LOCUS       sequence-476576-        9072 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human DDX6-NT fused to EGFP in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9072)
  TITLE     Su lab plasmids 2025
REFERENCE   2  (bases 1 to 9072)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: LLPS-pCDH-DDX6-NT
FEATURES             Location/Qualifiers
     source          1..9072
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     180..202
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     194..211
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     195..211
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        226..452
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             453..633
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    680..805
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1298..1531
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1716..1760
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2027..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2233..2436
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2387..2407
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2433..2457
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2510..2527
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             2540..2557
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             join(3485..3487,3488..3490,3491..4204)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3530..3551)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3791..3810)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4138..4159
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        4246..4457
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             4470..4738
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             4765..5364
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4765..4784)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5261..5281
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5371..5959
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5424..5444)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5842..5853)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6033..6266
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6338..6459
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6375..6394)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6429..6448
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6499..6634
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6667..6683)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6667..6683)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6680..6702)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6691..6707
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6715..6721,6722..6739,6740..6745))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6760..6781
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6898..6915)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7069..7657)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(7828..8619,8620..8688))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8451..8470
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8689..8793)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8861..8879
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8917..8939)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     9039..9058
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc
       61 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
      121 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
      181 ccagtcacga cgttgtaaaa cgacggccag tgccaagctg acgcgtgtag tcttatgcaa
      241 tactcttgta gtcttgcaac atggtaacga tgagttagca acatgcctta caaggagaga
      301 aaaagcaccg tgcatgccga ttggtggaag taaggtggta cgatcgtgcc ttattaggaa
      361 ggcaacagac gggtctgaca tggattggac gaaccactga attgccgcat tgcagagata
      421 ttgtatttaa gtgcctagct cgatacataa acgggtctct ctggttagac cagatctgag
      481 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
      541 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
      601 gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc
      661 gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc
      721 aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa
      781 ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga
      841 aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc
      901 aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg
      961 tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc
     1021 attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac
     1081 caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca
     1141 agcggccact gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat
     1201 tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga
     1261 gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct
     1321 tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac
     1381 aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac
     1441 agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg
     1501 tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca
     1561 tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt
     1621 ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac
     1681 actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat
     1741 tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa
     1801 aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt
     1861 ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa
     1921 ccccgagggg acccgacagg cccgaaggaa tagaagaaga aggtggagag agagacagag
     1981 acagatccat tcgattagtg aacggatctc gacggtatcg gttaactttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aattcaaaat tttatcgata ctagtattat
     2161 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
     2221 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac
     2281 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
     2341 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt
     2401 aggcgtgtac ggtgggaggt ttatataagc agagctcgtt tagtgaaccg tcagatcgcc
     2461 tggagacgcc atccacgctg ttttgacctc catagaagat tctagaatgc atcatcacca
     2521 tcaccacgag ccaaccactc tggttccgcg tggatccgat aacgcgagca cggccagaac
     2581 agagaaccct gttataatgg gtctgtccag tcaaaatggt cagctgagag gccctgtgaa
     2641 acccactggt ggccctggag gagggggcac acagacacag caacagatga accagctgaa
     2701 aaacaccaac acaatcaata atggcactca gcagcaagca cagagtatga ccaccactat
     2761 taaacctggt gatgactgga aaaagacttt aaaactccct ccaaaggatc taagaatcaa
     2821 aacttcggat gtgacctcca caaaaggaaa tgagtttgaa gattactgtt tgaaacggga
     2881 gttactgatg ggaatttttg aaatgggctg ggaaaagcca tctcctattc aggaggagag
     2941 cattcccatt gctttatctg gtagggatat cttagctaga gcaaaaaatg gaacaggcaa
     3001 gagcggtgcc tacctcattc ccttacttga acggctagac ctgaagaagg acaatataca
     3061 agcaatggtg attgttccca ctagagaact tgctctacag gtcagtcaaa tttgcatcca
     3121 ggtcagcaaa cacatgggag gggccaaagt gatggcaacc acaggaggaa ccaatttacg
     3181 agatgacata atgaggcttg atgatacagt gcacgtggtg attgctaccc ctgggagaat
     3241 cctggatctt attaagaaag gagtagcaaa ggttgatcat gtccagatga tagtattgga
     3301 tgaggcagat aagttgctgt cacaggattt tgtgcagata atggaggata ttattctcac
     3361 gctacctaaa aacaggcaga ttttactata ttccgctact ttccctctta gtgtacagaa
     3421 gttcatgaat tcccatttgc agaaacccta tgagattaac ctgatggagg aagagccaac
     3481 cactatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
     3541 ggacggcgac gtaaacggcc acaagttcag cgtgtctggc gagggcgagg gcgatgccac
     3601 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
     3661 caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat
     3721 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
     3781 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
     3841 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
     3901 gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa
     3961 gaacggcatc aaggcgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct
     4021 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
     4081 ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat
     4141 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa
     4201 gtaagcggcc gcgaaggatc tgcgatcgct ccggtgcccg tcagtgggca gagcgcacat
     4261 cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgaacgggt gcctagagaa
     4321 ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg
     4381 gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt
     4441 ttgccgccag aacacagctg aagcttcgag gggctcgcat ctctccttca cgcgcccgcc
     4501 gccctacctg aggccgccat ccacgccggt tgagtcgcgt tctgccgcct cccgcctgtg
     4561 gtgcctcctg aactgcgtcc gccgtctagg taagtttaaa gctcaggtcg agaccgggcc
     4621 tttgtccggc gctcccttgg agcctaccta gactcagccg gctctccacg ctttgcctga
     4681 ccctgcttgc tcaactctac gtctttgttt cgttttctgt tctgcgccgt tacagatcca
     4741 agctgtgacc ggcgcctacg ctagatgacc gagtacaagc ccacggtgcg cctcgccacc
     4801 cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc
     4861 acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc
     4921 ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg
     4981 gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc
     5041 ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc
     5101 ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc
     5161 gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag
     5221 cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag
     5281 cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc
     5341 atgacccgca agcccggtgc ctgagtcgac aatcaacctc tggattacaa aatttgtgaa
     5401 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     5461 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     5521 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     5581 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     5641 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     5701 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     5761 gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg
     5821 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     5881 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
     5941 ctttgggccg cctccccgcc tggtaccttt aagaccaatg acttacaagg cagctgtaga
     6001 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag
     6061 ataagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga
     6121 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     6181 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     6241 ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt
     6301 ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa
     6361 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     6421 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc
     6481 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     6541 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     6601 ttccagaagt agtgaggagg cttttttgga ggcctagact tttgcagaga ccaaattcgt
     6661 aatcatgtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     6721 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     6781 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     6841 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     6901 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     6961 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     7021 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     7081 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     7141 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     7201 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     7261 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     7321 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     7381 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     7441 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     7501 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     7561 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     7621 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     7681 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     7741 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     7801 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     7861 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     7921 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     7981 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     8041 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     8101 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     8161 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     8221 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     8281 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     8341 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     8401 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     8461 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     8521 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     8581 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     8641 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     8701 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     8761 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
     8821 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc
     8881 ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga
     8941 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc
     9001 agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact
     9061 gagagtgcac ca
//
