LOCUS       sequence-47658-e         893 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 893)
  AUTHORS   Bergink S, Salomons FA, Hoogstraten D, Groothuis TA, de Waard H, Wu 
            J, Yuan L, Citterio E, Houtsmuller AB, Neefjes J, Hoeijmakers JH, 
            Vermeulen W, Dantuma NP
  TITLE     DNA damage triggers nucleotide excision repair-dependent 
            monoubiquitylation of histone H2A.
  JOURNAL   Genes Dev. 2006 May 15. 20(10):1343-52.
  PUBMED    16702407
REFERENCE   2  (bases 1 to 893)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            2006 May 15. 20(10):1343-52."
COMMENT     Sequence Label: GFP-Ub KO
FEATURES             Location/Qualifiers
     source          1..893
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             53..280
                     /codon_start=1
                     /product="human ubiquitin variant in which all of the 
                     lysines have been mutated to arginine"
                     /label=ubiquitin-K0
                     /note="also known as ubiquitin-KO"
                     /translation="MQIFVRTLTGRTITLEVEPSDTIENVRARIQDREGIPPDQQRLIF
                     AGRQLEDGRTLSDYNIQRESTLHLVLRLRGG"
     polyA_signal    428..549
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(465..484)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     519..538
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(693..714)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ggacgagctg tacaagtccg gactcagatc tcgagctcaa gcttatatac atatgcagat
       61 cttcgtcaga acgttaaccg gtagaaccat aactctagaa gttgaaccat ccgataccat
      121 cgaaaacgtt agagctagaa ttcaagacag agaaggcatt ccacctgatc aacaaagatt
      181 gatctttgcc ggtagacagc tcgaggacgg tagaacgctg tctgattaca acattcagag
      241 agagtcgacc ttacatcttg tcttaagact aagaggtggt tgaggtaccg cgggcccggg
      301 atccaccgga tctagataac tgatcataat cagccatacc acatttgtag aggttttact
      361 tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt
      421 tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
      481 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
      541 tgtatcttaa cgcgtaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt
      601 gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa
      661 aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa
      721 agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac
      781 gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga
      841 accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac gtg
//
