LOCUS       sequence-476830-        8799 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cytosine base editor with codon-optimized S. aureus KKH Cas9, 
            rAPOBEC1 deaminase, and 2x UGI. EGFP can be used as a transfection 
            marker..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8799)
  TITLE     Elucidating the genetic mechanisms governing cytosine base editing 
            outcomes through CRISPRi screens
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8799)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pSG1040-rA1-SaKKH-2xUGI_P2A_EGFP
FEATURES             Location/Qualifiers
     source          1..8799
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(171..962,963..1031))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     794..813
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1032..1136)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1215..1234)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        1406..1785
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1786..1989
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1940..1960
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2031..2050
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2031..2049
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2099..2119
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2120..2803
                     /codon_start=1
                     /product="cytidine deaminase (C to U editing enzyme) from 
                     rat"
                     /label=APOBEC-1
                     /note="can use ssDNA as a substrate (Komor et al., 2016)"
                     /translation="SSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINWGG
                     RHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSRAITEFLS
                     RYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSPSNE
                     AHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPHIL
                     WATGLK"
     CDS             6086..6334
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             6365..6613
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             6656..6676
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6686..6742
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(6743..6745,6746..6748,6749..7462)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6743..6745,6746..6748,6749..7459)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6788..6809)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7049..7068)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7396..7417
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             7468..7485
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(7508..7525)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7514..7738
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7809..7825)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7809..7825)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7822..7844)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7833..7849
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7857..7863,7864..7881,7882..7887))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7902..7923
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8040..8057)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8211..8799)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8291..8310)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gaagatcctt tgatcttttc tacggggtct gacactcagt ggaacgaaaa ctcacgttaa
       61 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
      121 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
      181 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
      241 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
      301 atgataccgc gggacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
      361 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
      421 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
      481 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
      541 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
      601 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
      661 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
      721 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
      781 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
      841 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
      901 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
      961 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     1021 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     1081 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     1141 tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atcgatctcc cgatccccta
     1201 gggtcgactc tcagtacaat ctgctctgat gccgcatagt taagccagta tctgctccct
     1261 gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa
     1321 ggcttgaccg acaattgcat gaagaatctg cttagggtta ggcgttttgc gctgcttcgc
     1381 gatgtacggg ccagatatac gcgttgacat tgattattga ctagttatta atagtaatca
     1441 attacggggt cattagttca tagcccatat attgagttcc gcgttacata acttacggta
     1501 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
     1561 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
     1621 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
     1681 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
     1741 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg
     1801 cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc
     1861 attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt
     1921 aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
     1981 agcagagctg gtttagtgaa ccgtcagatc cgctagagat ccgcggccgc taatacgact
     2041 cactataggg agagccgcca ccatgaaacg gacagccgac ggaagcgagt tcgagtcacc
     2101 aaagaagaag cggaaagtca gctcagagac tggcccagtg gctgtggacc ccacattgag
     2161 acggcggatc gagccccatg agtttgaggt attcttcgat ccgagagagc tccgcaagga
     2221 gacctgcctg ctttacgaaa ttaattgggg gggccggcac tccatttggc gacatacatc
     2281 acagaacact aacaagcacg tcgaagtcaa cttcatcgag aagttcacga cagaaagata
     2341 tttctgtccg aacacaaggt gcagcattac ctggtttctc agctggagcc catgcggcga
     2401 atgtagtagg gccatcactg aattcctgtc aaggtatccc cacgtcactc tgtttattta
     2461 catcgcaagg ctgtaccacc acgctgaccc ccgcaatcga caaggcctgc gggatttgat
     2521 ctcttcaggt gtgactatcc aaattatgac tgagcaggag tcaggatact gctggagaaa
     2581 ctttgtgaat tatagcccga gtaatgaagc ccactggcct aggtatcccc atctgtgggt
     2641 acgactgtac gttcttgaac tgtactgcat catactgggc ctgcctcctt gtctcaacat
     2701 tctgagaagg aagcagccac agctgacatt ctttaccatc gctcttcagt cttgtcatta
     2761 ccagcgactg cccccacaca ttctctgggc caccgggttg aaatccggag gatctagcgg
     2821 aggctcctct ggctctgaga cacctggcac aagcgagagc gcaacacctg aaagcagcgg
     2881 gggcagcagc ggggggtcag ggaagcgaaa ttacattctg gggctggcca ttggcattac
     2941 atcagtgggc tatggcatca ttgactacga gacaagggac gtgatcgacg ccggcgtgag
     3001 actgttcaag gaggccaacg tggagaacaa tgagggccgg agatccaaga ggggagcaag
     3061 gcgcctgaag cggagaaggc gccacagaat ccagagagtg aagaagctgc tgttcgatta
     3121 caacctgctg accgaccact ccgagctgtc tggcatcaat ccttatgagg ccagagtgaa
     3181 gggcctgtcc cagaagctgt ctgaggagga gtttagcgcc gccctgctgc acctggcaaa
     3241 gaggagaggc gtgcacaacg tgaatgaggt ggaggaggac accggcaacg agctgtccac
     3301 aaaggagcag atcagccgca attccaaggc cctggaggag aagtatgtgg ccgagctgca
     3361 gctggagcgg ctgaagaagg atggcgaggt gaggggctcc atcaatcgct tcaagacctc
     3421 tgactacgtg aaggaggcca agcagctgct gaaggtgcag aaggcctacc accagctgga
     3481 tcagtccttt atcgatacat atatcgacct gctggagaca aggcgcacat actatgaggg
     3541 accaggagag ggctctccct tcggctggaa ggacatcaag gagtggtacg agatgctgat
     3601 gggccactgc acctattttc cagaggagct gagaagcgtg aagtacgcct ataacgccga
     3661 tctgtacaac gccctgaatg acctgaacaa cctggtcatc accagggatg agaacgagaa
     3721 gctggagtac tatgagaagt tccagatcat cgagaacgtg ttcaagcaga agaagaagcc
     3781 tacactgaag cagatcgcca aggagatcct ggtgaacgag gaggacatca agggctaccg
     3841 cgtgacctcc acaggcaagc cagagttcac caatctgaag gtgtatcacg atatcaagga
     3901 catcacagcc cggaaggaga tcatcgagaa cgccgagctg ctggatcaga tcgccaagat
     3961 cctgaccatc tatcagagct ccgaggacat ccaggaggag ctgaccaacc tgaatagcga
     4021 gctgacacag gaggagatcg agcagatcag caatctgaag ggctacaccg gcacacacaa
     4081 cctgagcctg aaggccatca atctgatcct ggatgagctg tggcacacaa acgacaatca
     4141 gatcgccatc tttaaccggc tgaagctggt gccaaagaag gtggacctgt cccagcagaa
     4201 ggagatccca accacactgg tggacgattt catcctgtct cccgtggtga agcggagctt
     4261 catccagagc atcaaagtga tcaacgccat catcaagaag tacggcctgc ccaatgatat
     4321 catcatcgag ctggccaggg agaagaactc caaggacgcc cagaagatga tcaatgagat
     4381 gcagaagagg aaccgccaga ccaatgagcg gatcgaggag atcatcagaa ccacaggcaa
     4441 ggagaacgcc aagtacctga tcgagaagat caagctgcac gatatgcagg agggcaagtg
     4501 tctgtattct ctggaggcca tccctctgga ggacctgctg aacaatccat tcaactacga
     4561 ggtggatcac atcatccccc ggagcgtgag cttcgacaat tcttttaaca ataaggtgct
     4621 ggtgaagcag gaggagaaca gcaagaaggg caataggacc cctttccagt acctgtctag
     4681 ctccgattct aagatcagct acgagacatt caagaagcac atcctgaatc tggccaaggg
     4741 caagggccgc atcagcaaga ccaagaagga gtacctgctg gaggagcggg acatcaacag
     4801 attctccgtg cagaaggact tcatcaaccg gaatctggtg gacaccagat acgccacacg
     4861 cggcctgatg aatctgctgc ggtcttattt cagagtgaac aatctggatg tgaaggtgaa
     4921 gagcatcaac ggcggcttca cctcctttct gcggagaaag tggaagttta agaaggagcg
     4981 caacaagggc tataagcacc acgccgagga tgccctgatc atcgccaatg ccgacttcat
     5041 ctttaaggag tggaagaagc tggacaaggc caagaaagtg atggagaacc agatgttcga
     5101 ggagaagcag gccgagagca tgcccgagat cgagacagag caggagtaca aggagatttt
     5161 catcacacct caccagatca agcacatcaa ggacttcaag gactacaagt attctcacag
     5221 ggtggataag aagcccaacc gcaagctgat caatgacacc ctgtatagca cacggaagga
     5281 cgataagggc aataccctga tcgtgaacaa tctgaacggc ctgtacgaca aggataatga
     5341 caagctgaag aagctgatca acaagtctcc cgagaagctg ctgatgtacc accacgatcc
     5401 tcagacatat cagaagctga agctgatcat ggagcagtac ggcgacgaga agaacccact
     5461 gtataagtac tatgaggaga caggcaacta cctgacaaag tatagcaaga aggataatgg
     5521 ccccgtgatc aagaagatca agtactatgg caacaagctg aatgcccacc tggacatcac
     5581 cgacgattac cctaactctc gcaataaggt ggtgaagctg agcctgaagc cataccggtt
     5641 cgacgtgtac ctggacaacg gcgtgtataa gtttgtgaca gtgaagaatc tggatgtgat
     5701 caagaaggag aactactatg aggtgaacag caagtgctac gaggaggcca agaagctgaa
     5761 gaagatcagc aaccaggccg agttcatcgc ctctttttac aagaatgacc tgatcaagat
     5821 caatggcgag ctgtatagag tgatcggcgt gaacaatgat ctgctgaaca gaatcgaagt
     5881 gaatatgatc gacatcacct acagggagta tctggagaac atgaatgata agaggccccc
     5941 tcatatcatc aagaccatcg cctctaagac acagagcatc aagaagtaca gcacagacat
     6001 cctggggaac ctgtatgaag tcaagagcaa gaaacatcct cagattatca agaaaagtgg
     6061 cgggagcggc gggagcggag ggagcactaa tctgagcgac atcattgaga aggagactgg
     6121 gaaacagctg gtcattcagg agtccatcct gatgctgcct gaggaggtgg aggaagtgat
     6181 cggcaacaag ccagagtctg acatcctggt gcacaccgcc tacgacgagt ccacagatga
     6241 gaatgtgatg ctgctgacct ctgacgcccc cgagtataag ccttgggccc tggtcatcca
     6301 ggattctaac ggcgagaata agatcaagat gctgagcgga ggatccggag gatctggagg
     6361 cagcaccaac ctgtctgaca tcatcgagaa ggagacaggc aagcagctgg tcatccagga
     6421 gagcatcctg atgctgcccg aagaagtcga agaagtgatc ggaaacaagc ctgagagcga
     6481 tatcctggtc cataccgcct acgacgagag taccgacgaa aatgtgatgc tgctgacatc
     6541 cgacgcccca gagtataagc cctgggctct ggtcatccag gattccaacg gagagaacaa
     6601 aatcaaaatg ctgtctggcg gctcaaaaag aaccgccgac ggcagcgaat tcgagcccaa
     6661 gaagaagagg aaagtcggaa gcggagctac taacttcagc ctgctgaagc aggctggaga
     6721 cgtggaggag aaccctggac ctatggtgag caagggcgag gagctgttca ccggggtggt
     6781 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga
     6841 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa
     6901 gctgcccgtg ccctggccca ccctcgtgac caccctgacc tatggagtgc agtgcttcag
     6961 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
     7021 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
     7081 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
     7141 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat
     7201 catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga
     7261 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc
     7321 cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa
     7381 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg
     7441 catggacgag ctgtacaagt aaccggtcat catcaccatc accattgagt ttaaacccgc
     7501 tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg
     7561 ccttccttga ccctgaaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt
     7621 gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc
     7681 aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct
     7741 tctgaggcgg aaagaaccag ctggggctcg ataccgtcga cctctagcta gagcttggcg
     7801 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     7861 atacaagccg gaagcataaa gtgtaaagcc tagggtgcct aatgagtgag ctaactcaca
     7921 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     7981 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgcac ttccgcttcc
     8041 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     8101 aaggcggtaa tacggttatc cacagaatca gaggataacg caggaaagaa catgtgagca
     8161 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     8221 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     8281 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     8341 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     8401 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     8461 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     8521 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     8581 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     8641 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     8701 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     8761 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaa
//
