LOCUS       sequence-47680-e         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Young CL, Raden DL, Caplan JL, Czymmek KJ, Robinson AS
  TITLE     Cassette series designed for live-cell imaging of proteins and 
            high-resolution techniques in yeast.
  JOURNAL   Yeast. 2012 Mar;29(3-4):119-36. doi: 10.1002/yea.2895. Epub 2012 Apr
            4.
  PUBMED    22473760
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast."; 
            date: "2012-03"; volume: "29(3-4):119-36. doi"; pages: " 
            10.1002/yea.2895. Epub 2012 Apr 4"
COMMENT     Sequence Label: pCY 3040-04
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(123..797)
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
ORIGIN
        1 tctgatatca tcgatgaatt cgagctcggg tattttctcc ttacgcatct gtgcggtatt
       61 tcacaccgca tagatcggca agtgcacaaa caatacttaa ataaatacta ctcagtaata
      121 acctatttct tagcattttt gacgaaattt gctattttgt tagagtcttt tacaccattt
      181 gtctccacac ctccgcttac atcaacacca ataacgccat ttaatctaag cgcatcacca
      241 acattttctg gcgtcagtcc accagctaac ataaaatgta agctttcggg gctctcttgc
      301 cttccaaccc agtcagaaat cgagttccaa tccaaaagtt cacctgtccc acctgcttct
      361 gaatcaaaca agggaataaa cgaatgaggt ttctgtgaag ctgcactgag tagtatgttg
      421 cagtcttttg gaaatacgag tcttttaata actggcaaac cgaggaactc ttggtattct
      481 tgccacgact catctccatg cagttggacg atatcaatgc cgtaatcatt gaccagagcc
      541 aaaacatcct ccttaggttg attacgaaac acgccaacca agtatttcgg agtgcctgaa
      601 ctatttttat atgcttttac aagacttgaa attttccttg caataaccgg gtcaattgtt
      661 ctctttctat tgggcacaca tataataccc agcaagtcag catcggaatc tagagcacat
      721 tctgcggcct ctgtgctctg caagccgcaa actttcacca atggaccaga actacctgtg
      781 aaattaataa cagacatact ccaagctgcc tttgtgtgct taatcacgta tactcacgtg
      841 ctcaatagtc
//
