LOCUS       sequence-476825-       20348 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This all-in-one vector is used to specifically silence the GoPGF and
            HD1 gene via virus-induced gene silencing (VIGS), simultaneously..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 20348)
  AUTHORS   Hao Y, Yuan Z, Li Y, Zuo D, Cheng H, Wang Q, Zhang Y, Lv L, Liu J, 
            Song G
  TITLE     An all-in-one plant virus-based vector toolkit for streamlined gene 
            silencing, overexpression, and genome editing.
  JOURNAL   Plant Commun. 2025 May 19:101379. doi: 10.1016/j.xplc.2025.101379.
  PUBMED    40394900
REFERENCE   2  (bases 1 to 20348)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Commun. 2025 May 19:101379. doi: 10.1016/j.xplc.2025.101379."
COMMENT     Sequence Label: pVM2-GoPGF-HD1
FEATURES             Location/Qualifiers
     source          1..20348
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1213..1230
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      1265..1517
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(1526..1542)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1526..1542)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     misc_feature    1563..1587
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             2052..2702
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     primer_bind     complement(2890..2907)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(3141..3160)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     oriT            3383..3492
                     /note="incP origin of transfer"
     CDS             3525..3896
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     rep_origin      4023..4734
                     /label=oriV
                     /note="incP origin of replication"
     mobile_element  5357..6124
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     CDS             6316..7110
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     CDS             7409..8557
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKTYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    10153..10177
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     primer_bind     complement(10424..10445)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     10636..10655
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10780..10802
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     10794..10811
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     10795..10811
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(10820..11072)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        complement(17926..18271)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     promoter        18801..18847
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
ORIGIN
        1 acgctgtttg agggaaaagt agagaacgta tttgttttta tgttcaggcg gttcttgtgt
       61 gtcaacaaag atggacattg ttactcaagg aagcacgatg agctttatta ttacggacga
      121 gtggacttag attctgtgag taaggttacc gaattggcca gttaacgcta gcgaattgac
      181 gaacggccta aacctgtagt ttctttcttg ggctcgactt gttgtttgtc ttcaagaatc
      241 cgctcctttt gatcacctga aaccattcca aaatcaacaa aaggaatact tggggttgag
      301 atttggggtt gggaatcatg ggcaggttga tttgaaccca ggctgttgtt gatcccagaa
      361 ctgaatatcg atttagtgag ttgaagggtg ccccagtctt ccatgatgag acctagggga
      421 attggaccaa agcctttggg gttgaggtct gaagcttcaa gagaatctgc agttgtcatc
      481 atgaatcatg tcaacttagt tgagattctc atggatgtga atcaatggtc aagtgtgttt
      541 tgcggtattg tttcaagggc tatgacttta gaagtcctat caactggagt tgcaggaaac
      601 tacaatgggg ccttgcaagt gatgacggct gagttccaag tcccttcacc acttgtacca
      661 actcgggaaa attatttcgc gaggtactgt aagcagcata ttgatggaac ttgggcaggt
      721 ctcactagtc tgcaggcatg ccattcgagg cccgggcatg tcccgaagac attaaactac
      781 ggttctttaa gtagatccgt gtctgaagtt ttaggttcaa tttaaaccta cgagattgac
      841 attctcgact gatcttgatt gatcggtaag tcttttgtaa tttaattttc tttttgattt
      901 tattttaaat tgttatctgt ttctgtgtat agactgtttg agatcggcgt ttggccgact
      961 cattgtctta ccatagggga acggactttg tttgtgttgt tattttattt gtattttatt
     1021 aaaattctca acgatctgaa aaagcctcgc ggctaagaga ttgttggggg gtgagtaagt
     1081 acttttaaag tgatgatggt tacaaaggca aaaggggtaa aacccctcgc ctacgtaagc
     1141 gttattacgc ccgtctgtac ttatatcagt acactgacga gtccctaaag gacgaaacgg
     1201 gagaacgcta gccaccacca ccaccaccac gtgtgaatta caggtgacca gctcgaattt
     1261 ccccgatcgt tcaaacattt ggcaataaag tttcttaaga ttgaatcctg ttgccggtct
     1321 tgcgatgatt atcatataat ttctgttgaa ttacgttaag catgtaataa ttaacatgta
     1381 atgcatgacg ttatttatga gatgggtttt tatgattaga gtcccgcaat tatacattta
     1441 atacgcgata gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc gcgcggtgtc
     1501 atctatgtta ctagatcggg aattggtcat agctgtttcc tgggatccaa actatcagtg
     1561 tttgacagga tatattggcg ggtaaaccta agagaaaaga gcgtttatta gaataatcgg
     1621 atatttaaaa gggcgtgaaa aggtttatcc gttcgtccat ttgtatgtgc atgccaacca
     1681 cagggttccc cagatctggc gccggccagc gagacgagca agattggccg ccgcccgaaa
     1741 cgatccgaca gcgcgcccag cacaggtgcg caggcaaatt gcaccaacgc atacagcgcc
     1801 agcagaatgc catagtgggc ggtgacgtcg ttcgagtgaa ccagatcgcg caggaggccc
     1861 ggcagcaccg gcataatcag gccgatgccg acagcgtcga gcgcgacagt gctcagaatt
     1921 acgatcaggg gtatgttggg tttcacgtct ggcctccgga ccagcctccg ctggtccgat
     1981 tgaacgcgcg gattctttat cactgataag ttggtggaca tattatgttt atcagtgata
     2041 aagtgtcaag catgacaaag ttgcagccga atacagtgat ccgtgccgcc ctggacctgt
     2101 tgaacgaggt cggcgtagac ggtctgacga cacgcaaact ggcggaacgg ttgggggttc
     2161 agcagccggc gctttactgg cacttcagga acaagcgggc gctgctcgac gcactggccg
     2221 aagccatgct ggcggagaat catacgcatt cggtgccgag agccgacgac gactggcgct
     2281 catttctgat cgggaatgcc cgcagcttca ggcaggcgct gctcgcctac cgcgatggcg
     2341 cgcgcatcca tgccggcacg cgaccgggcg caccgcagat ggaaacggcc gacgcgcagc
     2401 ttcgcttcct ctgcgaggcg ggtttttcgg ccggggacgc cgtcaatgcg ctgatgacaa
     2461 tcagctactt cactgttggg gccgtgcttg aggagcaggc cggcgacagc gatgccggcg
     2521 agcgcggcgg caccgttgaa caggctccgc tctcgccgct gttgcgggcc gcgatagacg
     2581 ccttcgacga agccggtccg gacgcagcgt tcgagcaggg actcgcggtg attgtcgatg
     2641 gattggcgaa aaggaggctc gttgtcagga acgttgaagg accgagaaag ggtgacgatt
     2701 gatcaggacc gctgccggag cgcaacccac tcactacagc agagccatgt agacaacatc
     2761 ccctccccct ttccaccgcg tcagacgccc gtagcagccc gctacgggct ttttcatgcc
     2821 ctgccctagc gtccaagcct cacggccgcg ctcggcctct ctggcggcct tctggcgctc
     2881 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     2941 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     3001 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     3061 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     3121 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     3181 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     3241 cgtggcgctt ttccgctgca taaccctgct tcggggtcat tatagcgatt ttttcggtat
     3301 atccatcctt tttcgcacga tatacaggat tttgccaaag ggttcgtgta gactttcctt
     3361 ggtgtatcca acggcgtcag ccgggcagga taggtgaagt aggcccaccc gcgagcgggt
     3421 gttccttctt cactgtccct tattcgcacc tggcggtgct caacgggaat cctgctctgc
     3481 gaggctggcc ggctaccgcc ggcgtaacag atgagggcaa gcggatggct gatgaaacca
     3541 agccaaccag gaagggcagc ccacctatca aggtgtactg ccttccagac gaacgaagag
     3601 cgattgagga aaaggcggcg gcggccggca tgagcctgtc ggcctacctg ctggccgtcg
     3661 gccagggcta caaaatcacg ggcgtcgtgg actatgagca cgtccgcgag ctggcccgca
     3721 tcaatggcga cctgggccgc ctgggcggcc tgctgaaact ctggctcacc gacgacccgc
     3781 gcacggcgcg gttcggtgat gccacgatcc tcgccctgct ggcgaagatc gaagagaagc
     3841 aggacgagct tggcaaggtc atgatgggcg tggtccgccc gagggcagag ccatgacttt
     3901 tttagccgct aaaacggccg gggggtgcgc gtgattgcca agcacgtccc catgcgctcc
     3961 atcaagaaga gcgacttcgc ggagctggtg aagtacatca ccgacgagca aggcaagacc
     4021 gagcgccttt ccgacgctca ccgggctggt tgccctcgcc gctgggctgg cggccgtcta
     4081 tggccctgca aacgcgccag aaacgccgtc gaagccgtgt gcgagacacc gcggccgccg
     4141 gcgttgtgga tacctcgcgg aaaacttggc cctcactgac agatgagggg cggacgttga
     4201 cacttgaggg gccgactcac ccggcgcggc gttgacagat gaggggcagg ctcgatttcg
     4261 gccggcgacg tggagctggc cagcctcgca aatcggcgaa aacgcctgat tttacgcgag
     4321 tttcccacag atgatgtgga caagcctggg gataagtgcc ctgcggtatt gacacttgag
     4381 gggcgcgact actgacagat gaggggcgcg atccttgaca cttgaggggc agagtgctga
     4441 cagatgaggg gcgcacctat tgacatttga ggggctgtcc acaggcagaa aatccagcat
     4501 ttgcaagggt ttccgcccgt ttttcggcca ccgctaacct gtcttttaac ctgcttttaa
     4561 accaatattt ataaaccttg tttttaacca gggctgcgcc ctgtgcgcgt gaccgcgcac
     4621 gccgaagggg ggtgcccccc cttctcgaac cctcccggcc cgctaacgcg ggcctcccat
     4681 ccccccaggg gctgcgcccc tcggccgcga acggcctcac cccaaaaatg gcagcgctgg
     4741 cagtccttgc cattgccggg atcggggcag taacgggatg ggcgatcagc ccgagcgcga
     4801 cgcccggaag cattgacgtg ccgcaggtgc tggcatcgac attcagcgac caggtgccgg
     4861 gcagtgaggg cggcggcctg ggtggcggcc tgcccttcac ttcggccgtc ggggcattca
     4921 cggacttcat ggcggggccg gcaattttta ccttgggcat tcttggcata gtggtcgcgg
     4981 gtgccgtgct cgtgttcggg ggtgcgataa acccagcgaa ccatttgagg tgataggtaa
     5041 gattataccg aggtatgaaa acgagaattg gacctttaca gaattactct atgaagcgcc
     5101 atatttaaaa agctaccaag acgaagagga tgaagaggat gaggaggcag attgccttga
     5161 atatattgac aatactgata agataatata tcttttatat agaagatatc gccgtatgta
     5221 aggatttcag ggggcaaggc ataggcagcg cgcttatcaa tatatctata gaatgggcaa
     5281 agcataaaaa cttgcatgga ctaatgcttg aaacccagga caataacctt atagcttgta
     5341 aattctatca taattgggta atgactccaa cttattgata gtgttttatg ttcagataat
     5401 gcccgatgac tttgtcatgc agctccaccg attttgagaa cgacagcgac ttccgtccca
     5461 gccgtgccag gtgctgcctc agattcaggt tatgccgctc aattcgctgc gtatatcgct
     5521 tgctgattac gtgcagcttt cccttcaggc gggattcata cagcggccag ccatccgtca
     5581 tccatatcac cacgtcaaag ggtgacagca ggctcataag acgccccagc gtcgccatag
     5641 tgcgttcacc gaatacgtgc gcaacaaccg tcttccggag actgtcatac gcgtaaaaca
     5701 gccagcgctg gcgcgattta gccccgacat agccccactg ttcgtccatt tccgcgcaga
     5761 cgatgacgtc actgcccggc tgtatgcgcg aggttaccga ctgcggcctg agttttttaa
     5821 gtgacgtaaa atcgtgttga ggccaacgcc cataatgcgg gctgttgccc ggcatccaac
     5881 gccattcatg gccatatcaa tgattttctg gtgcgtaccg ggttgagaag cggtgtaagt
     5941 gaactgcagt tgccatgttt tacggcagtg agagcagaga tagcgctgat gtccggcggt
     6001 gcttttgccg ttacgcacca ccccgtcagt agctgaacag gagggacagc tgatagaaac
     6061 agaagccact ggagcacctc aaaaacacca tcatacacta aatcagtaag ttggcagcat
     6121 cacccataat tgtggtttca aaatcggctc cgtcgatact atgttatacg ccaactttga
     6181 aaacaacttt gaaaaagctg ttttctggta tttaaggttt tagaatgcaa ggaacagtga
     6241 attggagttc gtcttgttat aattagcttc ttggggtatc tttaaatact gtagaaaaga
     6301 ggaaggaaat aataaatggc taaaatgaga atatcaccgg aattgaaaaa actgatcgaa
     6361 aaataccgct gcgtaaaaga tacggaagga atgtctcctg ctaaggtata taagctggtg
     6421 ggagaaaatg aaaacctata tttaaaaatg acggacagcc ggtataaagg gaccacctat
     6481 gatgtggaac gggaaaagga catgatgcta tggctggaag gaaagctgcc tgttccaaag
     6541 gtcctgcact ttgaacggca tgatggctgg agcaatctgc tcatgagtga ggccgatggc
     6601 gtcctttgct cggaagagta tgaagatgaa caaagccctg aaaagattat cgagctgtat
     6661 gcggagtgca tcaggctctt tcactccatc gacatatcgg attgtcccta tacgaatagc
     6721 ttagacagcc gcttagccga attggattac ttactgaata acgatctggc cgatgtggat
     6781 tgcgaaaact gggaagaaga cactccattt aaagatccgc gcgagctgta tgatttttta
     6841 aagacggaaa agcccgaaga ggaacttgtc ttttcccacg gcgacctggg agacagcaac
     6901 atctttgtga aagatggcaa agtaagtggc tttattgatc ttgggagaag cggcagggcg
     6961 gacaagtggt atgacattgc cttctgcgtc cggtcgatca gggaggatat cggggaagaa
     7021 cagtatgtcg agctattttt tgacttactg gggatcaagc ctgattggga gaaaataaaa
     7081 tattatattt tactggatga attgttttag tacctagatg tggcgcaacg atgccggcga
     7141 caagcaggag cgcaccgact tcttccgcat caagtgtttt ggctctcagg ccgaggccca
     7201 cggcaagtat ttgggcaagg ggtcgctggt attcgtgcag ggcaagattc ggaataccaa
     7261 gtacgagaag gacggccaga cggtctacgg gaccgacttc attgccgata aggtggatta
     7321 tctggacacc aaggcaccag gcgggtcaaa tcaggaataa gggcacattg ccccggcgtg
     7381 agtcggggca atcccgcaag gagggtgaat gaatcggacg tttgaccgga agacatacag
     7441 gcaagaactg atcgacgcgg ggttttccgc cgaggatgcc gaaaccatcg caagccgcac
     7501 cgtcatgcgt gcgccccgcg aaaccttcca gtccgtcggc tcgatggtcc agcaagctac
     7561 ggccaagatc gagcgcgaca gcgtgcaact ggctccccct gccctgcccg cgccatcggc
     7621 cgccgtggag cgttcgcgtc gtctcgaaca ggaggcggca ggtttggcga agtcgatgac
     7681 catcgacacg cgaggaacta tgacgaccaa gaagcgaaaa accgccggcg aggacctggc
     7741 aaaacaggtc agcgaggcca agcaggccgc gttgctgaaa cacacgaagc agcagatcaa
     7801 ggaaatgcag ctttccttgt tcgatattgc gccgtggccg gacacgatgc gagcgatgcc
     7861 aaacgacacg gcccgctctg ccctgttcac cacgcgcaac aagaaaatcc cgcgcgaggc
     7921 gctgcaaaac aaggtcattt tccacgtcaa caaggacgtg aagatcacct acaccggcgt
     7981 cgagctgcgg gccgacgatg acgaactggt gtggcagcag gtgttggagt acgcgaagcg
     8041 cacccctatc ggcgagccga tcaccttcac gttctacgag ctttgccagg acctgggctg
     8101 gtcgatcaat ggccggtatt acacgaaggc cgaggaatgc ctgtcgcgcc tacaggcgac
     8161 ggcgatgggc ttcacgtccg accgcgttgg gcacctggaa tcggtgtcgc tgctgcaccg
     8221 cttccgcgtc ctggaccgtg gcaagaaaac gtcccgttgc caggtcctga tcgacgagga
     8281 aatcgtcgtg ctgtttgctg gcgaccacta cacgaaattc atatgggaga agtaccgcaa
     8341 gctgtcgccg acggcccgac ggatgttcga ctatttcagc tcgcaccggg agccgtaccc
     8401 gctcaagctg gaaaccttcc gcctcatgtg cggatcggat tccacccgcg tgaagaagtg
     8461 gcgcgagcag gtcggcgaag cctgcgaaga gttgcgaggc agcggcctgg tggaacacgc
     8521 ctgggtcaat gatgacctgg tgcattgcaa acgctagggc cttgtggggt cagttccggc
     8581 tgggggttca gcagccagcg ctttactggc atttcaggaa caagcgggca ctgctcgacg
     8641 cacttgcttc gctcagtatc gctcgggacg cacggcgcgc tctacgaact gccgataaac
     8701 agaggattaa aattgacaat tgtgattaag gctcagattc gacggcttgg agcggccgac
     8761 gtgcaggatt tccgcgagat ccgattgtcg gccctgaaga aagctccaga gatgttcggg
     8821 tccgtttacg agcacgagga gaaaaagccc atggaggcgt tcgctgaacg gttgcgagat
     8881 gccgtggcat tcggcgccta catcgacggc gagatcattg ggctgtcggt cttcaaacag
     8941 gaggacggcc ccaaggacgc tcacaaggcg catctgtccg gcgttttcgt ggagcccgaa
     9001 cagcgaggcc gaggggtcgc cggtatgctg ctgcgggcgt tgccggcggg tttattgctc
     9061 gtgatgatcg tccgacagat tccaacggga atctggtgga tgcgcatctt catcctcggc
     9121 gcacttaata tttcgctatt ctggagcttg ttgtttattt cggtctaccg cctgccgggc
     9181 ggggtcgcgg cgacggtagg cgctgtgcag ccgctgatgg tcgtgttcat ctctgccgct
     9241 ctgctaggta gcccgatacg attgatggcg gtcctggggg ctatttgcgg aactgcgggc
     9301 gtggcgctgt tggtgttgac accaaacgca gcgctagatc ctgtcggcgt cgcagcgggc
     9361 ctggcggggg cggtttccat ggcgttcgga accgtgctga cccgcaagtg gcaacctccc
     9421 gtgcctctgc tcacctttac cgcctggcaa ctggcggccg gaggacttct gctcgttcca
     9481 gtagctttag tgtttgatcc gccaatcccg atgcctacag gaaccaatgt tctcggcctg
     9541 gcgtggctcg gcctgatcgg agcgggttta acctacttcc tttggttccg ggggatctcg
     9601 cgactcgaac ctacagttgt ttccttactg ggctttctca gccccagatc tggggtcgat
     9661 cagccgggga tgcatcaggc cgacagtcgg aacttcgggt ccccgacctg taccattcgg
     9721 tgagcaatgg ataggggagt tgatatcgtc aacgttcact tctaaagaaa tagcgccact
     9781 cagcttcctc agcggcttta tccagcgatt tcctattatg tcggcatagt tctcaagatc
     9841 gacagcctgt cacggttaag cgagaaatga ataagaaggc tgataattcg gatctctgcg
     9901 agggagatga tatttgatca caggcagcaa cgctctgtca tcgttacaat caacatgcta
     9961 ccctccgcga gatcatccgt gtttcaaacc cggcagctta gttgccgttc ttccgaatag
    10021 catcggtaac atgagcaaag tctgccgcct tacaacggct ctcccgctga cgccgtcccg
    10081 gactgatggg ctgcctgtat cgagtggtga ttttgtgccg agctgccggt cggggagctg
    10141 ttggctggct ggtggcagga tatattgtgg tgtaaacaaa ttgacgctta gacaacttaa
    10201 taacacattg cggacgtttt taatgtactg gggtggtttt tcttttcacc agtgagacgg
    10261 gcaacagctg attgcccttc accgcctggc cctgagagag ttgcagcaag cggtccacgc
    10321 tggtttgccc cagcaggcga aaatcctgtt tgatggtggt tccgaaatcg gcaaaatccc
    10381 ttataaatca aaagaatagc ccgagatagg gttgagtgtt gttccagttt ggaacaagag
    10441 tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga
    10501 tggcccacta cgtgaaccat cacccaaatc aagttttttg gggtcgaggt gccgtaaagc
    10561 actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa
    10621 cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gccattcagg ctgcgcaact
    10681 gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat
    10741 gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa
    10801 cgacggccag tgaattcccg atctagtaac atagatgaca ccgcgcgcga taatttatcc
    10861 tagtttgcgc gctatatttt gttttctatc gcgtattaaa tgtataattg cgggactcta
    10921 atcataaaaa cccatctcat aaataacgtc atgcattaca tgttaattat tacatgctta
    10981 acgtaattca acagaaatta tatgataatc atcgcaagac cggcaacagg attcaatctt
    11041 aagaaacttt attgccaaat gtttgaacga tcggggaaat tcgaggggcc cgtttcgtcc
    11101 tttagggact cgtcagtgta ctgatataag tacagacggg cgtaataacg cttacgtagg
    11161 cgaggggttt tacccctttt gcctttgtaa ccatcatcac tttaaaagta cttactcacc
    11221 ccccaataat ctcttagcct cgaggccttt tcagatcatt gagaatttta ataaaataca
    11281 aataaaataa caacacaaac aaagtccgtt cccctatggt aagacaatga gtcggccaag
    11341 cgccaatctc aaacagtcta tatacagaaa cagataacaa tatataataa aatcaaaaag
    11401 caaacaaacg atcaatcccg aaaggaacat catctcttaa aaatcttttt ggtgtcccaa
    11461 attctctgtc ctctttgaat ttcttcttag aggtcttcga attatgagac tttttagctg
    11521 tcgcagacat gttttcagct tgatttcgtc gaatttgttt ccaaatttct cgatttcttg
    11581 tttcaacacg tttacgacac ttttcaaggt gactacggcc acaacaatta tacacatcaa
    11641 aagtaaaatc attgataaca acacagacaa accatccaca attattttcc gcacacctac
    11701 gtgtgacacc aaccatgtca gcaacttgct ttcgcaattt gttagcatga ccgatactac
    11761 acgtctcgtg accaagaaca gtgacttcat tcacacaacc cttgagtaca cacgtcatat
    11821 tgacaataaa tttctttatg cattaagacg agtttttctt attagacgct ctcttaacat
    11881 cttcacttga caataatgac ttaggtctag tagtagctgg tctgtaagga ccatcgtact
    11941 tcgcctcaat cgtcttcatc tccacttgtt tcaaagacat gaactcaccg aaaacctctt
    12001 gatcggtaaa atcgccttca atgtaatccg aactgttcag ttcttccaca ttgaggcgcc
    12061 tagtcgaatc agtagcaacc catattagcg acacttcgat actgaaaggg tatgtaccgt
    12121 cacagaccgg aacgccctca gaaatacaac ccaccttcca gacatcaagt acgttctcct
    12181 gtgtggaaat actgtattca accttgtact gcgcaactga acagttatcg caagctcgag
    12241 ctttgaatct ctgaatagtc tttcctctct ttcttttctc agtaagtctc gaatccaaca
    12301 gatcgatttg aatgaaccca ggcgtatctg caggtagatg tgatgttaca accaattgta
    12361 cacctactat ctttagaaac gcgtaagatt taatcatttt gtaatccctt tggtcaaaaa
    12421 cgtctaactt gtgttcactc ttcttcccac caacatgatt taggacagtt tctcttcttc
    12481 tgtgaaaata cttcggcctc ttcctcctac cgtccacaaa caattcaatg gcccctagat
    12541 ttgagaattt tgagacttcg aaagtcttct tcttcaaggt gaccaatgac ttgtcttcca
    12601 tattaccagt cgtaaaagtt tgacacagct ctctgtgcct tcttccaatc aaccttagcc
    12661 gggttcaatt ccttatcgtt ttcgtctcgg aataatgtac acagagctgt aaaactctta
    12721 atatgcttcc atagcgcaca aagcgcatga acagaatcac ctttgtacaa ataccggtct
    12781 gaaacagact cggacagttt ggataccacc atgtagttcc caagagctct attggaatca
    12841 ttcagcgaga tgtagatctc ggctaaatgt tgcacatcct ttatactctt tttccccaac
    12901 ttcgtcagaa cttttaccgg atctggcacg aactcgtaac acgatgacgt cttaagcaag
    12961 aacttcccac aaaacattgg gacatcgtac ttaaaaatct tgcactcgaa attccactta
    13021 gtagccaact ttggacacgg atcaacaaac tgcgttcctc taggaaacgc aatcagtgag
    13081 tcatctcctc catatgtaac catgactgct ttctccaatg gtaattcaga caagagtgca
    13141 cacagtgttc tatctgaatt tgcattataa gtatcagcat ctccactctt ttgttggtac
    13201 caaatatgcg ccatcatacc attttgaata tctctcacag tagtttgagt gtgcgacacc
    13261 tcccaaagga aggccgccca ctcatctagc ccaaataacc tgtaaatctc cagctgcagt
    13321 tgtaaatgga agcgatttgc cgacttgtcg aatttagaca tgtctatctc aacaaagtcg
    13381 taagccgctt ccgtatttaa gaacttcact cgatcgttta aatcactcga tgtcattctc
    13441 gtgttgaaca caaaatgtgg ttgcatagca tccaacttgc gttcattaat ttctttgaaa
    13501 attggaccga acaacgagtt gatcaacttc tcgtgataca caacagtctg tagagctgag
    13561 tattcaaatt gcggtgttaa atcagtctta ggtttcacgt cagatttgat catatacatg
    13621 taagacgaat agtctatttc atgtaactct ctcacatctg ctactacctt agcctgtacc
    13681 gctgtgcttt gatttctcca ccatctctcc atttgagctc tattgacaat aggatcagcc
    13741 cgaatttttc ccacatcgta gacaacagat ttcagcttct tcatcgtctc ttcgattaga
    13801 actgtcgcgt gcacattttc ttgtagatcg ggtgccgctt ggttcctttt ctgcaatgct
    13861 aacaaactct ccaactgagt agtcttcctt ttgtcaggca tagcagtacg aataaccggt
    13921 ttcagaaacg tttctttttc agcaaacttg tctttccagt ttccactttt gatcgtaaca
    13981 ttgtctaatc gcaaattgca atcagtcgtt gccaccaaat acccgtctag gcttgagtct
    14041 cttaacgaat ttcccggaaa caaagcgtcg taccacatct ccaagtccgt aatcgtacct
    14101 gaatctggaa cggcgcaagg cccttcatga tgtctaaaga catcaaacct agaccgaaac
    14161 cgtcataaga ccgtctcagt aacaaaaaat cttgccaaag ccgccttcgt aagcgcggca
    14221 ctttccctga tctctttgct tacatcgtcc tctttcacag tttcatacac aagtgattgt
    14281 gtgtgacgcg acaacgccac gattaagtac tcccgacctc tagcgattga gtcatccgta
    14341 ggtttcgtcc tgactaggac tacatctttg aatgtctctc cttgcgactc gtgtactgta
    14401 ctcactttag ctgccacttt cctcaatgcc aacaaattta ccaactcctt cttctcagac
    14461 tgaaggaaag ttatgtactg agcaccaggc ttcaaagaaa cctgttcttt agacacaatc
    14521 ttcctcaccg tcatcgaatt agcagtcgcg ttatgtgttc tgacatctcc agtatagtac
    14581 ttgttcaata cggcagccac atctgctgga cttctgtaag tttctctttt ttctgtaaca
    14641 atgtcaaact ttccgaccag actagaaaac ctcaaatcaa cttgagatac cctaggcttg
    14701 aaagaaattt gattctgatc tccttgacag atacatcgtt tagcaccagc tatctgagcg
    14761 caaaagtaca ccataccagc atgggccatg agagcttcat cgaaatgcaa cacgtctccg
    14821 gttaaagaac catcaacaca atgcatcaaa aaagaatcaa ccgtcttcac tctccttttc
    14881 aatttgcatg gaaaaccttt gctcgcgaat ctctcgatca agtcgtcggt tgctgctctc
    14941 ccagtagaga gaaccacatc gacacaagga ttagctgagt tgacaatcat tgtcgactta
    15001 ccacaaccag gtacaccgtc aactaattca aaagaacagt taactggtcc tagatccagg
    15061 gcacttaaat tgtccaagat caacctgtta ttgcaaagat acgtctgatc gcaaactatg
    15121 acataatcac tcatcctttc gaccgcgaag tcgttgacca acatacctcg ttcccaaaca
    15181 gccacttcta agtatcccga ccaatcatcc atacgtttgt ctggttggaa cacataagca
    15241 tgttcagtga ccactggacg tatgcaccag ttcttcatct ccacatccca cactcccagt
    15301 ccgtgcgggt aattctgacc tccagttcga ataactcctc tcgaatagtc acgatacaga
    15361 gacataattt catcattttg agcgactctc tccgcatcta agtagtacag aaactctctt
    15421 ctagcgtcca cagaatcgtc tccactgaca accggccttt ttggtaatct ctcacctccc
    15481 atcacttgga agtaatcgac acgtttgacc gtttttcctt tgctgaccaa aggtttgaca
    15541 gctggcaaca cgttctcaaa tcctggtggc gttaaaggtt tacacaacac ctttgtaccg
    15601 cttgtttccc ctctttcaca ttcagctgaa aaggctgaaa caacctcgtc aatttctgct
    15661 tcagtgtcaa taagaccact cagaaactgc tccatgatct cctcatcgtc tacctttctt
    15721 gaaactccaa ctctagcttg ggtcagcacg ttactgtctt ccaacaacag acccagtccg
    15781 cttcgagatg aaccgaccac cgatggtgga gaagttctca ccgaactgcc agaatagtta
    15841 gagaattttg cacttagaaa ctcgtcagcg atttctttct tctgctgcaa tagcttatca
    15901 gacttggctt ttacctcgga taaatctggt aaaaccgtct caccggactc agatgccgaa
    15961 tacagactgc taaagaaatc gttgatttca taatagcggc acggattctc aacgtctaag
    16021 acatgggccc cccgtttgat atcaaacata tcacatagtt ttttcaccag tccatctcgg
    16081 atgtctcgac gcataagcgt gattcctcca atggcataca caacactcag aaatactatc
    16141 accttggttt tgtgcgagtt ctcgtaacat agttctggac catttggtgt gtccttcgcc
    16201 caggttagta ccttctttgg atagaatcca attagagtac caaccatgcc aacgaatccg
    16261 ccgactgcga tcttgttagc aaactgcttc cacagttttc ttgatattaa acccagcaca
    16321 tcagtcaatg acgtgatagg tttcgtctca gtcagaattg cttcacgcaa ctccctcatg
    16381 acaggtcgcg ccacttgttc cttcactagc aaagtttgag ccaacaactg taaatctcga
    16441 gaatctacac tttgcttgtt cttcacggcc gccccgttta tgaataagac ccaattattt
    16501 gaactcaagt atgatttaac attgcttatg gtcagctgct ggtcagataa cctagctatg
    16561 taatccaaac acttgtccat gaattgtttc tctacaaaca ggtctttctt gaccaactct
    16621 cgcgttgatt cgaccagatc gaaaattggg acaacaacca tgttttccca tctactgata
    16681 tatattcttc ggtagtactc ttgcgatgat agcgacctcc tcggaactcc agctatcctg
    16741 tagatcgaaa aaagcattgt gtctcctctc acctgccacg gttcgaagta gaacacattc
    16801 ccttggtaac tcactggctt ccccagtaga tacttcttgt actcttccca gtcatgaatg
    16861 taagaaaagg aaggatcttt ctcaaaaccg aaatagatct tcccgttctt cttcatgtag
    16921 taaccgtcaa cagaaggtaa tggaccttcg tccactaaca tactttcagg agcaaaaagc
    16981 atagcagcat gaaccacttt agttttcttc tcaaccatag tagccatcaa gtctttcaac
    17041 ttgaagtcat acaggctgtg aagagctatc gcatactgcg cgcctttctt cttaccatca
    17101 gaaaaacccc tacatgtaca accctgatat gtattattgc acctaaccgc gttaggcgca
    17161 gcaatatttt cacgaaattg gtcatacatc cacagctcat tctccgacat ctgtctctta
    17221 ccacttctag cttgatcacc aatagcagta agaaatatct gtcgagaaat ttctctctga
    17281 tcatcccgaa tatctagatt tggacaacag gaatgaatcc ttttctcacc tctagttacc
    17341 caaaacgacc attgaccacc gatgtcatcc acctcttgca gagcaggaac tctatctaaa
    17401 agcaataacg cttgtagttt tcgctcagca gccgcaaacc catgaaccat gtttttgtcg
    17461 tctttaaaca cgatattgaa ttcaggataa atttccatca acttgttctg ttccttctga
    17521 gaaagaactt catgaacaat cactggatct ttagtctttc tttctctaat catgtcatcg
    17581 actttatcaa caacaactct ttgcatcatt tgcccgatct cacaatcagg tttagtcagc
    17641 atcaagtcaa agaaatgact cctctgctca gcagacatct cgtccacatt gagcaattga
    17701 gacaacttga agttaccgtt cgccattgta tctgtatgta tttttacaaa gtatcaaaga
    17761 aaaggtttta ggcacaccag tcactttccc ccactcggta ctaagctgga tagattaaac
    17821 caaaaagaaa aacaaaataa aactgtaggt gataagtacg ttctaccgcg ttctcaccaa
    17881 aatccaatta gcacgttcta ccgcgttcaa aggattgaaa tgttttatcc tctccaaatg
    17941 aaatgaactt ccttatatag aggaagggtc ttgcgaagga tagtgggatt gtgcgtcatc
    18001 ccttacgtca gtggagatat cacatcaatc cacttgcttt gaagacgtgg ttggaacgtc
    18061 ttctttttcc acgatgttcc tcgtgggtgg gggtccatct ttgggaccac tgtcggtaga
    18121 ggcatcttga acgatagcct ttcctttatc gcaatgatgg catttgtaga agccatcttc
    18181 cttttctact gtcctttcga tgaagtgaca gatagctggg caatggaatc cgaggaggtt
    18241 tcccgatatt accctttgtt gaaaagtctc aatagccctc tggtcttctg agactgtatc
    18301 tttgatattc ttggagtaga cgagagtgtc gtgctccacc atgttgacca tcacatcaat
    18361 ccacttgctt tgaagacgtg gttggaacgt cttctttttc cacgatgttc ctcgtgggtg
    18421 ggggtccatc tttgggacca ctgtcggtag aggcatcttg aacgatagcc tttcctttat
    18481 cgcaatgatg gcatttgtag aagccatctt ccttttctac tgtcctttcg atgaagtgac
    18541 agatagctgg gcaatggaat ccgaggaggt ttcccgatat taccctttgt tgaaaagtct
    18601 caatagccct ctggtcttct gagactgtat ctttgatatt cttggagtag acgagagtgt
    18661 cgtgctccac catgttgacc tgcaggcatg cgcaaattta aagcgctgat atcgatcgcg
    18721 cgcagatctg tcatgatgat cattgcaatt ggatccatct ccactgacgt aagggatgac
    18781 gcacaatccc actatccttc gcaagaccct tcctctatat aaggaagttc atttcatttg
    18841 gagaggataa aacattgcac ctatggtgtt gccctggctg gggtatgtca gtgatcgcag
    18901 tagaatgtac taattgacaa gttggagaat acggtagaac gtccttatcc aacacagcct
    18961 ttatccctct ccctgacgag gtttttgtca gtgtaatatt tctttttgaa ctatccagct
    19021 tagtaccgta cgggaaagtg actggtgtgc ttatctttga aatgttactt tgggtttcgg
    19081 ttctttaggt tagtaagaaa gcacttgtct tctcatacaa aggaaaacct gagacgtatc
    19141 gcttacgaaa gtagcaatga aagaaaggtg gtggttttaa tcgctaccgc aaaaacgatg
    19201 gggtcgtttt aattaacttc tcctacgcaa gcgtctaaac ggacgttggg gttttgctag
    19261 tttctttaga gaaaactagc taagtcttta atgttatcat tagagatggc ataaatataa
    19321 tacttgtgtc tgctgataag atcattttaa tttggacgat tagacttgtt gaactacagg
    19381 ttactgaatc acttgcgcta atcaacatgg gagatatgta cgatgaatca tttgacaagt
    19441 cgggcggtcc tgctgacttg atggacgatt cttgggtgga atcagtttcg tggaaagatc
    19501 tgttgaagaa gttacacagc ataaaatttg cactacagtc tggtagagat gagatcactg
    19561 ggttactagc ggcactgaat agacagtgtc cttattcacc atatgagcag tttccagata
    19621 agaaggtgta tttcctttta gactcacggg ctaacagtgc tcttggtgtg attcagaacg
    19681 cttcagcgtt caagagacga gctgatgaga agaatgcagt ggcgggtgtt acaaatattc
    19741 ctgcgaatcc aaacacaacg gttacgacga accaagggag tactactact accaaggcga
    19801 acactggctc gactttggaa gaagacttgt acacttatta caaattcgat gatgcctcta
    19861 cagctttcca caaatctcta acttcgttag agaacatgga gttgaagagt tattaccgaa
    19921 ggaactttga gaaagtattc gggattaagt ttggtggagc agctgctagt tcatctgcac
    19981 cgcctccagc gagtggaggt ccgatacgtc ctaatcccaa gctttaggga tttaaggacg
    20041 tgaactctgt tgagatctct gtgaaattca gagggtgggt gataccatat tcactgatgc
    20101 cattagcgac atctaaatag ggctaattgt gactaatttg agggaatttc ctttaccatt
    20161 gacgtcagtg tcgttggtag catttgagtt tcgcaatgca cgaattactt aggaagtggc
    20221 ttgacgacac taatgtgtta ttgttagata atggtttggt ggtcaaggta cgtagtagag
    20281 tcccacatat tcgcacgtat gaagtaattg gaaagttgtc agtttttgat aattcactgg
    20341 gagatgat
//
