LOCUS       sequence-476819-         995 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses mScarlet-i and SBP tagged human SPARC for protein 
            visualisation and use in the RUSH trafficking system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 995)
  AUTHORS   Thompson G, Hoyle A, Lewis PA, Prada-Sanchez ME, Swift J, Heesom K, 
            Lowe M, Stephens D, Stevenson NL
  TITLE     Multiple golgins are required to support extracellular matrix 
            secretion, modification, and assembly.
  JOURNAL   J Cell Biol. 2025 Oct 6;224(10):e202411167. doi: 
            10.1083/jcb.202411167. Epub 2025 Aug 18.
  PUBMED    40824304
REFERENCE   2  (bases 1 to 995)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2025-10-6"; volume: "224(10):e202411167. doi"; pages:
            " 10.1083/jcb.202411167. Epub 2025 Aug 18"
COMMENT     Sequence Label: pLVXNeo_SPARC-mSc-SBP
FEATURES             Location/Qualifiers
     source          1..995
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     15..39
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 gtctatataa gcagagctcg tttagtgaac cgtcagatca gcaccatgag ggcctggata
       61 ttctttctcc tttgcctggc cgggagggcg ttggcagccc ctcagcaaga agcactacct
      121 gatgagacag aggttgttga agaaactgtc gcagaggtga ctgaggtatc tgtcggagct
      181 aatcctgtcc aggttgaagt aggagaattt gatgatggtg cagaggaaac ggaagaagag
      241 gtggtggcgg aaaatccctg ccaaaatcat cactgcaaac acggaaaagt gtgtgagtta
      301 gatgagaaca atactcccat gtgcgtgtgt caggacccaa ctagctgccc agcccctatt
      361 ggcgagtttg agaaagtctg tagcaatgac aacaagacct tcgattctag ttgtcatttt
      421 tttgccacaa agtgcacttt ggagggcacg aagaagggtc acaagctcca tctggactac
      481 atcgggcctt gtaaatacat tcccccttgt ctggattctg agctgaccga attccccctg
      541 cgcatgcggg attggctcaa gaacgtcttg gtcactctgt atgagaggga tgaagacaac
      601 aaccttctga ctgagaagca gaagttacgg gttaagaaga tccatgagaa tgagaaacgc
      661 ctggaggcag gagaccaccc agtggagcta ttagcccggg actttgagaa aaattataac
      721 atgtatattt ttcctgtaca ctggcagttc gggcagcttg accagcatcc aattgacggg
      781 tacctctcgc acaccgagct ggctccatta cgtgctccgc tcatccccat ggaacattgc
      841 acaactagat tttttgaaac gtgtgacctg gacaatgaca agtacatcgc tctcgatgag
      901 tgggctggct gctttggaat caaacaaaag gatatcgaca aggatcttgt cataggaagt
      961 gctggctccg ctgctggttc tggcgaattc atgga
//
