LOCUS       sequence-476824-       20220 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This all-in-one vector is used to specifically silence the GoPGF 
            gene via virus-induced gene silencing (VIGS)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 20220)
  AUTHORS   Hao Y, Yuan Z, Li Y, Zuo D, Cheng H, Wang Q, Zhang Y, Lv L, Liu J, 
            Song G
  TITLE     An all-in-one plant virus-based vector toolkit for streamlined gene 
            silencing, overexpression, and genome editing.
  JOURNAL   Plant Commun. 2025 May 19:101379. doi: 10.1016/j.xplc.2025.101379.
  PUBMED    40394900
REFERENCE   2  (bases 1 to 20220)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Commun. 2025 May 19:101379. doi: 10.1016/j.xplc.2025.101379."
COMMENT     Sequence Label: pVS2-GoPGF
FEATURES             Location/Qualifiers
     source          1..20220
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1085..1102
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      1137..1389
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(1398..1414)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1398..1414)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     misc_feature    1435..1459
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             1924..2574
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     primer_bind     complement(2762..2779)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(3013..3032)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     oriT            3255..3364
                     /note="incP origin of transfer"
     CDS             3397..3768
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     rep_origin      3895..4606
                     /label=oriV
                     /note="incP origin of replication"
     mobile_element  5229..5996
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     CDS             6188..6982
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     CDS             7281..8429
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKTYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    10025..10049
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     primer_bind     complement(10296..10317)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     10508..10527
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10652..10674
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     10666..10683
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     10667..10683
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(10692..10944)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        complement(17798..18143)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     promoter        18673..18719
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
ORIGIN
        1 acgctgtttg agggaaaagt agagaacgta tttgttttta tgttcaggcg gttcttgtgt
       61 gtcaacaaag atggacattg ttactcaagg aagcacgatg agctttatta ttacggacga
      121 gtggacttag attctgtgag taaggttacc gaattggcca gttaacgcta gcgaatcgtt
      181 tgatacactg gcatggtgaa ctggtagctc taggtctcga agtgcatcca tcaatcgtgc
      241 agctggatga tcgatatctg gacttcgaac ccgaatcatg gcttcggatc ctactatctt
      301 cacatcaact tccactgtct ttggccaata agtagaggat tgtttcatgg tattgtcgaa
      361 tgcggatttg gtagtttggt tttcgaaaac atttatgggg ttcaacttgg atttttcaga
      421 ttgtactagg agttgagcct ctagtttatc gatttttgat cttagttcct tgatgtaggc
      481 tactgcatcc gaaagtaatg aggctttgtc catcttggat acgtttggaa ccacggaacg
      541 aagtgcgtag aaacgatggt tcagtctctc acgtcgttgc ctttctgctt caacgactag
      601 tctgcaggca tgccattcga ggcccgggca tgtcccgaag acattaaact acggttcttt
      661 aagtagatcc gtgtctgaag ttttaggttc aatttaaacc tacgagattg acattctcga
      721 ctgatcttga ttgatcggta agtcttttgt aatttaattt tctttttgat tttattttaa
      781 attgttatct gtttctgtgt atagactgtt tgagatcggc gtttggccga ctcattgtct
      841 taccataggg gaacggactt tgtttgtgtt gttattttat ttgtatttta ttaaaattct
      901 caacgatctg aaaaagcctc gcggctaaga gattgttggg gggtgagtaa gtacttttaa
      961 agtgatgatg gttacaaagg caaaaggggt aaaacccctc gcctacgtaa gcgttattac
     1021 gcccgtctgt acttatatca gtacactgac gagtccctaa aggacgaaac gggagaacgc
     1081 tagccaccac caccaccacc acgtgtgaat tacaggtgac cagctcgaat ttccccgatc
     1141 gttcaaacat ttggcaataa agtttcttaa gattgaatcc tgttgccggt cttgcgatga
     1201 ttatcatata atttctgttg aattacgtta agcatgtaat aattaacatg taatgcatga
     1261 cgttatttat gagatgggtt tttatgatta gagtcccgca attatacatt taatacgcga
     1321 tagaaaacaa aatatagcgc gcaaactagg ataaattatc gcgcgcggtg tcatctatgt
     1381 tactagatcg ggaattggtc atagctgttt cctgggatcc aaactatcag tgtttgacag
     1441 gatatattgg cgggtaaacc taagagaaaa gagcgtttat tagaataatc ggatatttaa
     1501 aagggcgtga aaaggtttat ccgttcgtcc atttgtatgt gcatgccaac cacagggttc
     1561 cccagatctg gcgccggcca gcgagacgag caagattggc cgccgcccga aacgatccga
     1621 cagcgcgccc agcacaggtg cgcaggcaaa ttgcaccaac gcatacagcg ccagcagaat
     1681 gccatagtgg gcggtgacgt cgttcgagtg aaccagatcg cgcaggaggc ccggcagcac
     1741 cggcataatc aggccgatgc cgacagcgtc gagcgcgaca gtgctcagaa ttacgatcag
     1801 gggtatgttg ggtttcacgt ctggcctccg gaccagcctc cgctggtccg attgaacgcg
     1861 cggattcttt atcactgata agttggtgga catattatgt ttatcagtga taaagtgtca
     1921 agcatgacaa agttgcagcc gaatacagtg atccgtgccg ccctggacct gttgaacgag
     1981 gtcggcgtag acggtctgac gacacgcaaa ctggcggaac ggttgggggt tcagcagccg
     2041 gcgctttact ggcacttcag gaacaagcgg gcgctgctcg acgcactggc cgaagccatg
     2101 ctggcggaga atcatacgca ttcggtgccg agagccgacg acgactggcg ctcatttctg
     2161 atcgggaatg cccgcagctt caggcaggcg ctgctcgcct accgcgatgg cgcgcgcatc
     2221 catgccggca cgcgaccggg cgcaccgcag atggaaacgg ccgacgcgca gcttcgcttc
     2281 ctctgcgagg cgggtttttc ggccggggac gccgtcaatg cgctgatgac aatcagctac
     2341 ttcactgttg gggccgtgct tgaggagcag gccggcgaca gcgatgccgg cgagcgcggc
     2401 ggcaccgttg aacaggctcc gctctcgccg ctgttgcggg ccgcgataga cgccttcgac
     2461 gaagccggtc cggacgcagc gttcgagcag ggactcgcgg tgattgtcga tggattggcg
     2521 aaaaggaggc tcgttgtcag gaacgttgaa ggaccgagaa agggtgacga ttgatcagga
     2581 ccgctgccgg agcgcaaccc actcactaca gcagagccat gtagacaaca tcccctcccc
     2641 ctttccaccg cgtcagacgc ccgtagcagc ccgctacggg ctttttcatg ccctgcccta
     2701 gcgtccaagc ctcacggccg cgctcggcct ctctggcggc cttctggcgc tcttccgctt
     2761 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     2821 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     2881 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     2941 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     3001 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     3061 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     3121 ttttccgctg cataaccctg cttcggggtc attatagcga ttttttcggt atatccatcc
     3181 tttttcgcac gatatacagg attttgccaa agggttcgtg tagactttcc ttggtgtatc
     3241 caacggcgtc agccgggcag gataggtgaa gtaggcccac ccgcgagcgg gtgttccttc
     3301 ttcactgtcc cttattcgca cctggcggtg ctcaacggga atcctgctct gcgaggctgg
     3361 ccggctaccg ccggcgtaac agatgagggc aagcggatgg ctgatgaaac caagccaacc
     3421 aggaagggca gcccacctat caaggtgtac tgccttccag acgaacgaag agcgattgag
     3481 gaaaaggcgg cggcggccgg catgagcctg tcggcctacc tgctggccgt cggccagggc
     3541 tacaaaatca cgggcgtcgt ggactatgag cacgtccgcg agctggcccg catcaatggc
     3601 gacctgggcc gcctgggcgg cctgctgaaa ctctggctca ccgacgaccc gcgcacggcg
     3661 cggttcggtg atgccacgat cctcgccctg ctggcgaaga tcgaagagaa gcaggacgag
     3721 cttggcaagg tcatgatggg cgtggtccgc ccgagggcag agccatgact tttttagccg
     3781 ctaaaacggc cggggggtgc gcgtgattgc caagcacgtc cccatgcgct ccatcaagaa
     3841 gagcgacttc gcggagctgg tgaagtacat caccgacgag caaggcaaga ccgagcgcct
     3901 ttccgacgct caccgggctg gttgccctcg ccgctgggct ggcggccgtc tatggccctg
     3961 caaacgcgcc agaaacgccg tcgaagccgt gtgcgagaca ccgcggccgc cggcgttgtg
     4021 gatacctcgc ggaaaacttg gccctcactg acagatgagg ggcggacgtt gacacttgag
     4081 gggccgactc acccggcgcg gcgttgacag atgaggggca ggctcgattt cggccggcga
     4141 cgtggagctg gccagcctcg caaatcggcg aaaacgcctg attttacgcg agtttcccac
     4201 agatgatgtg gacaagcctg gggataagtg ccctgcggta ttgacacttg aggggcgcga
     4261 ctactgacag atgaggggcg cgatccttga cacttgaggg gcagagtgct gacagatgag
     4321 gggcgcacct attgacattt gaggggctgt ccacaggcag aaaatccagc atttgcaagg
     4381 gtttccgccc gtttttcggc caccgctaac ctgtctttta acctgctttt aaaccaatat
     4441 ttataaacct tgtttttaac cagggctgcg ccctgtgcgc gtgaccgcgc acgccgaagg
     4501 ggggtgcccc cccttctcga accctcccgg cccgctaacg cgggcctccc atccccccag
     4561 gggctgcgcc cctcggccgc gaacggcctc accccaaaaa tggcagcgct ggcagtcctt
     4621 gccattgccg ggatcggggc agtaacggga tgggcgatca gcccgagcgc gacgcccgga
     4681 agcattgacg tgccgcaggt gctggcatcg acattcagcg accaggtgcc gggcagtgag
     4741 ggcggcggcc tgggtggcgg cctgcccttc acttcggccg tcggggcatt cacggacttc
     4801 atggcggggc cggcaatttt taccttgggc attcttggca tagtggtcgc gggtgccgtg
     4861 ctcgtgttcg ggggtgcgat aaacccagcg aaccatttga ggtgataggt aagattatac
     4921 cgaggtatga aaacgagaat tggaccttta cagaattact ctatgaagcg ccatatttaa
     4981 aaagctacca agacgaagag gatgaagagg atgaggaggc agattgcctt gaatatattg
     5041 acaatactga taagataata tatcttttat atagaagata tcgccgtatg taaggatttc
     5101 agggggcaag gcataggcag cgcgcttatc aatatatcta tagaatgggc aaagcataaa
     5161 aacttgcatg gactaatgct tgaaacccag gacaataacc ttatagcttg taaattctat
     5221 cataattggg taatgactcc aacttattga tagtgtttta tgttcagata atgcccgatg
     5281 actttgtcat gcagctccac cgattttgag aacgacagcg acttccgtcc cagccgtgcc
     5341 aggtgctgcc tcagattcag gttatgccgc tcaattcgct gcgtatatcg cttgctgatt
     5401 acgtgcagct ttcccttcag gcgggattca tacagcggcc agccatccgt catccatatc
     5461 accacgtcaa agggtgacag caggctcata agacgcccca gcgtcgccat agtgcgttca
     5521 ccgaatacgt gcgcaacaac cgtcttccgg agactgtcat acgcgtaaaa cagccagcgc
     5581 tggcgcgatt tagccccgac atagccccac tgttcgtcca tttccgcgca gacgatgacg
     5641 tcactgcccg gctgtatgcg cgaggttacc gactgcggcc tgagtttttt aagtgacgta
     5701 aaatcgtgtt gaggccaacg cccataatgc gggctgttgc ccggcatcca acgccattca
     5761 tggccatatc aatgattttc tggtgcgtac cgggttgaga agcggtgtaa gtgaactgca
     5821 gttgccatgt tttacggcag tgagagcaga gatagcgctg atgtccggcg gtgcttttgc
     5881 cgttacgcac caccccgtca gtagctgaac aggagggaca gctgatagaa acagaagcca
     5941 ctggagcacc tcaaaaacac catcatacac taaatcagta agttggcagc atcacccata
     6001 attgtggttt caaaatcggc tccgtcgata ctatgttata cgccaacttt gaaaacaact
     6061 ttgaaaaagc tgttttctgg tatttaaggt tttagaatgc aaggaacagt gaattggagt
     6121 tcgtcttgtt ataattagct tcttggggta tctttaaata ctgtagaaaa gaggaaggaa
     6181 ataataaatg gctaaaatga gaatatcacc ggaattgaaa aaactgatcg aaaaataccg
     6241 ctgcgtaaaa gatacggaag gaatgtctcc tgctaaggta tataagctgg tgggagaaaa
     6301 tgaaaaccta tatttaaaaa tgacggacag ccggtataaa gggaccacct atgatgtgga
     6361 acgggaaaag gacatgatgc tatggctgga aggaaagctg cctgttccaa aggtcctgca
     6421 ctttgaacgg catgatggct ggagcaatct gctcatgagt gaggccgatg gcgtcctttg
     6481 ctcggaagag tatgaagatg aacaaagccc tgaaaagatt atcgagctgt atgcggagtg
     6541 catcaggctc tttcactcca tcgacatatc ggattgtccc tatacgaata gcttagacag
     6601 ccgcttagcc gaattggatt acttactgaa taacgatctg gccgatgtgg attgcgaaaa
     6661 ctgggaagaa gacactccat ttaaagatcc gcgcgagctg tatgattttt taaagacgga
     6721 aaagcccgaa gaggaacttg tcttttccca cggcgacctg ggagacagca acatctttgt
     6781 gaaagatggc aaagtaagtg gctttattga tcttgggaga agcggcaggg cggacaagtg
     6841 gtatgacatt gccttctgcg tccggtcgat cagggaggat atcggggaag aacagtatgt
     6901 cgagctattt tttgacttac tggggatcaa gcctgattgg gagaaaataa aatattatat
     6961 tttactggat gaattgtttt agtacctaga tgtggcgcaa cgatgccggc gacaagcagg
     7021 agcgcaccga cttcttccgc atcaagtgtt ttggctctca ggccgaggcc cacggcaagt
     7081 atttgggcaa ggggtcgctg gtattcgtgc agggcaagat tcggaatacc aagtacgaga
     7141 aggacggcca gacggtctac gggaccgact tcattgccga taaggtggat tatctggaca
     7201 ccaaggcacc aggcgggtca aatcaggaat aagggcacat tgccccggcg tgagtcgggg
     7261 caatcccgca aggagggtga atgaatcgga cgtttgaccg gaagacatac aggcaagaac
     7321 tgatcgacgc ggggttttcc gccgaggatg ccgaaaccat cgcaagccgc accgtcatgc
     7381 gtgcgccccg cgaaaccttc cagtccgtcg gctcgatggt ccagcaagct acggccaaga
     7441 tcgagcgcga cagcgtgcaa ctggctcccc ctgccctgcc cgcgccatcg gccgccgtgg
     7501 agcgttcgcg tcgtctcgaa caggaggcgg caggtttggc gaagtcgatg accatcgaca
     7561 cgcgaggaac tatgacgacc aagaagcgaa aaaccgccgg cgaggacctg gcaaaacagg
     7621 tcagcgaggc caagcaggcc gcgttgctga aacacacgaa gcagcagatc aaggaaatgc
     7681 agctttcctt gttcgatatt gcgccgtggc cggacacgat gcgagcgatg ccaaacgaca
     7741 cggcccgctc tgccctgttc accacgcgca acaagaaaat cccgcgcgag gcgctgcaaa
     7801 acaaggtcat tttccacgtc aacaaggacg tgaagatcac ctacaccggc gtcgagctgc
     7861 gggccgacga tgacgaactg gtgtggcagc aggtgttgga gtacgcgaag cgcaccccta
     7921 tcggcgagcc gatcaccttc acgttctacg agctttgcca ggacctgggc tggtcgatca
     7981 atggccggta ttacacgaag gccgaggaat gcctgtcgcg cctacaggcg acggcgatgg
     8041 gcttcacgtc cgaccgcgtt gggcacctgg aatcggtgtc gctgctgcac cgcttccgcg
     8101 tcctggaccg tggcaagaaa acgtcccgtt gccaggtcct gatcgacgag gaaatcgtcg
     8161 tgctgtttgc tggcgaccac tacacgaaat tcatatggga gaagtaccgc aagctgtcgc
     8221 cgacggcccg acggatgttc gactatttca gctcgcaccg ggagccgtac ccgctcaagc
     8281 tggaaacctt ccgcctcatg tgcggatcgg attccacccg cgtgaagaag tggcgcgagc
     8341 aggtcggcga agcctgcgaa gagttgcgag gcagcggcct ggtggaacac gcctgggtca
     8401 atgatgacct ggtgcattgc aaacgctagg gccttgtggg gtcagttccg gctgggggtt
     8461 cagcagccag cgctttactg gcatttcagg aacaagcggg cactgctcga cgcacttgct
     8521 tcgctcagta tcgctcggga cgcacggcgc gctctacgaa ctgccgataa acagaggatt
     8581 aaaattgaca attgtgatta aggctcagat tcgacggctt ggagcggccg acgtgcagga
     8641 tttccgcgag atccgattgt cggccctgaa gaaagctcca gagatgttcg ggtccgttta
     8701 cgagcacgag gagaaaaagc ccatggaggc gttcgctgaa cggttgcgag atgccgtggc
     8761 attcggcgcc tacatcgacg gcgagatcat tgggctgtcg gtcttcaaac aggaggacgg
     8821 ccccaaggac gctcacaagg cgcatctgtc cggcgttttc gtggagcccg aacagcgagg
     8881 ccgaggggtc gccggtatgc tgctgcgggc gttgccggcg ggtttattgc tcgtgatgat
     8941 cgtccgacag attccaacgg gaatctggtg gatgcgcatc ttcatcctcg gcgcacttaa
     9001 tatttcgcta ttctggagct tgttgtttat ttcggtctac cgcctgccgg gcggggtcgc
     9061 ggcgacggta ggcgctgtgc agccgctgat ggtcgtgttc atctctgccg ctctgctagg
     9121 tagcccgata cgattgatgg cggtcctggg ggctatttgc ggaactgcgg gcgtggcgct
     9181 gttggtgttg acaccaaacg cagcgctaga tcctgtcggc gtcgcagcgg gcctggcggg
     9241 ggcggtttcc atggcgttcg gaaccgtgct gacccgcaag tggcaacctc ccgtgcctct
     9301 gctcaccttt accgcctggc aactggcggc cggaggactt ctgctcgttc cagtagcttt
     9361 agtgtttgat ccgccaatcc cgatgcctac aggaaccaat gttctcggcc tggcgtggct
     9421 cggcctgatc ggagcgggtt taacctactt cctttggttc cgggggatct cgcgactcga
     9481 acctacagtt gtttccttac tgggctttct cagccccaga tctggggtcg atcagccggg
     9541 gatgcatcag gccgacagtc ggaacttcgg gtccccgacc tgtaccattc ggtgagcaat
     9601 ggatagggga gttgatatcg tcaacgttca cttctaaaga aatagcgcca ctcagcttcc
     9661 tcagcggctt tatccagcga tttcctatta tgtcggcata gttctcaaga tcgacagcct
     9721 gtcacggtta agcgagaaat gaataagaag gctgataatt cggatctctg cgagggagat
     9781 gatatttgat cacaggcagc aacgctctgt catcgttaca atcaacatgc taccctccgc
     9841 gagatcatcc gtgtttcaaa cccggcagct tagttgccgt tcttccgaat agcatcggta
     9901 acatgagcaa agtctgccgc cttacaacgg ctctcccgct gacgccgtcc cggactgatg
     9961 ggctgcctgt atcgagtggt gattttgtgc cgagctgccg gtcggggagc tgttggctgg
    10021 ctggtggcag gatatattgt ggtgtaaaca aattgacgct tagacaactt aataacacat
    10081 tgcggacgtt tttaatgtac tggggtggtt tttcttttca ccagtgagac gggcaacagc
    10141 tgattgccct tcaccgcctg gccctgagag agttgcagca agcggtccac gctggtttgc
    10201 cccagcaggc gaaaatcctg tttgatggtg gttccgaaat cggcaaaatc ccttataaat
    10261 caaaagaata gcccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat
    10321 taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac
    10381 tacgtgaacc atcacccaaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc
    10441 ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga
    10501 gaaaggaagg gaagaaagcg aaaggagcgg gcgccattca ggctgcgcaa ctgttgggaa
    10561 gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca
    10621 aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc
    10681 agtgaattcc cgatctagta acatagatga caccgcgcgc gataatttat cctagtttgc
    10741 gcgctatatt ttgttttcta tcgcgtatta aatgtataat tgcgggactc taatcataaa
    10801 aacccatctc ataaataacg tcatgcatta catgttaatt attacatgct taacgtaatt
    10861 caacagaaat tatatgataa tcatcgcaag accggcaaca ggattcaatc ttaagaaact
    10921 ttattgccaa atgtttgaac gatcggggaa attcgagggg cccgtttcgt cctttaggga
    10981 ctcgtcagtg tactgatata agtacagacg ggcgtaataa cgcttacgta ggcgaggggt
    11041 tttacccctt ttgcctttgt aaccatcatc actttaaaag tacttactca ccccccaata
    11101 atctcttagc ctcgaggcct tttcagatca ttgagaattt taataaaata caaataaaat
    11161 aacaacacaa acaaagtccg ttcccctatg gtaagacaat gagtcggcca agcgccaatc
    11221 tcaaacagtc tatatacaga aacagataac aatatataat aaaatcaaaa agcaaacaaa
    11281 cgatcaatcc cgaaaggaac atcatctctt aaaaatcttt ttggtgtccc aaattctctg
    11341 tcctctttga atttcttctt agaggtcttc gaattatgag actttttagc tgtcgcagac
    11401 atgttttcag cttgatttcg tcgaatttgt ttccaaattt ctcgatttct tgtttcaaca
    11461 cgtttacgac acttttcaag gtgactacgg ccacaacaat tatacacatc aaaagtaaaa
    11521 tcattgataa caacacagac aaaccatcca caattatttt ccgcacacct acgtgtgaca
    11581 ccaaccatgt cagcaacttg ctttcgcaat ttgttagcat gaccgatact acacgtctcg
    11641 tgaccaagaa cagtgacttc attcacacaa cccttgagta cacacgtcat attgacaata
    11701 aatttcttta tgcattaaga cgagtttttc ttattagacg ctctcttaac atcttcactt
    11761 gacaataatg acttaggtct agtagtagct ggtctgtaag gaccatcgta cttcgcctca
    11821 atcgtcttca tctccacttg tttcaaagac atgaactcac cgaaaacctc ttgatcggta
    11881 aaatcgcctt caatgtaatc cgaactgttc agttcttcca cattgaggcg cctagtcgaa
    11941 tcagtagcaa cccatattag cgacacttcg atactgaaag ggtatgtacc gtcacagacc
    12001 ggaacgccct cagaaataca acccaccttc cagacatcaa gtacgttctc ctgtgtggaa
    12061 atactgtatt caaccttgta ctgcgcaact gaacagttat cgcaagctcg agctttgaat
    12121 ctctgaatag tctttcctct ctttcttttc tcagtaagtc tcgaatccaa cagatcgatt
    12181 tgaatgaacc caggcgtatc tgcaggtaga tgtgatgtta caaccaattg tacacctact
    12241 atctttagaa acgcgtaaga tttaatcatt ttgtaatccc tttggtcaaa aacgtctaac
    12301 ttgtgttcac tcttcttccc accaacatga tttaggacag tttctcttct tctgtgaaaa
    12361 tacttcggcc tcttcctcct accgtccaca aacaattcaa tggcccctag atttgagaat
    12421 tttgagactt cgaaagtctt cttcttcaag gtgaccaatg acttgtcttc catattacca
    12481 gtcgtaaaag tttgacacag ctctctgtgc cttcttccaa tcaaccttag ccgggttcaa
    12541 ttccttatcg ttttcgtctc ggaataatgt acacagagct gtaaaactct taatatgctt
    12601 ccatagcgca caaagcgcat gaacagaatc acctttgtac aaataccggt ctgaaacaga
    12661 ctcggacagt ttggatacca ccatgtagtt cccaagagct ctattggaat cattcagcga
    12721 gatgtagatc tcggctaaat gttgcacatc ctttatactc tttttcccca acttcgtcag
    12781 aacttttacc ggatctggca cgaactcgta acacgatgac gtcttaagca agaacttccc
    12841 acaaaacatt gggacatcgt acttaaaaat cttgcactcg aaattccact tagtagccaa
    12901 ctttggacac ggatcaacaa actgcgttcc tctaggaaac gcaatcagtg agtcatctcc
    12961 tccatatgta accatgactg ctttctccaa tggtaattca gacaagagtg cacacagtgt
    13021 tctatctgaa tttgcattat aagtatcagc atctccactc ttttgttggt accaaatatg
    13081 cgccatcata ccattttgaa tatctctcac agtagtttga gtgtgcgaca cctcccaaag
    13141 gaaggccgcc cactcatcta gcccaaataa cctgtaaatc tccagctgca gttgtaaatg
    13201 gaagcgattt gccgacttgt cgaatttaga catgtctatc tcaacaaagt cgtaagccgc
    13261 ttccgtattt aagaacttca ctcgatcgtt taaatcactc gatgtcattc tcgtgttgaa
    13321 cacaaaatgt ggttgcatag catccaactt gcgttcatta atttctttga aaattggacc
    13381 gaacaacgag ttgatcaact tctcgtgata cacaacagtc tgtagagctg agtattcaaa
    13441 ttgcggtgtt aaatcagtct taggtttcac gtcagatttg atcatataca tgtaagacga
    13501 atagtctatt tcatgtaact ctctcacatc tgctactacc ttagcctgta ccgctgtgct
    13561 ttgatttctc caccatctct ccatttgagc tctattgaca ataggatcag cccgaatttt
    13621 tcccacatcg tagacaacag atttcagctt cttcatcgtc tcttcgatta gaactgtcgc
    13681 gtgcacattt tcttgtagat cgggtgccgc ttggttcctt ttctgcaatg ctaacaaact
    13741 ctccaactga gtagtcttcc ttttgtcagg catagcagta cgaataaccg gtttcagaaa
    13801 cgtttctttt tcagcaaact tgtctttcca gtttccactt ttgatcgtaa cattgtctaa
    13861 tcgcaaattg caatcagtcg ttgccaccaa atacccgtct aggcttgagt ctcttaacga
    13921 atttcccgga aacaaagcgt cgtaccacat ctccaagtcc gtaatcgtac ctgaatctgg
    13981 aacggcgcaa ggcccttcat gatgtctaaa gacatcaaac ctagaccgaa accgtcataa
    14041 gaccgtctca gtaacaaaaa atcttgccaa agccgccttc gtaagcgcgg cactttccct
    14101 gatctctttg cttacatcgt cctctttcac agtttcatac acaagtgatt gtgtgtgacg
    14161 cgacaacgcc acgattaagt actcccgacc tctagcgatt gagtcatccg taggtttcgt
    14221 cctgactagg actacatctt tgaatgtctc tccttgcgac tcgtgtactg tactcacttt
    14281 agctgccact ttcctcaatg ccaacaaatt taccaactcc ttcttctcag actgaaggaa
    14341 agttatgtac tgagcaccag gcttcaaaga aacctgttct ttagacacaa tcttcctcac
    14401 cgtcatcgaa ttagcagtcg cgttatgtgt tctgacatct ccagtatagt acttgttcaa
    14461 tacggcagcc acatctgctg gacttctgta agtttctctt ttttctgtaa caatgtcaaa
    14521 ctttccgacc agactagaaa acctcaaatc aacttgagat accctaggct tgaaagaaat
    14581 ttgattctga tctccttgac agatacatcg tttagcacca gctatctgag cgcaaaagta
    14641 caccatacca gcatgggcca tgagagcttc atcgaaatgc aacacgtctc cggttaaaga
    14701 accatcaaca caatgcatca aaaaagaatc aaccgtcttc actctccttt tcaatttgca
    14761 tggaaaacct ttgctcgcga atctctcgat caagtcgtcg gttgctgctc tcccagtaga
    14821 gagaaccaca tcgacacaag gattagctga gttgacaatc attgtcgact taccacaacc
    14881 aggtacaccg tcaactaatt caaaagaaca gttaactggt cctagatcca gggcacttaa
    14941 attgtccaag atcaacctgt tattgcaaag atacgtctga tcgcaaacta tgacataatc
    15001 actcatcctt tcgaccgcga agtcgttgac caacatacct cgttcccaaa cagccacttc
    15061 taagtatccc gaccaatcat ccatacgttt gtctggttgg aacacataag catgttcagt
    15121 gaccactgga cgtatgcacc agttcttcat ctccacatcc cacactccca gtccgtgcgg
    15181 gtaattctga cctccagttc gaataactcc tctcgaatag tcacgataca gagacataat
    15241 ttcatcattt tgagcgactc tctccgcatc taagtagtac agaaactctc ttctagcgtc
    15301 cacagaatcg tctccactga caaccggcct ttttggtaat ctctcacctc ccatcacttg
    15361 gaagtaatcg acacgtttga ccgtttttcc tttgctgacc aaaggtttga cagctggcaa
    15421 cacgttctca aatcctggtg gcgttaaagg tttacacaac acctttgtac cgcttgtttc
    15481 ccctctttca cattcagctg aaaaggctga aacaacctcg tcaatttctg cttcagtgtc
    15541 aataagacca ctcagaaact gctccatgat ctcctcatcg tctacctttc ttgaaactcc
    15601 aactctagct tgggtcagca cgttactgtc ttccaacaac agacccagtc cgcttcgaga
    15661 tgaaccgacc accgatggtg gagaagttct caccgaactg ccagaatagt tagagaattt
    15721 tgcacttaga aactcgtcag cgatttcttt cttctgctgc aatagcttat cagacttggc
    15781 ttttacctcg gataaatctg gtaaaaccgt ctcaccggac tcagatgccg aatacagact
    15841 gctaaagaaa tcgttgattt cataatagcg gcacggattc tcaacgtcta agacatgggc
    15901 cccccgtttg atatcaaaca tatcacatag ttttttcacc agtccatctc ggatgtctcg
    15961 acgcataagc gtgattcctc caatggcata cacaacactc agaaatacta tcaccttggt
    16021 tttgtgcgag ttctcgtaac atagttctgg accatttggt gtgtccttcg cccaggttag
    16081 taccttcttt ggatagaatc caattagagt accaaccatg ccaacgaatc cgccgactgc
    16141 gatcttgtta gcaaactgct tccacagttt tcttgatatt aaacccagca catcagtcaa
    16201 tgacgtgata ggtttcgtct cagtcagaat tgcttcacgc aactccctca tgacaggtcg
    16261 cgccacttgt tccttcacta gcaaagtttg agccaacaac tgtaaatctc gagaatctac
    16321 actttgcttg ttcttcacgg ccgccccgtt tatgaataag acccaattat ttgaactcaa
    16381 gtatgattta acattgctta tggtcagctg ctggtcagat aacctagcta tgtaatccaa
    16441 acacttgtcc atgaattgtt tctctacaaa caggtctttc ttgaccaact ctcgcgttga
    16501 ttcgaccaga tcgaaaattg ggacaacaac catgttttcc catctactga tatatattct
    16561 tcggtagtac tcttgcgatg atagcgacct cctcggaact ccagctatcc tgtagatcga
    16621 aaaaagcatt gtgtctcctc tcacctgcca cggttcgaag tagaacacat tcccttggta
    16681 actcactggc ttccccagta gatacttctt gtactcttcc cagtcatgaa tgtaagaaaa
    16741 ggaaggatct ttctcaaaac cgaaatagat cttcccgttc ttcttcatgt agtaaccgtc
    16801 aacagaaggt aatggacctt cgtccactaa catactttca ggagcaaaaa gcatagcagc
    16861 atgaaccact ttagttttct tctcaaccat agtagccatc aagtctttca acttgaagtc
    16921 atacaggctg tgaagagcta tcgcatactg cgcgcctttc ttcttaccat cagaaaaacc
    16981 cctacatgta caaccctgat atgtattatt gcacctaacc gcgttaggcg cagcaatatt
    17041 ttcacgaaat tggtcataca tccacagctc attctccgac atctgtctct taccacttct
    17101 agcttgatca ccaatagcag taagaaatat ctgtcgagaa atttctctct gatcatcccg
    17161 aatatctaga tttggacaac aggaatgaat ccttttctca cctctagtta cccaaaacga
    17221 ccattgacca ccgatgtcat ccacctcttg cagagcagga actctatcta aaagcaataa
    17281 cgcttgtagt tttcgctcag cagccgcaaa cccatgaacc atgtttttgt cgtctttaaa
    17341 cacgatattg aattcaggat aaatttccat caacttgttc tgttccttct gagaaagaac
    17401 ttcatgaaca atcactggat ctttagtctt tctttctcta atcatgtcat cgactttatc
    17461 aacaacaact ctttgcatca tttgcccgat ctcacaatca ggtttagtca gcatcaagtc
    17521 aaagaaatga ctcctctgct cagcagacat ctcgtccaca ttgagcaatt gagacaactt
    17581 gaagttaccg ttcgccattg tatctgtatg tatttttaca aagtatcaaa gaaaaggttt
    17641 taggcacacc agtcactttc ccccactcgg tactaagctg gatagattaa accaaaaaga
    17701 aaaacaaaat aaaactgtag gtgataagta cgttctaccg cgttctcacc aaaatccaat
    17761 tagcacgttc taccgcgttc aaaggattga aatgttttat cctctccaaa tgaaatgaac
    17821 ttccttatat agaggaaggg tcttgcgaag gatagtggga ttgtgcgtca tcccttacgt
    17881 cagtggagat atcacatcaa tccacttgct ttgaagacgt ggttggaacg tcttcttttt
    17941 ccacgatgtt cctcgtgggt gggggtccat ctttgggacc actgtcggta gaggcatctt
    18001 gaacgatagc ctttccttta tcgcaatgat ggcatttgta gaagccatct tccttttcta
    18061 ctgtcctttc gatgaagtga cagatagctg ggcaatggaa tccgaggagg tttcccgata
    18121 ttaccctttg ttgaaaagtc tcaatagccc tctggtcttc tgagactgta tctttgatat
    18181 tcttggagta gacgagagtg tcgtgctcca ccatgttgac catcacatca atccacttgc
    18241 tttgaagacg tggttggaac gtcttctttt tccacgatgt tcctcgtggg tgggggtcca
    18301 tctttgggac cactgtcggt agaggcatct tgaacgatag cctttccttt atcgcaatga
    18361 tggcatttgt agaagccatc ttccttttct actgtccttt cgatgaagtg acagatagct
    18421 gggcaatgga atccgaggag gtttcccgat attacccttt gttgaaaagt ctcaatagcc
    18481 ctctggtctt ctgagactgt atctttgata ttcttggagt agacgagagt gtcgtgctcc
    18541 accatgttga cctgcaggca tgcgcaaatt taaagcgctg atatcgatcg cgcgcagatc
    18601 tgtcatgatg atcattgcaa ttggatccat ctccactgac gtaagggatg acgcacaatc
    18661 ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt tggagaggat
    18721 aaaacattgc acctatggtg ttgccctggc tggggtatgt cagtgatcgc agtagaatgt
    18781 actaattgac aagttggaga atacggtaga acgtccttat ccaacacagc ctttatccct
    18841 ctccctgacg aggtttttgt cagtgtaata tttctttttg aactatccag cttagtaccg
    18901 tacgggaaag tgactggtgt gcttatcttt gaaatgttac tttgggtttc ggttctttag
    18961 gttagtaaga aagcacttgt cttctcatac aaaggaaaac ctgagacgta tcgcttacga
    19021 aagtagcaat gaaagaaagg tggtggtttt aatcgctacc gcaaaaacga tggggtcgtt
    19081 ttaattaact tctcctacgc aagcgtctaa acggacgttg gggttttgct agtttcttta
    19141 gagaaaacta gctaagtctt taatgttatc attagagatg gcataaatat aatacttgtg
    19201 tctgctgata agatcatttt aatttggacg attagacttg ttgaactaca ggttactgaa
    19261 tcacttgcgc taatcaacat gggagatatg tacgatgaat catttgacaa gtcgggcggt
    19321 cctgctgact tgatggacga ttcttgggtg gaatcagttt cgtggaaaga tctgttgaag
    19381 aagttacaca gcataaaatt tgcactacag tctggtagag atgagatcac tgggttacta
    19441 gcggcactga atagacagtg tccttattca ccatatgagc agtttccaga taagaaggtg
    19501 tatttccttt tagactcacg ggctaacagt gctcttggtg tgattcagaa cgcttcagcg
    19561 ttcaagagac gagctgatga gaagaatgca gtggcgggtg ttacaaatat tcctgcgaat
    19621 ccaaacacaa cggttacgac gaaccaaggg agtactacta ctaccaaggc gaacactggc
    19681 tcgactttgg aagaagactt gtacacttat tacaaattcg atgatgcctc tacagctttc
    19741 cacaaatctc taacttcgtt agagaacatg gagttgaaga gttattaccg aaggaacttt
    19801 gagaaagtat tcgggattaa gtttggtgga gcagctgcta gttcatctgc accgcctcca
    19861 gcgagtggag gtccgatacg tcctaatccc aagctttagg gatttaagga cgtgaactct
    19921 gttgagatct ctgtgaaatt cagagggtgg gtgataccat attcactgat gccattagcg
    19981 acatctaaat agggctaatt gtgactaatt tgagggaatt tcctttacca ttgacgtcag
    20041 tgtcgttggt agcatttgag tttcgcaatg cacgaattac ttaggaagtg gcttgacgac
    20101 actaatgtgt tattgttaga taatggtttg gtggtcaagg tacgtagtag agtcccacat
    20161 attcgcacgt atgaagtaat tggaaagttg tcagtttttg ataattcact gggagatgat
//
