LOCUS       sequence-476612-       12362 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian lentiviral vector for expressing the mCherry-tagged 
            Pumilio homology domain fused to the light-inducible oligomerization
            domain CRY2..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12362)
  AUTHORS   Xie Y, Shu T, Liu T, Spindler MC, Mahamid J, Hocky GM, Gresham D, 
            Holt LJ
  TITLE     Polysome collapse and RNA condensation fluidize the cytoplasm.
  JOURNAL   Mol Cell. 2024 Jul 25;84(14):2698-2716.e9. doi: 
            10.1016/j.molcel.2024.06.024.
  PUBMED    39059370
REFERENCE   2  (bases 1 to 12362)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2024.06"; journalName: "Mol Cell"; date: 
            "2024-07-25- 25"; volume: "84"; issue: "14"; pages: "2698-2716.e9"
COMMENT     Sequence Label: pHR-PUM.HD-mCherry-Cry2WT
FEATURES             Location/Qualifiers
     source          1..12362
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    43..160
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        313..720
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             2574..4067
                     /codon_start=1
                     /product="N-terminal photolyase homology region of 
                     Arabidopsis thaliana cryptochrome 2"
                     /label=CRY2PHR
                     /note="binds to CIB1 upon exposure to blue light (Kennedy 
                     et al., 2010)"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTREAQIMIGAA"
     misc_feature    4219..4807
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4272..4292)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4690..4701)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4810..4826)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4811..4827)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4917..5150
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(5175..5193)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5176..5193)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5483..5502)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(5716..5735)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5835..5857
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5895..5913)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5981..6085
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6086..6154,6155..6946)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6304..6323)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7117..7705
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7606..7625
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7859..7876
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(7946..7966)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7951..8280
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8131..8266
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          9414..9479
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             9609..9629
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    10054..10188
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10091..10110)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10145..10164
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             10356..10989
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    11036..11161
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11658..11891
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             12076..12120
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 cggatctcga cggtatcgcc aaatggcagt attcatccac aattttaaaa gaaaaggggg
       61 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
      121 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
      181 cagagatcca gtttggatcg ataagcttga tatcgaattc ctgcagcccc gataaaataa
      241 aagattttat ttagtctcca gaaaaagggg ggaatgaaag accccacctg taggtttggc
      301 aagctagctg cagtaacgcc attttgcaag gcatggaaaa ataccaaacc aagaatagag
      361 aagttcagat caagggcggg tacatgaaaa tagctaacgt tgggccaaac aggatatctg
      421 cggtgagcag tttcggcccc ggcccggggc caagaacaga tggtcaccgc agtttcggcc
      481 ccggcccgag gccaagaaca gatggtcccc agatatggcc caaccctcag cagtttctta
      541 agacccatca gatgtttcca ggctccccca aggacctgaa atgaccctgc gccttatttg
      601 aattaaccaa tcagcctgct tctcgcttct gttcgcgcgc ttctgcttcc cgagctctat
      661 aaaagagctc acaacccctc actcggcgcg ccagtcctcc gacagactga gtcgcccggg
      721 ggggatctgg agctctcgag aattctcacg cgatgggtgg tagtggcggc tctggtggaa
      781 ttccaagcgc tggcaggagc aggcttttgg aagattttcg aaacaaccgg taccccaatt
      841 tacaactgcg ggagattgct ggacatataa tggaattttc ccaagaccag catgggtcca
      901 gattcattca gctgaaactg gagcgtgcca caccagctga gcgccagctt gtcttcaatg
      961 aaatcctcca ggctgcctac caactcatgg tggatgtgtt tggtaattac gtcattcaga
     1021 agttctttga atttggcagt cttgaacaga agctggcttt ggcagaacgg attcgaggcc
     1081 acgtcctgtc attggcacta cagatgtatg gctgccgtgt tatccagaaa gctcttgagt
     1141 ttattccttc agaccagcag gtaattaatg agatggttcg ggaactagat ggccatgtct
     1201 tgaagtgtgt gaaagatcag aatggcaatc acgtggttca gaaatgcatt gaatgtgtac
     1261 agccccagtc tttgcaattt atcatcgatg cgtttaaggg acaggtattt gccttatcca
     1321 cacatcctta tggctgccga gtgattcaga gaatcctgga gcactgtctc cctgaccaga
     1381 cactccctat tttagaggag cttcaccagc acacagagca gcttgtacag gatcaatatg
     1441 gaaattatgt aatccaacat gtactggagc acggtcgtcc tgaggataaa agcaaaattg
     1501 tagcagaaat ccgaggcaat gtacttgtat tgagtcagca caaatttgca agcaatgttg
     1561 tggagaagtg tgttactcac gcctcacgta cggagcgcgc tgtgctcatc gatgaggtgt
     1621 gcaccatgaa cgacggtccc cacagtgcct tatacaccat gatgaaggac cagtatgcca
     1681 actacgtggt ccagaagatg attgacgtgg cggagccagg ccagcggaag atcgtcatgc
     1741 ataagatccg gccccacatc gcaactcttc gtaagtacac ctatggcaag cacattctgg
     1801 ccaagctgga gaagtactac atgaagaacg gtgttgactt aggcggagct accatggtgt
     1861 ctaaaggcga ggaggataac atggctataa tcaaggaatt tatgaggttc aaggtgcata
     1921 tggagggatc tgtgaacggt cacgagttcg aaatcgaggg cgaaggcgag gggcgaccct
     1981 atgaagggac acagacggcc aaactgaagg tgaccaaggg tggccccctg cctttcgcct
     2041 gggacatcct gtcaccccag tttatgtacg gctctaaggc ttacgttaag caccctgccg
     2101 atattccgga ctacttgaaa ctgagcttcc ctgaagggtt caagtgggaa cgggtaatga
     2161 attttgagga tggcggtgta gtcacagtta cccaggacag ctctcttcag gacggagaat
     2221 ttatctataa ggttaaactt cggggcacta acttcccatc agacggcccc gtgatgcaga
     2281 agaaaactat gggctgggaa gccagctcag aacgcatgta tcccgaggac ggagcactga
     2341 agggagaaat caagcagcga ctcaagctga aggatggggg acattatgat gctgaggtca
     2401 aaaccaccta taaggccaag aaacccgttc agcttcctgg ggcatacaac gtgaatatca
     2461 aactggatat tacatcccat aacgaagact ataccatcgt ggaacagtac gagcgggccg
     2521 agggcagaca tagcacaggg ggcatggatg aattgtacaa ggcggccacg cgtatgaaga
     2581 tggacaaaaa gactatagtt tggtttagaa gagacctaag gattgaggat aatcctgcat
     2641 tagcagcagc tgctcacgaa ggatctgttt ttcctgtctt catttggtgt cctgaagaag
     2701 aaggacagtt ttatcctgga agagcttcaa gatggtggat gaaacaatca cttgctcact
     2761 tatctcaatc cttgaaggct cttggatctg acctcacttt aatcaaaacc cacaacacga
     2821 tttcagcgat cttggattgt atccgcgtta ccggtgctac aaaagtcgtc tttaaccacc
     2881 tctatgatcc tgtttcgtta gttcgggacc ataccgtaaa ggagaagctg gtggaacgtg
     2941 ggatctctgt gcaaagctac aatggagatc tattgtatga accgtgggag atatactgcg
     3001 aaaagggcaa accttttacg agtttcaatt cttactggaa gaaatgctta gatatgtcga
     3061 ttgaatccgt tatgcttcct cctccttggc ggttgatgcc aataactgca gcggctgaag
     3121 cgatttgggc gtgttcgatt gaagaactag ggctggagaa tgaggccgag aaaccgagca
     3181 atgcgttgtt aactagagct tggtctccag gatggagcaa tgctgataag ttactaaatg
     3241 agttcatcga gaagcagttg atagattatg caaagaacag caagaaagtt gttgggaatt
     3301 ctacttcact actttctccg tatctccatt tcggggaaat aagcgtcaga cacgttttcc
     3361 agtgtgcccg gatgaaacaa attatatggg caagagataa gaacagtgaa ggagaagaaa
     3421 gtgcagatct ttttcttagg ggaatcggtt taagagagta ttctcggtat atatgtttca
     3481 acttcccgtt tactcacgag caatcgttgt tgagtcatct tcggtttttc ccttgggatg
     3541 ctgatgttga taagttcaag gcctggagac aaggcaggac cggttatccg ttggtggatg
     3601 ccggaatgag agagctttgg gctaccggat ggatgcataa cagaataaga gtgattgttt
     3661 caagctttgc tgtgaagttt cttctccttc catggaaatg gggaatgaag tatttctggg
     3721 atacactttt ggatgctgat ttggaatgtg acatccttgg ctggcagtat atctctggga
     3781 gtatccccga tggccacgag cttgatcgct tggacaatcc cgcgttacaa ggcgccaaat
     3841 atgacccaga aggtgagtac ataaggcaat ggcttcccga gcttgcgaga ttgccaactg
     3901 aatggatcca tcatccatgg gacgctcctt taaccgtact caaagcttct ggtgtggaac
     3961 tcggaacaaa ctatgcgaaa cccattgtag acatcgacac agctcgtgag ctactagcta
     4021 aagctatttc aagaacccgt gaagcacaga tcatgatcgg agcagcagcc cgggatccac
     4081 cggtcgccac cggttcaggg agcggatccg gctcagggag tgcggccgca actcccacct
     4141 gcaacatgcg tgactgactg aggccgcgac tctagagtcg acctgcaggc atgcaagctt
     4201 gatatcaagc ttatcgataa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     4261 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     4321 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     4381 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     4441 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     4501 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     4561 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg
     4621 tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     4681 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     4741 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     4801 tccccgcatc gataccgtcg acctcgaggg aattaattcg agctcggtac ctttaagacc
     4861 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga
     4921 agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg
     4981 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     5041 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     5101 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gcatctagaa
     5161 ttaattccgt gtattctata gtgtcaccta aatcgtatgt gtatgataca taaggttatg
     5221 tattaattgt agccgcgttc taacgacaat atgtacaagc ctaattgtgt agcatctggc
     5281 ttactgaagc agaccctatc atctctctcg taaactgccg tcagagtcgg tttggttgga
     5341 cgaaccttct gagtttctgg taacgccgtc ccgcacccgg aaatggtcag cgaaccaatc
     5401 agcagggtca tcgctagcca gatcctctac gccggacgca tcgtggccgg catcaccggc
     5461 gccacaggtg cggttgctgg cgcctatatc gccgacatca ccgatgggga agatcgggct
     5521 cgccacttcg ggctcatgag cgcttgtttc ggcgtgggta tggtggcagg ccccgtggcc
     5581 gggggactgt tgggcgccat ctccttgcat gcaccattcc ttgcggcggc ggtgctcaac
     5641 ggcctcaacc tactactggg ctgcttccta atgcaggagt cgcataaggg agagcgtcga
     5701 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc
     5761 gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca
     5821 agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg
     5881 cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat
     5941 ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     6001 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     6061 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     6121 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     6181 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     6241 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     6301 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     6361 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     6421 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     6481 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     6541 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     6601 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     6661 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     6721 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     6781 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     6841 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     6901 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     6961 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     7021 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     7081 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     7141 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     7201 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     7261 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     7321 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     7381 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     7441 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     7501 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     7561 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     7621 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     7681 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     7741 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     7801 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     7861 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg
     7921 ttggccgatt cattaatgca gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca
     7981 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt
     8041 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     8101 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     8161 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
     8221 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa
     8281 aagcttggac acaagacagg cttgcgagat atgtttgaga ataccacttt atcccgcgtc
     8341 agggagaggc agtgcgtaaa aagacgcgga ctcatgtgaa atactggttt ttagtgcgcc
     8401 agatctctat aatctcgcgc aacctatttt cccctcgaac actttttaag ccgtagataa
     8461 acaggctggg acacttcaca tgagcgaaaa atacatcgtc acctgggaca tgttgcagat
     8521 ccatgcacgt aaactcgcaa gccgactgat gccttctgaa caatggaaag gcattattgc
     8581 cgtaagccgt ggcggtctgt accgggtgcg ttactggcgc gtgaactggg tattcgtcat
     8641 gtcgataccg tttgtatttc cagctacgat cacgacaacc agcgcgagct taaagtgctg
     8701 aaacgcgcag aaggcgatgg cgaaggcttc atcgttattg atgacctggt ggataccggt
     8761 ggtactgcgg ttgcgattcg tgaaatgtat ccaaaagcgc actttgtcac catcttcgca
     8821 aaaccggctg gtcgtccgct ggttgatgac tatgttgttg atatcccgca agatacctgg
     8881 attgaacagc cgtgggatat gggcgtcgta ttcgtcccgc caatctccgg tcgctaatct
     8941 tttcaacgcc tggcactgcc gggcgttgtt ctttttaact tcaggcgggt tacaatagtt
     9001 tccagtaagt attctggagg ctgcatccat gacacaggca aacctgagcg aaaccctgtt
     9061 caaaccccgc tttaaacatc ctgaaacctc gacgctagtc cgccgcttta atcacggcgc
     9121 acaaccgcct gtgcagtcgg cccttgatgg taaaaccatc cctcactggt atcgcatgat
     9181 taaccgtctg atgtggatct ggcgcggcat tgacccacgc gaaatcctcg acgtccaggc
     9241 acgtattgtg atgagcgatg ccgaacgtac cgacgatgat ttatacgata cggtgattgg
     9301 ctaccgtggc ggcaactgga tttatgagtg ggccccggat ctttgtgaag gaaccttact
     9361 tctgtggtgt gacataattg gacaaactac ctacagagat ttaaagctct aaggtaaata
     9421 taaaattttt aagtgtataa tgtgttaaac tactgattct aattgtttgt gtattttaga
     9481 ttccaaccta tggaactgat gaatgggagc agtggtggaa tgcctttaat gaggaaaacc
     9541 tgttttgctc agaagaaatg ccatctagtg atgatgaggc tactgctgac tctcaacatt
     9601 ctactcctcc aaaaaagaag agaaaggtag aagaccccaa ggactttcct tcagaattgc
     9661 taagtttttt gagtcatgct gtgtttagta atagaactct tgcttgcttt gctatttaca
     9721 ccacaaagga aaaagctgca ctgctataca agaaaattat ggaaaaatat tctgtaacct
     9781 ttataagtag gcataacagt tataatcata acatactgtt ttttcttact ccacacaggc
     9841 atagagtgtc tgctattaat aactatgctc aaaaattgtg tacctttagc tttttaattt
     9901 gtaaaggggt taataaggaa tatttgatgt atagtgcctt gactagagat cataatcagc
     9961 cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac
    10021 ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt
    10081 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
    10141 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatcaa ctggataact
    10201 caagctaacc aaaatcatcc caaacttccc accccatacc ctattaccac tgccaattac
    10261 ctgtggtttc atttactcta aacctgtgat tcctctgaat tattttcatt ttaaagaaat
    10321 tgtatttgtt aaatatgtac tacaaactta gtagttggaa gggctaattc actcccaaag
    10381 aagacaagat atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattagca
    10441 gaactacaca ccagggccag gggtcagata tccactgacc tttggatggt gctacaagct
    10501 agtaccagtt gagccagata aggtagaaga ggccaataaa ggagagaaca ccagcttgtt
    10561 acaccctgtg agcctgcatg ggatggatga cccggagaga gaagtgttag agtggaggtt
    10621 tgacagccgc ctagcatttc atcacgtggc ccgagagctg catccggagt acttcaagaa
    10681 ctgctgatat cgagcttgct acaagggact ttccgctggg gactttccag ggaggcgtgg
    10741 cctgggcggg actggggagt ggcgagccct cagatcctgc atataagcag ctgctttttg
    10801 cctgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
    10861 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
    10921 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
    10981 tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc
    11041 tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt
    11101 gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt
    11161 cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg
    11221 aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc
    11281 agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca
    11341 accatccctt cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct
    11401 ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag acaagataga
    11461 ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccggccgct gatcttcaga
    11521 cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta
    11581 aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa
    11641 aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact
    11701 atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg
    11761 cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca
    11821 gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat
    11881 caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct
    11941 tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg
    12001 gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg
    12061 caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg
    12121 tggaattggt ttaacataac aaattggctg tggtatataa aattattcat aatgatagta
    12181 ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg
    12241 cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg acccgacagg
    12301 cccgaaggaa tagaagaaga aggtggagag agagacagag acagatccat tcgattagtg
    12361 aa
//
