LOCUS       sequence-476559-        2889 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  express eforRed chromoprotein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2889)
  AUTHORS   Collins M, Lau MB, Ma W, Shen A, Wang B, Cai S, La Russa M, Jewett 
            MC, Qi LS
  TITLE     A frugal CRISPR kit for equitable and accessible education in gene 
            editing and synthetic biology.
  JOURNAL   Nat Commun. 2024 Aug 3;15(1):6563. doi: 10.1038/s41467-024-50767-2.
  PUBMED    39095367
REFERENCE   2  (bases 1 to 2889)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2024-08-3"; volume: "15(1):6563. doi"; pages: " 
            10.1038/s41467-024-50767-2"
COMMENT     Sequence Label: pSLQ3533 eforRed
FEATURES             Location/Qualifiers
     source          1..2889
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(747..774)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(790..861)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     RBS             1578..1589
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     promoter        complement(1605..1639)
                     /label=J23119(SpeI) promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119) modified to end with an SpeI site"
     promoter        1713..1817
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1818..1886,1887..2678)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2036..2055)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     terminator      2708..2802
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgccctagg tataaacgca gaaaggccca cccgaaggtg agccagtgtg
      781 actctagtag agagcgttca ccgacaaaca acagataaaa cgaaaggccc agtctttcga
      841 ctgagccttt cgttttattt gatgcctgga gatccttact cgagttatta tgggagagcc
      901 ttcggcagcg gtgagaaatg gccttcggct gcctcctgct ggtcccaagt tgtgaaatct
      961 ttatcgtgtt tggtcagttc caggcgatgt tcaacgaaat gaaattctgg cagcttgaca
     1021 ggttttttgg ctttatacgt cgttttgaaa tcaacacgat gatgaccgcc tcctttcagt
     1081 ttaaaaacca ttgcagtgtc actttttaac atcccgtcac gcacgtacag tgtttccacg
     1141 ctcggctccc atccagcaat ggttttttgc attacgggcc catccggagg caggttcaca
     1201 ccatgaaatt taacatcata gatgaaacag ttctcttgca agtttatgct ggagtgatta
     1261 ctggcggtgc atacgccacc atcttcaaaa atcatcgacc gctcccaaga cataccttcg
     1321 ggaaaagact gtttgtggta gtctgggata tccgctggat acttattaaa cggtttgctt
     1381 ccatacatgt ggctaggtgt aagaatatga acggaaaacg gcagaggcgc gcctttggtg
     1441 actgtcattt tcatgtgctg taacccttcg tacggcttgc cttcaccttt accctcgatc
     1501 gtaaaatcat ggccattgac agtgccctca aggtgcaact tggtcttcat tacctgctta
     1561 atcactgaca tagatccttt ctcctcttta gatcttttga attcactagt attataccta
     1621 ggactgagct agctgtcaag cgcaacgcaa ttaatgtaag ttagctcact cattaggcac
     1681 cgacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     1741 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     1801 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     1861 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     1921 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     1981 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     2041 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     2101 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     2161 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     2221 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     2281 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     2341 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     2401 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     2461 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     2521 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     2581 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     2641 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttacgagc
     2701 tcgcttggac tcctgttgat agatccagta atgacctcag aactccatct ggatttgttc
     2761 agaacgctcg gttgccgccg ggcgtttttt attggtgaga atccaagcac tagtcatgac
     2821 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     2881 aggatcttc
//
