagt ttaccgtaaa tactccaccc attgacgtca atggaaagtc cctattggcg
     7801 ttactatggg aacatacgtc attattgacg tcaatgggcg ggggtcgttg ggcggtcagc
     7861 caggcgggcc atttaccgta agttatgtaa cgcggaactc catatatggg ctatgaacta
     7921 atgaccccgt aattgattac tattaataac tagtcaataa tcaatgtcaa cgcgtatatc
     7981 tggcccgtac atcgcgaagc agcgcaaaac gcctaaccct aagcagattc ttcatgcaat
     8041 tgtcggtcaa gccttgcctt gttgtagctt aaattttgct cgcgcactac tcagcgacct
     8101 ccaacacaca agcagggagc agatactggc ttaactatgc ggcatcagag cagattgtac
     8161 tgagagtgca ccatagggga tcgggagatc tcccgatccg tcgacgtcag gtggcacttt
     8221 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     8281 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     8341 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     8401 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     8461 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     8521 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     8581 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     8641 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     8701 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     8761 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     8821 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     8881 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     8941 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     9001 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     9061 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     9121 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     9181 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     9241 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     9301 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     9361 aggatcttc
//
