LOCUS       sequence-476821-        9055 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses CDK11B p58 isoform in mammalian cell lines.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9055)
  AUTHORS   Julian LA, Crozier L, Lukow D, Mishra S, Swamy A, Hagenson RA, 
            Sennhenn P, Sausville EL, Mendelson B, Chuaqui C, Qiao L, Vasudevan 
            A, Lin K-T, Bhatia S, Bertomeu T, Chatr-aryamontri A, Zhang L, Rees 
            MG, Ronan MM, Roth JA, Nottoli T, Bai S, Lakshmipathi J, Muthusamy 
            V, Van Vranken JG, Gygi SP, Thompson SL, Smith JC, Anderson K, Shah 
            S, Bindra RS, Akerman M, Spector DL, Krainer AR, Sheltzer JM
  TITLE     On-target toxicity limits the efficacy of CDK11 inhibition against 
            cancers with 1p36 deletions
  JOURNAL   bioRxiv 2025.08.03.668359; doi: 
            https://doi.org/10.1101/2025.08.03.668359
REFERENCE   2  (bases 1 to 9055)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.08.03.668359; doi: https://doi.org/10.1101/2025.08.03.668359"
COMMENT     Sequence Label: pLV[Exp]-Bsd-CMV>hCDK11B-p58
FEATURES             Location/Qualifiers
     source          1..9055
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..105
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(106..174,175..966)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(324..343)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1137..1725
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1626..1645
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1879..1896
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2013..2034
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2049..2054,2055..2072,2073..2079)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2087..2103
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2092..2114
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2111..2127
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2111..2127
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2146..2166
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2148..2166
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2194..2420
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2421..2601
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2648..2773
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3266..3499
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3684..3728
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3995..4111
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        4134..4437
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4438..4641
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4592..4612
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4638..4662
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    complement(6040..6164)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    6195..6783
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6248..6268)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6666..6677)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6834..6854)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6839..7168
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7019..7154
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        7216..7263
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7282..7680
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             7767..8000
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8072..8193
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8109..8128)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8163..8182
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8233..8368
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8388..8407)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8389..8407)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8417..8434)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8417..8433)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8426..8448)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8575..9030
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8662..8681)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8872..8893
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
       61 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
      121 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
      181 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
      241 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
      301 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
      361 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
      421 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
      481 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
      541 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
      601 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
      661 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
      721 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
      781 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
      841 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
      901 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
      961 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     1021 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     1081 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     1141 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     1201 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     1261 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     1321 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     1381 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     1441 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     1501 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     1561 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     1621 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     1681 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     1741 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     1801 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     1861 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
     1921 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
     1981 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
     2041 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
     2101 caatttcaca caggaaacag ctatgaccat gattacgcca agcgcgcaat taaccctcac
     2161 taaagggaac aaaagctgga gctgcaagct taatgtagtc ttatgcaata ctcttgtagt
     2221 cttgcaacat ggtaacgatg agttagcaac atgccttaca aggagagaaa aagcaccgtg
     2281 catgccgatt ggtggaagta aggtggtacg atcgtgcctt attaggaagg caacagacgg
     2341 gtctgacatg gattggacga accactgaat tgccgcattg cagagatatt gtatttaagt
     2401 gcctagctcg atacataaac gggtctctct ggttagacca gatctgagcc tgggagctct
     2461 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     2521 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     2581 cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc
     2641 agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg
     2701 gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg
     2761 gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt
     2821 aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct
     2881 agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact
     2941 gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac
     3001 agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt
     3061 agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccgctga
     3121 tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata
     3181 aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc
     3241 agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag
     3301 gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg
     3361 gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc
     3421 aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc
     3481 taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg
     3541 ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga
     3601 cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg
     3661 aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg
     3721 caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa
     3781 tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata
     3841 gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac
     3901 ccgacaggcc cgaaggaata gaagaagaag gtggagagag agacagagac agatccattc
     3961 gattagtgaa cggatctcga cggtatcggt taacttttaa aagaaaaggg gggattgggg
     4021 ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat
     4081 tacaaaaaca aattacaaaa ttcaaaattt tatcgataag cttgggagtt ccgcgttaca
     4141 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
     4201 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
     4261 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
     4321 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
     4381 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg
     4441 atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca
     4501 agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt
     4561 ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg
     4621 gaggtctata taagcagagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca
     4681 cgctgttttg acctccatag aagacaccga ctcatgagtg aagatgaaga acgagaaaat
     4741 gaaaaccacc tcttggttgt tccagagtca cggttcgacc gagattccgg ggagagtgaa
     4801 gaagcagagg aagaagtggg tgagggaacg ccgcagagca gcgccctgac agagggcgac
     4861 tatgtgcccg actcccctgc cctgtcgccc atcgagctca agcaggagct gcccaagtac
     4921 ctgccggccc tgcagggctg ccggagcgtc gaggagttcc agtgcctgaa caggatcgag
     4981 gagggcacct atggagtggt ctacagagca aaagacaaga aaacagatga aattgtggct
     5041 ctaaagcggc tgaagatgga gaaggagaag gagggcttcc cgatcacgtc gctgagggag
     5101 atcaacacca tcctcaaggc ccagcatccc aacatcgtca ccgttagaga gattgtggtg
     5161 ggcagcaaca tggacaagat ctacatcgtg atgaactatg tggagcacga cctcaagagc
     5221 ctgatggaga ccatgaaaca gcccttcctg ccaggggagg tgaagaccct gatgatccag
     5281 ctgctgcgtg gggtgaaaca cctgcacgac aactggatcc tgcaccgtga cctcaagacg
     5341 tccaacctgc tgctgagcca cgccggcatc ctcaaggtgg gtgacttcgg gctggcgcgg
     5401 gagtacggat cccctctgaa ggcctacacc ccggtcgtgg tgaccctgtg gtaccgcgcc
     5461 ccagagctgc tgcttggtgc caaggaatac tccacggccg tggacatgtg gtcagtgggt
     5521 tgcatcttcg gggagctgct gactcagaag cctctgttcc ccgggaagtc agaaatcgat
     5581 cagatcaaca aggtgttcaa ggatctgggg acccctagtg agaaaatctg gcccggctac
     5641 agcgagctcc cagcagtcaa gaagatgacc ttcagcgagc acccctacaa caacctccgc
     5701 aagcgcttcg gggctctgct ctcagaccag ggcttcgacc tcatgaacaa gttcctgacc
     5761 tacttccccg ggaggaggat cagcgctgag gacggcctca agcatgagta tttccgcgag
     5821 acccccctcc ccatcgaccc ctccatgttc cccacgtggc ccgccaagag cgagcagcag
     5881 cgtgtgaagc ggggcaccag cccgaggccc cctgagggag gcctgggcta cagccagctg
     5941 ggtgacgacg acctgaagga gacgggcttc caccttacca ccacgaacca gggggcctct
     6001 gccgcgggcc ccggcttcag cctcaagttc tgaatcgacc atagtgactg gatatgttgt
     6061 gttttacagt attatgtagt ctgtttttta tgcaaaatct aatttaatat attgatattt
     6121 atatcatttt acgtttctcg ttcagctttc ttgtacaaag tggttgatat ccagcacagt
     6181 ggcggccgct cgacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     6241 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     6301 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     6361 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     6421 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
     6481 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     6541 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct
     6601 ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
     6661 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
     6721 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
     6781 cgcctggaat tctgcagata tccggttagt aatgagtttg gaattaattc tgtggaatgt
     6841 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     6901 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
     6961 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
     7021 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     7081 tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg
     7141 cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg
     7201 atctgatcag cacgtgttga caattaatca tcggcatagt atatcggcat agtataatac
     7261 gacaaggtga ggaactaaac catggccaag cctttgtctc aagaagaatc caccctcatt
     7321 gaaagagcaa cggctacaat caacagcatc cccatctctg aagactacag cgtcgccagc
     7381 gcagctctct ctagcgacgg ccgcatcttc actggtgtca atgtatatca ttttactggg
     7441 ggaccttgtg cagaactcgt ggtgctgggc actgctgctg ctgcggcagc tggcaacctg
     7501 acttgtatcg tcgcgatcgg aaatgagaac aggggcatct tgagcccctg cggacggtgc
     7561 cgacaggtgc ttctcgatct gcatcctggg atcaaagcca tagtgaagga cagtgatgga
     7621 cagccgacgg cagttgggat tcgtgaattg ctgccctctg gttatgtgtg ggagggctaa
     7681 gcacaattcg agctcggtac ctttaagacc aatgacttac aaggcagctg tagatcttag
     7741 ccacttttta aaagaaaagg ggggactgga agggctagct cactcccaac gaagacaaga
     7801 tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     7861 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     7921 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     7981 agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt atttataact
     8041 tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta
     8101 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     8161 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc
     8221 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     8281 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
     8341 aagtagtgag gaggcttttt tggaggccta gggacgtacc caattcgccc tatagtgagt
     8401 cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg
     8461 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag
     8521 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc
     8581 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     8641 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     8701 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     8761 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
     8821 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     8881 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
     8941 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     9001 attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa atgtg
//
