LOCUS       sequence-47683-e         815 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Young CL, Raden DL, Caplan JL, Czymmek KJ, Robinson AS
  TITLE     Cassette series designed for live-cell imaging of proteins and 
            high-resolution techniques in yeast.
  JOURNAL   Yeast. 2012 Mar;29(3-4):119-36. doi: 10.1002/yea.2895. Epub 2012 Apr
            4.
  PUBMED    22473760
REFERENCE   2  (bases 1 to 815)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast."; 
            date: "2012-03"; volume: "29(3-4):119-36. doi"; pages: " 
            10.1002/yea.2895. Epub 2012 Apr 4"
COMMENT     Sequence Label: pCY 3040-05
FEATURES             Location/Qualifiers
     source          1..815
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(527..546)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(528..546)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(633..650)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tgatatttgt acataaactt tataaatgaa attcataata gaaacgacac gaaattacaa
       61 aatggaatat gttcataggg tagacgaaac tatatacgca atctacatac atttatcaag
      121 aaggagaaaa aggaggatgt aaaggaatac aggtaagcaa attgatacta atggctcaac
      181 gtgataagga aaaagaattg cactttaaca ttaatattga caaggaggag ggcaccacac
      241 aaaaagttag gtgtaacaga aaatcatgaa actatgattc ctaatttata tattggagga
      301 ttttctctaa aaaaaaaaaa atacaacaaa taaaaaacac tcaatgacct gaccatttga
      361 tggagtttaa gtcaatacct tcttgaacca tttcccataa tggtgaaagt tccctcaaga
      421 attttactct gtcagaaacg gccttaacga cgtagtcgat cgagctcgaa ttcatcgatg
      481 atatcagatc cactagtggc ctatgcggcc gcggatctgc cggtctccct atagtgagtc
      541 gtattaattt cgataagcca ggttaacctg cattaatgaa tcggccaacg cgcggggaga
      601 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
      661 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
      721 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
      781 aaaaaggccg cgttgctggc gtttttccat aggct
//
