LOCUS       sequence-476571-       10527 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This plasmid encodes the full-length human TMEM106B protein fused 
            with a 3xHA tag, along with an N-terminal blasticidin selection 
            marker..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10527)
  AUTHORS   Baggen J, Jacquemyn M, Persoons L, Vanstreels E, Pye VE, Wrobel AG, 
            Calvaresi V, Martin SR, Roustan C, Cronin NB, Reading E, Thibaut HJ,
            Vercruysse T, Maes P, De Smet F, Yee A, Nivitchanyong T, Roell M, 
            Franco-Hernandez N, Rhinn H, Mamchak AA, Ah Young-Chapon M, Brown E,
            Cherepanov P, Daelemans D
  TITLE     TMEM106B is a receptor mediating ACE2-independent SARS-CoV-2 cell 
            entry.
  JOURNAL   Cell. 2023 Aug 3;186(16):3427-3442.e22. doi: 
            10.1016/j.cell.2023.06.005. Epub 2023 Jul 7.
  PUBMED    37421949
REFERENCE   2  (bases 1 to 10527)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2023.06.005"; journalName: "Cell"; date: "2023-08-3-
            3"; volume: "186"; issue: "16"; pages: "3427-3442.e22"
COMMENT     Sequence Label: pLCKO-CMV-Blasticidin-P2A-3xHA-TMEM106B_Human
FEATURES             Location/Qualifiers
     source          1..10527
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        138..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..721
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     672..692
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     718..742
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      840..849
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             846..1241
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             1251..1307
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             1308..1388
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     misc_feature    2235..2823
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2288..2308)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2706..2717)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2895..3128
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3206..3327
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3243..3262)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3297..3316
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3367..3502
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3522..3541)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3523..3541)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3551..3568)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3551..3567)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3560..3582)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3709..4164
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3796..3815)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4006..4027
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4190..4294
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4295..4363,4364..5155)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4513..4532)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5326..5914
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5815..5834
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6068..6085
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6202..6223
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6238..6243,6244..6261,6262..6268)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6276..6292
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6281..6303
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6300..6316
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6300..6316
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6335..6355
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6337..6355
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6383..6609
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6610..6790
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6837..6962
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7455..7688
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7873..7917
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8095..8343
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     8095..8115
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     8266..8285
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        10235..10310
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    10358..10475
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tcgtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgggc ctcggcggcc aagcttggca atccggtact gttggtaaag
      841 ccaccatggc caagcctttg tctcaagaag aatccaccct cattgaaaga gcaacggcta
      901 caatcaacag catccccatc tctgaagact acagcgtcgc cagcgcagct ctctctagcg
      961 acggccgcat cttcactggt gtcaatgtat atcattttac tgggggacct tgtgcagaac
     1021 tcgtggtgct gggcactgct gctgctgcgg cagctggcaa cctgacttgt atcgtcgcga
     1081 tcggaaatga gaacaggggc atcttgagcc cctgcggacg gtgccgacag gtgcttctcg
     1141 atctgcatcc tgggatcaaa gccatagtga aggacagtga tggacagccg acggcagttg
     1201 ggattcgtga attgctgccc tctggttatg tgtgggaggg cggcagcgga gcaaccaatt
     1261 ttagtctcct caaacaggcc ggcgatgtcg aagaaaatcc gggcccatac ccatacgatg
     1321 ttccagatta cgcttatccc tacgacgtgc ctgattatgc atacccatat gatgtccccg
     1381 actatgccgg atccatggga aagtctcttt ctcatttgcc tttgcattca agcaaagaag
     1441 atgcttatga tggtgtaacg tcagagaata tgcgcaatgg actggttaat agtgaagtcc
     1501 ataatgaaga tggaagaaat ggagatgtct ctcagtttcc atatgtggaa tttacaggaa
     1561 gagatagtgt cacctgccct acttgtcagg gtactggtcg tatcccacgc gggcaagaaa
     1621 accaactggt ggcattgatt ccatatagtg atcagagatt aaggccaaga agaacaaagc
     1681 tgtatgtgat ggcttctgtg tttgtctgtc tactcctttc tggattggct gtgtttttcc
     1741 ttttccctcg gtcaattgat gtcaagtaca ttggtgtaaa atcagcctat gtcagttatg
     1801 atgttcagaa gcgtacaatt tatttaaata tcacaaacac actaaatata acaaacaata
     1861 actattactc tgtcgaagtt gaaaacatca ctgcccaagt tcaattttcg aagactgtaa
     1921 tcggcaaagc acgcttaaac aacataacca ttattggtcc acttgatatg aaacaaattg
     1981 attacacagt acctaccgtt atagcagagg aaatgagtta tatgtatgat ttctgtactc
     2041 tgatatccat caaagtgcat aacatagtac tcatgatgca agttactgtg acaacaacat
     2101 actttggcca ctctgaacag atatcccagg agaggtatca gtatgtcgac tgtggaagaa
     2161 acacaactta tcagttgggg cagtctgaat atttaaatgt acttcagcca caacagtgaa
     2221 cgcgttaagt cgacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     2281 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     2341 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     2401 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     2461 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
     2521 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     2581 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct
     2641 ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
     2701 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
     2761 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
     2821 cgcgtcgact ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa
     2881 agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc tgctttttgc
     2941 ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     3001 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     3061 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     3121 ctctagcagt acgtatagta gttcatgtca tcttattatt cagtatttat aacttgcaaa
     3181 gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata
     3241 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     3301 tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact
     3361 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     3421 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
     3481 tgaggaggct tttttggagg cctagggacg tacccaattc gccctatagt gagtcgtatt
     3541 acgcgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc
     3601 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc
     3661 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgggac gcgccctgta
     3721 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     3781 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     3841 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     3901 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
     3961 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     4021 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
     4081 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta
     4141 acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     4201 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     4261 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     4321 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     4381 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     4441 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     4501 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     4561 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     4621 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     4681 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     4741 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     4801 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     4861 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     4921 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     4981 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     5041 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     5101 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     5161 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     5221 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     5281 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     5341 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     5401 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     5461 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     5521 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     5581 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     5641 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     5701 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     5761 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     5821 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     5881 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     5941 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     6001 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     6061 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct
     6121 ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc
     6181 gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt
     6241 acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac
     6301 aggaaacagc tatgaccatg attacgccaa gcgcgcaatt aaccctcact aaagggaaca
     6361 aaagctggag ctgcaagctt aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg
     6421 gtaacgatga gttagcaaca tgccttacaa ggagagaaaa agcaccgtgc atgccgattg
     6481 gtggaagtaa ggtggtacga tcgtgcctta ttaggaaggc aacagacggg tctgacatgg
     6541 attggacgaa ccactgaatt gccgcattgc agagatattg tatttaagtg cctagctcga
     6601 tacataaacg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     6661 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     6721 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     6781 atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct
     6841 ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg
     6901 tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg
     6961 tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg
     7021 gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg
     7081 cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac
     7141 aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc
     7201 tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag
     7261 aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccgctgat cttcagacct
     7321 ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa
     7381 attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa
     7441 agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg
     7501 ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag
     7561 cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc
     7621 tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa
     7681 cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg
     7741 aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag
     7801 tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa
     7861 aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg
     7921 aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga
     7981 ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag
     8041 ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc cagagagggc
     8101 ctatttccca tgattccttc atatttgcat atacgataca aggctgttag agagataatt
     8161 agaattaatt tgactgtaaa cacaaagata ttagtacaaa atacgtgacg tagaaagtaa
     8221 taatttcttg ggtagtttgc agttttaaaa ttatgtttta aaatggacta tcatatgctt
     8281 accgtaactt gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgaggt
     8341 accggtaggc aggtgtgttg taaatgagca cacaaaatac acatgctaaa atattatatt
     8401 ctatgacctt tataaaatca accaaaatct tctttttaat aactttagta tcaataatta
     8461 gaatttttat gttccttttt gcaaactttt aataaaaatg agcaaaataa aaaaacgcta
     8521 gttttagtaa ctcgcgttgt tttcttcacc tttaataata gctactccac cacttgttcc
     8581 taagcggtca gctcctgctt caatcatttt ttgagcatct tcaaatgttc taactccacc
     8641 agctgcttta actaaagcat tgtctttaac aactgacttc attagtttaa catcttcaaa
     8701 tgttgcacct gattttgaaa atcctgttga tgttttaaca aattctaatc cagcttcaac
     8761 agctatttca caagctttca tgatttcttc ttttgttaat aaacaatttt ccataataca
     8821 tttaacaaca tgtgatccag ctgctttttt tacagctttc atgtcttcta aaactaattc
     8881 ataatttttg tcttttaatg caccaatatt taataccata tcaatttctg ttgcaccatc
     8941 tttaattgct tcagaaactt cgaatgcttt tgtagctgtt gtgcatgcac ctagaggaaa
     9001 acctacaaca tttgttattc ctacatttgt gccttttaat aattctttac aatagcttgt
     9061 tcaatatgaa ttaacacaaa ctgttgcaaa atcaaattca attgcttcat cacataattg
     9121 tttaatttca gctttcgtag catcttgttt taataatgtg tgatctatat atttgtttag
     9181 tttcattttt tctcctatat attcattttt aattttaatt ctttaataat ttcgtctact
     9241 ttaactttag cgttttgaac agattcacca acacctataa aataaatttt tagtttaggt
     9301 tcagttccac ttgggcgaac agcaaatcat gacttatctt ctaaataaaa ttttagtaag
     9361 tcttgtcctg gcatattata cattccatcg atgtagtctt caacattaac aactttaagt
     9421 ccagcaattt gagttaaggg tgttgctctc aatgatttca ttaatggttc aatttttaat
     9481 ttcttttctt ctggtttaaa attcaagttt aaagtgaaag tgtaatatgc acccatttct
     9541 ttaaataaat cttctaaata gtctactaat gttttatttt gttttttata aaatcaagca
     9601 gcctctgcta ttaatataga agcttgtatt ccatctttat ctctagctga gtcatcaatt
     9661 acatatccat aactttcttc ataagcaaaa acaaaattta atccgttatc ttcttcttta
     9721 gcaatttctc tacccattca tttaaatcca gttaaagttt ttacaatatt aactccatat
     9781 ttttcatgag cgattctatc acccaaatca cttgttacaa aacttgaata tagagccgga
     9841 ttttttggaa tgctatttaa gcgttttaga tttgataatt ttcaatcaat taaaattggt
     9901 cctgtttgat ttccatctaa tcttacaaaa tgaccatcat gttttattgc cattccaaat
     9961 ctgtcagcat ctgggtcatt cataataata atatctgcat catgtttaat accatattca
    10021 agcggtattt ttcatgcagg atcaaattct ggatttggat ttacaacatt tttaaatgtt
    10081 tcatcttcaa atgcatgctc ttcaacctca ataacgttat atcctgattc acgtaatatt
    10141 tttggggtaa atttagttcc tgttccatta actgcgctaa aaataatttt taaatctttt
    10201 ttagcttctt gctctttttt gtagacctgc agaggtttta gagctagaaa tagcaagtta
    10261 aaataaggct agtccgttat caacttgaaa aagtggcacc gagtcggtgc ttttttgaat
    10321 tctagatctt gagacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg
    10381 gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag
    10441 aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag
    10501 atccactttg gcgccggctc gaggggg
//
