LOCUS       sequence-476556-        3095 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  express tyrosinase protein and melanin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3095)
  AUTHORS   Collins M, Lau MB, Ma W, Shen A, Wang B, Cai S, La Russa M, Jewett 
            MC, Qi LS
  TITLE     A frugal CRISPR kit for equitable and accessible education in gene 
            editing and synthetic biology.
  JOURNAL   Nat Commun. 2024 Aug 3;15(1):6563. doi: 10.1038/s41467-024-50767-2.
  PUBMED    39095367
REFERENCE   2  (bases 1 to 3095)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2024-08-3"; volume: "15(1):6563. doi"; pages: " 
            10.1038/s41467-024-50767-2"
COMMENT     Sequence Label: pSLQ13960 tyrosinase(Priestia megaterium)
FEATURES             Location/Qualifiers
     source          1..3095
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(747..774)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(790..861)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     promoter        complement(1811..1845)
                     /label=J23119(SpeI) promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119) modified to end with an SpeI site"
     promoter        1919..2023
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2024..2092,2093..2884)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2242..2261)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     terminator      2914..3008
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgccctagg tataaacgca gaaaggccca cccgaaggtg agccagtgtg
      781 actctagtag agagcgttca ccgacaaaca acagataaaa cgaaaggccc agtctttcga
      841 ctgagccttt cgttttattt gatgcctgga gatccttact cgagttagct agatcgcttg
      901 gactttctca gctcgatgtc gtacacgtag cccagctttc tgtggttcat cacgtcctct
      961 ggggtggtgt tccaggggta catagggtct ctgaaatttt gtccgaaggg gccgtttttc
     1021 ataggctggt aattctgatc ccggtgcacg atctgccaca cgctccagat tctatccacg
     1081 ttggcgtggt gcaggaagaa aacggggtcg ttaggagcgg tgggcaccac gcccatctgg
     1141 cctccgaccc atctgtgcac ccggttgtgc agctgagggc cgttgataaa gccttccagc
     1201 tggttcctaa agctgttttg gctggtcata tcccaaggtg gggtatcgta ctgggtgatt
     1261 ttcagggcgt tcagcacgtc gtctcttgtg ggcagtgttg gggcttcctt agtggcgccg
     1321 aagttccgct tcagtccgcc gctaggattg ccctgctcgt cgatggttgt ccatctgcca
     1381 gcggcgaaag ggccggtgtc cacgatgaag tccttcttag ggttgccatt tcctcccatg
     1441 aagccggcgg accagatagg agactggctg gggtcctgga gctgggcgtc tgtctcccat
     1501 tcccagtaag gcagggtcac ctcggggttc acagactgca gatctctctc gaaccgcagc
     1561 aggtattccc gatgccaagg cagaaaggcg ctgctcatgt gagcggcgtt ccggtcgctg
     1621 ccaggagggg tgtggaactt gccggcagcg ccgtgccagg cgatgtatct gtcatagatg
     1681 cccttttcct tcaggatcag gacggctcta atgaaatctc ttttctcggt atctgtcagg
     1741 tgcagcacgt tcttccgcac tctgtacttg ttgctcatag atcctttctc cctttagatc
     1801 ttttgaattc actagtatta tacctaggac tgagctagct gtcaagcgca acgcaattaa
     1861 tgtaagttag ctcactcatt aggcaccgac gtcaggtggc acttttcggg gaaatgtgcg
     1921 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     1981 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     2041 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     2101 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     2161 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     2221 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     2281 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     2341 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     2401 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     2461 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     2521 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     2581 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     2641 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     2701 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     2761 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     2821 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     2881 gtaactgtca gaccaagttt acgagctcgc ttggactcct gttgatagat ccagtaatga
     2941 cctcagaact ccatctggat ttgttcagaa cgctcggttg ccgccgggcg ttttttattg
     3001 gtgagaatcc aagcactagt catgaccaaa atcccttaac gtgagttttc gttccactga
     3061 gcgtcagacc ccgtagaaaa gatcaaagga tcttc
//
