LOCUS       sequence-476564-       10530 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This plasmid encodes the full-length hamster (Mesocricetus auratus) 
            TMEM106B protein fused with a 3xHA tag, along with an N-terminal 
            blasticidin selection marker..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10530)
  AUTHORS   Baggen J, Jacquemyn M, Persoons L, Vanstreels E, Pye VE, Wrobel AG, 
            Calvaresi V, Martin SR, Roustan C, Cronin NB, Reading E, Thibaut HJ,
            Vercruysse T, Maes P, De Smet F, Yee A, Nivitchanyong T, Roell M, 
            Franco-Hernandez N, Rhinn H, Mamchak AA, Ah Young-Chapon M, Brown E,
            Cherepanov P, Daelemans D
  TITLE     TMEM106B is a receptor mediating ACE2-independent SARS-CoV-2 cell 
            entry.
  JOURNAL   Cell. 2023 Aug 3;186(16):3427-3442.e22. doi: 
            10.1016/j.cell.2023.06.005. Epub 2023 Jul 7.
  PUBMED    37421949
REFERENCE   2  (bases 1 to 10530)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2023.06.005"; journalName: "Cell"; date: "2023-08-3-
            3"; volume: "186"; issue: "16"; pages: "3427-3442.e22"
COMMENT     Sequence Label: pLCKO-CMV-Blasticidin-P2A-3xHA-TMEM106B_Hamster
FEATURES             Location/Qualifiers
     source          1..10530
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        138..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..721
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     672..692
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     718..742
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      840..849
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             846..1241
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             1251..1307
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             1308..1388
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     misc_feature    2238..2826
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2291..2311)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2709..2720)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2898..3131
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3209..3330
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3246..3265)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3300..3319
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3370..3505
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3525..3544)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3526..3544)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3554..3571)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3554..3570)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3563..3585)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3712..4167
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3799..3818)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4009..4030
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4193..4297
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4298..4366,4367..5158)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4516..4535)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5329..5917
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5818..5837
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6071..6088
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6205..6226
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6241..6246,6247..6264,6265..6271)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6279..6295
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6284..6306
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6303..6319
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6303..6319
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6338..6358
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6340..6358
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6386..6612
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6613..6793
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6840..6965
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7458..7691
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7876..7920
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8098..8346
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     8098..8118
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     8269..8288
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        10238..10313
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    10361..10478
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tcgtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgggc ctcggcggcc aagcttggca atccggtact gttggtaaag
      841 ccaccatggc caagcctttg tctcaagaag aatccaccct cattgaaaga gcaacggcta
      901 caatcaacag catccccatc tctgaagact acagcgtcgc cagcgcagct ctctctagcg
      961 acggccgcat cttcactggt gtcaatgtat atcattttac tgggggacct tgtgcagaac
     1021 tcgtggtgct gggcactgct gctgctgcgg cagctggcaa cctgacttgt atcgtcgcga
     1081 tcggaaatga gaacaggggc atcttgagcc cctgcggacg gtgccgacag gtgcttctcg
     1141 atctgcatcc tgggatcaaa gccatagtga aggacagtga tggacagccg acggcagttg
     1201 ggattcgtga attgctgccc tctggttatg tgtgggaggg cggcagcgga gcaaccaatt
     1261 ttagtctcct caaacaggcc ggcgatgtcg aagaaaatcc gggcccatac ccatacgatg
     1321 ttccagatta cgcttatccc tacgacgtgc ctgattatgc atacccatat gatgtccccg
     1381 actatgccgg atccatggga aagtctcttt ctcatttacc tttgcattca aataaagaag
     1441 atggttatga tggggtcaca tcgacagaca acatgaggac tggactcgtt aacaacgaag
     1501 tgcataatga agatggaagg aatggagatg tctctcagtt tccgtatgtg gaatttaccg
     1561 gaagagatag tgtcacttgt cccacttgcc aaggaacagg aagaattcct agggggcaag
     1621 aaaaccagct ggtggcattg atcccctata gtgatcaacg gttacggcca agaagaacaa
     1681 agctgtatgt gatggcatct gtgtctgtct gcctgctcct gtctggattg gctgtgtttt
     1741 tccttttccc tcgatctatt gatgtgaagt acattggcgt aaaatcagcc tatgtcagtt
     1801 atgattttca aaagcgaacc atatatttaa atatcacgaa cacactaaat ataacaaaca
     1861 ataactatta ttcggttgaa gttgaaaaca tcactgctca agttcagttt tcaaaaacag
     1921 ttattggaaa ggcacgctta aacaacataa ctaacattgg cccactggat atgaagcaga
     1981 ttgattatac ggtacccaca gttattgctg aagaaatgag ttacatgtat gacttctgta
     2041 cactgatttc catcaaagtg cacaacatag tacttatgat gcaagtaact gtaacaacaa
     2101 catactttgg acactctgag caaatatctc aggagaggta ccagtacgtc gattgtggaa
     2161 ggaacacgac ttaccagttg gcccagtctg agtatctaaa tgtgcttcag ccacaacagt
     2221 gaacgcgtta agtcgacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta
     2281 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
     2341 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
     2401 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
     2461 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
     2521 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
     2581 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt
     2641 cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct
     2701 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
     2761 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
     2821 ccccgcgtcg actttaagac caatgactta caaggcagct gtagatctta gccacttttt
     2881 aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag atctgctttt
     2941 tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     3001 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     3061 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     3121 aatctctagc agtacgtata gtagttcatg tcatcttatt attcagtatt tataacttgc
     3181 aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa
     3241 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     3301 tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta
     3361 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     3421 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
     3481 tagtgaggag gcttttttgg aggcctaggg acgtacccaa ttcgccctat agtgagtcgt
     3541 attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta
     3601 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg
     3661 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg gacgcgccct
     3721 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     3781 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     3841 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     3901 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
     3961 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     4021 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
     4081 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     4141 ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg tgcgcggaac
     4201 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     4261 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     4321 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     4381 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     4441 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     4501 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
     4561 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     4621 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     4681 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     4741 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     4801 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     4861 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     4921 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     4981 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     5041 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     5101 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     5161 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     5221 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     5281 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     5341 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     5401 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     5461 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     5521 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     5581 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     5641 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     5701 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     5761 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     5821 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     5881 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     5941 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     6001 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     6061 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc
     6121 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
     6181 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc
     6241 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
     6301 cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc actaaaggga
     6361 acaaaagctg gagctgcaag cttaatgtag tcttatgcaa tactcttgta gtcttgcaac
     6421 atggtaacga tgagttagca acatgcctta caaggagaga aaaagcaccg tgcatgccga
     6481 ttggtggaag taaggtggta cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca
     6541 tggattggac gaaccactga attgccgcat tgcagagata ttgtatttaa gtgcctagct
     6601 cgatacataa acgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     6661 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     6721 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     6781 aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc
     6841 tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac
     6901 tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga
     6961 gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag
     7021 ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat
     7081 tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc
     7141 tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa
     7201 ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga
     7261 tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccgct gatcttcaga
     7321 cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta
     7381 aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa
     7441 aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact
     7501 atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg
     7561 cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca
     7621 gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat
     7681 caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct
     7741 tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg
     7801 gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg
     7861 caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg
     7921 tggaattggt ttaacataac aaattggctg tggtatataa aattattcat aatgatagta
     7981 ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg
     8041 cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg acccagagag
     8101 ggcctatttc ccatgattcc ttcatatttg catatacgat acaaggctgt tagagagata
     8161 attagaatta atttgactgt aaacacaaag atattagtac aaaatacgtg acgtagaaag
     8221 taataatttc ttgggtagtt tgcagtttta aaattatgtt ttaaaatgga ctatcatatg
     8281 cttaccgtaa cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga
     8341 ggtaccggta ggcaggtgtg ttgtaaatga gcacacaaaa tacacatgct aaaatattat
     8401 attctatgac ctttataaaa tcaaccaaaa tcttcttttt aataacttta gtatcaataa
     8461 ttagaatttt tatgttcctt tttgcaaact tttaataaaa atgagcaaaa taaaaaaacg
     8521 ctagttttag taactcgcgt tgttttcttc acctttaata atagctactc caccacttgt
     8581 tcctaagcgg tcagctcctg cttcaatcat tttttgagca tcttcaaatg ttctaactcc
     8641 accagctgct ttaactaaag cattgtcttt aacaactgac ttcattagtt taacatcttc
     8701 aaatgttgca cctgattttg aaaatcctgt tgatgtttta acaaattcta atccagcttc
     8761 aacagctatt tcacaagctt tcatgatttc ttcttttgtt aataaacaat tttccataat
     8821 acatttaaca acatgtgatc cagctgcttt ttttacagct ttcatgtctt ctaaaactaa
     8881 ttcataattt ttgtctttta atgcaccaat atttaatacc atatcaattt ctgttgcacc
     8941 atctttaatt gcttcagaaa cttcgaatgc ttttgtagct gttgtgcatg cacctagagg
     9001 aaaacctaca acatttgtta ttcctacatt tgtgcctttt aataattctt tacaatagct
     9061 tgttcaatat gaattaacac aaactgttgc aaaatcaaat tcaattgctt catcacataa
     9121 ttgtttaatt tcagctttcg tagcatcttg ttttaataat gtgtgatcta tatatttgtt
     9181 tagtttcatt ttttctccta tatattcatt tttaatttta attctttaat aatttcgtct
     9241 actttaactt tagcgttttg aacagattca ccaacaccta taaaataaat ttttagttta
     9301 ggttcagttc cacttgggcg aacagcaaat catgacttat cttctaaata aaattttagt
     9361 aagtcttgtc ctggcatatt atacattcca tcgatgtagt cttcaacatt aacaacttta
     9421 agtccagcaa tttgagttaa gggtgttgct ctcaatgatt tcattaatgg ttcaattttt
     9481 aatttctttt cttctggttt aaaattcaag tttaaagtga aagtgtaata tgcacccatt
     9541 tctttaaata aatcttctaa atagtctact aatgttttat tttgtttttt ataaaatcaa
     9601 gcagcctctg ctattaatat agaagcttgt attccatctt tatctctagc tgagtcatca
     9661 attacatatc cataactttc ttcataagca aaaacaaaat ttaatccgtt atcttcttct
     9721 ttagcaattt ctctacccat tcatttaaat ccagttaaag tttttacaat attaactcca
     9781 tatttttcat gagcgattct atcacccaaa tcacttgtta caaaacttga atatagagcc
     9841 ggattttttg gaatgctatt taagcgtttt agatttgata attttcaatc aattaaaatt
     9901 ggtcctgttt gatttccatc taatcttaca aaatgaccat catgttttat tgccattcca
     9961 aatctgtcag catctgggtc attcataata ataatatctg catcatgttt aataccatat
    10021 tcaagcggta tttttcatgc aggatcaaat tctggatttg gatttacaac atttttaaat
    10081 gtttcatctt caaatgcatg ctcttcaacc tcaataacgt tatatcctga ttcacgtaat
    10141 atttttgggg taaatttagt tcctgttcca ttaactgcgc taaaaataat ttttaaatct
    10201 tttttagctt cttgctcttt tttgtagacc tgcagaggtt ttagagctag aaatagcaag
    10261 ttaaaataag gctagtccgt tatcaacttg aaaaagtggc accgagtcgg tgcttttttg
    10321 aattctagat cttgagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
    10381 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
    10441 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
    10501 gagatccact ttggcgccgg ctcgaggggg
//
