LOCUS       sequence-476581-        2250 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BsaI Golden Gate assembly plasmid encoding the synZiFTR ZF10.BSx8 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2250)
  AUTHORS   Peterman EL, Ploessl DS, Love KS, Sanabria V, Daniels RF, Johnstone 
            CP, Godavarti DR, Kabaria SR, Oakes CG, Pai AA, Galloway KE
  TITLE     High-resolution profiling reveals coupled transcriptional and 
            translational regulation of transgenes.
  JOURNAL   Nucleic Acids Research, Volume 53, Issue 11, 24 June 2025, gkaf528
  PUBMED    39651241
REFERENCE   2  (bases 1 to 2250)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Research, Volume 53, Issue 11, 24 June 2025, gkaf528"
COMMENT     Sequence Label: pKG3657_pPV1-ZF10.BSx8
FEATURES             Location/Qualifiers
     source          1..2250
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      49..637
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     538..557
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        1163..1267
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1268..1336,1337..2128)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGYRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1486..1505)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
       61 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
      121 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
      181 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
      241 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
      301 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
      361 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
      421 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
      481 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
      541 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
      601 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg ggtctcatac
      661 tgacgattga gtaacactgt atctagtcta cctacagggc tcggcgtagc cgatgtcgcg
      721 cggatagcgt caacctcttc ggcgtagccg atgtcgcgca atcagttctc ccgaggtcgg
      781 cgtagccgat gtcgcgcaga gccgtgcttc caatacggcg tagccgatgt cgcgccgggt
      841 ttcgtaacaa tcgcatgagg attcgcaacg ccttcggcgt agccgatgtc gcgctcccgt
      901 ctcagtaaag gtcggcgtag ccgatgtcgc gcaatcggac tgccttcgta cggcgtagcc
      961 gatgtcgcgc gtatcagtcg cctcggaacg gcgtagccga tgtcgcgcat tcgtaagagg
     1021 ctcactctcc cttacacgga gtggatagga tcctctagag ggtatataat gggggccact
     1081 agtctactac cagagctcat cgctagcgct accggatcaa tgagagacct agacgtcagg
     1141 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     1201 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     1261 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     1321 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     1381 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     1441 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     1501 attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     1561 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     1621 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     1681 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     1741 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     1801 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     1861 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     1921 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     1981 tgggtatcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     2041 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     2101 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     2161 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     2221 tctcatgacc aaaatccctt aacgtgagtt
//
