LOCUS       sequence-476605-       13147 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This all-in-one vector is used to specifically silence the GhPDS 
            gene via virus-induced gene silencing (VIGS)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13147)
  AUTHORS   Hao Y, Yuan Z, Li Y, Zuo D, Cheng H, Wang Q, Zhang Y, Lv L, Liu J, 
            Song G
  TITLE     An all-in-one plant virus-based vector toolkit for streamlined gene 
            silencing, overexpression, and genome editing.
  JOURNAL   Plant Commun. 2025 May 19:101379. doi: 10.1016/j.xplc.2025.101379.
  PUBMED    40394900
REFERENCE   2  (bases 1 to 13147)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Commun. 2025 May 19:101379. doi: 10.1016/j.xplc.2025.101379."
COMMENT     Sequence Label: pVS-GhPDS
FEATURES             Location/Qualifiers
     source          1..13147
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(6233..6250)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6233..6249)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6242..6264)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    6452..6476
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             7777..8406
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             8835..9908
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      9974..10168
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(10404..10426)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    10512..10652
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     10567..10586
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(10667..10684)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10838..11426)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10918..10937)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(11513..12307)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    12732..12756
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     protein_bind    13024..13045
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(13060..13065,13066..13083,13084..13090)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13098..13114
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     13103..13125
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     13122..13138
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     13122..13138
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aattcgagct cattcgagct ccagaacgat ctcagttagg tcatgggaaa gctgatattc
       61 atccctctga gactctatat aattaaatgc gtttggggga ttagctaatt gagaatccat
      121 atagagaaat atggccgcgc agcgacgccg gttgaagaat atgaacaagg aataagatga
      181 acaaggaaga agatgaacaa gcaataattt aattaattat tgttaataag cataaattat
      241 gaaaataatt ttgatggtaa tgaaagtatt ataaataata tttaggagaa aatattgtta
      301 aggaaatatc tggttatgtt tatttgttaa ggtatatggg tttttataga aacccaggag
      361 cagctatggt aagaaaccca ggactcaaaa aatgcctctc aacttctctc atatgttgcg
      421 gagtctggag tcccatttat accatagaaa gcattggggg tatagcatgg taatttcaca
      481 tcggactcca taccgcggcc atctatataa tattactgat ggccgcgcga agtcccggta
      541 tgccttttca gccgttggat ctggagccgt acctttttcg cgttcccatt ttgcccctgg
      601 agtcccgagc tatttacgac cccctgccac cgagtcccct tcgtggagcg ctcttcgtcc
      661 aacacgtgtc ccaacttttg cgcatttttg actacctgtc gcgcacttga attaaagttg
      721 cccttttatt ttcttccaat tattttgcgg ctgagtagtc tagataattg tgacaactta
      781 gtgagcaagt tgtaggagga ccctatccgt ctgggaaaat aattgggttt tcacgtgtac
      841 caatgggaaa taatatgtat agcctattta atttagaaaa ttaaatagtt ttagtaattc
      901 ctatataata gcgtttacta gtctgggtaa aattcgtata ggaaaaaata tttattattt
      961 tttaaattta acctattgaa aatgtacagt ttaaagagta gacgtggatc gaattacatt
     1021 caagcacgtg gaaactcacg gaccccatta ttcaaacgac attccactgg taagcgttat
     1081 ggaggaagac gactacagcc actgaatgtg aaaagagaag atgaacccag aatgtcgacc
     1141 caacgcatcc acgagaatca atatgggtcg gaatttacaa tggcccacaa cacagccatt
     1201 tcgacctata tctcctatcc taatctgggc aagactgaac ccaatcgaac caggtcgtac
     1261 atcaagctga aacgattgcg tttcaagggc acggtgaaga tcgaacgagt tcctactgac
     1321 gtggctatgg atggttcgat tcccaacacg gaaggggtgt tctcattggt catagtcgtt
     1381 gaccgaaaac ctcatttgac tgcatctgga tgcctacata cgtttgacga actatttggt
     1441 gcgataatcc acagccatgg aaccctgtct atagttcccg cgttgaagga tcgttactac
     1501 atacggcatg taattaaacg ggtactgtcc gtcgagaaag ataacgtcat ggtggatatt
     1561 gaaggatcca ctgctctgtc taataggcgt tttaattgtt gggccagttt taaagatctt
     1621 gatttggatt catgtaaggg tgtttatgac aacataaaca agaatgccct gttagtttat
     1681 tactcatggg tatcggatac tgtgtctaaa gcatccactt atgtatcgta tgaccttgat
     1741 tatattggat aatcaataat aacaggaaaa tttgaataag aaaaaatatt atttaaatat
     1801 ttttggttga ggaccattac aactactctt gatacattct tggaccgttg tcctaaccag
     1861 ttcgtttaat tgggccatag acatggtaat gttggactca gccctatggg cccctactat
     1921 tgatgccgag tcacctggat ccagtacagt ggaccctaac ctgtgcagct ctctgtaagg
     1981 tgtctgttcg ttattggact gtgagtccgc atcttcgtta acgggcccta tggtactcct
     2041 ggtcgcccac gactcccctg gccttatttc tattgggcct ggaagcccaa atctgttcat
     2101 ggatgcggac ctaattagtt tcctttccca tttaccatag tctacatgtg agaaatcaac
     2161 gtctttagcg gagaactgtt tggaaaggat ctttaccgtt ggcgccttga acggaatatc
     2221 gacggaatgt ttggcggtcg ataatttcag cttccctttg aattttgcga agtgggtacg
     2281 ctgttgaaca ttcgtatcag aaactctgta atagagtttc caagggattg ggtctttgag
     2341 agagaagaag gaagctgaaa aatagtggag atctatgtta cacctgatcg gaaaagtcca
     2401 cgacgattgt aatgattcat tatcagtcat cctcttgtcg tgaatctcca ctatcactgt
     2461 accagtcgcg ttaattggca cctgctgcct gtactcgata acgcaatgat cgatcttcat
     2521 gcaactgcga ctgagtctcg ccgttaattg tgccgccgtt gacggaaact gcagaacgat
     2581 ctcagttagg tcatgggaaa gctgatattc atccctctga gactctatat aattaaatgc
     2641 gtttggggga ttagctaatt gagaatccat atagagaaat atggccgcgc agcgacgccg
     2701 gttgaagaat atgaacaagg aataagatga acaaggaaga agatgaacaa gcaataattt
     2761 aattaattat tgttaataag cataaattat gaaaataatt ttgatggtaa tgaaagtatt
     2821 ataaataata tttaggagaa aatattgtta aggaaatatc tggttatgtt tatttgttaa
     2881 ggtatatggg tttttataga aacccaggag cagctatggt aagaaaccca ggactcaaaa
     2941 aatgcctctc aacttctctc atatgttgcg gagtctggag tcccatttat accatagaaa
     3001 gcattggggg tatagcatgg taatttcaca tcggactcca taccgcggcc atctatataa
     3061 tattactgat ggccgcgcga agtcccggta tgccttttca gccgttggat ctggagccgt
     3121 acctttttcg cgttcccatt ttgcccctgg agtcccgagc tatttacgac cccctgccac
     3181 cgagtcccct tcgtggagcg ctcttcgtcc aacacgtgtc ccaacttttg cgcatttttg
     3241 actacctgtc gcgcacttga attaaagttg cccttttatt ttcttccaat tattttgcgg
     3301 ctgagtagtc tagtggatcc cccggagctc cacttgggat aggttaagaa aatatgtctg
     3361 gcttgtaaac gaaaagaatc ggggtttcta ggcatttttt aaatagtgac ccaggactca
     3421 aaaactagct ctcaacttct ctcatatgtt gtggagtctg gagtccttta tatatgttta
     3481 cgctatagaa ggcatttggg gactctcccg ggcccaccat aggactccat accgcggcca
     3541 tctatataat attactgatg gccgcgcgga ttttggagcc cccttttgtc gttagcgcga
     3601 cctttaattc aaattaaagg ttgctttttt ctatcgtccg atcattttgc gcctgactag
     3661 cctagatatt agcaacaact tcgcggctaa gttgttgacc gtcaagtata aattaatggg
     3721 cttatggcct aatgggtata gagcaaaatg gcatgcctgc agactagtgc tttactctga
     3781 tccgcagata tttcatcagg aaagagcttt gcaagttcct tcattgtagc atcaataatt
     3841 tctgagtcac tacatgcaat ccattcttct gctggggcaa aaactaactc caacatggat
     3901 tggtttggat tgtaatattc cttacatgtt acagacatgt cagcataaac acttaaaagc
     3961 gggcttctgc taaagagtag atgatcatag gtgttcttca atttcctatc aaaccagatg
     4021 tgaacgttga taactggaac tccaactaat ttctctaatt tcttgaagta tgaaatctct
     4081 ctccagtctt caggcactag tgaattcgct agcgttaact ggccataatc ctgtgtatgc
     4141 aacgttgaaa attcggatct atttttacga ttcgatcatg aattaataaa atttaaattt
     4201 tattgaatga ttttctagta cataactgac atacgactta tcagttgcga aacgaacagc
     4261 tctaattaca ttgttaagtg aaattacgcc taattcgtct aagtacaaca taaccaactg
     4321 tttaaaccta attaaataag tcgacccaga agctgtcaga gatgtcgtcc agacttggaa
     4381 gttcaggtaa gctttgtgga gatgcaacgc tctccgtaag ttgtggttga atcggatctg
     4441 gacgtggtat atacgcgtcc tggtgtatag cagatcctct actccgtata tcttgaaata
     4501 taggggattt attatctccc agatatacac gccattctct gcctgacgtg cagtgatgat
     4561 ttcccctgtg cgtgaatcca tgaccggtgc agcctatgtg gaagtatatg gtgcacccac
     4621 agtttagatc aatgcgtcgt cgtctaatcg ccctcctctt ggcttgcctg tgtgccttct
     4681 tgatagaggg gggcgtcgag agtgatgaag ataacattct tgagagtcca atgtttgagc
     4741 gacgcatttt ccgctttatc caggaaatct ttatagctgg atccctcgcc tggattgcaa
     4801 agcacgattg aagggatccc tcctttaatt tgaactggtt tcccgtactt gcaatttgac
     4861 tgccagtctt tttgggcccc aagaagctct ttccaatgct ttagctttag gtattgcggt
     4921 gctacgtcat caatgacgtt ataggcaaca ttattcgagt acactcgatt attgaagtcc
     4981 aggtgtccac tgaggtaatt atgtgggcct aaagcacgag cccacatcgt cttgcccgtt
     5041 ctagaatcac cttccactat gagactaact ggtcttacag gccgcgcagc gggacccatt
     5101 ccaaaataat catccgccca ttcttgcatc tgttcaggaa cgttagtgaa agaggaaagt
     5161 ggaaatggag ggacccattg ttcaggcgcc tttttgaaaa ggcgttccaa gttcgctgta
     5221 atgttatggt aactcacgat gaacgccctt ggatctccgg cccggataat tgcaagagct
     5281 tccgccaaac taggtgcatt gatggcgtta taatagacgt cgtctttatt cttctttgta
     5341 cccccaggca ctttgaactg tccggattca caacaatcgc cgtctttggt gatgtaattc
     5401 ttgacggcgt tggcgtcttt ggctgcttgt atgtttgggt gaaaattggt cgaccttctg
     5461 gggtgagtaa ggtcgaaaaa tctcttattc ttgatgttgg actttccaga gagttggatg
     5521 agacagtgaa ggtgtgggaa tccgtccgag tgctcctctc tggcaactcg aatatatgtt
     5581 ggtttgacga ctgaccatga taaggtttga agcatttgaa gagcttcatt ttttgggata
     5641 tcgcacttgg gataggttaa gaaaatatgt ctggcttgta aacgaaaaga atcggggttt
     5701 ctaggcattt tttaaatagt gacccaggac tcaaaaacta gctctcaact tctctcatat
     5761 gttgtggagt ctggagtcct ttatatatgt ttacgctata gaaggcattt ggggactctc
     5821 ccgggcccac cataggactc cataccgcgg ccatctatat aatattactg atggccgcgc
     5881 ggattttgga gccccctttt gtcgttagcg cgacctttaa ttcaaattaa aggttgcttt
     5941 tttctatcgt ccaatcattt tgcgcctgac tagcctagat attagcaaca acttcgcgac
     6001 taagttgttg accgtcaagt ataaattaat gggcttatgg cccaatgggt atagagcaaa
     6061 atgcccaagc gtgatgcccc gtggcgctta ttagcgggta cgtctaaggt aagtcgaaat
     6121 gccaactact ctcccttgag agtattgggc cctaaagtta ataagggacc tcgagggggg
     6181 gcccggtacc cggggatcct ctagagtcga cctgcaggca tgcaagcttg gcactggccg
     6241 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag
     6301 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
     6361 aacagttgcg cagcctgaat ggcgaatgct agagcagctt gagcttggat cagattgtcg
     6421 tttcccgcct tcagtttaaa ctatcagtgt ttgacaggat atattggcgg gtaaacctaa
     6481 gagaaaagag cgtttattag aataatcgga tatttaaaag ggcgtgaaaa ggtttatccg
     6541 ttcgtccatt tgtatgtgca tgccaaccac agggttcccc tcgggatcaa agtactttga
     6601 tccaacccct ccgctgctat agtgcagtcg gcttctgacg ttcagtgcag ccgtcttctg
     6661 aaaacgacat gtcgcacaag tcctaagtta cgcgacaggc tgccgccctg cccttttcct
     6721 ggcgttttct tgtcgcgtgt tttagtcgca taaagtagaa tacttgcgac tagaaccgga
     6781 gacattacgc catgaacaag agcgccgccg ctggcctgct gggctatgcc cgcgtcagca
     6841 ccgacgacca ggacttgacc aaccaacggg ccgaactgca cgcggccggc tgcaccaagc
     6901 tgttttccga gaagatcacc ggcaccaggc gcgaccgccc ggagctggcc aggatgcttg
     6961 accacctacg ccctggcgac gttgtgacag tgaccaggct agaccgcctg gcccgcagca
     7021 cccgcgacct actggacatt gccgagcgca tccaggaggc cggcgcgggc ctgcgtagcc
     7081 tggcagagcc gtgggccgac accaccacgc cggccggccg catggtgttg accgtgttcg
     7141 ccggcattgc cgagttcgag cgttccctaa tcatcgaccg cacccggagc gggcgcgagg
     7201 ccgccaaggc ccgaggcgtg aagtttggcc cccgccctac cctcaccccg gcacagatcg
     7261 cgcacgcccg cgagctgatc gaccaggaag gccgcaccgt gaaagaggcg gctgcactgc
     7321 ttggcgtgca tcgctcgacc ctgtaccgcg cacttgagcg cagcgaggaa gtgacgccca
     7381 ccgaggccag gcggcgcggt gccttccgtg aggacgcatt gaccgaggcc gacgccctgg
     7441 cggccgccga gaatgaacgc caagaggaac aagcatgaaa ccgcaccagg acggccagga
     7501 cgaaccgttt ttcattaccg aagagatcga ggcggagatg atcgcggccg ggtacgtgtt
     7561 cgagccgccc gcgcacgtct caaccgtgcg gctgcatgaa atcctggccg gtttgtctga
     7621 tgccaagctg gcggcctggc cggccagctt ggccgctgaa gaaaccgagc gccgccgtct
     7681 aaaaaggtga tgtgtatttg agtaaaacag cttgcgtcat gcggtcgctg cgtatatgat
     7741 gcgatgagta aataaacaaa tacgcaaggg gaacgcatga aggttatcgc tgtacttaac
     7801 cagaaaggcg ggtcaggcaa gacgaccatc gcaacccatc tagcccgcgc cctgcaactc
     7861 gccggggccg atgttctgtt agtcgattcc gatccccagg gcagtgcccg cgattgggcg
     7921 gccgtgcggg aagatcaacc gctaaccgtt gtcggcatcg accgcccgac gattgaccgc
     7981 gacgtgaagg ccatcggccg gcgcgacttc gtagtgatcg acggagcgcc ccaggcggcg
     8041 gacttggctg tgtccgcgat caaggcagcc gacttcgtgc tgattccggt gcagccaagc
     8101 ccttacgaca tatgggccac cgccgacctg gtggagctgg ttaagcagcg cattgaggtc
     8161 acggatggaa ggctacaagc ggcctttgtc gtgtcgcggg cgatcaaagg cacgcgcatc
     8221 ggcggtgagg ttgccgaggc gctggccggg tacgagctgc ccattcttga gtcccgtatc
     8281 acgcagcgcg tgagctaccc aggcactgcc gccgccggca caaccgttct tgaatcagaa
     8341 cccgagggcg acgctgcccg cgaggtccag gcgctggccg ctgaaattaa atcaaaactc
     8401 atttgagtta atgaggtaaa gagaaaatga gcaaaagcac aaacacgcta agtgccggcc
     8461 gtccgagcgc acgcagcagc aaggctgcaa cgttggccag cctggcagac acgccagcca
     8521 tgaagcgggt caactttcag ttgccggcgg aggatcacac caagctgaag atgtacgcgg
     8581 tacgccaagg caagaccatt accgagctgc tatctgaata catcgcgcag ctaccagagt
     8641 aaatgagcaa atgaataaat gagtagatga attttagcgg ctaaaggagg cggcatggaa
     8701 aatcaagaac aaccaggcac cgacgccgtg gaatgcccca tgtgtggagg aacgggcggt
     8761 tggccaggcg taagcggctg ggttgcctgc cggccctgca atggcactgg aacccccaag
     8821 cccgaggaat cggcgtgagc ggtcgcaaac catccggccc ggtacaaatc ggcgcggcgc
     8881 tgggtgatga cctggtggag aagttgaagg ccgcgcaggc cgcccagcgg caacgcatcg
     8941 aggcagaagc acgccccggt gaatcgtggc aagcggccgc tgatcgaatc cgcaaagaat
     9001 cccggcaacc gccggcagcc ggtgcgccgt cgattaggaa gccgcccaag ggcgacgagc
     9061 aaccagattt tttcgttccg atgctctatg acgtgggcac ccgcgatagt cgcagcatca
     9121 tggacgtggc cgttttccgt ctgtcgaagc gtgaccgacg agctggcgag gtgatccgct
     9181 acgagcttcc agacgggcac gtagaggttt ccgcagggcc ggccggcatg gccagtgtgt
     9241 gggattacga cctggtactg atggcggttt cccatctaac cgaatccatg aaccgatacc
     9301 gggaagggaa gggagacaag cccggccgcg tgttccgtcc acacgttgcg gacgtactca
     9361 agttctgccg gcgagccgat ggcggaaagc agaaagacga cctggtagaa acctgcattc
     9421 ggttaaacac cacgcacgtt gccatgcagc gtacgaagaa ggccaagaac ggccgcctgg
     9481 tgacggtatc cgagggtgaa gccttgatta gccgctacaa gatcgtaaag agcgaaaccg
     9541 ggcggccgga gtacatcgag atcgagctag ctgattggat gtaccgcgag atcacagaag
     9601 gcaagaaccc ggacgtgctg acggttcacc ccgattactt tttgatcgat cccggcatcg
     9661 gccgttttct ctaccgcctg gcacgccgcg ccgcaggcaa ggcagaagcc agatggttgt
     9721 tcaagacgat ctacgaacgc agtggcagcg ccggagagtt caagaagttc tgtttcaccg
     9781 tgcgcaagct gatcgggtca aatgacctgc cggagtacga tttgaaggag gaggcggggc
     9841 aggctggccc gatcctagtc atgcgctacc gcaacctgat cgagggcgaa gcatccgccg
     9901 gttcctaatg tacggagcag atgctagggc aaattgccct agcaggggaa aaaggtcgaa
     9961 aaggtgtctt tcctgtggat agcacgtaca ttgggaaccc aaagccgtac attgggaacc
    10021 ggaacccgta cattgggaac ccaaagccgt acattgggaa ccggtcacac atgtaagtga
    10081 ctgatataaa agagaaaaaa ggcgattttt ccgcctaaaa ctctttaaaa cttattaaaa
    10141 ctcttaaaac ccgcctggcc tgtgcataac tgtctggcca gcgcacagcc gaagagctgc
    10201 aaaaagcgcc tacccttcgg tcgctgcgct ccctacgccc cgccgcttcg cgtcggccta
    10261 tcgcggccgc tggccgctca aaaatggctg gcctacggcc aggcaatcta ccagggcgcg
    10321 gacaagccgc gccgtcgcca ctcgaccgcc ggcgcccaca tcaaggcacc ctgcctcgcg
    10381 cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct
    10441 tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc
    10501 gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta tactggctta
    10561 actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc
    10621 acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg ctcactgact
    10681 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
    10741 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
    10801 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
    10861 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
    10921 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
    10981 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
    11041 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
    11101 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
    11161 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
    11221 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
    11281 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
    11341 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
    11401 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
    11461 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gcattctagg tactaaaaca
    11521 attcatccag taaaatataa tattttattt tctcccaatc aggcttgatc cccagtaagt
    11581 caaaaaatag ctcgacatac tgttcttccc cgatatcctc cctgatcgac cggacgcaga
    11641 aggcaatgtc ataccacttg tccgccctgc cgcttctccc aagatcaata aagccactta
    11701 ctttgccatc tttcacaaag atgttgctgt ctcccaggtc gccgtgggaa aagacaagtt
    11761 cctcttcggg cttttccgtc tttaaaaaat catacagctc gcgcggatct ttaaatggag
    11821 tgtcttcttc ccagttttcg caatccacat cggccagatc gttattcagt aagtaatcca
    11881 attcggctaa gcggctgtct aagctattcg tatagggaca atccgatatg tcgatggagt
    11941 gaaagagcct gatgcactcc gcatacagct cgataatctt ttcagggctt tgttcatctt
    12001 catactcttc cgagcaaagg acgccatcgg cctcactcat gagcagattg ctccagccat
    12061 catgccgttc aaagtgcagg acctttggaa caggcagctt tccttccagc catagcatca
    12121 tgtccttttc ccgttccaca tcataggtgg tccctttata ccggctgtcc gtcattttta
    12181 aatataggtt ttcattttct cccaccagct tatatacctt agcaggagac attccttccg
    12241 tatcttttac gcagcggtat ttttcgatca gttttttcaa ttccggtgat attctcattt
    12301 tagccattta ttatttcctt cctcttttct acagtattta aagatacccc aagaagctaa
    12361 ttataacaag acgaactcca attcactgtt ccttgcattc taaaacctta aataccagaa
    12421 aacagctttt tcaaagttgt tttcaaagtt ggcgtataac atagtatcga cggagccgat
    12481 tttgaaaccg cggtgatcac aggcagcaac gctctgtcat cgttacaatc aacatgctac
    12541 cctccgcgag atcatccgtg tttcaaaccc ggcagcttag ttgccgttct tccgaatagc
    12601 atcggtaaca tgagcaaagt ctgccgcctt acaacggctc tcccgctgac gccgtcccgg
    12661 actgatgggc tgcctgtatc gagtggtgat tttgtgccga gctgccggtc ggggagctgt
    12721 tggctggctg gtggcaggat atattgtggt gtaaacaaat tgacgcttag acaacttaat
    12781 aacacattgc ggacgttttt aatgtactga attaacgccg aattaattcg gggatagccc
    12841 tttggtcttc tgagactgta tctttgatat tcttggagta gacgagagtg tcgtgctcca
    12901 ccatgttggc aagctgctct agccaatacg caaaccgcct ctccccgcgc gttggccgat
    12961 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc
    13021 aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc
    13081 tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacat
    13141 gattacg
//
