LOCUS       sequence-476590-        9810 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Orthogonal dual Cas9 gRNA and BFP reporter lentivirus cassette 
            plasmid. For use with S. pyogenes and S. aureus gRNAs and includes a
            BFP reporter which reports on C-to-T editing..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9810)
  TITLE     Elucidating the genetic mechanisms governing cytosine base editing 
            outcomes through CRISPRi screens
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9810)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pSG297-(Sp-gRNA)-(Sa-gRNA)-(PuroR_T2A_BFP(C>T
            ...
FEATURES             Location/Qualifiers
     source          1..9810
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     90..109
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(328..432)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(507..526)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        698..1077
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1079..1277
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1232..1252
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             1295..1475
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1522..1647
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2144..2377
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2562..2606
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2904..3021
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    complement(3274..3307)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     3311..3331
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     promoter        3821..4069
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     3821..3841
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     3992..4011
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     promoter        4491..4702
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          4703..5641
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5598..5618
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             5662..6258
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5662..5681)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6158..6178
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             6268..6321
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(6334..6336,6337..7047)
                     /codon_start=1
                     /product="strongly enhanced blue fluorescent protein 
                     (Kremers et al., 2007)"
                     /label=SBFP2
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLSHGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(6376..6397)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     6984..7005
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(7070..7103)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    7159..7747
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7212..7232)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7630..7641)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7750..7766)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7751..7767)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             8276..8456
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(8518..9106)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8598..8617)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9277..9810,1..258,259..327))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
       61 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
      121 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
      181 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
      241 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
      301 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
      361 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
      421 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat cgggagatct
      481 cccgatcccc tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag
      541 tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa aatttaagct
      601 acaacaaggc aaggcttgac cgacaattgc atgaagaatc tgcttagggt taggcgtttt
      661 gcgctgcttc gcgatgtacg ggccagatat acgcgttgac attgattatt gactagttat
      721 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca
      781 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
      841 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
      901 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
      961 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
     1021 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg
     1081 atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca
     1141 agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt
     1201 ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg
     1261 gaggtctata taagcagcgc gttttgcctg tactgggtct ctctggttag accagatctg
     1321 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     1381 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     1441 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
     1501 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
     1561 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
     1621 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
     1681 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
     1741 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
     1801 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
     1861 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
     1921 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
     1981 caagcggccg gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa
     2041 gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg
     2101 caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg
     2161 ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg
     2221 ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg
     2281 cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc
     2341 tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa
     2401 aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac
     2461 agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct
     2521 taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat
     2581 tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt
     2641 atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg
     2701 tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc
     2761 tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag
     2821 acagagacag atccattcga ttagtgaacg gatcggcact gcgtgcgcca attctgcaga
     2881 caaatggcag tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca
     2941 ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa
     3001 attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc agtttggtta
     3061 gtaccgggcc cgctctagag atccgacgcg ccatctctag gcccgcgccg gccccctcgc
     3121 acggacttgt gggagaagct cggctactcc cctgccccgg ttaatttgca tataatattt
     3181 cctagtaact atagaggctt aatgtgcgat aaaagacaga taatctgttc tttttaatac
     3241 tagctacatt ttacatgata ggcttggatt tctataactt cgtatagcat acattatacg
     3301 aagttataaa cagcacaaaa ggaaactcac cctaactgta aagtaattgt gtgttttgag
     3361 actataagta tcccttggag aaccaccttg ttggaccagg atgggcacca cccgtttaag
     3421 agctaagctg gaaacagcat agcaagttta aataaggcta gtccgttatc aacttgaaaa
     3481 agtggcaccg agtcggtgct ttttttctcg agtactagga tccattaggc gaggagcatc
     3541 gcccttcccc ggccctcagg taagaggacc aaataccgta gccgtttcca atttcagtcc
     3601 tttagcgcca cctggtgcta actactctat cacgctttta tccaataact acctttgtaa
     3661 atttcctttc aaaagttctg gccgggcgcg gtggcaacgg tgcccgtcag tgcgcacatt
     3721 tccccgaaaa gtgccacctg acgtcgctag ctgtacaaaa aagcaggctt taaaggaacc
     3781 aattcagtcg actggatccg gtaccaaggt cgggcaggaa gagggcctat ttcccatgat
     3841 tccttcatat ttgcatatac gatacaaggc tgttagagag ataattagaa ttaatttgac
     3901 tgtaaacaca aagatattag tacaaaatac gtgacgtaga aagtaataat ttcttgggta
     3961 gtttgcagtt ttaaaattat gttttaaaat ggactatcat atgcttaccg taacttgaaa
     4021 gtatttcgat ttcttggctt tatatatctt gtggaaagga cgaaacacct gagacgatct
     4081 aacgtctctg ttatagtact ctgtaatgaa aattacagaa tctactataa caaggcaaaa
     4141 tgccgtgttt atctcgtcaa cttgttggcg agattttttt aagcttgggc cgctcgaggt
     4201 acctctctac atatgaccac acactggctt aagatgacgg gaccctgtaa acgtgtccgc
     4261 cttgccactt tgtgcaatat ttagctcctt cagtagaggg cgctgcagga acatgtcagg
     4321 agggggcctt ctcttccgga ttctggattc tggtatacac ttggagcgtg gcatttgggg
     4381 gacagccact ggtgcgcagc cccatcaagt tttggtggca tccctaacgc catatatgga
     4441 gttccggccg cgtggataac cgtattaccg ccatgcatgt gcccgtcagt gggcagagcg
     4501 cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa ccggtgccta
     4561 gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc gcctttttcc
     4621 cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa
     4681 cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc ctggcctctt
     4741 tacgggttat ggcccttgcg tgccttgaat tacttccacc tggctgcagt acgtgattct
     4801 tgatcccgag cttcgggttg gaagtgggtg ggagagttcg aggccttgcg cttaaggagc
     4861 cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc gcgtgcgaat
     4921 ctggtggcac cttcgcgcct gtctcgctgc tttcgataag tctctagcca tttaaaattt
     4981 ttgatgacct gctgcgacgc tttttttctg gcaagatagt cttgtaaatg cgggccaaga
     5041 tctgcacact ggtatttcgg tttttggggc cgcgggcggc gacggggccc gtgcgtccca
     5101 gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg gacgggggta
     5161 gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta tcgccccgcc
     5221 ctgggcggca aggctggccc ggtcggcacc agttgcgtga gcggaaagat ggccgcttcc
     5281 cggccctgct gcagggagct caaaatggag gacgcggcgc tcgggagagc gggcgggtga
     5341 gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat gtgactccac
     5401 ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg cgcttttgga gtacgtcgtc
     5461 tttaggttgg ggggaggggt tttatgcgat ggagtttccc cacactgagt gggtggagac
     5521 tgaagttagg ccagcttggc acttgatgta attctccttg gaatttgccc tttttgagtt
     5581 tggatcttgg ttcattctca agcctcagac agtggttcaa agtttttttc ttccatttca
     5641 ggtgtcgtga gctagcccac catgaccgag tacaagccca cggtgcgcct cgccacccgc
     5701 gacgacgtcc cccgggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg
     5761 cgccacaccg tcgacccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc
     5821 ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg
     5881 gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg
     5941 cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg
     6001 gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggagt ctcgcccgac
     6061 caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc
     6121 gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg
     6181 ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg
     6241 acccgcaagc ccggtgccgg atcgggagag ggcagaggaa gtctgctaac atgcggtgac
     6301 gtcgaggaga atcctggccc accggtcgcc actgtgagca agggcgagga gctgttcacc
     6361 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg
     6421 cgcggcgagg gcgagggcga tgccaccaac ggcaagctga ccctgaagtt catctgcacc
     6481 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgagtca tggtgtgcag
     6541 tgcttcagtc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc
     6601 gaaggctacg tccaggagcg caccatctcc ttcaaggacg acggcaccta caagacccgc
     6661 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac
     6721 ttcaaggagg acggcaacat cctggggcac aagctggagt acaacttcaa cagccacaac
     6781 gtctatatca cggccgacaa gcagaagaac ggcatcaagg cgaacttcaa gatccgccac
     6841 aacgtcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc
     6901 gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccaa actgagcaaa
     6961 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc
     7021 actctcggca tggacgagct gtacaagtag gaattcgtcg agggacctaa taacttcgta
     7081 tagcatacat tatacgaagt tatacatgtt taagggttcc ggttccacta ggtacaattc
     7141 gatatcaagc ttatcgataa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     7201 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     7261 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     7321 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     7381 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     7441 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     7501 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg
     7561 tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     7621 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     7681 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     7741 tccccgcatc gataccgtcg acctcgatcg agacctagaa aaacatggag caatcacaag
     7801 tagcaataca gcagctacca atgctgattg tgcctggcta gaagcacaag aggaggagga
     7861 ggtgggtttt ccagtcacac ctcaggtacc tttaagacca atgacttaca aggcagctgt
     7921 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg
     7981 aagacaagat atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattggca
     8041 gaactacaca ccagggccag ggatcagata tccactgacc tttggatggt gctacaagct
     8101 agtaccagtt gagcaagaga aggtagaaga agccaatgaa ggagagaaca cccgcttgtt
     8161 acaccctgtg agcctgcatg ggatggatga cccggagaga gaagtattag agtggaggtt
     8221 tgacagccgc ctagcatttc atcacatggc ccgagagctg catccggact gtactgggtc
     8281 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     8341 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     8401 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagcat
     8461 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     8521 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     8581 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     8641 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     8701 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     8761 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     8821 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     8881 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     8941 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     9001 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     9061 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     9121 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     9181 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     9241 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     9301 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     9361 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     9421 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     9481 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     9541 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     9601 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     9661 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     9721 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     9781 ttctcttact gtcatgccat ccgtaagatg
//
