LOCUS       sequence-476846-       13313 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This all-in-one vector is used to express the EGFP gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13313)
  AUTHORS   Hao Y, Yuan Z, Li Y, Zuo D, Cheng H, Wang Q, Zhang Y, Lv L, Liu J, 
            Song G
  TITLE     An all-in-one plant virus-based vector toolkit for streamlined gene 
            silencing, overexpression, and genome editing.
  JOURNAL   Plant Commun. 2025 May 19:101379. doi: 10.1016/j.xplc.2025.101379.
  PUBMED    40394900
REFERENCE   2  (bases 1 to 13313)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Commun. 2025 May 19:101379. doi: 10.1016/j.xplc.2025.101379."
COMMENT     Sequence Label: pVT-EGFP
FEATURES             Location/Qualifiers
     source          1..13313
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    938..1111
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(join(1159..1872,1873..1875,1876..1878))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(1162..1872,1873..1875,1876..1878))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1204..1225)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     1553..1572
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1812..1833
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     promoter        complement(1953..2629)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(1996..2016)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     primer_bind     complement(6399..6416)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6399..6415)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6408..6430)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    6618..6642
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             7943..8572
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             9001..10074
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      10140..10334
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(10570..10592)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    10678..10818
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     10733..10752
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(10833..10850)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11004..11592)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11084..11103)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(11679..12473)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    12898..12922
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     protein_bind    13190..13211
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(13226..13231,13232..13249,13250..13256)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13264..13280
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     13269..13291
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     13288..13304
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     13288..13304
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aattcgagct cattcgagct ccagaacgat ctcagttagg tcatgggaaa gctgatattc
       61 atccctctga gactctatat aattaaatgc gtttggggga ttagctaatt gagaatccat
      121 atagagaaat atggccgcgc agcgacgccg gttgaagaat atgaacaagg aataagatga
      181 acaaggaaga agatgaacaa gcaataattt aattaattat tgttaataag cataaattat
      241 gaaaataatt ttgatggtaa tgaaagtatt ataaataata tttaggagaa aatattgtta
      301 aggaaatatc tggttatgtt tatttgttaa ggtatatggg tttttataga aacccaggag
      361 cagctatggt aagaaaccca ggactcaaaa aatgcctctc aacttctctc atatgttgcg
      421 gagtctggag tcccatttat accatagaaa gcattggggg tatagcatgg taatttcaca
      481 tcggactcca taccgcggcc atctatataa tattactgat ggccgcgcga agtcccggta
      541 tgccttttca gccgttggat ctggagccgt acctttttcg cgttcccatt ttgcccctgg
      601 agtcccgagc tatttacgac cccctgccac cgagtcccct tcgtggagcg ctcttcgtcc
      661 aacacgtgtc ccaacttttg cgcatttttg actacctgtc gcgcacttga attaaagttg
      721 cccttttatt ttcttccaat tattttgcgg ctgagtagtc tagataattg tgacaactta
      781 gtgagcaagt tgtaggagga ccctatccgt ctgggaaaat aattgggttt tcacgtgtac
      841 caatgggaaa taatatgtat agcctattta atttagaaaa ttaaatagtt ttagtaattc
      901 ctatataata gcgtttacta gtctgggtaa aattcgtgat ctggatttta gtatggattt
      961 tggttttagg aattagaaat tttattgata gaagtatttt acaaatacaa atacatacta
     1021 agggtttctt atatgctcaa cacatgagcg aaaccctata ggaaccctaa ttcccttatc
     1081 tgggaactac tcacacatta ttatggagaa actcgagctt gtcgatcgac agatcccggt
     1141 cggcatctac tcgagacctt acttgtacag ctcgtccatg ccgagagtga tcccggcggc
     1201 ggtcacgaac tccagcagga ccatgtgatc gcgcttctcg ttggggtctt tgctcagggc
     1261 ggactgggtg ctcaggtagt ggttgtcggg cagcagcacg gggccgtcgc cgatgggggt
     1321 gttctgctgg tagtggtcgg cgagctgcac gctgccgtcc tcgatgttgt ggcggatctt
     1381 gaagttcacc ttgatgccgt tcttctgctt gtcggccatg atatagacgt tgtggctgtt
     1441 gtagttgtac tccagcttgt gccccaggat gttgccgtcc tccttgaagt cgatgccctt
     1501 cagctcgatg cggttcacca gggtgtcgcc ctcgaacttc acctcggcgc gggtcttgta
     1561 gttgccgtcg tccttgaaga agatggtgcg ctcctggacg tagccttcgg gcatggcgga
     1621 cttgaagaag tcgtgctgct tcatgtggtc ggggtagcgg ctgaagcact gcacgccgta
     1681 ggtcagggtg gtcacgaggg tgggccaggg cacgggcagc ttgccggtgg tgcagatgaa
     1741 cttcagggtc agcttgccgt aggtggcatc gccctcgccc tcgccggaca cgctgaactt
     1801 gtggccgttt acgtcgccgt ccagctcgac caggatgggc accaccccgg tgaacagctc
     1861 ctcgcccttg ctcaccatgg tctcggatct cattgccccc cggatctgcg aaagctcgag
     1921 agagatagat ttgtagagag agactggtga tttcagcgtg tcctctccaa atgaaatgaa
     1981 cttccttata tagaggaagg gtcttgcgaa ggatagtggg attgtgcgtc atcccttacg
     2041 tcagtggaga tatcacatca atccacttgc tttgaagacg tggttggaac gtcttctttt
     2101 tccacgatgc tcctcgtggg tgggggtcca tctttgggac cactgtcggc agaggcatct
     2161 tgaacgatag cctttccttt atcgcaatga tggcatttgt aggtgccacc ttccttttct
     2221 actgtccttt tgatgaagtg acagatagct gggcaatgga atccgaggag gtttcccgat
     2281 attacccttt gttgaaaagt ctcaatagcc ctttggtctt ctgagactgt atctttgata
     2341 ttcttggagt agacgagagt gtcgtgctcc accatgttca catcaatcca cttgctttga
     2401 agacgtggtt ggaacgtctt ctttttccac gatgctcctc gtgggtgggg gtccatcttt
     2461 gggaccactg tcggcagagg catcttgaac gatagccttt cctttatcgc aatgatggca
     2521 tttgtaggtg ccaccttcct tttctactgt ccttttgatg aagtgacaga tagctgggca
     2581 atggaatccg aggaggtttc ccgatattac cctttgttga aaagtctcaa tagccctttg
     2641 gtcttctgag actgtatctt tgatattctt ggagtagacg agagtgtcgt gctccaccat
     2701 gttggcaagc tgctctagct cacgttgacg gaaactgcag aacgatctca gttaggtcat
     2761 gggaaagctg atattcatcc ctctgagact ctatataatt aaatgcgttt gggggattag
     2821 ctaattgaga atccatatag agaaatatgg ccgcgcagcg acgccggttg aagaatatga
     2881 acaaggaata agatgaacaa ggaagaagat gaacaagcaa taatttaatt aattattgtt
     2941 aataagcata aattatgaaa ataattttga tggtaatgaa agtattataa ataatattta
     3001 ggagaaaata ttgttaagga aatatctggt tatgtttatt tgttaaggta tatgggtttt
     3061 tatagaaacc caggagcagc tatggtaaga aacccaggac tcaaaaaatg cctctcaact
     3121 tctctcatat gttgcggagt ctggagtccc atttatacca tagaaagcat tgggggtata
     3181 gcatggtaat ttcacatcgg actccatacc gcggccatct atataatatt actgatggcc
     3241 gcgcgaagtc ccggtatgcc ttttcagccg ttggatctgg agccgtacct ttttcgcgtt
     3301 cccattttgc ccctggagtc ccgagctatt tacgaccccc tgccaccgag tccccttcgt
     3361 ggagcgctct tcgtccaaca cgtgtcccaa cttttgcgca tttttgacta cctgtcgcgc
     3421 acttgaatta aagttgccct tttattttct tccaattatt ttgcggctga gtagtctagt
     3481 ggatcccccg gagctccact tgggataggt taagaaaata tgtctggctt gtaaacgaaa
     3541 agaatcgggg tttctaggca ttttttaaat agtgacccag gactcaaaaa ctagctctca
     3601 acttctctca tatgttgtgg agtctggagt cctttatata tgtttacgct atagaaggca
     3661 tttggggact ctcccgggcc caccatagga ctccataccg cggccatcta tataatatta
     3721 ctgatggccg cgcggatttt ggagccccct tttgtcgtta gcgcgacctt taattcaaat
     3781 taaaggttgc ttttttctat cgtccgatca ttttgcgcct gactagccta gatattagca
     3841 acaacttcgc ggctaagttg ttgaccgtca agtataaatt aatgggctta tggcctaatg
     3901 ggtatagagc aaaatggcat gcctgcagac tagtgcttta ctctgatccg cagatatttc
     3961 atcaggaaag agctttgcaa gttccttcat tgtagcatca ataatttctg agtcactaca
     4021 tgcaatccat tcttctgctg gggcaaaaac taactccaac atggattggt ttggattgta
     4081 atattcctta catgttacag acatgtcagc ataaacactt aaaagcgggc ttctgctaaa
     4141 gagtagatga tcataggtgt tcttcaattt cctatcaaac cagatgtgaa cgttgataac
     4201 tggaactcca actaatttct ctaatttctt gaagtatgaa atctctctcc agtcttcagg
     4261 cactagtgaa ttcgctagcg ttaactggcc ataatcctgt gtatgcaacg ttgaaaattc
     4321 ggatctattt ttacgattcg atcatgaatt aataaaattt aaattttatt gaatgatttt
     4381 ctagtacata actgacatac gacttatcag ttgcgaaacg aacagctcta attacattgt
     4441 taagtgaaat tacgcctaat tcgtctaagt acaacataac caactgttta aacctaatta
     4501 aataagtcga cccagaagct gtcagagatg tcgtccagac ttggaagttc aggtaagctt
     4561 tgtggagatg caacgctctc cgtaagttgt ggttgaatcg gatctggacg tggtatatac
     4621 gcgtcctggt gtatagcaga tcctctactc cgtatatctt gaaatatagg ggatttatta
     4681 tctcccagat atacacgcca ttctctgcct gacgtgcagt gatgatttcc cctgtgcgtg
     4741 aatccatgac cggtgcagcc tatgtggaag tatatggtgc acccacagtt tagatcaatg
     4801 cgtcgtcgtc taatcgccct cctcttggct tgcctgtgtg ccttcttgat agaggggggc
     4861 gtcgagagtg atgaagataa cattcttgag agtccaatgt ttgagcgacg cattttccgc
     4921 tttatccagg aaatctttat agctggatcc ctcgcctgga ttgcaaagca cgattgaagg
     4981 gatccctcct ttaatttgaa ctggtttccc gtacttgcaa tttgactgcc agtctttttg
     5041 ggccccaaga agctctttcc aatgctttag ctttaggtat tgcggtgcta cgtcatcaat
     5101 gacgttatag gcaacattat tcgagtacac tcgattattg aagtccaggt gtccactgag
     5161 gtaattatgt gggcctaaag cacgagccca catcgtcttg cccgttctag aatcaccttc
     5221 cactatgaga ctaactggtc ttacaggccg cgcagcggga cccattccaa aataatcatc
     5281 cgcccattct tgcatctgtt caggaacgtt agtgaaagag gaaagtggaa atggagggac
     5341 ccattgttca ggcgcctttt tgaaaaggcg ttccaagttc gctgtaatgt tatggtaact
     5401 cacgatgaac gcccttggat ctccggcccg gataattgca agagcttccg ccaaactagg
     5461 tgcattgatg gcgttataat agacgtcgtc tttattcttc tttgtacccc caggcacttt
     5521 gaactgtccg gattcacaac aatcgccgtc tttggtgatg taattcttga cggcgttggc
     5581 gtctttggct gcttgtatgt ttgggtgaaa attggtcgac cttctggggt gagtaaggtc
     5641 gaaaaatctc ttattcttga tgttggactt tccagagagt tggatgagac agtgaaggtg
     5701 tgggaatccg tccgagtgct cctctctggc aactcgaata tatgttggtt tgacgactga
     5761 ccatgataag gtttgaagca tttgaagagc ttcatttttt gggatatcgc acttgggata
     5821 ggttaagaaa atatgtctgg cttgtaaacg aaaagaatcg gggtttctag gcatttttta
     5881 aatagtgacc caggactcaa aaactagctc tcaacttctc tcatatgttg tggagtctgg
     5941 agtcctttat atatgtttac gctatagaag gcatttgggg actctcccgg gcccaccata
     6001 ggactccata ccgcggccat ctatataata ttactgatgg ccgcgcggat tttggagccc
     6061 ccttttgtcg ttagcgcgac ctttaattca aattaaaggt tgcttttttc tatcgtccaa
     6121 tcattttgcg cctgactagc ctagatatta gcaacaactt cgcgactaag ttgttgaccg
     6181 tcaagtataa attaatgggc ttatggccca atgggtatag agcaaaatgc ccaagcgtga
     6241 tgccccgtgg cgcttattag cgggtacgtc taaggtaagt cgaaatgcca actactctcc
     6301 cttgagagta ttgggcccta aagttaataa gggacctcga gggggggccc ggtacccggg
     6361 gatcctctag agtcgacctg caggcatgca agcttggcac tggccgtcgt tttacaacgt
     6421 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
     6481 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
     6541 ctgaatggcg aatgctagag cagcttgagc ttggatcaga ttgtcgtttc ccgccttcag
     6601 tttaaactat cagtgtttga caggatatat tggcgggtaa acctaagaga aaagagcgtt
     6661 tattagaata atcggatatt taaaagggcg tgaaaaggtt tatccgttcg tccatttgta
     6721 tgtgcatgcc aaccacaggg ttcccctcgg gatcaaagta ctttgatcca acccctccgc
     6781 tgctatagtg cagtcggctt ctgacgttca gtgcagccgt cttctgaaaa cgacatgtcg
     6841 cacaagtcct aagttacgcg acaggctgcc gccctgccct tttcctggcg ttttcttgtc
     6901 gcgtgtttta gtcgcataaa gtagaatact tgcgactaga accggagaca ttacgccatg
     6961 aacaagagcg ccgccgctgg cctgctgggc tatgcccgcg tcagcaccga cgaccaggac
     7021 ttgaccaacc aacgggccga actgcacgcg gccggctgca ccaagctgtt ttccgagaag
     7081 atcaccggca ccaggcgcga ccgcccggag ctggccagga tgcttgacca cctacgccct
     7141 ggcgacgttg tgacagtgac caggctagac cgcctggccc gcagcacccg cgacctactg
     7201 gacattgccg agcgcatcca ggaggccggc gcgggcctgc gtagcctggc agagccgtgg
     7261 gccgacacca ccacgccggc cggccgcatg gtgttgaccg tgttcgccgg cattgccgag
     7321 ttcgagcgtt ccctaatcat cgaccgcacc cggagcgggc gcgaggccgc caaggcccga
     7381 ggcgtgaagt ttggcccccg ccctaccctc accccggcac agatcgcgca cgcccgcgag
     7441 ctgatcgacc aggaaggccg caccgtgaaa gaggcggctg cactgcttgg cgtgcatcgc
     7501 tcgaccctgt accgcgcact tgagcgcagc gaggaagtga cgcccaccga ggccaggcgg
     7561 cgcggtgcct tccgtgagga cgcattgacc gaggccgacg ccctggcggc cgccgagaat
     7621 gaacgccaag aggaacaagc atgaaaccgc accaggacgg ccaggacgaa ccgtttttca
     7681 ttaccgaaga gatcgaggcg gagatgatcg cggccgggta cgtgttcgag ccgcccgcgc
     7741 acgtctcaac cgtgcggctg catgaaatcc tggccggttt gtctgatgcc aagctggcgg
     7801 cctggccggc cagcttggcc gctgaagaaa ccgagcgccg ccgtctaaaa aggtgatgtg
     7861 tatttgagta aaacagcttg cgtcatgcgg tcgctgcgta tatgatgcga tgagtaaata
     7921 aacaaatacg caaggggaac gcatgaaggt tatcgctgta cttaaccaga aaggcgggtc
     7981 aggcaagacg accatcgcaa cccatctagc ccgcgccctg caactcgccg gggccgatgt
     8041 tctgttagtc gattccgatc cccagggcag tgcccgcgat tgggcggccg tgcgggaaga
     8101 tcaaccgcta accgttgtcg gcatcgaccg cccgacgatt gaccgcgacg tgaaggccat
     8161 cggccggcgc gacttcgtag tgatcgacgg agcgccccag gcggcggact tggctgtgtc
     8221 cgcgatcaag gcagccgact tcgtgctgat tccggtgcag ccaagccctt acgacatatg
     8281 ggccaccgcc gacctggtgg agctggttaa gcagcgcatt gaggtcacgg atggaaggct
     8341 acaagcggcc tttgtcgtgt cgcgggcgat caaaggcacg cgcatcggcg gtgaggttgc
     8401 cgaggcgctg gccgggtacg agctgcccat tcttgagtcc cgtatcacgc agcgcgtgag
     8461 ctacccaggc actgccgccg ccggcacaac cgttcttgaa tcagaacccg agggcgacgc
     8521 tgcccgcgag gtccaggcgc tggccgctga aattaaatca aaactcattt gagttaatga
     8581 ggtaaagaga aaatgagcaa aagcacaaac acgctaagtg ccggccgtcc gagcgcacgc
     8641 agcagcaagg ctgcaacgtt ggccagcctg gcagacacgc cagccatgaa gcgggtcaac
     8701 tttcagttgc cggcggagga tcacaccaag ctgaagatgt acgcggtacg ccaaggcaag
     8761 accattaccg agctgctatc tgaatacatc gcgcagctac cagagtaaat gagcaaatga
     8821 ataaatgagt agatgaattt tagcggctaa aggaggcggc atggaaaatc aagaacaacc
     8881 aggcaccgac gccgtggaat gccccatgtg tggaggaacg ggcggttggc caggcgtaag
     8941 cggctgggtt gcctgccggc cctgcaatgg cactggaacc cccaagcccg aggaatcggc
     9001 gtgagcggtc gcaaaccatc cggcccggta caaatcggcg cggcgctggg tgatgacctg
     9061 gtggagaagt tgaaggccgc gcaggccgcc cagcggcaac gcatcgaggc agaagcacgc
     9121 cccggtgaat cgtggcaagc ggccgctgat cgaatccgca aagaatcccg gcaaccgccg
     9181 gcagccggtg cgccgtcgat taggaagccg cccaagggcg acgagcaacc agattttttc
     9241 gttccgatgc tctatgacgt gggcacccgc gatagtcgca gcatcatgga cgtggccgtt
     9301 ttccgtctgt cgaagcgtga ccgacgagct ggcgaggtga tccgctacga gcttccagac
     9361 gggcacgtag aggtttccgc agggccggcc ggcatggcca gtgtgtggga ttacgacctg
     9421 gtactgatgg cggtttccca tctaaccgaa tccatgaacc gataccggga agggaaggga
     9481 gacaagcccg gccgcgtgtt ccgtccacac gttgcggacg tactcaagtt ctgccggcga
     9541 gccgatggcg gaaagcagaa agacgacctg gtagaaacct gcattcggtt aaacaccacg
     9601 cacgttgcca tgcagcgtac gaagaaggcc aagaacggcc gcctggtgac ggtatccgag
     9661 ggtgaagcct tgattagccg ctacaagatc gtaaagagcg aaaccgggcg gccggagtac
     9721 atcgagatcg agctagctga ttggatgtac cgcgagatca cagaaggcaa gaacccggac
     9781 gtgctgacgg ttcaccccga ttactttttg atcgatcccg gcatcggccg ttttctctac
     9841 cgcctggcac gccgcgccgc aggcaaggca gaagccagat ggttgttcaa gacgatctac
     9901 gaacgcagtg gcagcgccgg agagttcaag aagttctgtt tcaccgtgcg caagctgatc
     9961 gggtcaaatg acctgccgga gtacgatttg aaggaggagg cggggcaggc tggcccgatc
    10021 ctagtcatgc gctaccgcaa cctgatcgag ggcgaagcat ccgccggttc ctaatgtacg
    10081 gagcagatgc tagggcaaat tgccctagca ggggaaaaag gtcgaaaagg tgtctttcct
    10141 gtggatagca cgtacattgg gaacccaaag ccgtacattg ggaaccggaa cccgtacatt
    10201 gggaacccaa agccgtacat tgggaaccgg tcacacatgt aagtgactga tataaaagag
    10261 aaaaaaggcg atttttccgc ctaaaactct ttaaaactta ttaaaactct taaaacccgc
    10321 ctggcctgtg cataactgtc tggccagcgc acagccgaag agctgcaaaa agcgcctacc
    10381 cttcggtcgc tgcgctccct acgccccgcc gcttcgcgtc ggcctatcgc ggccgctggc
    10441 cgctcaaaaa tggctggcct acggccaggc aatctaccag ggcgcggaca agccgcgccg
    10501 tcgccactcg accgccggcg cccacatcaa ggcaccctgc ctcgcgcgtt tcggtgatga
    10561 cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga
    10621 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc
    10681 agccatgacc cagtcacgta gcgatagcgg agtgtatact ggcttaacta tgcggcatca
    10741 gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg
    10801 agaaaatacc gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
    10861 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
    10921 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
    10981 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
    11041 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
    11101 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
    11161 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
    11221 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
    11281 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
    11341 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
    11401 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
    11461 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
    11521 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
    11581 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
    11641 aactcacgtt aagggatttt ggtcatgcat tctaggtact aaaacaattc atccagtaaa
    11701 atataatatt ttattttctc ccaatcaggc ttgatcccca gtaagtcaaa aaatagctcg
    11761 acatactgtt cttccccgat atcctccctg atcgaccgga cgcagaaggc aatgtcatac
    11821 cacttgtccg ccctgccgct tctcccaaga tcaataaagc cacttacttt gccatctttc
    11881 acaaagatgt tgctgtctcc caggtcgccg tgggaaaaga caagttcctc ttcgggcttt
    11941 tccgtcttta aaaaatcata cagctcgcgc ggatctttaa atggagtgtc ttcttcccag
    12001 ttttcgcaat ccacatcggc cagatcgtta ttcagtaagt aatccaattc ggctaagcgg
    12061 ctgtctaagc tattcgtata gggacaatcc gatatgtcga tggagtgaaa gagcctgatg
    12121 cactccgcat acagctcgat aatcttttca gggctttgtt catcttcata ctcttccgag
    12181 caaaggacgc catcggcctc actcatgagc agattgctcc agccatcatg ccgttcaaag
    12241 tgcaggacct ttggaacagg cagctttcct tccagccata gcatcatgtc cttttcccgt
    12301 tccacatcat aggtggtccc tttataccgg ctgtccgtca tttttaaata taggttttca
    12361 ttttctccca ccagcttata taccttagca ggagacattc cttccgtatc ttttacgcag
    12421 cggtattttt cgatcagttt tttcaattcc ggtgatattc tcattttagc catttattat
    12481 ttccttcctc ttttctacag tatttaaaga taccccaaga agctaattat aacaagacga
    12541 actccaattc actgttcctt gcattctaaa accttaaata ccagaaaaca gctttttcaa
    12601 agttgttttc aaagttggcg tataacatag tatcgacgga gccgattttg aaaccgcggt
    12661 gatcacaggc agcaacgctc tgtcatcgtt acaatcaaca tgctaccctc cgcgagatca
    12721 tccgtgtttc aaacccggca gcttagttgc cgttcttccg aatagcatcg gtaacatgag
    12781 caaagtctgc cgccttacaa cggctctccc gctgacgccg tcccggactg atgggctgcc
    12841 tgtatcgagt ggtgattttg tgccgagctg ccggtcgggg agctgttggc tggctggtgg
    12901 caggatatat tgtggtgtaa acaaattgac gcttagacaa cttaataaca cattgcggac
    12961 gtttttaatg tactgaatta acgccgaatt aattcgggga tagccctttg gtcttctgag
    13021 actgtatctt tgatattctt ggagtagacg agagtgtcgt gctccaccat gttggcaagc
    13081 tgctctagcc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
    13141 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
    13201 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
    13261 gaattgtgag cggataacaa tttcacacag gaaacagcta tgacatgatt acg
//
