LOCUS       sequence-47657-e         860 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Miyamoto T, Derose R, Suarez A, Ueno T, Chen M, Sun TP, Wolfgang MJ,
            Mukherjee C, Meyers DJ, Inoue T
  TITLE     Rapid and orthogonal logic gating with a gibberellin-induced 
            dimerization system.
  JOURNAL   Nat Chem Biol. 2012 Mar 25;8(5):465-70. doi: 10.1038/nchembio.922.
  PUBMED    22446836
REFERENCE   2  (bases 1 to 860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nchembio"; 
            journalName: "Nat Chem Biol"; date: "2012-03-25- 25"; volume: "8"; 
            issue: "5"; pages: "465-70"
COMMENT     Sequence Label: CFP-GAI(1-172)
FEATURES             Location/Qualifiers
     source          1..860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(680..697)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(764..785)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta
       61 aagcaagtaa aacctctaca aatgtggtat ggctgattat gatcagttat ctagatccgg
      121 tggatcccgg gcccgcggta ccgtcgactg cagaattcga agcttgagct ccaccagcac
      181 taccaccagc actcgcgtga acgagacgca caccgttctc ctgcgagtca accaggacaa
      241 catgccgagt tgactcagcc gtcgctgtag tggtttccac gacgccgttt gagcatttca
      301 accgcttgtt tgtagtatac gtatctcctc cgccgccttg gttagacgaa gaagccgaat
      361 cgatagcgaa ctgattgaga atcgcgtcac cgggaatagc tttaagatcg tactcggcgt
      421 tagacgacgg aggattaagg tcggtgagca tagaatcaag ccacgtgtaa agctccgccg
      481 gattatagtg aacagtctca gtagcgagtt gagaaagatc gtcttcttga acattagaca
      541 tcataacttc aagctgctcg agtttctgag caacatcagc catttcggat gacctaacct
      601 tgtaaccaag aacagctaga agctcatcca tgccgttacc gtcgtcttct tcattcatca
      661 tcatagtctt cttatcttga tgatgatgat gatgatgatc tctcttcatt cctcctgcgc
      721 tcttgtacag ctcgtccatg ccgagagtga tcccggcggc ggtcacgaac tccagcagga
      781 ccatgtgatc gcgcttctcg ttggggtctt tgctcagggc ggactgggtg ctcaggtagt
      841 ggttgtcggg cagcagcacg
//
