LOCUS       sequence-476558-        2916 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  express fwYellow chromoprotein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2916)
  AUTHORS   Collins M, Lau MB, Ma W, Shen A, Wang B, Cai S, La Russa M, Jewett 
            MC, Qi LS
  TITLE     A frugal CRISPR kit for equitable and accessible education in gene 
            editing and synthetic biology.
  JOURNAL   Nat Commun. 2024 Aug 3;15(1):6563. doi: 10.1038/s41467-024-50767-2.
  PUBMED    39095367
REFERENCE   2  (bases 1 to 2916)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2024-08-3"; volume: "15(1):6563. doi"; pages: " 
            10.1038/s41467-024-50767-2"
COMMENT     Sequence Label: pSLQ3534 fwYellow
FEATURES             Location/Qualifiers
     source          1..2916
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(747..774)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(790..861)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     RBS             1605..1616
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     promoter        complement(1632..1666)
                     /label=J23119(SpeI) promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119) modified to end with an SpeI site"
     promoter        1740..1844
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1845..1913,1914..2705)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2063..2082)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     terminator      2735..2829
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgccctagg tataaacgca gaaaggccca cccgaaggtg agccagtgtg
      781 actctagtag agagcgttca ccgacaaaca acagataaaa cgaaaggccc agtctttcga
      841 ctgagccttt cgttttattt gatgcctgga gatccttact cgagttatta acggtacgtc
      901 tccaggtcaa ctgccttgat cacctcaaca atgttcaaat ggtcacggtg atcggtgacg
      961 tctttagaca gggtaacatg atacgtaatg tggtggtact ccggcacgtg caccggacca
     1021 ccaccaatcg gggtattcat ttgggtgtga tcggcaacaa tgaaatcctc tttggagccg
     1081 gtgatctcat gcatgatttt gaacgcgctc ttcaggccat gattcgcttg gtcaccccaa
     1141 atgtacaaac aatgaggggt aaagttaaac tccagatttt tgcccagcac gtggccgtcc
     1201 ttcttgaaac cctggccgtt cagttttaca cggttgtaca cgctgccatt ttcaaaggtg
     1261 acttccgcac gggtcttgaa cacgccatca ccctcaaagg tgatggtgcg ctcttggacg
     1321 taaccttccg gcatgcagct tttgaagaaa tccgcaatgt ggcgcggata cttggcaaag
     1381 cactgagcac catacgtcag cgtggtgacc agcgtgctcc acggaaccgg aacgtcgccc
     1441 gtggtacaaa tgaactgagc atccacttta ccgacgctcg cgtcaccggt accttcaccc
     1501 ttgatgataa acttcatacc ttcaacgtca ccttccagct cagtgatata tgggatttct
     1561 ttctcgaaca gttttgcgcc ttcagtcagt gccgtcatag atcctttctc ctctttagat
     1621 cttttgaatt cactagtatt atacctagga ctgagctagc tgtcaagcgc aacgcaatta
     1681 atgtaagtta gctcactcat taggcaccga cgtcaggtgg cacttttcgg ggaaatgtgc
     1741 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     1801 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     1861 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     1921 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     1981 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     2041 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     2101 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     2161 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     2221 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     2281 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     2341 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     2401 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     2461 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     2521 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     2581 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     2641 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     2701 ggtaactgtc agaccaagtt tacgagctcg cttggactcc tgttgataga tccagtaatg
     2761 acctcagaac tccatctgga tttgttcaga acgctcggtt gccgccgggc gttttttatt
     2821 ggtgagaatc caagcactag tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     2881 agcgtcagac cccgtagaaa agatcaaagg atcttc
//
