LOCUS       sequence-476613-        9290 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian lentiviral vector for expressing the mScarlet-tagged 
            50nm-GEMs, encoded by the encapsulin protein from Quasibacillus 
            thermotolerans and fused to a PopZ tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9290)
  AUTHORS   Xie Y, Shu T, Liu T, Spindler MC, Mahamid J, Hocky GM, Gresham D, 
            Holt LJ
  TITLE     Polysome collapse and RNA condensation fluidize the cytoplasm.
  JOURNAL   Mol Cell. 2024 Jul 25;84(14):2698-2716.e9. doi: 
            10.1016/j.molcel.2024.06.024.
  PUBMED    39059370
REFERENCE   2  (bases 1 to 9290)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2024.06"; journalName: "Mol Cell"; date: 
            "2024-07-25- 25"; volume: "84"; issue: "14"; pages: "2698-2716.e9"
COMMENT     Sequence Label: pUBC-popTag-QtE-mScarlet
FEATURES             Location/Qualifiers
     source          1..9290
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        15..1226
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     1063..1082
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     misc_feature    3085..3673
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3138..3158)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3556..3567)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3880..4513
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(4641..4657)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4641..4657)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4654..4676)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4665..4681
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4689..4695,4696..4713,4714..4719))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4734..4755
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4872..4889)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5043..5631)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5123..5142)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5802..6593,6594..6662))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6425..6444
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6663..6767)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    6815..6949
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6852..6871)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6906..6925
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             7117..7750
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    7797..7922
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8419..8652
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8837..8881
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9144..9259
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
ORIGIN
        1 gtttatttaa ttaaggcctc cgcgccgggt tttggcgcct cccgcgggcg cccccctcct
       61 cacggcgagc gctgccacgt cagacgaagg gcgcagcgag cgtcctgatc cttccgcccg
      121 gacgctcagg acagcggccc gctgctcata agactcggcc ttagaacccc agtatcagca
      181 gaaggacatt ttaggacggg acttgggtga ctctagggca ctggttttct ttccagagag
      241 cggaacaggc gaggaaaagt agtcccttct cggcgattct gcggagggat ctccgtgggg
      301 cggtgaacgc cgatgattat ataaggacgc gccgggtgtg gcacagctag ttccgtcgca
      361 gccgggattt gggtcgcggt tcttgtttgt ggatcgctgt gatcgtcact tggtgagtag
      421 cgggctgctg ggctggccgg ggctttcgtg gccgccgggc cgctcggtgg gacggaagcg
      481 tgtggagaga ccgccaaggg ctgtagtctg ggtccgcgag caaggttgcc ctgaactggg
      541 ggttgggggg agcgcagcaa aatggcggct gttcccgagt cttgaatgga agacgcttgt
      601 gaggcgggct gtgaggtcgt tgaaacaagg tggggggcat ggtgggcggc aagaacccaa
      661 ggtcttgagg ccttcgctaa tgcgggaaag ctcttattcg ggtgagatgg gctggggcac
      721 catctgggga ccctgacgtg aagtttgtca ctgactggag aactcggttt gtcgtctgtt
      781 gcgggggcgg cagttatggc ggtgccgttg ggcagtgcac ccgtaccttt gggagcgcgc
      841 gccctcgtcg tgtcgtgacg tcacccgttc tgttggctta taatgcaggg tggggccacc
      901 tgccggtagg tgtgcggtag gcttttctcc gtcgcaggac gcagggttcg ggcctagggt
      961 aggctctcct gaatcgacag gcgccggacc tctggtgagg ggagggataa gtgaggcgtc
     1021 agtttctttg gtcggtttta tgtacctatc ttcttaagta gctgaagctc cggttttgaa
     1081 ctatgcgctc ggggttggcg agtgtgtttt gtgaagtttt ttaggcacct tttgaaatgt
     1141 aatcatttgg gtcaatatgt aattttcagt gttagactag taaattgtcc gctaaattct
     1201 ggccgttttt ggcttttttg ttagacgaat tcgccaccat gtccgaggtc gcagagcagc
     1261 tggttggcgt ctctgccgcc tctgcggctg caagcgcctt tggctcactg tcaagcgcct
     1321 tattaatgcc caaagacggc agaacactgg aggacgtagt gagagagctg ctgcggccac
     1381 tgttgaagga gtggctagac cagaacctcc ctcgcattgt agagaccaag gttgaagagg
     1441 aagtgcagcg gatcagtaga ggtcggggcg catctagaat gaataagtct cagctgtacc
     1501 cagacagtcc cctcaccgac caagatttta atcagttgga tcagacagtt atcgaagcag
     1561 cacggcgcca attggtcggc cgacgattta tcgaactgta cggacccctc ggacgcggga
     1621 tgcaaagcgt gtttaatgac attttcatgg aaagtcacga ggctaagatg gattttcaag
     1681 ggtccttcga taccgaagtg gaatcttcaa gacgcgtcaa ttacacaatc cctatgctgt
     1741 acaaggattt tgttctgtat tggcgggacc ttgagcaatc taaagcgctg gacatcccca
     1801 tagatttcag tgtagctgct aatgccgcta gggatgtggc ctttcttgag gaccaaatga
     1861 tctttcacgg ctcaaaggag ttcgacattc ctgggttaat gaatgtcaag gggcggctca
     1921 cacacctcat cggaaactgg tacgaatccg gcaatgcttt ccaagacatc gtcgaagcaa
     1981 ggaacaagtt gttagagatg aatcataacg ggccgtacgc cctggttttg tcaccagaac
     2041 tttatagcct gctccaccgg gtacacaagg atactaacgt tctcgaaatt gagcatgtca
     2101 gggaactgat aaccgccggt gtattccaaa gccccgtact gaagggaaag tccggagtca
     2161 ttgtgaatac gggacggaat aacctcgacc tcgcaataag cgaggacttc gaaaccgctt
     2221 atttgggaga agagggcatg aatcacccat tcagggtcta tgaaaccgtg gtcttgagaa
     2281 taaagaggcc agccgcgatc tgcaccctca ttgaccctga ggagtggtca gggtcaggtt
     2341 ctggctcagg tagcgggagt gggcggccgc tgatggttag taaaggtgaa gctgttataa
     2401 aagaattcat gaggtttaaa gttcatatgg aaggttcaat gaatggtcat gaatttgaaa
     2461 ttgaaggtga aggtgaaggt agaccatatg aaggtacaca aactgctaaa ttgaaagtta
     2521 ctaaaggtgg tccattacca ttttcttggg atattttgtc tccacaattc atgtatggtt
     2581 ctagagcttt tattaagcat ccagctgata ttccagatta ttataaacaa tcttttcctg
     2641 aaggttttaa atgggaaaga gttatgaatt tcgaagatgg tggtgctgtt actgttactc
     2701 aagatacttc tttggaagat ggtactttaa tctataaagt taaattgaga ggtactaatt
     2761 ttccaccaga tggtccagtt atgcaaaaga aaactatggg ttgggaagca tctactgaaa
     2821 gattgtatcc agaagatggt gttttgaaag gagacattaa gatggctttg agattgaaag
     2881 atggtggtcg gtacctggcc gacttcaaga ccacctataa agctaaaaaa ccagttcaaa
     2941 tgccaggtgc atataatgtt gatagaaagt tagatataac gtcgcataac gaggactata
     3001 ctgtagttga acaatatgaa cgtagtgaag gtagacatag taccggagga atggatgaat
     3061 tgtataaata aacgcgtctg gaacaatcaa cctctggatt acaaaatttg tgaaagattg
     3121 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     3181 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     3241 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     3301 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     3361 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     3421 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag
     3481 ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     3541 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     3601 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     3661 gccgcctccc cgcctggaat taattctgca gtcgagacct agaaaaacat ggagcaatca
     3721 caagtagcaa tacagcagct accaatgctg attgtgcctg gctagaagca caagaggagg
     3781 aggaggtggg ttttccagtc acacctcagg tacctttaag accaatgact tacaaggcag
     3841 ctgtagatct tagccacttt ttaaaagaaa agaggggact ggaagggcta attcactccc
     3901 aacgaagaca agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt
     3961 agcagaacta cacaccaggg ccaggggtca gatatccact gacctttgga tggtgctaca
     4021 agctagtacc agttgagcca gataaggtag aagaggccaa taaaggagag aacaccagct
     4081 tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagtg ttagagtgga
     4141 ggtttgacag ccgcctagca tttcatcacg tggcccgaga gctgcatccg gagtacttca
     4201 agaactgctg atatcgagct tgctacaagg gactttccgc tggggacttt ccagggaggc
     4261 gtggcctggg cgggactggg gagtggcgag ccctcagatc ctgcatataa gcagctgctt
     4321 tttgcctgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     4381 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     4441 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     4501 aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag
     4561 aaatgaatat cagagagtga gaggccttga cattgctagc gtttaccgtc gacctctagc
     4621 tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     4681 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     4741 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     4801 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     4861 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     4921 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     4981 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     5041 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     5101 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     5161 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     5221 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     5281 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     5341 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     5401 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     5461 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     5521 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     5581 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     5641 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     5701 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     5761 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     5821 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     5881 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     5941 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     6001 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     6061 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     6121 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     6181 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     6241 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     6301 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     6361 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     6421 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     6481 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     6541 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     6601 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     6661 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     6721 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     6781 aaagtgccac ctgacgtcga cggatcggga gatcaacttg tttattgcag cttataatgg
     6841 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     6901 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatca actggataac
     6961 tcaagctaac caaaatcatc ccaaacttcc caccccatac cctattacca ctgccaatta
     7021 cctgtggttt catttactct aaacctgtga ttcctctgaa ttattttcat tttaaagaaa
     7081 ttgtatttgt taaatatgta ctacaaactt agtagttgga agggctaatt cactcccaaa
     7141 gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc cctgattagc
     7201 agaactacac accagggcca ggggtcagat atccactgac ctttggatgg tgctacaagc
     7261 tagtaccagt tgagccagat aaggtagaag aggccaataa aggagagaac accagcttgt
     7321 tacaccctgt gagcctgcat gggatggatg acccggagag agaagtgtta gagtggaggt
     7381 ttgacagccg cctagcattt catcacgtgg cccgagagct gcatccggag tacttcaaga
     7441 actgctgata tcgagcttgc tacaagggac tttccgctgg ggactttcca gggaggcgtg
     7501 gcctgggcgg gactggggag tggcgagccc tcagatcctg catataagca gctgcttttt
     7561 gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     7621 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     7681 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     7741 atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct
     7801 ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg
     7861 tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg
     7921 tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg
     7981 gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg
     8041 cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac
     8101 aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc
     8161 tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag
     8221 aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccggccgc tgatcttcag
     8281 acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt
     8341 aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga
     8401 aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac
     8461 tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt
     8521 gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac
     8581 agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga
     8641 tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc
     8701 ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat
     8761 ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc
     8821 gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt
     8881 gtggaattgg tttaacataa caaattggct gtggtatata aaattattca taatgatagt
     8941 aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag
     9001 gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag
     9061 gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt
     9121 gaacggatct cgacggtatc gcctttaaaa gaaaaggggg gattgggggg tacagtgcag
     9181 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa
     9241 ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca
//
