LOCUS       sequence-47646-e         957 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 957)
  AUTHORS   Knobel PA, Kotov IN, Felley-Bosco E, Stahel RA, Marti TM
  TITLE     Inhibition of REV3 expression induces persistent DNA damage and 
            growth arrest in cancer cells.
  JOURNAL   Neoplasia. 2011 Oct;13(10):961-70.
  PUBMED    22028621
REFERENCE   2  (bases 1 to 957)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Neoplasia."; date: "2011-10"; volume: "13(10)"; pages: "961-70"
COMMENT     Sequence Label: pSUPERIOR.puro-shREV3-4
FEATURES             Location/Qualifiers
     source          1..957
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     41..57
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     42..58
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    124..142
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(188..207)
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     promoter        353..852
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(388..411)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     785..804
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     complement(877..896)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 ccctcactaa agggaacaaa agctggtacc gggccccccc tcgaggtcga cggtatcgat
       61 aagcttaaaa acaaagatgc tgctacatta tctcttgaat aatgtagcag catctttggg
      121 ggatctctat cactgatagg gaacttataa gattcccaaa tccaaagaca tttcacgttt
      181 atggtgattt cccagaacac atagcgacat gcaaatattg cagggcgcca ctcccctgtc
      241 cctcacagcc atcttcctgc cagggcgcac gcgcgctggg tgttcccgcc tagtgacact
      301 gggcccgcga ttccttggag cgggttgatg acgtcagcgt tcgaattcta ccgggtaggg
      361 gaggcgcttt tcccaaggca gtctggagca tgcgctttag cagccccgct gggcacttgg
      421 cgctacacaa gtggcctctg gcctcgcaca cattccacat ccaccggtag gcgccaaccg
      481 gctccgttct ttggtggccc cttcgcgcca ccttctactc ctcccctagt caggaagttc
      541 ccccccgccc cgcagctcgc gtcgtgcagg acgtgacaaa tggaagtagc acgtctcact
      601 agtctcgtgc agatggacag caccgctgag caatggaagc gggtaggcct ttggggcagc
      661 ggccaatagc agctttgctc cttcgctttc tgggctcaga ggctgggaag gggtgggtcc
      721 gggggcgggc tcaggggcgg gctcaggggc ggggcgggcg cccgaaggtc ctccggaggc
      781 ccggcattct gcacgcttca aaagcgcacg tctgccgcgc tgttctcctc ttcctcatct
      841 ccgggccttt cgacctgcag cccaagctag cttaccatga ccgagtacaa gcccacggtg
      901 cgcctcnnnc ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcg
//
