LOCUS       sequence-476626-       11796 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses GAL4 under control of the atp6v0cb promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11796)
  AUTHORS   Moyer AJ, Barcus A, Capps MES, Chrabasz JA, Lalonde RL, Mosimann C, 
            Thyme SB
  TITLE     Genetic context of transgene insertion can influence 
            neurodevelopment in zebrafish.
  JOURNAL   bioRxiv [Preprint]. 2025 Mar 5:2025.02.28.640904. doi: 
            10.1101/2025.02.28.640904.
  PUBMED    40093151
REFERENCE   2  (bases 1 to 11796)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2025 Mar 5:2025.02.28.640904. doi: 
            10.1101/2025.02.28.640904."
COMMENT     Sequence Label: pTol2-atp6v0cb-KALTA4
FEATURES             Location/Qualifiers
     source          1..11796
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(171..962,963..1031))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     794..813
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1032..1136)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1162..1617)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1299..1320)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1511..1530
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1744..1766
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1758..1775
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1759..1775
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1785..1803
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    join(3656..3667,3668..3830)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     CDS             7297..7737
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     polyA_signal    7922..8056
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(join(8358..9071,9072..9074,9075..9077))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(8361..9071,9072..9074,9075..9077))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8403..8424)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     8752..8771
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     9011..9032
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     misc_feature    join(10376..10563,10564..10575)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(10609..10625)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10609..10625)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     protein_bind    10648..10717
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     primer_bind     complement(10767..10787)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(10767..10785)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(10806..10822)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10806..10822)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10819..10841)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10830..10846
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10854..10860,10861..10878,10879..10884))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10899..10920
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11037..11054)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11208..11796)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11288..11307)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
       61 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
      121 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
      181 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
      241 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
      301 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
      361 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
      421 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
      481 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
      541 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
      601 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
      661 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
      721 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
      781 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
      841 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
      901 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
      961 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     1021 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     1081 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     1141 tttccccgaa aagtgccacc taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa
     1201 atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata
     1261 aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac
     1321 tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc
     1381 cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa
     1441 atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg
     1501 cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg
     1561 tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtccc
     1621 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
     1681 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
     1741 tttcccagtc acgacgttgt aaaacgacgg ccagtgagcg cgcgtaatac gactcactat
     1801 agggcgaatt gggtaccaaa tagtaggaat tacccacctg tacaagtgct gaaaacttgg
     1861 atgaataagc ccgtttgcca ttttctactg ctattttaaa tcttttctgt atttgtctgc
     1921 atttgtcttt acccttgaat aaatgtttta gttgtttttt ttcaattatg actgtgttta
     1981 acagacatta ttacaataat atgtaatagt agtacacact attattatgt aatagtactt
     2041 gttgactgta tttgagaact ggaccgagtg agtgttacgt cacccattca aaatgactta
     2101 cttctggctc caacaaaatg aagttaattc agttgccatt tttcactgta tggacatcgc
     2161 cgtgttggag ctagacgttg ccaataagca agaaagagcc gagatgcgtc gagtctgagt
     2221 caccgttcct atggcaaccc ctctaaccaa tcagaagtaa gcttgttgga agtccacagc
     2281 ctaccacttg aaagcgggct gcacaaaatc tgtcaaacgt tttgaacgtt ggatgtgaga
     2341 gcacatactt ttattaaggc atctggttgg tcagtttata atatcaacaa cttgggctac
     2401 agaaaagaaa agttattaca gaaattatga ttaacaagta catgttaaat aaagatttta
     2461 atatgaatgc caccactgga gcattcatgc catttggagc ttcttcctgt ttggatcact
     2521 agaaggagga ggtcactcat tacagttctc atatacagtc gttggttggt tggttggttg
     2581 gtagattgat tgattgattg attgattgat tgattgattg attgattgat tgattgattg
     2641 attgattgat tgattgattg gtagtcaaaa taagaaataa tttccacaga ttcattacag
     2701 aaatgattaa atgcatacat aaaaaactgg gggggggggg ggatacaaca acacacttaa
     2761 gtcacatttg cctacgtaaa gaaaagtaaa gaaaatcaat agctatattt tacatctcct
     2821 ttttttgctg tcttttaatt agccttgttt tgctgttatc ttgattgaaa ctgtaacttc
     2881 ttcacctgct tcttttcttt gtaggttttg ccagcccaga aacaggcatg gctgtgcaag
     2941 gccagagcac catgcacaat gcgctgcatg tcttcatgaa cggctcaatg tcctcagtcc
     3001 agggctcagc caacgacccc atcttcctcc ttcaccatgc tttcattgac aggtaacaaa
     3061 cacgtcatga cattagactg cacagttttt gacaaagttc atacaatctg ttgtttatag
     3121 ctgctacaat tagtgaagtt tgtgaatgta cttggatgag cagcgaaaga tcaattgaga
     3181 tcaattgtta gagtttggtt gccctgcaga gcaaagaaca aaaaataatc tggtggcttt
     3241 actgcgtgag gttattattg gtggaataga aacacaaaac ataattgcat ttatttgttt
     3301 aattttttat cttatcttaa ctttcatctt gcatatttgt ttcttacatc atttctagca
     3361 tctttgagcg ctggctaaga actcatcagc ctccccggtc catctaccca cgtaccaatg
     3421 caccaattgg ccacaatgac ggctactaca tggtgccatt ccttcctctt tataggaatg
     3481 gagactacct cctgtccaac aatgctcttg gatacgagta cgcctacctg ttggacccag
     3541 gtcattgcac aacaccagaa atgccctctg atctgcaaaa gacgtgaata atctgttcag
     3601 acacccatat gcactctgtt ccacacaggt cagaggtttg tccaggagtt cttgacagag
     3661 gtgtaaaaag tactcaaaaa ttttactcaa gtgaaagtac aagtacttag ggaaaatttt
     3721 actcaattaa aagtaaaagt atctggctag aatcttactt gagtaaaagt aaaaaagtac
     3781 tccattaaaa ttgtacttga gtattaagga agtaaaagta aaagcaagaa agaaaactag
     3841 agattcttgt ttaagctttt aatctcaaaa aacattaaat gaaatgcata caaggtttta
     3901 tcctgcttta gaactgtttg tatttaatta tcaaactata agacagacaa tctaatgcca
     3961 gtacacgcta ctcaaagttg taaaacctca gatttaactt cagtagaagc tgattctcaa
     4021 aattgttagt gtcaagccta gctcttttgg ggctgaaaag caatcctgca gtgctgaaaa
     4081 gcctctcaca ggcagccgat gcgggaagag gtgtattagt cttgatagag aggctgcaaa
     4141 tagcaggaaa cgtgagcaga gactccctgg tgtctgaaac acaggccaga tgggccattc
     4201 gtacgcttaa tttcgtatag ggataacagg gtaatgatct aggcacattc agatggtcgg
     4261 gtcagagttt ggtgtaagca acatgtaagc atggatccat cctgccttgt atcagcggtt
     4321 caggctggag gtggtgtaat ggtgtggggg agattttctt ggcacacttt gggtccatta
     4381 gtaccaatta agcatcgtgt caacgccaca gactacccta atgttgttgc tgaccatgtc
     4441 catcccttta tgaccacagt gtacacatct cctgatggct gcttccagca ggataacgca
     4501 ccatgttata aagtacaaat catctcagac tggtttcttg aacatgacaa cgagttcact
     4561 gtactcaaat ggcctccaca gtcatcagag ctcaatccaa tagagcactt ttgggatgtg
     4621 gttgaatggg agattggcat catgaatgtg cagccgacaa atctgcagca actgtgtgat
     4681 gctatcatgt taatatggag caaaatctct gaggaatatt tccagtatct tgttaaatct
     4741 tgccacaaag gattaaagta gttctgaatg caaaatgggg cccaacccgg tactagtaag
     4801 gtgtacctaa taaagtggcc agtgagtgta tgcataaatt gattcatgtg ccaatatcta
     4861 aacatcatta aatcgagaag cgttttctac acaagcataa aatattggtc ccactttata
     4921 ttaagtggcc ttaactaata tgtacttaca taggaattaa tagtttgtta caatgcactt
     4981 gttgtgtaaa tacatgtatt tactgtgtac ttatacttga ttaaatacat gtatgtaatt
     5041 acatctataa ttaacttttg taattacatt tgtaaataca ctgttgacca tcccttacac
     5101 cttaacctac ccttaaacct acccatgcca acaaacctgt tcataaccca acctctattc
     5161 caactcaaaa gcaccagaag tgttctcaaa tacattataa acacagtaag tacattgtat
     5221 ttattttttt atgtaagtac atagtagtta gggacactta atataaagtg ggaccaaaat
     5281 attttcttgt tttaaaaaat aatttaaatt atgaatgatt taataatctg ccagtggggt
     5341 aagcaaaata atcgtatgtc aaatggaaaa actcaacttt ttttgcatgc tgtaaggaaa
     5401 aaactcactt aattttgacg tattatttct taaaacccaa ccttgtctgg aaaatgctgc
     5461 tcgatttagg gatttttaga tatttggacc agaaactaca ctaaatatct aagtaagaga
     5521 agcatttatt gcagtgtact caattgagaa aaatccctgt taatacatct cttcaatatt
     5581 aacgtcatat caaaactata gttctattac tgtaatttaa tataatacat tatttatgaa
     5641 acacaaaaca acctatggtt tcccagtact gggttgcggc tggtagggca tccactgtgt
     5701 aaaacatgct ggataagttg gcggttcatt ccactgtggc gacccctgat gaataaaggg
     5761 actaagcaga aggaaaatta atgaagacaa taaaagttga ccaagaacac aaagtaaaaa
     5821 ggctctcaga ttaatcagta aggtcagctt tacaactgta ccctgcacta aaatctgaga
     5881 aattctcaat cacacgcgcg cctgtcattt gacctttatg atgcagattt tccctttggc
     5941 taaatgagac cagactaatt gatctctcct cgtgtgtcct gaacaaccat gtctaccatg
     6001 ctgacataaa caccgcataa ttactcacaa ttatttgcat taaaaataca cttttgttta
     6061 tttgatgcat tattgactgg gaataaaata tgcattaacc aaccatctaa taaacacccc
     6121 acctggcttt atctcatgtt tattcatatt tttttgtgaa tattcttaaa ttcttgctga
     6181 catgagccgg tgggcgtggc ttgcgcttga cgtcactggt cagaatgacc ttcatctcgg
     6241 gaatcctcat ccgctgcatc acactttact gcgtggctga cataatatct cttatatgat
     6301 aaagaagcta cattataatg tgatgttgat catctgaggc ctatattaga tacgttagat
     6361 aacgtttgaa agaccaaatg agttttctta atgatatatc atcgaatgca gtgtgatagt
     6421 ataatcatgt acgcccttac aaacagtact tcatgttaaa atgctgacac tatggaaaaa
     6481 tattaaataa atgtattata catacaattc ggtattaata ttatacataa atggcgcatg
     6541 ttgatatatt ttagtgcaca cgttcttaat atatctagga aataatttat tataagttac
     6601 ataatgctta aaaccttcta atctctttaa aatatattta acacatcgat ttctcccatt
     6661 attttacatt atttatgaag gaaaacagtg ttatatgtgt atctggtgta gaatatacgt
     6721 gctgcaatga tttttagagc atgattaaat gatttaaatg aaagtaatac gaaactagta
     6781 tgttcacacc cagattacaa acgcacgatg ttttattcta agggtttaaa catcttcagt
     6841 attaaaggga aagcccgcag cggagcgcac taaactcgct acaaatgggg gagccccgaa
     6901 atcaaatacc ctctgaaata ctgttagcat tgcacgtaaa tgcttcatta tatttgtttt
     6961 acaatgttta tccatgtcat gttcgttcca tgactcgaat agaagcagta taccagcgca
     7021 tccctgtttt tctcgatctc ctcccccttt caggagtcgg atgggatttt cttcacggtg
     7081 tgtcaggtga cgttttcggc cattttgtac cgcagattca tcctacagga tccgttacga
     7141 gcagtttcca tcatttcacc ggggtttatt aaaaccattg gtccatagcg aaagctttac
     7201 cctaatagac aaatacccgt tagtctatac gccttcgctc gtgtccagca gtgtaagttc
     7261 ggtgttactg ggttttgagg tgaaattaga ggaataatga aactgctctc atccatcgag
     7321 caagcctgcg acatttgtcg gcttaagaag ctgaaatgct ccaaggaaaa gccgaaatgt
     7381 gccaaatgcc tgaagaacaa ttgggaatgt cgttactctc ccaaaaccaa gcgaagtcca
     7441 ctcacaaggg ctcatctgac cgaagtggag agcaggctag agagactgga acaactcttt
     7501 ttgctcatct tccctagaga ggaccttgac atgatcctca agatggattc tctccaggat
     7561 attaaagccc ttttgactgg cttattcgtc caggacaatg tgaacaaaga cgctgtgaca
     7621 gaccgattgg caagtgtcga gaccgatatg cctctgacac tgagacagca cagaatcagc
     7681 gctacttcct caagcgaaga gtcttctaac aagggacaga gacagctgac tgtttcgagc
     7741 aggtcgaccc cgtccccggc cgacgccctg gacgacggcg acctggacat gctgcctgct
     7801 gatgctctcg atgatttcga tctggatatg ctcccggccg acgccctgga cgactacgac
     7861 ctggacatcc tcccgggtaa ctaacaaact cgagaaagct agcaaaccat ggtacgatga
     7921 tgatccagac atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg
     7981 aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag
     8041 ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga
     8101 ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtatgg ctgattatga
     8161 tcctctagat cagatctctt gtttattgca gcttataatg gttacaaata aagcaatagc
     8221 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     8281 ctcatcaatg tatcttatca tgtctggatc tacgtaatac gactcactat agttctagag
     8341 gctcgagagg ggccgcttta cttgtacagc tcgtccatgc cgagagtgat cccggcggcg
     8401 gtcacgaact ccagcaggac catgtgatcg cgcttctcgt tggggtcttt gctcagggcg
     8461 gactgggtgc tcaggtagtg gttgtcgggc agcagcacgg ggccgtcgcc gatgggggtg
     8521 ttctgctggt agtggtcggc gagctgcacg ctgccgtcct cgatgttgtg gcggatcttg
     8581 aagttcacct tgatgccgtt cttctgcttg tcggccatga tatagacgtt gtggctgttg
     8641 tagttgtact ccagcttgtg ccccaggatg ttgccgtcct ccttgaagtc gatgcccttc
     8701 agctcgatgc ggttcaccag ggtgtcgccc tcgaacttca cctcggcgcg ggtcttgtag
     8761 ttgccgtcgt ccttgaagaa gatggtgcgc tcctggacgt agccttcggg catggcggac
     8821 ttgaagaagt cgtgctgctt catgtggtcg gggtagcggc tgaagcactg cacgccgtag
     8881 gtcagggtgg tcacgagggt gggccagggc acgggcagct tgccggtggt gcagatgaac
     8941 ttcagggtca gcttgccgta ggtggcatcg ccctcgccct cgccggacac gctgaacttg
     9001 tggccgttta cgtcgccgtc cagctcgacc aggatgggca ccaccccggt gaacagctcc
     9061 tcgcccttgc tcaccatggt cactgtctgc tttgctgttg gtctgggctc ctgggtcact
     9121 ggcttactaa tggagtcttt atgtatgagg actcttatca attgttcttc tataaaggtc
     9181 tgcagtgttt ctgttcgtcc cctacatgga cacccagagc ctcctaaata caggagccct
     9241 gataactgca caagtgctca gattccagca gggtggaaaa tgagataaag tgtgcagatg
     9301 gggaggggga cgtgaatgag agatttgagg gatgaaaagg atggatgaac gcattgaaaa
     9361 tagccccttt cacaaaataa taccagtaaa ttgtcatata attaatgacc agacctttac
     9421 ctgtaaatga tgtgatgttc atatacagaa gaatgccaac aatttacaga tgattttaca
     9481 aattcttaat tttccttttc taaatggatt tgttcacaca ttatctcttc aaagcaattg
     9541 aattttctgg aaaagattgg ctgtgtgtgt aaaaggtgct actgaggaat ttaatgtgac
     9601 atggaatgaa gcaaacagca gcctaagaca ggatggcagg aaaaatgtca catgtttaaa
     9661 ataagagtga aaccaacgac ctggacttat agagactgtg tgtccttagg caaagctctt
     9721 gtacaaacag tgcttgtgac tgtgaaattc ggggtttgcc tggattgtgt taaatgtgtg
     9781 cttggatgta tcataaaaaa gaatcggttc tctgtatttg acattttctg taatttatct
     9841 aaagttacaa ctggctatgc ctgattttta tcactattga atcaatcatt ttaaagaatg
     9901 ttaagtctgc tgatttggag agctcattta catttatttt gaatgtctct tatttaacac
     9961 aactgattta agctttagat ctaatctgat cattcaggcg cgccaacacg tgaataccac
    10021 gcgaggcctt agggataaca gggtaatacg cgtgatctgc gaagatacgg ccacgggtgc
    10081 tcttgatcct gtggctgatt ttggactgtg ctgctcgcag ctgctgatga atcacatact
    10141 tcctccattt tcttccactg attgactgtt ataatttccc taatttccag gtcaaggtgc
    10201 tgtgcattgt ggtaatagat gtgacatgac gtcacttcca aaggaccaat gaacatgtct
    10261 gaccaatttc atataatgtg aaaacgattt tcataggcag aataaataac atttaaatta
    10321 aactgggcat cagcgcaatt caattggttt ggtaatagca agggaaaata gaatgaagtg
    10381 atctccaaaa aataagtact ttttgactgt aaataaaatt gtaaggagta aaaagtactt
    10441 ttttttctaa aaaaatgtaa ttaagtaaaa gtaaaagtat tgatttttaa ttgtactcaa
    10501 gtaaagtaaa aatccccaaa aataatactt aagtacagta atcaagtaaa attactcaag
    10561 tactttacac ctctggttct tgacccccta ccttcagcaa gcccagcaga tccactagtt
    10621 ctagagctcg acgatgtagg tcacggtctc gaagccgcgg tgcgggtgcc agggcgtgcc
    10681 cttgggctcc ccgggcgcgt actccacctc acccatctgg tccatctgat gaacgggcca
    10741 ccgcggtgga gctccagctt ttgttccctt tagtgagggt taattgcgcg cttggcgtaa
    10801 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
    10861 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
    10921 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
    10981 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
    11041 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
    11101 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
    11161 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
    11221 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
    11281 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
    11341 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
    11401 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
    11461 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
    11521 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
    11581 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
    11641 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
    11701 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
    11761 aagcagcaga ttacgcgcag aaaaaaagga tctcaa
//
