LOCUS       sequence-476570-        9645 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes full length human ACE2 protein, along with blasticidin 
            selection marker..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9645)
  AUTHORS   Baggen J, Jacquemyn M, Persoons L, Vanstreels E, Pye VE, Wrobel AG, 
            Calvaresi V, Martin SR, Roustan C, Cronin NB, Reading E, Thibaut HJ,
            Vercruysse T, Maes P, De Smet F, Yee A, Nivitchanyong T, Roell M, 
            Franco-Hernandez N, Rhinn H, Mamchak AA, Ah Young-Chapon M, Brown E,
            Cherepanov P, Daelemans D
  TITLE     TMEM106B is a receptor mediating ACE2-independent SARS-CoV-2 cell 
            entry.
  JOURNAL   Cell. 2023 Aug 3;186(16):3427-3442.e22. doi: 
            10.1016/j.cell.2023.06.005. Epub 2023 Jul 7.
  PUBMED    37421949
REFERENCE   2  (bases 1 to 9645)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2023.06.005"; journalName: "Cell"; date: "2023-08-3-
            3"; volume: "186"; issue: "16"; pages: "3427-3442.e22"
COMMENT     Sequence Label: pLCKO-CMV-ACE2-P2A-Blasticidin
FEATURES             Location/Qualifiers
     source          1..9645
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3143..3199
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3200..3595
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="AKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTGV
                     NVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGIK
                     AIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    3611..4199
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3664..3684)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4082..4093)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4271..4504
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4582..4703
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4619..4638)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4673..4692
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4743..4878
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(4899..4917)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4927..4944)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4927..4943)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4936..4958)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5085..5540
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5172..5191)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5382..5403
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5566..5670
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5671..5739,5740..6531)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5889..5908)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6702..7290
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7191..7210
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7444..7461
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7578..7599
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7614..7619,7620..7637,7638..7644)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7652..7668
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7657..7679
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7676..7692
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7676..7692
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7711..7731
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7713..7731
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7759..7985
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7986..8166
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8213..8338
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8831..9064
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9249..9293
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9480..9597
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctg
      661 gctagcgttt aaacgggccc tctagaccat gtcaagctct tcctggctcc ttctcagcct
      721 tgttgctgta actgctgctc agtccaccat tgaggaacag gccaagacat ttttggacaa
      781 gtttaaccac gaagccgaag acctgttcta tcaaagttca cttgcttctt ggaattataa
      841 caccaatatt actgaagaga atgtccaaaa catgaataat gctggggaca aatggtctgc
      901 ctttttaaag gaacagtcca cacttgccca aatgtatcca ctacaagaaa ttcagaatct
      961 cacagtcaag cttcagctgc aggctcttca gcaaaatggg tcttcagtgc tctcagaaga
     1021 caagagcaaa cggttgaaca caattctaaa tacaatgagc accatctaca gtactggaaa
     1081 agtttgtaac ccagataatc cacaagaatg cttattactt gaaccaggtt tgaatgaaat
     1141 aatggcaaac agtttagact acaatgagag gctctgggct tgggaaagct ggagatctga
     1201 ggtcggcaag cagctgaggc cattatatga agagtatgtg gtcttgaaaa atgagatggc
     1261 aagagcaaat cattatgagg actatgggga ttattggaga ggagactatg aagtaaatgg
     1321 ggtagatggc tatgactaca gccgcggcca gttgattgaa gatgtggaac atacctttga
     1381 agagattaaa ccattatatg aacatcttca tgcctatgtg agggcaaagt tgatgaatgc
     1441 ctatccttcc tatatcagtc caattggatg cctccctgct catttgcttg gtgatatgtg
     1501 gggtagattt tggacaaatc tgtactcttt gacagttccc tttggacaga aaccaaacat
     1561 agatgttact gatgcaatgg tggaccaggc ctgggatgca cagagaatat tcaaggaggc
     1621 cgagaagttc tttgtatctg ttggtcttcc taatatgact caaggattct gggaaaattc
     1681 catgctaacg gacccaggaa atgttcagaa agcagtctgc catcccacag cttgggacct
     1741 ggggaagggc gacttcagga tccttatgtg cacaaaggtg acaatggacg acttcctgac
     1801 agctcatcat gagatggggc atatccagta tgatatggca tatgctgcac aaccttttct
     1861 gctaagaaat ggagctaatg aaggattcca tgaagctgtt ggggaaatca tgtcactttc
     1921 tgcagccaca cctaagcatt taaaatccat tggtcttctg tcacccgatt ttcaagaaga
     1981 caatgaaaca gaaataaact tcctgctcaa acaagcactc acgattgttg ggactctgcc
     2041 atttacttac atgttagaga agtggaggtg gatggtcttt aaaggggaaa ttcccaaaga
     2101 ccagtggatg aaaaagtggt gggagatgaa gcgagagata gttggagtgg tggaacctgt
     2161 gccccatgat gaaacatact gtgaccccgc atctctgttc catgtttcta atgattactc
     2221 attcattcga tattacacaa ggacccttta ccaattccag tttcaagaag cactttgtca
     2281 agcagctaaa catgaaggcc ctctgcacaa atgtgacatc tcaaactcta cagaagctgg
     2341 acagaaactg ttcaatatgc tgaggcttgg aaaatcagaa ccctggaccc tagcattgga
     2401 aaatgttgta ggagcaaaga acatgaatgt aaggccactg ctcaactact ttgagccctt
     2461 atttacctgg ctgaaagacc agaacaagaa ttcttttgtg ggatggagta ccgactggag
     2521 tccatatgca gaccaaagca tcaaagtgag gataagccta aaatcagctc ttggagataa
     2581 agcatatgaa tggaacgaca atgaaatgta cctgttccga tcatctgttg catatgctat
     2641 gaggcagtac tttttaaaag taaaaaatca gatgattctt tttggggagg aggatgtgcg
     2701 agtggctaat ttgaaaccaa gaatctcctt taatttcttt gtcactgcac ctaaaaatgt
     2761 gtctgatatc attcctagaa ctgaagttga aaaggccatc aggatgtccc ggagccgtat
     2821 caatgatgct ttccgtctga atgacaacag cctagagttt ctggggatac agccaacact
     2881 tggacctcct aaccagcccc ctgtttccat atggctgatt gtttttggag ttgtgatggg
     2941 agtgatagtg gttggcattg tcatcctgat cttcactggg atcagagatc ggaagaagaa
     3001 aaataaagca agaagtggag aaaatcctta tgcctccatc gatattagca aaggagaaaa
     3061 taatccagga ttccaaaaca ctgatgatgt tcagacctcc tttggtaccg agacctccca
     3121 ggtggcgccc gctggcagcg gagcaaccaa ttttagtctc ctcaaacagg ccggcgatgt
     3181 cgaagaaaat ccgggcccag ccaagccttt gtctcaagaa gaatccaccc tcattgaaag
     3241 agcaacggct acaatcaaca gcatccccat ctctgaagac tacagcgtcg ccagcgcagc
     3301 tctctctagc gacggccgca tcttcactgg tgtcaatgta tatcatttta ctgggggacc
     3361 ttgtgcagaa ctcgtggtgc tgggcactgc tgctgctgcg gcagctggca acctgacttg
     3421 tatcgtcgcg atcggaaatg agaacagggg catcttgagc ccctgcggac ggtgccgaca
     3481 ggtgcttctc gatctgcatc ctgggatcaa agccatagtg aaggacagtg atggacagcc
     3541 gacggcagtt gggattcgtg aattgctgcc ctctggttat gtgtgggagg gctgaacgcg
     3601 ttaagtcgac aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     3661 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     3721 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
     3781 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
     3841 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
     3901 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
     3961 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc
     4021 ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
     4081 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
     4141 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcg
     4201 tcgactttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa
     4261 aaggggggac tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt
     4321 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     4381 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     4441 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     4501 agcagtacgt atagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa
     4561 tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc
     4621 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     4681 tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc
     4741 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     4801 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
     4861 gaggcttttt tggaggccta gggacgtacc caattcgccc tatagtgagt cgtattacgc
     4921 gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact
     4981 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac
     5041 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg
     5101 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     5161 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     5221 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     5281 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
     5341 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     5401 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
     5461 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
     5521 aatattaacg cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     5581 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     5641 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     5701 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     5761 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     5821 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     5881 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     5941 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     6001 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     6061 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     6121 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     6181 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     6241 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     6301 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     6361 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     6421 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     6481 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     6541 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     6601 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     6661 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     6721 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     6781 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     6841 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     6901 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     6961 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     7021 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     7081 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     7141 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     7201 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     7261 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     7321 ctggtctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     7381 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     7441 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     7501 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
     7561 agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac
     7621 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga
     7681 aacagctatg accatgatta cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag
     7741 ctggagctgc aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa
     7801 cgatgagtta gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg
     7861 aagtaaggtg gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg
     7921 gacgaaccac tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca
     7981 taaacgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     8041 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     8101 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     8161 ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg
     8221 acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag
     8281 tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag
     8341 tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa
     8401 gaaaaaatat aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt
     8461 taatcctggc ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc
     8521 atcccttcag acaggatcag aagaacttag atcattatat aatacagtag caaccctcta
     8581 ttgtgtgcat caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga
     8641 agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag
     8701 gaggagatat gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg
     8761 aaccattagg agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag
     8821 cagtgggaat aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg
     8881 cagcgtcaat gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc
     8941 agaacaattt gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg
     9001 gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc
     9061 tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg
     9121 ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg
     9181 acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc
     9241 agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt
     9301 ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct
     9361 tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat
     9421 attcaccatt atcgtttcag acccacctcc caaccccgag gggacccaga gagggcctat
     9481 tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata
     9541 gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttcgg
     9601 gtttattaca gggacagcag agatccactt tggcgccggc tcgag
//
