LOCUS       sequence-476588-        9336 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human DDX6-Delta-IDR fused to EGFP in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9336)
  TITLE     Su lab plasmids 2025
REFERENCE   2  (bases 1 to 9336)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: LLPS-pCDH-DDX6-Delta-IDR
FEATURES             Location/Qualifiers
     source          1..9336
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     180..202
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     194..211
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     195..211
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        226..452
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             453..633
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    680..805
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1298..1531
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1716..1760
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2027..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2233..2436
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2387..2407
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2433..2457
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2510..2527
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             2540..2557
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             join(3749..3751,3752..3754,3755..4468)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3794..3815)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4055..4074)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4402..4423
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        4510..4721
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             4734..5002
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             5029..5628
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5029..5048)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5525..5545
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5635..6223
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5688..5708)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6106..6117)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6297..6530
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6602..6723
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6639..6658)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6693..6712
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6763..6898
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6931..6947)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6931..6947)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6944..6966)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6955..6971
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6979..6985,6986..7003,7004..7009))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7024..7045
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7162..7179)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7333..7921)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(8092..8883,8884..8952))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8715..8734
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8953..9057)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9125..9143
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(9181..9203)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     9303..9322
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc
       61 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
      121 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
      181 ccagtcacga cgttgtaaaa cgacggccag tgccaagctg acgcgtgtag tcttatgcaa
      241 tactcttgta gtcttgcaac atggtaacga tgagttagca acatgcctta caaggagaga
      301 aaaagcaccg tgcatgccga ttggtggaag taaggtggta cgatcgtgcc ttattaggaa
      361 ggcaacagac gggtctgaca tggattggac gaaccactga attgccgcat tgcagagata
      421 ttgtatttaa gtgcctagct cgatacataa acgggtctct ctggttagac cagatctgag
      481 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
      541 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
      601 gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc
      661 gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc
      721 aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa
      781 ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga
      841 aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc
      901 aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg
      961 tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc
     1021 attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac
     1081 caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca
     1141 agcggccact gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat
     1201 tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga
     1261 gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct
     1321 tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac
     1381 aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac
     1441 agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg
     1501 tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca
     1561 tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt
     1621 ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac
     1681 actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat
     1741 tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa
     1801 aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt
     1861 ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa
     1921 ccccgagggg acccgacagg cccgaaggaa tagaagaaga aggtggagag agagacagag
     1981 acagatccat tcgattagtg aacggatctc gacggtatcg gttaactttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aattcaaaat tttatcgata ctagtattat
     2161 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
     2221 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac
     2281 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
     2341 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt
     2401 aggcgtgtac ggtgggaggt ttatataagc agagctcgtt tagtgaaccg tcagatcgcc
     2461 tggagacgcc atccacgctg ttttgacctc catagaagat tctagaatgc atcatcacca
     2521 tcaccacgag ccaaccactc tggttccgcg tggatccgat aacgcgaaag gaaatgagtt
     2581 tgaagattac tgtttgaaac gggagttact gatgggaatt tttgaaatgg gctgggaaaa
     2641 gccatctcct attcaggagg agagcattcc cattgcttta tctggtaggg atatcttagc
     2701 tagagcaaaa aatggaacag gcaagagcgg tgcctacctc attcccttac ttgaacggct
     2761 agacctgaag aaggacaata tacaagcaat ggtgattgtt cccactagag aacttgctct
     2821 acaggtcagt caaatttgca tccaggtcag caaacacatg ggaggggcca aagtgatggc
     2881 aaccacagga ggaaccaatt tacgagatga cataatgagg cttgatgata cagtgcacgt
     2941 ggtgattgct acccctggga gaatcctgga tcttattaag aaaggagtag caaaggttga
     3001 tcatgtccag atgatagtat tggatgaggc agataagttg ctgtcacagg attttgtgca
     3061 gataatggag gatattattc tcacgctacc taaaaacagg cagattttac tatattccgc
     3121 tactttccct cttagtgtac agaagttcat gaattcccat ttgcagaaac cctatgagat
     3181 taacctgatg gaggaactaa ctctgaaggg agtaacccag tactacgcat atgtaactga
     3241 gcgccaaaaa gtacactgcc tcaacacact tttctccagg cttcagataa accagtcgat
     3301 cattttctgt aactcctctc agcgagttga attgctagcc aagaagattt ctcaactggg
     3361 ttattcttgc ttctatattc atgctaaaat gaggcaggaa catcgaaatc gtgtatttca
     3421 tgatttccga aatggcttat gccgcaatct tgtttgcact gatctgttta cccgaggtat
     3481 tgatatacaa gctgtgaatg tggtaataaa ctttgatttc ccaaagctgg cagagaccta
     3541 tctccatcgt attggaagat caggtcgctt tggtcatctt ggcttagcca tcaacttgat
     3601 cacatatgat gatcgcttca acctgaaaag tattgaggag cagctgggaa cagaaattaa
     3661 acctattccg agcaacattg ataagagcct gtatgtggca gaataccaca gcgagcctgt
     3721 agaagatgag aaacctgagc caaccactat ggtgagcaag ggcgaggagc tgttcaccgg
     3781 ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc
     3841 tggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac
     3901 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg
     3961 cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga
     4021 aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc
     4081 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt
     4141 caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt
     4201 ctatatcatg gccgacaagc agaagaacgg catcaaggcg aacttcaaga tccgccacaa
     4261 catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga
     4321 cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga
     4381 ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac
     4441 tctcggcatg gacgagctgt acaagtaagc ggccgcgaag gatctgcgat cgctccggtg
     4501 cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg
     4561 gcaattgaac gggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt
     4621 actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg
     4681 tgaacgttct ttttcgcaac gggtttgccg ccagaacaca gctgaagctt cgaggggctc
     4741 gcatctctcc ttcacgcgcc cgccgcccta cctgaggccg ccatccacgc cggttgagtc
     4801 gcgttctgcc gcctcccgcc tgtggtgcct cctgaactgc gtccgccgtc taggtaagtt
     4861 taaagctcag gtcgagaccg ggcctttgtc cggcgctccc ttggagccta cctagactca
     4921 gccggctctc cacgctttgc ctgaccctgc ttgctcaact ctacgtcttt gtttcgtttt
     4981 ctgttctgcg ccgttacaga tccaagctgt gaccggcgcc tacgctagat gaccgagtac
     5041 aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc
     5101 gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag
     5161 cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg
     5221 tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg
     5281 ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc
     5341 gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc
     5401 ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg
     5461 ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg
     5521 ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg
     5581 cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgagt cgacaatcaa
     5641 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     5701 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     5761 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     5821 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     5881 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     5941 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     6001 actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt
     6061 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     6121 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     6181 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctggtac ctttaagacc
     6241 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga
     6301 agggctaatt cactcccaac gaagataaga tctgcttttt gcttgtactg ggtctctctg
     6361 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     6421 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     6481 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc
     6541 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag
     6601 gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     6661 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     6721 ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc cctaactccg
     6781 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     6841 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     6901 gacttttgca gagaccaaat tcgtaatcat gtcatagctg tttcctgtgt gaaattgtta
     6961 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     7021 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     7081 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     7141 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     7201 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     7261 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     7321 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     7381 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     7441 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     7501 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     7561 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     7621 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     7681 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     7741 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     7801 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     7861 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     7921 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     7981 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     8041 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     8101 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     8161 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     8221 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     8281 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     8341 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     8401 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     8461 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     8521 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     8581 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     8641 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     8701 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     8761 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     8821 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     8881 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     8941 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     9001 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     9061 atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta
     9121 taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa
     9181 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag
     9241 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta
     9301 tgcggcatca gagcagattg tactgagagt gcacca
//
