LOCUS       sequence-476827-       20210 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This all-in-one vector is used to specifically silence the NbPDS 
            gene via virus-induced gene silencing (VIGS)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 20210)
  AUTHORS   Hao Y, Yuan Z, Li Y, Zuo D, Cheng H, Wang Q, Zhang Y, Lv L, Liu J, 
            Song G
  TITLE     An all-in-one plant virus-based vector toolkit for streamlined gene 
            silencing, overexpression, and genome editing.
  JOURNAL   Plant Commun. 2025 May 19:101379. doi: 10.1016/j.xplc.2025.101379.
  PUBMED    40394900
REFERENCE   2  (bases 1 to 20210)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Commun. 2025 May 19:101379. doi: 10.1016/j.xplc.2025.101379."
COMMENT     Sequence Label: pVS2-NbPDS
FEATURES             Location/Qualifiers
     source          1..20210
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1075..1092
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      1127..1379
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(1388..1404)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1388..1404)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     misc_feature    1425..1449
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             1914..2564
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     primer_bind     complement(2752..2769)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(3003..3022)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     oriT            3245..3354
                     /note="incP origin of transfer"
     CDS             3387..3758
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     rep_origin      3885..4596
                     /label=oriV
                     /note="incP origin of replication"
     mobile_element  5219..5986
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     CDS             6178..6972
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     CDS             7271..8419
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKTYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    10015..10039
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     primer_bind     complement(10286..10307)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     10498..10517
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10642..10664
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     10656..10673
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     10657..10673
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(10682..10934)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        complement(17788..18133)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     promoter        18663..18709
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
ORIGIN
        1 acgctgtttg agggaaaagt agagaacgta tttgttttta tgttcaggcg gttcttgtgt
       61 gtcaacaaag atggacattg ttactcaagg aagcacgatg agctttatta ttacggacga
      121 gtggacttag attctgtgag taaggttacc gaattggcca gttaacgcta gcgaatggca
      181 cttaacttca taaaccctga cgagctttcg atgcagtgca ttttgattgc tttgaacaga
      241 tttcttcagg agaaacatgg ttcaaaaatg gcctttttag atggtaaccc tcctgagaga
      301 ctttgcatgc cgattgtgga acatattgag tcaaaaggtg gccaagtcag actaaactca
      361 cgaataaaaa agatcgagct gaatgaggat ggaagtgtca aatgttttat actgaataat
      421 ggcagtacaa ttaaaggaga tgcttttgtg tttgccactc cagtggatat cttgaagctt
      481 cttttgcctg aagactggaa agagatccca tatttccaaa agttggagaa gctagtggga
      541 gttcctgtga taaatgtcca tatatggttt gacagaaaac tgaagactag tctgcaggca
      601 tgccattcga ggcccgggca tgtcccgaag acattaaact acggttcttt aagtagatcc
      661 gtgtctgaag ttttaggttc aatttaaacc tacgagattg acattctcga ctgatcttga
      721 ttgatcggta agtcttttgt aatttaattt tctttttgat tttattttaa attgttatct
      781 gtttctgtgt atagactgtt tgagatcggc gtttggccga ctcattgtct taccataggg
      841 gaacggactt tgtttgtgtt gttattttat ttgtatttta ttaaaattct caacgatctg
      901 aaaaagcctc gcggctaaga gattgttggg gggtgagtaa gtacttttaa agtgatgatg
      961 gttacaaagg caaaaggggt aaaacccctc gcctacgtaa gcgttattac gcccgtctgt
     1021 acttatatca gtacactgac gagtccctaa aggacgaaac gggagaacgc tagccaccac
     1081 caccaccacc acgtgtgaat tacaggtgac cagctcgaat ttccccgatc gttcaaacat
     1141 ttggcaataa agtttcttaa gattgaatcc tgttgccggt cttgcgatga ttatcatata
     1201 atttctgttg aattacgtta agcatgtaat aattaacatg taatgcatga cgttatttat
     1261 gagatgggtt tttatgatta gagtcccgca attatacatt taatacgcga tagaaaacaa
     1321 aatatagcgc gcaaactagg ataaattatc gcgcgcggtg tcatctatgt tactagatcg
     1381 ggaattggtc atagctgttt cctgggatcc aaactatcag tgtttgacag gatatattgg
     1441 cgggtaaacc taagagaaaa gagcgtttat tagaataatc ggatatttaa aagggcgtga
     1501 aaaggtttat ccgttcgtcc atttgtatgt gcatgccaac cacagggttc cccagatctg
     1561 gcgccggcca gcgagacgag caagattggc cgccgcccga aacgatccga cagcgcgccc
     1621 agcacaggtg cgcaggcaaa ttgcaccaac gcatacagcg ccagcagaat gccatagtgg
     1681 gcggtgacgt cgttcgagtg aaccagatcg cgcaggaggc ccggcagcac cggcataatc
     1741 aggccgatgc cgacagcgtc gagcgcgaca gtgctcagaa ttacgatcag gggtatgttg
     1801 ggtttcacgt ctggcctccg gaccagcctc cgctggtccg attgaacgcg cggattcttt
     1861 atcactgata agttggtgga catattatgt ttatcagtga taaagtgtca agcatgacaa
     1921 agttgcagcc gaatacagtg atccgtgccg ccctggacct gttgaacgag gtcggcgtag
     1981 acggtctgac gacacgcaaa ctggcggaac ggttgggggt tcagcagccg gcgctttact
     2041 ggcacttcag gaacaagcgg gcgctgctcg acgcactggc cgaagccatg ctggcggaga
     2101 atcatacgca ttcggtgccg agagccgacg acgactggcg ctcatttctg atcgggaatg
     2161 cccgcagctt caggcaggcg ctgctcgcct accgcgatgg cgcgcgcatc catgccggca
     2221 cgcgaccggg cgcaccgcag atggaaacgg ccgacgcgca gcttcgcttc ctctgcgagg
     2281 cgggtttttc ggccggggac gccgtcaatg cgctgatgac aatcagctac ttcactgttg
     2341 gggccgtgct tgaggagcag gccggcgaca gcgatgccgg cgagcgcggc ggcaccgttg
     2401 aacaggctcc gctctcgccg ctgttgcggg ccgcgataga cgccttcgac gaagccggtc
     2461 cggacgcagc gttcgagcag ggactcgcgg tgattgtcga tggattggcg aaaaggaggc
     2521 tcgttgtcag gaacgttgaa ggaccgagaa agggtgacga ttgatcagga ccgctgccgg
     2581 agcgcaaccc actcactaca gcagagccat gtagacaaca tcccctcccc ctttccaccg
     2641 cgtcagacgc ccgtagcagc ccgctacggg ctttttcatg ccctgcccta gcgtccaagc
     2701 ctcacggccg cgctcggcct ctctggcggc cttctggcgc tcttccgctt cctcgctcac
     2761 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     2821 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     2881 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     2941 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     3001 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     3061 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc ttttccgctg
     3121 cataaccctg cttcggggtc attatagcga ttttttcggt atatccatcc tttttcgcac
     3181 gatatacagg attttgccaa agggttcgtg tagactttcc ttggtgtatc caacggcgtc
     3241 agccgggcag gataggtgaa gtaggcccac ccgcgagcgg gtgttccttc ttcactgtcc
     3301 cttattcgca cctggcggtg ctcaacggga atcctgctct gcgaggctgg ccggctaccg
     3361 ccggcgtaac agatgagggc aagcggatgg ctgatgaaac caagccaacc aggaagggca
     3421 gcccacctat caaggtgtac tgccttccag acgaacgaag agcgattgag gaaaaggcgg
     3481 cggcggccgg catgagcctg tcggcctacc tgctggccgt cggccagggc tacaaaatca
     3541 cgggcgtcgt ggactatgag cacgtccgcg agctggcccg catcaatggc gacctgggcc
     3601 gcctgggcgg cctgctgaaa ctctggctca ccgacgaccc gcgcacggcg cggttcggtg
     3661 atgccacgat cctcgccctg ctggcgaaga tcgaagagaa gcaggacgag cttggcaagg
     3721 tcatgatggg cgtggtccgc ccgagggcag agccatgact tttttagccg ctaaaacggc
     3781 cggggggtgc gcgtgattgc caagcacgtc cccatgcgct ccatcaagaa gagcgacttc
     3841 gcggagctgg tgaagtacat caccgacgag caaggcaaga ccgagcgcct ttccgacgct
     3901 caccgggctg gttgccctcg ccgctgggct ggcggccgtc tatggccctg caaacgcgcc
     3961 agaaacgccg tcgaagccgt gtgcgagaca ccgcggccgc cggcgttgtg gatacctcgc
     4021 ggaaaacttg gccctcactg acagatgagg ggcggacgtt gacacttgag gggccgactc
     4081 acccggcgcg gcgttgacag atgaggggca ggctcgattt cggccggcga cgtggagctg
     4141 gccagcctcg caaatcggcg aaaacgcctg attttacgcg agtttcccac agatgatgtg
     4201 gacaagcctg gggataagtg ccctgcggta ttgacacttg aggggcgcga ctactgacag
     4261 atgaggggcg cgatccttga cacttgaggg gcagagtgct gacagatgag gggcgcacct
     4321 attgacattt gaggggctgt ccacaggcag aaaatccagc atttgcaagg gtttccgccc
     4381 gtttttcggc caccgctaac ctgtctttta acctgctttt aaaccaatat ttataaacct
     4441 tgtttttaac cagggctgcg ccctgtgcgc gtgaccgcgc acgccgaagg ggggtgcccc
     4501 cccttctcga accctcccgg cccgctaacg cgggcctccc atccccccag gggctgcgcc
     4561 cctcggccgc gaacggcctc accccaaaaa tggcagcgct ggcagtcctt gccattgccg
     4621 ggatcggggc agtaacggga tgggcgatca gcccgagcgc gacgcccgga agcattgacg
     4681 tgccgcaggt gctggcatcg acattcagcg accaggtgcc gggcagtgag ggcggcggcc
     4741 tgggtggcgg cctgcccttc acttcggccg tcggggcatt cacggacttc atggcggggc
     4801 cggcaatttt taccttgggc attcttggca tagtggtcgc gggtgccgtg ctcgtgttcg
     4861 ggggtgcgat aaacccagcg aaccatttga ggtgataggt aagattatac cgaggtatga
     4921 aaacgagaat tggaccttta cagaattact ctatgaagcg ccatatttaa aaagctacca
     4981 agacgaagag gatgaagagg atgaggaggc agattgcctt gaatatattg acaatactga
     5041 taagataata tatcttttat atagaagata tcgccgtatg taaggatttc agggggcaag
     5101 gcataggcag cgcgcttatc aatatatcta tagaatgggc aaagcataaa aacttgcatg
     5161 gactaatgct tgaaacccag gacaataacc ttatagcttg taaattctat cataattggg
     5221 taatgactcc aacttattga tagtgtttta tgttcagata atgcccgatg actttgtcat
     5281 gcagctccac cgattttgag aacgacagcg acttccgtcc cagccgtgcc aggtgctgcc
     5341 tcagattcag gttatgccgc tcaattcgct gcgtatatcg cttgctgatt acgtgcagct
     5401 ttcccttcag gcgggattca tacagcggcc agccatccgt catccatatc accacgtcaa
     5461 agggtgacag caggctcata agacgcccca gcgtcgccat agtgcgttca ccgaatacgt
     5521 gcgcaacaac cgtcttccgg agactgtcat acgcgtaaaa cagccagcgc tggcgcgatt
     5581 tagccccgac atagccccac tgttcgtcca tttccgcgca gacgatgacg tcactgcccg
     5641 gctgtatgcg cgaggttacc gactgcggcc tgagtttttt aagtgacgta aaatcgtgtt
     5701 gaggccaacg cccataatgc gggctgttgc ccggcatcca acgccattca tggccatatc
     5761 aatgattttc tggtgcgtac cgggttgaga agcggtgtaa gtgaactgca gttgccatgt
     5821 tttacggcag tgagagcaga gatagcgctg atgtccggcg gtgcttttgc cgttacgcac
     5881 caccccgtca gtagctgaac aggagggaca gctgatagaa acagaagcca ctggagcacc
     5941 tcaaaaacac catcatacac taaatcagta agttggcagc atcacccata attgtggttt
     6001 caaaatcggc tccgtcgata ctatgttata cgccaacttt gaaaacaact ttgaaaaagc
     6061 tgttttctgg tatttaaggt tttagaatgc aaggaacagt gaattggagt tcgtcttgtt
     6121 ataattagct tcttggggta tctttaaata ctgtagaaaa gaggaaggaa ataataaatg
     6181 gctaaaatga gaatatcacc ggaattgaaa aaactgatcg aaaaataccg ctgcgtaaaa
     6241 gatacggaag gaatgtctcc tgctaaggta tataagctgg tgggagaaaa tgaaaaccta
     6301 tatttaaaaa tgacggacag ccggtataaa gggaccacct atgatgtgga acgggaaaag
     6361 gacatgatgc tatggctgga aggaaagctg cctgttccaa aggtcctgca ctttgaacgg
     6421 catgatggct ggagcaatct gctcatgagt gaggccgatg gcgtcctttg ctcggaagag
     6481 tatgaagatg aacaaagccc tgaaaagatt atcgagctgt atgcggagtg catcaggctc
     6541 tttcactcca tcgacatatc ggattgtccc tatacgaata gcttagacag ccgcttagcc
     6601 gaattggatt acttactgaa taacgatctg gccgatgtgg attgcgaaaa ctgggaagaa
     6661 gacactccat ttaaagatcc gcgcgagctg tatgattttt taaagacgga aaagcccgaa
     6721 gaggaacttg tcttttccca cggcgacctg ggagacagca acatctttgt gaaagatggc
     6781 aaagtaagtg gctttattga tcttgggaga agcggcaggg cggacaagtg gtatgacatt
     6841 gccttctgcg tccggtcgat cagggaggat atcggggaag aacagtatgt cgagctattt
     6901 tttgacttac tggggatcaa gcctgattgg gagaaaataa aatattatat tttactggat
     6961 gaattgtttt agtacctaga tgtggcgcaa cgatgccggc gacaagcagg agcgcaccga
     7021 cttcttccgc atcaagtgtt ttggctctca ggccgaggcc cacggcaagt atttgggcaa
     7081 ggggtcgctg gtattcgtgc agggcaagat tcggaatacc aagtacgaga aggacggcca
     7141 gacggtctac gggaccgact tcattgccga taaggtggat tatctggaca ccaaggcacc
     7201 aggcgggtca aatcaggaat aagggcacat tgccccggcg tgagtcgggg caatcccgca
     7261 aggagggtga atgaatcgga cgtttgaccg gaagacatac aggcaagaac tgatcgacgc
     7321 ggggttttcc gccgaggatg ccgaaaccat cgcaagccgc accgtcatgc gtgcgccccg
     7381 cgaaaccttc cagtccgtcg gctcgatggt ccagcaagct acggccaaga tcgagcgcga
     7441 cagcgtgcaa ctggctcccc ctgccctgcc cgcgccatcg gccgccgtgg agcgttcgcg
     7501 tcgtctcgaa caggaggcgg caggtttggc gaagtcgatg accatcgaca cgcgaggaac
     7561 tatgacgacc aagaagcgaa aaaccgccgg cgaggacctg gcaaaacagg tcagcgaggc
     7621 caagcaggcc gcgttgctga aacacacgaa gcagcagatc aaggaaatgc agctttcctt
     7681 gttcgatatt gcgccgtggc cggacacgat gcgagcgatg ccaaacgaca cggcccgctc
     7741 tgccctgttc accacgcgca acaagaaaat cccgcgcgag gcgctgcaaa acaaggtcat
     7801 tttccacgtc aacaaggacg tgaagatcac ctacaccggc gtcgagctgc gggccgacga
     7861 tgacgaactg gtgtggcagc aggtgttgga gtacgcgaag cgcaccccta tcggcgagcc
     7921 gatcaccttc acgttctacg agctttgcca ggacctgggc tggtcgatca atggccggta
     7981 ttacacgaag gccgaggaat gcctgtcgcg cctacaggcg acggcgatgg gcttcacgtc
     8041 cgaccgcgtt gggcacctgg aatcggtgtc gctgctgcac cgcttccgcg tcctggaccg
     8101 tggcaagaaa acgtcccgtt gccaggtcct gatcgacgag gaaatcgtcg tgctgtttgc
     8161 tggcgaccac tacacgaaat tcatatggga gaagtaccgc aagctgtcgc cgacggcccg
     8221 acggatgttc gactatttca gctcgcaccg ggagccgtac ccgctcaagc tggaaacctt
     8281 ccgcctcatg tgcggatcgg attccacccg cgtgaagaag tggcgcgagc aggtcggcga
     8341 agcctgcgaa gagttgcgag gcagcggcct ggtggaacac gcctgggtca atgatgacct
     8401 ggtgcattgc aaacgctagg gccttgtggg gtcagttccg gctgggggtt cagcagccag
     8461 cgctttactg gcatttcagg aacaagcggg cactgctcga cgcacttgct tcgctcagta
     8521 tcgctcggga cgcacggcgc gctctacgaa ctgccgataa acagaggatt aaaattgaca
     8581 attgtgatta aggctcagat tcgacggctt ggagcggccg acgtgcagga tttccgcgag
     8641 atccgattgt cggccctgaa gaaagctcca gagatgttcg ggtccgttta cgagcacgag
     8701 gagaaaaagc ccatggaggc gttcgctgaa cggttgcgag atgccgtggc attcggcgcc
     8761 tacatcgacg gcgagatcat tgggctgtcg gtcttcaaac aggaggacgg ccccaaggac
     8821 gctcacaagg cgcatctgtc cggcgttttc gtggagcccg aacagcgagg ccgaggggtc
     8881 gccggtatgc tgctgcgggc gttgccggcg ggtttattgc tcgtgatgat cgtccgacag
     8941 attccaacgg gaatctggtg gatgcgcatc ttcatcctcg gcgcacttaa tatttcgcta
     9001 ttctggagct tgttgtttat ttcggtctac cgcctgccgg gcggggtcgc ggcgacggta
     9061 ggcgctgtgc agccgctgat ggtcgtgttc atctctgccg ctctgctagg tagcccgata
     9121 cgattgatgg cggtcctggg ggctatttgc ggaactgcgg gcgtggcgct gttggtgttg
     9181 acaccaaacg cagcgctaga tcctgtcggc gtcgcagcgg gcctggcggg ggcggtttcc
     9241 atggcgttcg gaaccgtgct gacccgcaag tggcaacctc ccgtgcctct gctcaccttt
     9301 accgcctggc aactggcggc cggaggactt ctgctcgttc cagtagcttt agtgtttgat
     9361 ccgccaatcc cgatgcctac aggaaccaat gttctcggcc tggcgtggct cggcctgatc
     9421 ggagcgggtt taacctactt cctttggttc cgggggatct cgcgactcga acctacagtt
     9481 gtttccttac tgggctttct cagccccaga tctggggtcg atcagccggg gatgcatcag
     9541 gccgacagtc ggaacttcgg gtccccgacc tgtaccattc ggtgagcaat ggatagggga
     9601 gttgatatcg tcaacgttca cttctaaaga aatagcgcca ctcagcttcc tcagcggctt
     9661 tatccagcga tttcctatta tgtcggcata gttctcaaga tcgacagcct gtcacggtta
     9721 agcgagaaat gaataagaag gctgataatt cggatctctg cgagggagat gatatttgat
     9781 cacaggcagc aacgctctgt catcgttaca atcaacatgc taccctccgc gagatcatcc
     9841 gtgtttcaaa cccggcagct tagttgccgt tcttccgaat agcatcggta acatgagcaa
     9901 agtctgccgc cttacaacgg ctctcccgct gacgccgtcc cggactgatg ggctgcctgt
     9961 atcgagtggt gattttgtgc cgagctgccg gtcggggagc tgttggctgg ctggtggcag
    10021 gatatattgt ggtgtaaaca aattgacgct tagacaactt aataacacat tgcggacgtt
    10081 tttaatgtac tggggtggtt tttcttttca ccagtgagac gggcaacagc tgattgccct
    10141 tcaccgcctg gccctgagag agttgcagca agcggtccac gctggtttgc cccagcaggc
    10201 gaaaatcctg tttgatggtg gttccgaaat cggcaaaatc ccttataaat caaaagaata
    10261 gcccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt
    10321 ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc
    10381 atcacccaaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa
    10441 agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg
    10501 gaagaaagcg aaaggagcgg gcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg
    10561 tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa
    10621 gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgaattcc
    10681 cgatctagta acatagatga caccgcgcgc gataatttat cctagtttgc gcgctatatt
    10741 ttgttttcta tcgcgtatta aatgtataat tgcgggactc taatcataaa aacccatctc
    10801 ataaataacg tcatgcatta catgttaatt attacatgct taacgtaatt caacagaaat
    10861 tatatgataa tcatcgcaag accggcaaca ggattcaatc ttaagaaact ttattgccaa
    10921 atgtttgaac gatcggggaa attcgagggg cccgtttcgt cctttaggga ctcgtcagtg
    10981 tactgatata agtacagacg ggcgtaataa cgcttacgta ggcgaggggt tttacccctt
    11041 ttgcctttgt aaccatcatc actttaaaag tacttactca ccccccaata atctcttagc
    11101 ctcgaggcct tttcagatca ttgagaattt taataaaata caaataaaat aacaacacaa
    11161 acaaagtccg ttcccctatg gtaagacaat gagtcggcca agcgccaatc tcaaacagtc
    11221 tatatacaga aacagataac aatatataat aaaatcaaaa agcaaacaaa cgatcaatcc
    11281 cgaaaggaac atcatctctt aaaaatcttt ttggtgtccc aaattctctg tcctctttga
    11341 atttcttctt agaggtcttc gaattatgag actttttagc tgtcgcagac atgttttcag
    11401 cttgatttcg tcgaatttgt ttccaaattt ctcgatttct tgtttcaaca cgtttacgac
    11461 acttttcaag gtgactacgg ccacaacaat tatacacatc aaaagtaaaa tcattgataa
    11521 caacacagac aaaccatcca caattatttt ccgcacacct acgtgtgaca ccaaccatgt
    11581 cagcaacttg ctttcgcaat ttgttagcat gaccgatact acacgtctcg tgaccaagaa
    11641 cagtgacttc attcacacaa cccttgagta cacacgtcat attgacaata aatttcttta
    11701 tgcattaaga cgagtttttc ttattagacg ctctcttaac atcttcactt gacaataatg
    11761 acttaggtct agtagtagct ggtctgtaag gaccatcgta cttcgcctca atcgtcttca
    11821 tctccacttg tttcaaagac atgaactcac cgaaaacctc ttgatcggta aaatcgcctt
    11881 caatgtaatc cgaactgttc agttcttcca cattgaggcg cctagtcgaa tcagtagcaa
    11941 cccatattag cgacacttcg atactgaaag ggtatgtacc gtcacagacc ggaacgccct
    12001 cagaaataca acccaccttc cagacatcaa gtacgttctc ctgtgtggaa atactgtatt
    12061 caaccttgta ctgcgcaact gaacagttat cgcaagctcg agctttgaat ctctgaatag
    12121 tctttcctct ctttcttttc tcagtaagtc tcgaatccaa cagatcgatt tgaatgaacc
    12181 caggcgtatc tgcaggtaga tgtgatgtta caaccaattg tacacctact atctttagaa
    12241 acgcgtaaga tttaatcatt ttgtaatccc tttggtcaaa aacgtctaac ttgtgttcac
    12301 tcttcttccc accaacatga tttaggacag tttctcttct tctgtgaaaa tacttcggcc
    12361 tcttcctcct accgtccaca aacaattcaa tggcccctag atttgagaat tttgagactt
    12421 cgaaagtctt cttcttcaag gtgaccaatg acttgtcttc catattacca gtcgtaaaag
    12481 tttgacacag ctctctgtgc cttcttccaa tcaaccttag ccgggttcaa ttccttatcg
    12541 ttttcgtctc ggaataatgt acacagagct gtaaaactct taatatgctt ccatagcgca
    12601 caaagcgcat gaacagaatc acctttgtac aaataccggt ctgaaacaga ctcggacagt
    12661 ttggatacca ccatgtagtt cccaagagct ctattggaat cattcagcga gatgtagatc
    12721 tcggctaaat gttgcacatc ctttatactc tttttcccca acttcgtcag aacttttacc
    12781 ggatctggca cgaactcgta acacgatgac gtcttaagca agaacttccc acaaaacatt
    12841 gggacatcgt acttaaaaat cttgcactcg aaattccact tagtagccaa ctttggacac
    12901 ggatcaacaa actgcgttcc tctaggaaac gcaatcagtg agtcatctcc tccatatgta
    12961 accatgactg ctttctccaa tggtaattca gacaagagtg cacacagtgt tctatctgaa
    13021 tttgcattat aagtatcagc atctccactc ttttgttggt accaaatatg cgccatcata
    13081 ccattttgaa tatctctcac agtagtttga gtgtgcgaca cctcccaaag gaaggccgcc
    13141 cactcatcta gcccaaataa cctgtaaatc tccagctgca gttgtaaatg gaagcgattt
    13201 gccgacttgt cgaatttaga catgtctatc tcaacaaagt cgtaagccgc ttccgtattt
    13261 aagaacttca ctcgatcgtt taaatcactc gatgtcattc tcgtgttgaa cacaaaatgt
    13321 ggttgcatag catccaactt gcgttcatta atttctttga aaattggacc gaacaacgag
    13381 ttgatcaact tctcgtgata cacaacagtc tgtagagctg agtattcaaa ttgcggtgtt
    13441 aaatcagtct taggtttcac gtcagatttg atcatataca tgtaagacga atagtctatt
    13501 tcatgtaact ctctcacatc tgctactacc ttagcctgta ccgctgtgct ttgatttctc
    13561 caccatctct ccatttgagc tctattgaca ataggatcag cccgaatttt tcccacatcg
    13621 tagacaacag atttcagctt cttcatcgtc tcttcgatta gaactgtcgc gtgcacattt
    13681 tcttgtagat cgggtgccgc ttggttcctt ttctgcaatg ctaacaaact ctccaactga
    13741 gtagtcttcc ttttgtcagg catagcagta cgaataaccg gtttcagaaa cgtttctttt
    13801 tcagcaaact tgtctttcca gtttccactt ttgatcgtaa cattgtctaa tcgcaaattg
    13861 caatcagtcg ttgccaccaa atacccgtct aggcttgagt ctcttaacga atttcccgga
    13921 aacaaagcgt cgtaccacat ctccaagtcc gtaatcgtac ctgaatctgg aacggcgcaa
    13981 ggcccttcat gatgtctaaa gacatcaaac ctagaccgaa accgtcataa gaccgtctca
    14041 gtaacaaaaa atcttgccaa agccgccttc gtaagcgcgg cactttccct gatctctttg
    14101 cttacatcgt cctctttcac agtttcatac acaagtgatt gtgtgtgacg cgacaacgcc
    14161 acgattaagt actcccgacc tctagcgatt gagtcatccg taggtttcgt cctgactagg
    14221 actacatctt tgaatgtctc tccttgcgac tcgtgtactg tactcacttt agctgccact
    14281 ttcctcaatg ccaacaaatt taccaactcc ttcttctcag actgaaggaa agttatgtac
    14341 tgagcaccag gcttcaaaga aacctgttct ttagacacaa tcttcctcac cgtcatcgaa
    14401 ttagcagtcg cgttatgtgt tctgacatct ccagtatagt acttgttcaa tacggcagcc
    14461 acatctgctg gacttctgta agtttctctt ttttctgtaa caatgtcaaa ctttccgacc
    14521 agactagaaa acctcaaatc aacttgagat accctaggct tgaaagaaat ttgattctga
    14581 tctccttgac agatacatcg tttagcacca gctatctgag cgcaaaagta caccatacca
    14641 gcatgggcca tgagagcttc atcgaaatgc aacacgtctc cggttaaaga accatcaaca
    14701 caatgcatca aaaaagaatc aaccgtcttc actctccttt tcaatttgca tggaaaacct
    14761 ttgctcgcga atctctcgat caagtcgtcg gttgctgctc tcccagtaga gagaaccaca
    14821 tcgacacaag gattagctga gttgacaatc attgtcgact taccacaacc aggtacaccg
    14881 tcaactaatt caaaagaaca gttaactggt cctagatcca gggcacttaa attgtccaag
    14941 atcaacctgt tattgcaaag atacgtctga tcgcaaacta tgacataatc actcatcctt
    15001 tcgaccgcga agtcgttgac caacatacct cgttcccaaa cagccacttc taagtatccc
    15061 gaccaatcat ccatacgttt gtctggttgg aacacataag catgttcagt gaccactgga
    15121 cgtatgcacc agttcttcat ctccacatcc cacactccca gtccgtgcgg gtaattctga
    15181 cctccagttc gaataactcc tctcgaatag tcacgataca gagacataat ttcatcattt
    15241 tgagcgactc tctccgcatc taagtagtac agaaactctc ttctagcgtc cacagaatcg
    15301 tctccactga caaccggcct ttttggtaat ctctcacctc ccatcacttg gaagtaatcg
    15361 acacgtttga ccgtttttcc tttgctgacc aaaggtttga cagctggcaa cacgttctca
    15421 aatcctggtg gcgttaaagg tttacacaac acctttgtac cgcttgtttc ccctctttca
    15481 cattcagctg aaaaggctga aacaacctcg tcaatttctg cttcagtgtc aataagacca
    15541 ctcagaaact gctccatgat ctcctcatcg tctacctttc ttgaaactcc aactctagct
    15601 tgggtcagca cgttactgtc ttccaacaac agacccagtc cgcttcgaga tgaaccgacc
    15661 accgatggtg gagaagttct caccgaactg ccagaatagt tagagaattt tgcacttaga
    15721 aactcgtcag cgatttcttt cttctgctgc aatagcttat cagacttggc ttttacctcg
    15781 gataaatctg gtaaaaccgt ctcaccggac tcagatgccg aatacagact gctaaagaaa
    15841 tcgttgattt cataatagcg gcacggattc tcaacgtcta agacatgggc cccccgtttg
    15901 atatcaaaca tatcacatag ttttttcacc agtccatctc ggatgtctcg acgcataagc
    15961 gtgattcctc caatggcata cacaacactc agaaatacta tcaccttggt tttgtgcgag
    16021 ttctcgtaac atagttctgg accatttggt gtgtccttcg cccaggttag taccttcttt
    16081 ggatagaatc caattagagt accaaccatg ccaacgaatc cgccgactgc gatcttgtta
    16141 gcaaactgct tccacagttt tcttgatatt aaacccagca catcagtcaa tgacgtgata
    16201 ggtttcgtct cagtcagaat tgcttcacgc aactccctca tgacaggtcg cgccacttgt
    16261 tccttcacta gcaaagtttg agccaacaac tgtaaatctc gagaatctac actttgcttg
    16321 ttcttcacgg ccgccccgtt tatgaataag acccaattat ttgaactcaa gtatgattta
    16381 acattgctta tggtcagctg ctggtcagat aacctagcta tgtaatccaa acacttgtcc
    16441 atgaattgtt tctctacaaa caggtctttc ttgaccaact ctcgcgttga ttcgaccaga
    16501 tcgaaaattg ggacaacaac catgttttcc catctactga tatatattct tcggtagtac
    16561 tcttgcgatg atagcgacct cctcggaact ccagctatcc tgtagatcga aaaaagcatt
    16621 gtgtctcctc tcacctgcca cggttcgaag tagaacacat tcccttggta actcactggc
    16681 ttccccagta gatacttctt gtactcttcc cagtcatgaa tgtaagaaaa ggaaggatct
    16741 ttctcaaaac cgaaatagat cttcccgttc ttcttcatgt agtaaccgtc aacagaaggt
    16801 aatggacctt cgtccactaa catactttca ggagcaaaaa gcatagcagc atgaaccact
    16861 ttagttttct tctcaaccat agtagccatc aagtctttca acttgaagtc atacaggctg
    16921 tgaagagcta tcgcatactg cgcgcctttc ttcttaccat cagaaaaacc cctacatgta
    16981 caaccctgat atgtattatt gcacctaacc gcgttaggcg cagcaatatt ttcacgaaat
    17041 tggtcataca tccacagctc attctccgac atctgtctct taccacttct agcttgatca
    17101 ccaatagcag taagaaatat ctgtcgagaa atttctctct gatcatcccg aatatctaga
    17161 tttggacaac aggaatgaat ccttttctca cctctagtta cccaaaacga ccattgacca
    17221 ccgatgtcat ccacctcttg cagagcagga actctatcta aaagcaataa cgcttgtagt
    17281 tttcgctcag cagccgcaaa cccatgaacc atgtttttgt cgtctttaaa cacgatattg
    17341 aattcaggat aaatttccat caacttgttc tgttccttct gagaaagaac ttcatgaaca
    17401 atcactggat ctttagtctt tctttctcta atcatgtcat cgactttatc aacaacaact
    17461 ctttgcatca tttgcccgat ctcacaatca ggtttagtca gcatcaagtc aaagaaatga
    17521 ctcctctgct cagcagacat ctcgtccaca ttgagcaatt gagacaactt gaagttaccg
    17581 ttcgccattg tatctgtatg tatttttaca aagtatcaaa gaaaaggttt taggcacacc
    17641 agtcactttc ccccactcgg tactaagctg gatagattaa accaaaaaga aaaacaaaat
    17701 aaaactgtag gtgataagta cgttctaccg cgttctcacc aaaatccaat tagcacgttc
    17761 taccgcgttc aaaggattga aatgttttat cctctccaaa tgaaatgaac ttccttatat
    17821 agaggaaggg tcttgcgaag gatagtggga ttgtgcgtca tcccttacgt cagtggagat
    17881 atcacatcaa tccacttgct ttgaagacgt ggttggaacg tcttcttttt ccacgatgtt
    17941 cctcgtgggt gggggtccat ctttgggacc actgtcggta gaggcatctt gaacgatagc
    18001 ctttccttta tcgcaatgat ggcatttgta gaagccatct tccttttcta ctgtcctttc
    18061 gatgaagtga cagatagctg ggcaatggaa tccgaggagg tttcccgata ttaccctttg
    18121 ttgaaaagtc tcaatagccc tctggtcttc tgagactgta tctttgatat tcttggagta
    18181 gacgagagtg tcgtgctcca ccatgttgac catcacatca atccacttgc tttgaagacg
    18241 tggttggaac gtcttctttt tccacgatgt tcctcgtggg tgggggtcca tctttgggac
    18301 cactgtcggt agaggcatct tgaacgatag cctttccttt atcgcaatga tggcatttgt
    18361 agaagccatc ttccttttct actgtccttt cgatgaagtg acagatagct gggcaatgga
    18421 atccgaggag gtttcccgat attacccttt gttgaaaagt ctcaatagcc ctctggtctt
    18481 ctgagactgt atctttgata ttcttggagt agacgagagt gtcgtgctcc accatgttga
    18541 cctgcaggca tgcgcaaatt taaagcgctg atatcgatcg cgcgcagatc tgtcatgatg
    18601 atcattgcaa ttggatccat ctccactgac gtaagggatg acgcacaatc ccactatcct
    18661 tcgcaagacc cttcctctat ataaggaagt tcatttcatt tggagaggat aaaacattgc
    18721 acctatggtg ttgccctggc tggggtatgt cagtgatcgc agtagaatgt actaattgac
    18781 aagttggaga atacggtaga acgtccttat ccaacacagc ctttatccct ctccctgacg
    18841 aggtttttgt cagtgtaata tttctttttg aactatccag cttagtaccg tacgggaaag
    18901 tgactggtgt gcttatcttt gaaatgttac tttgggtttc ggttctttag gttagtaaga
    18961 aagcacttgt cttctcatac aaaggaaaac ctgagacgta tcgcttacga aagtagcaat
    19021 gaaagaaagg tggtggtttt aatcgctacc gcaaaaacga tggggtcgtt ttaattaact
    19081 tctcctacgc aagcgtctaa acggacgttg gggttttgct agtttcttta gagaaaacta
    19141 gctaagtctt taatgttatc attagagatg gcataaatat aatacttgtg tctgctgata
    19201 agatcatttt aatttggacg attagacttg ttgaactaca ggttactgaa tcacttgcgc
    19261 taatcaacat gggagatatg tacgatgaat catttgacaa gtcgggcggt cctgctgact
    19321 tgatggacga ttcttgggtg gaatcagttt cgtggaaaga tctgttgaag aagttacaca
    19381 gcataaaatt tgcactacag tctggtagag atgagatcac tgggttacta gcggcactga
    19441 atagacagtg tccttattca ccatatgagc agtttccaga taagaaggtg tatttccttt
    19501 tagactcacg ggctaacagt gctcttggtg tgattcagaa cgcttcagcg ttcaagagac
    19561 gagctgatga gaagaatgca gtggcgggtg ttacaaatat tcctgcgaat ccaaacacaa
    19621 cggttacgac gaaccaaggg agtactacta ctaccaaggc gaacactggc tcgactttgg
    19681 aagaagactt gtacacttat tacaaattcg atgatgcctc tacagctttc cacaaatctc
    19741 taacttcgtt agagaacatg gagttgaaga gttattaccg aaggaacttt gagaaagtat
    19801 tcgggattaa gtttggtgga gcagctgcta gttcatctgc accgcctcca gcgagtggag
    19861 gtccgatacg tcctaatccc aagctttagg gatttaagga cgtgaactct gttgagatct
    19921 ctgtgaaatt cagagggtgg gtgataccat attcactgat gccattagcg acatctaaat
    19981 agggctaatt gtgactaatt tgagggaatt tcctttacca ttgacgtcag tgtcgttggt
    20041 agcatttgag tttcgcaatg cacgaattac ttaggaagtg gcttgacgac actaatgtgt
    20101 tattgttaga taatggtttg gtggtcaagg tacgtagtag agtcccacat attcgcacgt
    20161 atgaagtaat tggaaagttg tcagtttttg ataattcact gggagatgat
//
