LOCUS       sequence-476791-       16676 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express NanoLuc(R)-MLL1 Fusion Protein in Mammalian Cells under a 
            CMV promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16676)
  AUTHORS   Promega
  TITLE     Promega Plasmid Collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 16676)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: NanoBRET Assay Vector, NanoLuc-MLL1
FEATURES             Location/Qualifiers
     source          1..16676
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(4578..5090)
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     primer_bind     complement(5103..5122)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5104..5122)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5164..5183)
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     intron          5166..5298
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     promoter        complement(5434..5637)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(5463..5483)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        5638..6017
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    6208..6491
                     /label=cer region
                     /note="ColE1-derived recombination site that helps to 
                     maintain plasmids as monomers"
     rep_origin      6607..7195
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7096..7115
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    7434..7482
                     /note="synthetic polyadenylation signal"
     CDS             complement(7546..8340)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7657..7676)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     8267..8286
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(8372..8419)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(8434..8791)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8448..8583
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        8600..8791
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     8748..8768
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     polyA_signal    9036..9157
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9073..9092)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9127..9146
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     9194..9212
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 gtgggtttgg tggggtagtt tggtcccagg cactcagggt gatagctgtt tcggcactta
       61 ttacactcca gcagctgctt tgtagcctga tgttgccttc cacaaacgtg acagaatttg
      121 caacgacgac aacaccaatt ttccagctgg tcctccagag ggcgctcgtt ctcctctaaa
      181 caaaacttgt ggaagggctc acaacagact tggcaataca caaactctac atgcccacta
      241 ctggcacaga gaaagcaaac caccctgggt gttataggaa cagaagtcaa gattcctaag
      301 cctcccatct cccacacatt ttctgcttca caatcctcct taaagtccac tctgatcctg
      361 tggactccat ctgctggaat tttttgctta gaactattgc cattggagag agtgctgagg
      421 atgttcaaag tgcctgcatt ctcctgctta ttgaccggag gtggtttttc cttttctttt
      481 ggtttttgtt ttacagggat acttgggcgg ggagccactt ttttctgttt gctctgctct
      541 ggacctgatt ctggtggtgg aggctgcttt ttcttgggct cactaggagt ggttttggga
      601 acttcttttc ttggcggtcc tgtagtaggt ggctgaggcg ggatgaccag tgctggctgg
      661 gagacctgct tgcttgactt cctggaagct ggagtggtgg cctgtttgga ttcaggccca
      721 ggggccgaga cattcccttc ttcactcttt tcctcgacgg gctttcgtgg aggaggctca
      781 ctactgcttt tctttggggc aggatcctct cttgctgatg gggtaggttt ctgactagag
      841 tccaccacgt tcttcacaac actgctctct ttgctgtctt tcttttcact ggtcttagac
      901 tttttctctt tctttttcac agctttagct tgcttctgca ggtaggcttt ggaaggcatc
      961 cattgtagat tctgacattt tctcatcttg cagcactgct tctttatatt gcgaccacca
     1021 aacttgggct tatctaagca attagtacaa acaccacagt cctcaggcac ctggcagccg
     1081 ggacactgcc cacaccgcct cgatcgacgt cctttcttta ctggaggttc ctggggtgcc
     1141 ttgtttctag tgacaggttt aattggtttg atgggtggag caagaggttc agcatcttct
     1201 gagccagcaa ttgatgactt gtcatcattc cccatggaag acaaaatctt ttctcgttct
     1261 tcccatggta aggcactcag ggtgggcatg tcatcaggaa acacagctcg ttttcggcca
     1321 agggcaacag ctgctcttct gcagacatgt ttaatccggg gtcctcgcac agaggtctct
     1381 gatgagtcac tttcttgacc ctgtgctttg ggctggtcgg tttgtttaag actcttactc
     1441 ttctcaatct tgcagagctg agctttggcc ttttttagga ggctggcaac cctcttgtca
     1501 gtcattggaa gcttgtctgc ctgagccaac atggagccta tggaggaagt ggaatgttta
     1561 acagtgctag atgaaggagt ggaaaggcag agggttttct ccttctccag ggatggggca
     1621 gttgggccga ggtccaagtt ggttttttcc agatttcctc tccctttctt tataagtatt
     1681 ttggttttga cagctgttgt atccccaaga gtcacagaag taatatcagt cccagaatca
     1741 tgtgatgaag acttcttccg ccctgttgct tttttggcag aagatgaagt ggcaacatct
     1801 tcaccaacaa ccttctcttt ggaaacccta cccacaggat acaaagcaga actactctga
     1861 atttctgatc cctttttcct tttctctttc cttgactccc gcttattctc cttttctctc
     1921 tcccggtctc tctctctact cttgtccttc tccacgctct tgtcagcatc tcgatctttg
     1981 gacagctcct cgggggcctt gtctttattt ctccctcttt cagtctgaga gcctggggta
     2041 aaccaaggga agagaggagt aggactactt gatgaaaatg gctctgccgg agcactagtc
     2101 tgcttccttg gtctctgatt tttctctgca gattccccag actgagtcag ggaatgagaa
     2161 ggaaaagtaa aagttgggtt taaggcacta gtggcaagag gactaacaga aatgcttaac
     2221 gaggaagaga cagaagacgg gggggtgaga ggtgagagct cagaactact aagccttcca
     2281 cttcttgtcc tcatggagtg agaaggagat cttggttcag atcgaatagg actaaacact
     2341 tttcttttcc tttttctatt ggatactcct gaagaagatg ttccagcaga agttcgatta
     2401 ctaggcaagg ttacagactc aaatattcta gagtgagcct cacttggagt aaatcttgga
     2461 gctctcagaa gagggctcct tttgtgcata tcaaaccttg ttccagaatg gagtggcgaa
     2521 aacaatcggg ctgaagcagc ggtaccagat gcagaaaaac cagatgcaaa gccaacgtcc
     2581 tcgggagtta gtggaggggg tcggaaatta tcaaatattg gtttgcgaat aagaccttct
     2641 ttggcatact ttgctgagga aaagtattgt ggctctgacc tagaatgctt taaagaagtc
     2701 cacctaaatg tcggttctcg caaaatagat tttcgcttcc cttgcatagg agcagtggaa
     2761 gcaggcaaaa atggtgatgc taaggggatt gttggaggca taagccaagg tgtgtggtca
     2821 gagatactgg aggctggctg cagtggtggc ggtggagtca gcagaggtgg aggtggagac
     2881 gaggaggtct gctgctgggg ggcactttgt agagttgata attttttcgt cgttctagat
     2941 ccaaaacttc tctccgacac tgaatacctt ctgcttctcc tatcattact ctcattttct
     3001 ggggactggg aaatgggcag tggaggatga acttcagggg tatcgctccg ctcctcagga
     3061 agtacctgaa tctcctcaga agcctgagag tctgtggagg tatcaacact ggggctacta
     3121 gatcgagagg agtctgaaga catttgagag gagtgctgag aagctgcact tgatttttca
     3181 gaagatccac aggacggggc actgaatcta ctattcggtg tagactctaa tcgggcaatt
     3241 ttaattggag ggtcataatc ctcatcctct ataaaccgcc gaggggtctt aatgatccgc
     3301 gaggagatag cactgacaac aggcatgatg aactgtcgaa tatttttgac ctgtgtcttc
     3361 acctttcttc cctgcagctg agctgcttct ttttcaattt tcttttgagc cccctttttt
     3421 gccctctgta agagttgctt agcaatggtt gcatctgtcc tttttgaaga aggaataatc
     3481 ctaactggct taatccttcg agggctttgt ctgacaactg tcttatcttc ttttgtaagt
     3541 ggaggtgttc cttccttgtc tttccggact ttctggggct tttccagttc agaattaatg
     3601 aggagacccg aaggggtctt tatcctttct gttgatggag gccttcctct ccgtcgtaca
     3661 atttgtaccc ccttccttcc tatttgaagc ttccctgtct taaacttaga cttgagagga
     3721 gagagtttac ctgctcttaa ttttttaatc tttgtggctt gctgaaacgt agcagaaggt
     3781 gtccttttaa tttttttcag gctatcttct ttgtttccct ttggtaactc tgaaatgtcc
     3841 tttccatgtg ttattttgat ttttactcca gggaaggtgg gaggtcttcc tctcttcttt
     3901 tctatacttt tagaatcttt cttcttgatc ttatctccag atttggtctc tgatttattt
     3961 agaggggaaa acacagatgg atctgagagg atagctgaat ttcggtcaga gccacttcta
     4021 ggtctcccac gaggttttcg aggactagtt ttaactgaag gagaccttgt gggacttcgc
     4081 actctgactt cttcatctga gccaaaacct aagaattgct catcctctcc gctgccgcct
     4141 cccccgccgc tctccccaaa cacggcccgg aaccggcgca ggttggtgcc gatggcggcc
     4201 gagacctgca gcgccgcgtc gaagcccggg cccacccgga gcagggccgg ccctgaagag
     4261 gctgaggacg atgacgaaga cgaagacgag gcggacgacg aggaggctgc tgaggcggcg
     4321 gccgctcccc ctggaacccc agccccgctg cttcccgccg ccgccgccgc ggccgccaca
     4381 gccggggggg aggggggcgc cccggggccg ccaccgccga ccgggggccc ggggggaagc
     4441 agcagggccg ggacgcgttg ccgcggggcg ccccctaggc cccggcgccc cccgccgccg
     4501 ccacccccgg tggtcccggg tcgggcgggg aagcgccacc gacagctgtg cgccatggcg
     4561 atcgcgccgc tcgagcccgc cagaatgcgt tcgcacagcc gccagccggt cactccgttg
     4621 atggttactc ggaacagcag ggagccgtcg gggttgatca ggcgctcgtc gataattttg
     4681 ttgccgttcc acagggtccc tgttacagtg atctttttgc cgtcgaacac ggcgatgcct
     4741 tcatacggcc gtccgaaata gtcgatcatg ttcggcgtaa ccccgtcgat taccagtgtg
     4801 ccatagtgca ggatcacctt aaagtgatga tcatccacag ggtacaccac cttaaaaatt
     4861 ttttcgatct ggcccatttg gtcgccgctc agaccttcat acgggatgat gacatggatg
     4921 tcgatcttca gcccattttc accgctcagg acaatccttt ggatcggagt tacggacacc
     4981 ccgagattct gaaacaaact ggacacacct ccctgttcaa ggacttggtc caggttgtag
     5041 ccggctgtct gtcgccagtc cccaacgaaa tcttcgagtg tgaagaccat ggtgagcgct
     5101 agccctatag tgagtcgtat taatactcta gccttaagag ctgtaattga actgggagtg
     5161 gacacctgtg gagagaaagg caaagtggat gtcagtaaga ccaataggtg cctatcagaa
     5221 acgcaagagt cttctctgtc tcgacaagcc cagtttctat tggtctcctt aaacctgtct
     5281 tgtaaccttg atacttacct gcccagtgcc tcacgaccaa cttctgcagc ttaagttcga
     5341 gactgttgtg tcagaagcac tgactgcgtt agcaatttaa ctgtgataaa ctaccgcaat
     5401 aaagcttcta gtgatctgac ggttcactaa accagctctg cttatataga cctcccaccg
     5461 tacacgccta ccgcccattt gcgtcaacgg ggcggggtta ttacgacatt ttggaaagtc
     5521 ccgttgattt tggtgccaaa acaaactccc attgacgtca atggggtgga gacttggaaa
     5581 tccccgtgag tcaaaccgct atccacgccc attggtgtac tgccaaaacc gcatcaccat
     5641 ggtaatagcg atgactaata cgtagatgta ctgccaagta ggaaagtccc gtaaggtcat
     5701 gtactgggca taatgccagg cgggccattt accgtcattg acgtcaatag ggggcggact
     5761 tggcatatga tacacttgat gtactgccaa gtgggcagtt taccgtaaat actccaccca
     5821 ttgacgtcaa tggaaagtcc ctattggcgt tactatggga acatacgtca ttattgacgt
     5881 caatgggcgg gggtcgttgg gcggtcagcc aggcgggcca tttaccgtaa gttatgtaac
     5941 gcggaactcc atatatgggc tatgaactaa tgaccccgta attgattact attaataact
     6001 agtcaataat caatgccaac atggcggtca tattggacat gagccaatat aaatgtacat
     6061 attatgatat agatacaacg tatgcaatgg ccaatagcca atattgattt atgctatata
     6121 accaatgaat aatatggcta atggccaata ttgaagatct cgatcctcta cgccggacgc
     6181 atcgtggccg gagtactgcg gtttatctcg atggctacga gggcagacag taagtggatt
     6241 taccataatc ccttaattgt acgcaccgct aaaacgcgtt cagcgcgatc acggcagcag
     6301 acaggtaaaa atggcaacaa accacccgaa aaactgccgc aatcgcgcct gataaatttt
     6361 aaccgtatga atacctatgc aaccagaggg tacaggccac attaccccca cttaatccac
     6421 tgaagctgcc atttttcatg gtttcaccat cccagcgaag ggccatccag cgtgcgttcc
     6481 tgtatttccg gactataaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     6541 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     6601 atcttcttga aatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     6661 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     6721 tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca
     6781 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     6841 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     6901 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     6961 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     7021 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     7081 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     7141 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     7201 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     7261 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     7321 cgggtagaaa gccagtccgc agaaacggtg ctgaccccgg atgaatgtca gctactgggc
     7381 tatctggaca agggaaaacg caagcgcaaa gaggatcctt atcgctatcg attcacacaa
     7441 aaaaccaaca cacagatgta atgaaaataa agatatttta ttgcggccat cgtgatggca
     7501 ggttgggcgt cgcttggtcg gtcatttcga accccagagt cccgctcaga agaactcgtc
     7561 aagaaggcga tagaaggcga tgcgctgcga atcgggagcg gcgataccgt aaagcacgag
     7621 gaagcggtca gcccattcgc cgccaagctc ttcagcaata tcacgggtag ccaacgctat
     7681 gtcctgatag cggtccgcca cacccagccg gccacagtcg atgaatccag aaaagcggcc
     7741 attttccacc atgatattcg gcaagcaggc atcgccatgg gtcacgacga gatcctcgcc
     7801 gtcgggcatg cgcgccttga gcctggcgaa cagttcggct ggcgcgagcc cctgatgctc
     7861 ttcgtccaga tcatcctgat cgacaagacc ggcttccatc cgagtacgtg ctcgctcgat
     7921 gcgatgtttc gcttggtggt cgaatgggca ggtagccgga tcaagcgtat gcagccgccg
     7981 cattgcatca gccatgatgg atactttctc ggcaggagca aggtgagatg acaggagatc
     8041 ctgccccggc acttcgccca atagcagcca gtcccttccc gcttcagtga caacgtcgag
     8101 cacagctgcg caaggaacgc ccgtcgtggc cagccacgat agccgcgctg cctcgtcctg
     8161 cagttcattc agggcaccgg acaggtcggt cttgacaaaa agaaccgggc gcccctgcgc
     8221 tgacagccgg aacacggcgg catcagagca gccgattgtc tgttgtgccc agtcatagcc
     8281 gaatagcctc tccacccaag cggccggaga acctgcgtgc aatccatctt gttcaatcat
     8341 gatctgcctc ctgggtttag ttcctcacct tgtcgtatta tactatgccg atatactatg
     8401 ccgatgatta attgtcaaca gttaattaag ctttttgcaa aagcctaggc ctccaaaaaa
     8461 gcctcctcac tacttctgga atagctcaga ggccgaggcg gcctcggcct ctgcataaat
     8521 aaaaaaaatt agtcagccat ggggcggaga atgggcggaa ctgggcggag ttaggggcgg
     8581 gatgggcgga gttaggggcg ggactatggt tgctgactaa ttgagatgca tgctttgcat
     8641 acttctgcct gctggggagc ctggggactt tccacacctg gttgctgact aattgagatg
     8701 catgctttgc atacttctgc ctgctgggga gcctggggac tttccacacc ctaactgaca
     8761 cacattccac agctggttct ttccgcctca gaaggtacct aaccaagttc ctctttcaga
     8821 ggttatttca ggccatggtg ctgcgcagat ccgcgtatgc ggtgtgaaat accgcacaga
     8881 tgcgtaagga gaaaataccg catcaggaaa ttgtaagcgt taatattttg ttaaaattcg
     8941 attttaccac atttgtagag gttttacttg cttaaaaaaa cctcccacat ctccccctga
     9001 acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg
     9061 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     9121 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgctcg aagcggccgc
     9181 cccaaggggt tatgctagtt attgctcagc ggtggcagca gccaactcag cttcctttcg
     9241 ggctttgtta gcagccggat cagcttgcat gcctgcaggt cgactctaga ggatccccgg
     9301 gtaccgagcc cgaattcgtt taaacgttta ggaacttccg gcatttcttg gcgccacagt
     9361 tgcagggcag cttgttgctg gcatcctcaa tggggaactt atagtcgtaa gtgagttcct
     9421 ctcctcggta gatcttacgc atggcaaaga tgacaatgtg cttctgccca tcaatattga
     9481 tgacccgaga atagcagtta ggctcacacg agtgattgat gaagcgtgca gcatttccat
     9541 gcatggtggc atccactacc tctgagtcat caattcggaa catatagcaa ccaatgccct
     9601 tgctgtcgta atacttttcc cgcttgtcag tctggatgga gcggatgacg ttgccggcat
     9661 actcaatcac catctcacct gcatcaatgt ttctcttaca gaaaagaccc cggccatgga
     9721 tgggagacct gtagacacca actgcctcct tagaagtctt ttttaagtgc cggaagcgca
     9781 tgggcattgg cagatccatg ctagttgccc tccgagctga cttcagctgt acctcctcct
     9841 cttcttcatc attggggttg tattcaggag gctgacgatg tttagaagcc aggaagttaa
     9901 acatgtcaaa tgctgacttc ctgaggtgga cttcagccct ggctgagccg tgagggttca
     9961 aggggggttc attggcctcc tctggcttgt ggaaacggaa tttgtaattt cgacagtgct
    10021 tggcaccaga cagctgctca atgaggaaca caactgcatc atggagaatc cccagcatcc
    10081 tcaaaccgtt aacacctgca aatgagagct gctttaggcg ggcatttgat cgagcttcct
    10141 ggactttatc tgtcaatgac ttccaggcat cttcaatact ttctgcacag atctgaaagc
    10201 catcatcact ggaaatttca aaaacaagtc ctttcttggg ttttttctca gtaatgcttt
    10261 ccttccgctt ttgttcttgc tgaagccaaa gcatcagtgg ggagctgaaa ttactttcct
    10321 cttcttccac tgttttaggt tctgcacttt cttgttcatt tgctggattt tgggtcacct
    10381 gaacttccgg aagaacagcg acttgccctg caggttgccc acactccttc tgggaggact
    10441 gctccacgct agctgtatcc tgctgctcag cctctgctcc tggagtccct gtgcctgagg
    10501 tcagggatgt cgtttcttgg tctggaatgt gtgcttcaga agaactggtc cgcaaatggg
    10561 aaactttgtg cttcttgcca ttccctttgt ctagaggcag ctgaaaccgt ttggtttttg
    10621 gtttgggttt agtgggaccc ggcactgaag ggcttgctga ccgctgtcca gaagatggag
    10681 aggatggaga acccccagga gaggtggggc tggcttgcac agctgaggat aaagccttgt
    10741 tctgctccag tcccatgcta ttaggagcgt ctaccgtctg ggtaaagttg gatacctggg
    10801 gccctgaagc agaatcaaga tcacgctggg aagaatgaat cccagttttg ctggcaagga
    10861 gctggttcac atgctgaagc tgatagtgtt cgtctgcttc cccagaaggt gaagcggctg
    10921 ttatgcccgt agtctgagtg gaggggagca cacagatgct agatgccgct gttattgcag
    10981 cagtagaggg ggtctgtgat gtccccagtt gtggaaacat tcctgaactt ggcggtaaag
    11041 ccacaggctg gtcctgaatc cccaggctct gctggctagc tttgattaaa agattgctta
    11101 ttgagccaat atctggggta ccaagcaacc ttgggttggt taaggtgacg tgtcctggaa
    11161 ctgacgcact acttgtaggg gttgtggtag tttgcatgga tacaacattc aagacagagg
    11221 aactggatgc caagccagac acagaccctg atgtgaggtg gctagtatcc tggcttattg
    11281 ttgatgtgcc tgccgctgtg gcagcagttc ctcccacact ctgtggtaac aggggtgccg
    11341 gttcaaaata catgatgcta gatttagatc tttttatgat gttggggaca gttcggtgag
    11401 atgaagtatt aagtgacccc aaatctaaca gacttggatg attaggaagc tgggagggtg
    11461 tgaagttaat caatgtcata ttagaaacca catcagaagg ggcaatgttt gaaggggtac
    11521 tagagggagc aagtttttta ttctttcggg tctgtagacg agatatgggt cttttcttga
    11581 gtccagagga tggagtggct actgtggtac tgaggtctgt cctctggctg gattctgaaa
    11641 ccaaaagttg gggatccgga ggaggctgaa ccccaataag caggcctgaa ggaggattgc
    11701 tgatgtttgg tgggaaacta ctttgagtag ctgcagggaa ggaatgtaag tgctggtgat
    11761 gagacatggg tagcaatcct ttgctagcag aagggaacaa agattgagaa gttggcaagc
    11821 ttggatttag tcctgtggta agggtgagac cacctcccat gggtcccaat actgaagtat
    11881 ttgtctccat cacactgggt gtagaactaa cagaagaggt caattggatt ttttgggtca
    11941 ctccatttgg aagagtttgg agaacataaa gtggctgcat gttctggtta acaactatga
    12001 gtttctcagt ggctggcttc aggtggggtg gagtggtctg gacagctgca ttggaaatct
    12061 gagacgggcc aggactatca gtagaattgg gcacatactt ctggttctgg atgggaacag
    12121 ttggggaaac aggtacctga aggccagggg tgctactgtt cctagttaaa tcctgattgt
    12181 tgccatgacc ttgctctact gaaccacaag gagggctaga gatgtggtct gcatccatgt
    12241 gtccttggat aaaatgatca ggagtcatgt ggccttcagg agttggatct actcctgggc
    12301 tcagcagtgc tgggtcactt tcagaggagg ctacagattt ttctgtgatg gttactctct
    12361 tctcccctga gtctgagata acagctagtc tactgggatt ctggctgggg acagtgggac
    12421 tccgggtggt caagacagac agatcagatg gtagctctag aggcaactca aactgttcct
    12481 ctgctgagat agaggaagag acactactat ccacaggttc tgttgtaggc agctgctgag
    12541 aaaatacttc aaaaagaccc atgtcttttt cacgattact gtcaagaccc aatccttcac
    12601 caagattcag gagttctgat gaagatgact ctgggctctc acccaaagcc tgcatggatg
    12661 gagtattctt tagtacaaag tccataatgt ctgaaggcag gatattccca cagtcatcac
    12721 tgttgttatt gtctgaatct gatttcagga gctcagtatt ataggtgtcc agtaaatttt
    12781 tgtcggaggg ggtacttgtg tggactctgc ggcttgactc caatgagctg agctgagctt
    12841 ccaaggaatc ttgtccctgt gttttaactc tgcttacaga atggcagtta tccatctttg
    12901 gctcattttt gtggccaaca gaactcttag tgacatgttc tttctcccca gcatcttcag
    12961 gtctatcaat ctttaagttc tctgttccat tttctttacc atttcttttt ggaatctggc
    13021 tgcttttcct tgttgtggct gtgacactag tatcactctc tgtcccatca tcaacaccat
    13081 ccaactgtga gatttttgga agatcacact gttcctcctc ccgaaataaa ttatgggatg
    13141 ccagtcgttc ctctccacct gaagaaatca ctgttctagt gaagttgtaa tagtataagt
    13201 cgtcttcatc tgaagtgctt aagtcatcgg ccccatccac ctgtccttca gctgatctct
    13261 tgcctcgctt cttcccagtt gagctgctgt agaatggaat gtcttcttca ccataggatc
    13321 ttactccata gagtggggta agcccaaaaa acatgttaga acgtgcacgg gcactgcgac
    13381 ggggatacct ccttttaaaa gagccatcct cctgaggttt ggggccatct ggaagcatta
    13441 ggtttctgtc ctgtactgga agattctgat agttgttact ttgagaatcc tggcatgagg
    13501 tattattggg gcttggttgg gccactggac catctcctgg attttcagag gctgaaattg
    13561 gttctgtggg agatgttgac tctatttgca aaggggaagc aggactactt tctttcaggg
    13621 catttttgga ttgactctca ttttccattt ttagaggtgt cagtgtcact ttgactgtgc
    13681 gtttccgtgg tgcctgtaat tccttggcag atccttctac ttgcatgggt ggagctttgg
    13741 ggactcctgg catagaaagg cctttatcac caatcttatc agaagaaaca ttgttacatg
    13801 gtcgttggcc catatactca ggagtcaaaa cctcatccat aaactctggc ttcaagtttc
    13861 cttcctcacc agttgtcacc tgaccaggtt ccaaaaaaga tttactggaa tgcttttctt
    13921 tgaaagtttc tttacaggat tttttccctt tctgtctcct atctctggaa ctagatccca
    13981 tatgttcgtt gataatggat gatctgttgc tcattccaga tagcttcaag gttttgactt
    14041 cagtatcttc atctggagag gagtttgctg attgggtaga atctgtgtgt tggtctcgat
    14101 cattcctttg ccctctcaaa tggaggtgtg ggaaggagag ggcctcttta gaagaaaacg
    14161 acactgaaga gggttcagca aaggagccga ttttactaac attcagttct ctagatgttg
    14221 tgttatgaac ctgtggggcc agtttaggaa ttccagggta agccacatta tgtgcagatc
    14281 cctctgagct cttggaactc agcactttgg tcttctctcc ctttaaagag gagctcttgg
    14341 acaccaagtc tgaagcactg gactgcttca tttctgaaga tgaagatcca tccaagtgac
    14401 tgtttttatt gcctacagta accactgtcc tttgcaaatt tgaagaggtg gaagtgtttt
    14461 gccctaaact agtacttgaa ttcagtggcc ctaagacatg atcaactact ttggcacttg
    14521 attcaagatc agtagcggtc ccggtggtgg agactgagga aacattattc ctagagtaag
    14581 tattcccagt tctcattgga gacattatcc ggagtttgga ccgctggggt gataaggaag
    14641 aggtactgtg acgcctggag ccaatgcttc gaagtccaga ggagagtaaa ggatcaccta
    14701 ctgtgactat ttcatgagtg gttggggtag gacttcctgc agaaggcaac ggtcgacagc
    14761 caggggatct ctgtgttgga gaataactgg gtgttcgaat cctggggacc tttgagatga
    14821 catgataata acaggagcca gaggtttgtg aatgaggagg tcggtctggt gatggaggac
    14881 ttataatttc agctgtgttt tgactctctt ttgatgaact ttctgtaaaa gatgttggac
    14941 tatgggcaat ggtcctgttt tcatcatgtt caacagtgct gttgatatcc ggctctacga
    15001 ctggaggacg gcactccact atcttgcatg tatatacaca gcgcttgcga gcatctgtgg
    15061 tgctccagta taccctggaa cactgatatc caataggaaa gagcttatct tcacagtcgg
    15121 agagatcatt tagaattcct aagcagtcga ttgtcataga cccaatcatc atgtggatat
    15181 tttctggttc caagccattg agaaacttcc ttctcaagct gattccttca aagtccacaa
    15241 acactcttct gaaaacttca aatccattct caggaaccac ttcgcctttg atcaaatccc
    15301 gatgtcgttg gcaatatact tttttatcat ccagaaagac acagttcttg gctcgggaac
    15361 acatgaagtg atagttgctg gtgcaggatg tgagacagca acccacggtg gctcctggct
    15421 tttggcagaa ttcacatctc agctgcttgc ccctgatcac agccatatgc acattcttta
    15481 gtgatccgtc atcatcttca aacacttccg ctgaccacaa agcacaattt acatgtgtcc
    15541 actcattttg gccaatatat agtaaacgac cagcatcatt agcactgtca tcaccataag
    15601 tcaaacataa cgcacactgt ctattgtctt ctatgcctgg gggtgggttc agctctggac
    15661 tgtcttctcg actcctatca gtactcaaaa ttggtggtgt aggaggatgc agaggagttg
    15721 gtgagtctgg ttctccagga cctttgggtt tgggagctgg aatgattttc ttcattaaag
    15781 gaggctgctc agtgtggctg ttttcctctc gctcctgcca ctgagcataa ttatggtcaa
    15841 gtgaaggtgg aagcactgcg tttggtaaca tcccactgtt gcttgatact ttatttggct
    15901 cccaaaacct ggactttttg acactgaacc atggaaaaac acgttccatt tgccgaatga
    15961 agaaggactt gaccatgctg ttggcttttt taatttctgg ctgtcctcca tctgaattaa
    16021 tggctgcttg aatgatcttc acaatatcat cactgaactc caacacagat gtgtaattcc
    16081 cttggtccat cttcctcttg actccttcta gatctaaagg ctgctgatca tcctgtttgc
    16141 tgacctcagt aagaactggt ggatcaggtc cttcggggga gctgcgggaa ggtatactct
    16201 cctctgtctc gggatttaag tctggaggct tggcagcctg ccggtagcgt agcaaatggc
    16261 tggtagtccg agaattcaac aaagctgtca gaacttgctt cagagaaatc tgcagctctt
    16321 tttcaagggc cagtcgccac tctgcagggt gccgctcagt acagttcaca caagtgtagg
    16381 ccacactttc tggcagatta gatagaatct catacatctc atctgaaaga ttctcacatt
    16441 tggaatggac ccagcgatca cactttccac attgcatcat cttactctca tagtcatcat
    16501 catcataaca tttgtcacag agagggcaga agtttccttt agcaaagagc ttggcgcaat
    16561 catgacacag tgagaaatca tgagaccact gtgcatccca ccctttgcct ggagttgtgg
    16621 atccacagct cttacagcga acacacttgg tacagatcca gactttcttc ttcttt
//
