LOCUS       sequence-476851-       14520 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Deactivated ribozyme split GFPuv expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14520)
  AUTHORS   Liu Y, Rajput R, Islam MT, Valle ID, Yao T, Agrawal R, Boone BA, 
            Eckert CA, Abraham PE, Chen JG, Tuskan GA, Yang X
  TITLE     A split ribozyme system for in vivo plant RNA imaging and genetic 
            engineering.
  JOURNAL   Plant Biotechnol J. 2025 May;23(5):1640-1649. doi: 
            10.1111/pbi.14612. Epub 2025 Feb 7.
  PUBMED    39919021
REFERENCE   2  (bases 1 to 14520)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/pbi.14612";
            journalName: "Plant Biotechnol J"; date: "2025-05"; volume: "23"; 
            issue: "5"; pages: "1640-1649"
COMMENT     Sequence Label: pXYB031
FEATURES             Location/Qualifiers
     source          1..14520
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(16..361)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     723..745
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     737..754
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     738..754
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1269..1614
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     terminator      2787..3035
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     primer_bind     complement(3725..3741)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3725..3741)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3738..3760)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3749..3765
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3773..3779,3780..3797,3798..3803))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3818..3839
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        4030..4706
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     CDS             4774..5571
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLL
                     SSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEH
                     QGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDI
                     ALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4831..4850)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5441..5460
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5611..5785
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    5863..5887
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6312..7106
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      7193..7781
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7682..7701
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7935..7952
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7967..8107
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8033..8052)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8193..8215
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8451..8645
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8711..9784)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10213..10842)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    12143..12167
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     terminator      complement(12189..12441)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(12473..12490)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(join(12497..14314,14505..14519))
                     /codon_start=1
                     /product="beta-glucuronidase"
                     /label=GUSPlus(TM)
                     /note="codon-optimized Staphylococcus gusA gene with a 
                     catalase intron to ensure expression in plants but not 
                     bacteria"
                     /translation="MVDLRNRRTSLYPINTETRGVFDLNGVWNFKLDYGKGLEEKWYES
                     KLTDTISMAVPSSYNDIGVTKEIRNHIGYVWYEREFTVPAYLKDQRIVLRFGSATHKAI
                     VYVNGELVVEHKGGFLPFEAEINNSLRDGMNRVTVAVDNILDDSTLPVGLYSERHEEGL
                     GKVIRNKPNFDFFNYAGLHRPVKIYTTPFTYVEDISVVTDFNGPTGTVTYTVDFQGKAE
                     TVKVSVVDEEGKVVASTEGLSGNVEIPNVILWEPLNTYLYQIKVELVNDGLTIDVYEEP
                     FGVRTVEVNDGKFLINNKPFYFKGFGKHEDTPINGRGFNEASNVMDFNILKWIGANSFR
                     TAHYPYSEELMRLADREGLVVIDETPAVGVHLNFMATTGLGEGSERVSTWEKIRTFEHH
                     QDVLRELVSRDKNHPSVVMWSIANEAATEEEGAYEYFKPLVELTKELDPQKRPVTIVLF
                     VMATPETDKVAELIDVIALNRYNGWYFDGGDLEAAKVHLRQEFHAWNKRCPGKPIMITE
                     YGADTVAGFHDIDPVMFTEEYQVEYYQANHVVFDEFENFVGEQAWNFADFATSQGVMRV
                     QGNKKGVFTRDRKPKLAAHVFRERWTNIPDFGYKN"
     intron          14315..14504
                     /label=cat1 intron
                     /note="castor bean catalase intron, modified"
ORIGIN
        1 gtcaagagtc ccccgtgttc tctccaaatg aaatgaactt ccttatatag aggaagggtc
       61 ttgcgaagga tagtgggatt gtgcgtcatc ccttacgtca gtggagatat cacatcaatc
      121 cacttgcttt gaagacgtgg ttggaacgtc ttctttttcc acgatgctcc tcgtgggtgg
      181 gggtccatct ttgggaccac tgtcggcaga ggcatcttca acgatggcct ttcctttatc
      241 gcaatgatgg catttgtagg agccaccttc cttttccact atcttcacaa taaagtgaca
      301 gatagctggg caatggaatc cgaggaggtt tccggatatt accctttgtt gaaaagtctc
      361 aattgccctt tggtcttctg agactgtatc tttgatattt ttggagtaga caagtgtgtc
      421 gtgctccacc atgttgacga agattttctt cttgtcattg agtcgtaaga gactctgtat
      481 gaactgttcg ccagtcttta cggcgagttc tgttaggtcc tctatttgaa tctttgactc
      541 catgaagcta aactgaaggc gggaaacgac aatctgatcc aagctcaagc tgctctagca
      601 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
      661 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt
      721 ttcccagtca cgacgttgta aaacgacggc cagtgccaag cttgcatgcc tgcaggtccc
      781 cagattagcc ttttcaattt cagaaagaat gctaacccac agatggttag agaggcttac
      841 gcagcaggtc tcatcaagac gatctacccg agcaataatc tccaggaaat caaatacctt
      901 cccaagaagg ttaaagatgc agtcaaaaga ttcaggacta actgcatcaa gaacacagag
      961 aaagatatat ttctcaagat cagaagtact attccagtat ggacgattca aggcttgctt
     1021 cacaaaccaa ggcaagtaat agagattgga gtctctaaaa aggtagttcc cactgaatca
     1081 aaggccatgg agtcaaagat tcaaatagag gacctaacag aactcgccgt aaagactggc
     1141 gaacagttca tacagagtct cttacgactc aatgacaaga agaaaatctt cgtcaacatg
     1201 gtggagcacg acacacttgt ctactccaaa aatatcaaag atacagtctc agaagaccaa
     1261 agggcaattg agacttttca acaaagggta atatccggaa acctcctcgg attccattgc
     1321 ccagctatct gtcactttat tgtgaagata gtggaaaagg aaggtggctc ctacaaatgc
     1381 catcattgcg ataaaggaaa ggccatcgtt gaagatgcct ctgccgacag tggtcccaaa
     1441 gatggacccc cacccacgag gagcatcgtg gaaaaagaag acgttccaac cacgtcttca
     1501 aagcaagtgg attgatgtga tatctccact gacgtaaggg atgacgcaca atcccactat
     1561 ccttcgcaag acccttcctc tatataagga agttcatttc atttggagag aacacggggg
     1621 actcaaacat tactcattca caaaaccatc ttaaagcaac tacacaagtc ttgaaatttt
     1681 ctcatatttt ctatttacta tataaacttt taatcaaatc aagattaaag ttaattaaat
     1741 gacaaccttc aaaatcgagt cccggatcca cggcaacctc aacggggaga agttcgagtt
     1801 ggttggaggt ggagtaggtg aggagggtcg cctcgagatt gagatgaaga ctaaagataa
     1861 accactggca ttctctccct tcctgctgac cacttgcatg ggttacgggt tctaccactt
     1921 cgccagcttc ccaaagggga ttaagaacat ctataaaagt tatcaggcat gcacctggta
     1981 gctagtcttt aaaccaatag attgcatcgg tttaaaaggc aagaccgtca aattgcggga
     2041 aaggggtcaa cagccgttca gtaccaagtc tcaggggaaa ctttgagatg gccttgcaaa
     2101 gggtatggta ataagctgac ggacatggtc ctaaccacgc agccaagtcc taagtcaaca
     2161 gatcttctgt tgatatggat gcagttcaca aactaaatgt cggtcgggga agatgtattc
     2221 ttctcataag atatagtcgg acctctcctt aatgggagct agcggatgaa gtgatgcaac
     2281 actggagccg ctgggaacta atttgtatgc gaaagtatat tgattagttt tggagtactc
     2341 gcttcatgct gcaacgaacg gaggttacac caacaccagg aaggagatct atgaagacgg
     2401 cggcatcttg gaggtcaact tccgttacac ttacgagttc aacaagatca tcggtgacgt
     2461 cgagtgcatt ggacatggat tcccaagtca gagtccgatc ttcaaggaca cgatcgtgaa
     2521 gagttgtccc acggtggacc tgatgttgcc aatgtccggg aacatcatcg ccagctccta
     2581 cgcttacgcc ttccaactga aggacggctc tttctacacg gcagaagtca agaacaacat
     2641 agacttcaag aatccaatcc acgagtcctt ctcgaagtca gggcccatgt tcacccacag
     2701 acgtgtcgag gagactctca ccaaggagaa ccttgccata gtggagtacc agcaggtttt
     2761 caacagcgcc ccaagagaca tgtagatatg aagatgaaga tgaaatattt ggtgtgtcaa
     2821 ataaaaagct tgtgtgctta agtttgtgtt tttttcttgg cttgttgtgt tatgaatttg
     2881 tggctttttc taatattaaa tgaatgtaag atctcattat aatgaataaa caaatgtttc
     2941 tataatccat tgtgaatgtt ttgttggatc tcttctgcag catataacta ctgtatgtgc
     3001 tatggtatgg actatggaat atgattaaag ataagatggg ctcatagagt aaaacgaggc
     3061 gagggaccta taaacctccc ttcatcatgc tatttcatga tctattttat aaaataaaga
     3121 tgtagaaaaa agtaagcgta ataaccgcaa aacaaatgat ttaaaacatg gcacataatg
     3181 aggagattaa gttcggttta cgtttatttt agtactaatt gtaacgtgag actacgtatc
     3241 gggaatcgcc taattaaagc attaatgcga acctgattag attcaccgac cctcctatcg
     3301 tgtcgacctt tctgtttctt agaatttttt ggtagtctat gtactaataa tgtcagcttc
     3361 gtatttattt cataagcaat ttgcatttgc aatttgtttt ttacttttat ttttattgta
     3421 ttgtggaatg tggactcgta ccaacatgaa gttatatacc accaaaaaaa ttacagttag
     3481 tcaaaagatt cacgagtgag agctacttat gattgtcttt tacgtatatg tctaattgtc
     3541 tatttgctca ataatctttg tactttcttt tgtcgttgat aaaatcacaa agttccaaaa
     3601 gtaatcgaat gatttgcttt taagaaaaga agagctcaat aattcaacat atatctgtac
     3661 acaattaccg cctttgagtg agctctagat gccacctgac gtctaagaag aattcgtaat
     3721 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     3781 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     3841 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     3901 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg ctagagcagc ttgccaacat
     3961 ggtggagcac gacactctcg tctactccaa gaatatcaaa gatacagtct cagaagacca
     4021 aagggctatt gagacttttc aacaaagggt aatatcggga aacctcctcg gattccattg
     4081 cccagctatc tgtcacttca tcaaaaggac agtagaaaag gaaggtggca cctacaaatg
     4141 ccatcattgc gataaaggaa aggctatcgt tcaagatgcc tctgccgaca gtggtcccaa
     4201 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc
     4261 aaagcaagtg gattgatgtg aacatggtgg agcacgacac tctcgtctac tccaagaata
     4321 tcaaagatac agtctcagaa gaccaaaggg ctattgagac ttttcaacaa agggtaatat
     4381 cgggaaacct cctcggattc cattgcccag ctatctgtca cttcatcaaa aggacagtag
     4441 aaaaggaagg tggcacctac aaatgccatc attgcgataa aggaaaggct atcgttcaag
     4501 atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc atcgtggaaa
     4561 aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgatatc tccactgacg
     4621 taagggatga cgcacaatcc cactatcctt cgcaagaccc ttcctctata taaggaagtt
     4681 catttcattt ggagaggaca cgctgaaatc accagtctct ctctacaaat ctatctctct
     4741 cgagctttcg cagatcccgg ggggcaatga gatatgggga ttgaacaaga tggattgcac
     4801 gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca
     4861 atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt
     4921 gtcaagaccg acctgtccgg tgccctgaat gaactccagg acgaggcagc gcggctatcg
     4981 tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga
     5041 agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct
     5101 cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg
     5161 gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg
     5221 gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc
     5281 gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacacat
     5341 ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac
     5401 tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt
     5461 gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct
     5521 cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg aagtagatgc
     5581 cgaccggatc tgtcgatcga caagctcgag tttctccata ataatgtgtg agtagttccc
     5641 agataaggga attagggttc ctatagggtt tcgctcatgt gttgagcata taagaaaccc
     5701 ttagtatgta tttgtatttg taaaatactt ctatcaataa aatttctaat tcctaaaacc
     5761 aaaatccagt actaaaatcc agatcccccg aattaattcg gcgttaattc agtacattaa
     5821 aaacgtccgc aatgtgttat taagttgtct aagcgtcaat ttgtttacac cacaatatat
     5881 cctgccacca gccagccaac agctccccga ccggcagctc ggcacaaaat caccactcga
     5941 tacaggcagc ccatcagtcc gggacggcgt cagcgggaga gccgttgtaa ggcggcagac
     6001 tttgctcatg ttaccgatgc tattcggaag aacggcaact aagctgccgg gtttgaaaca
     6061 cggatgatct cgcggagggt agcatgttga ttgtaacgat gacagagcgt tgctgcctgt
     6121 gatcaccgcg gtttcaaaat cggctccgtc gatactatgt tatacgccaa ctttgaaaac
     6181 aactttgaaa aagctgtttt ctggtattta aggttttaga atgcaaggaa cagtgaattg
     6241 gagttcgtct tgttataatt agcttcttgg ggtatcttta aatactgtag aaaagaggaa
     6301 ggaaataata aatggctaaa atgagaatat caccggaatt gaaaaaactg atcgaaaaat
     6361 accgctgcgt aaaagatacg gaaggaatgt ctcctgctaa ggtatataag ctggtgggag
     6421 aaaatgaaaa cctatattta aaaatgacgg acagccggta taaagggacc acctatgatg
     6481 tggaacggga aaaggacatg atgctatggc tggaaggaaa gctgcctgtt ccaaaggtcc
     6541 tgcactttga acggcatgat ggctggagca atctgctcat gagtgaggcc gatggcgtcc
     6601 tttgctcgga agagtatgaa gatgaacaaa gccctgaaaa gattatcgag ctgtatgcgg
     6661 agtgcatcag gctctttcac tccatcgaca tatcggattg tccctatacg aatagcttag
     6721 acagccgctt agccgaattg gattacttac tgaataacga tctggccgat gtggattgcg
     6781 aaaactggga agaagacact ccatttaaag atccgcgcga gctgtatgat tttttaaaga
     6841 cggaaaagcc cgaagaggaa cttgtctttt cccacggcga cctgggagac agcaacatct
     6901 ttgtgaaaga tggcaaagta agtggcttta ttgatcttgg gagaagcggc agggcggaca
     6961 agtggtatga cattgccttc tgcgtccggt cgatcaggga ggatatcggg gaagaacagt
     7021 atgtcgagct attttttgac ttactgggga tcaagcctga ttgggagaaa ataaaatatt
     7081 atattttact ggatgaattg ttttagtacc tagaatgcat gaccaaaatc ccttaacgtg
     7141 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     7201 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     7261 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     7321 cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact
     7381 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     7441 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     7501 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     7561 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     7621 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     7681 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     7741 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     7801 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     7861 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     7921 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt
     7981 ttctccttac gcatctgtgc ggtatttcac accgcatatg gtgcactctc agtacaatct
     8041 gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat
     8101 ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
     8161 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
     8221 accgtcatca ccgaaacgcg cgaggcaggg tgccttgatg tgggcgccgg cggtcgagtg
     8281 gcgacggcgc ggcttgtccg cgccctggta gattgcctgg ccgtaggcca gccatttttg
     8341 agcggccagc ggccgcgata ggccgacgcg aagcggcggg gcgtagggag cgcagcgacc
     8401 gaagggtagg cgctttttgc agctcttcgg ctgtgcgctg gccagacagt tatgcacagg
     8461 ccaggcgggt tttaagagtt ttaataagtt ttaaagagtt ttaggcggaa aaatcgcctt
     8521 ttttctcttt tatatcagtc acttacatgt gtgaccggtt cccaatgtac ggctttgggt
     8581 tcccaatgta cgggttccgg ttcccaatgt acggctttgg gttcccaatg tacgtgctat
     8641 ccacaggaaa gagacctttt cgaccttttt cccctgctag ggcaatttgc cctagcatct
     8701 gctccgtaca ttaggaaccg gcggatgctt cgccctcgat caggttgcgg tagcgcatga
     8761 ctaggatcgg gccagcctgc cccgcctcct ccttcaaatc gtactccggc aggtcatttg
     8821 acccgatcag cttgcgcacg gtgaaacaga acttcttgaa ctctccggcg ctgccactgc
     8881 gttcgtagat cgtcttgaac aaccatctgg cttctgcctt gcctgcggcg cggcgtgcca
     8941 ggcggtagag aaaacggccg atgccgggat cgatcaaaaa gtaatcgggg tgaaccgtca
     9001 gcacgtccgg gttcttgcct tctgtgatct cgcggtacat ccaatcagct agctcgatct
     9061 cgatgtactc cggccgcccg gtttcgctct ttacgatctt gtagcggcta atcaaggctt
     9121 caccctcgga taccgtcacc aggcggccgt tcttggcctt cttcgtacgc tgcatggcaa
     9181 cgtgcgtggt gtttaaccga atgcaggttt ctaccaggtc gtctttctgc tttccgccat
     9241 cggctcgccg gcagaacttg agtacgtccg caacgtgtgg acggaacacg cggccgggct
     9301 tgtctccctt cccttcccgg tatcggttca tggattcggt tagatgggaa accgccatca
     9361 gtaccaggtc gtaatcccac acactggcca tgccggccgg ccctgcggaa acctctacgt
     9421 gcccgtctgg aagctcgtag cggatcacct cgccagctcg tcggtcacgc ttcgacagac
     9481 ggaaaacggc cacgtccatg atgctgcgac tatcgcgggt gcccacgtca tagagcatcg
     9541 gaacgaaaaa atctggttgc tcgtcgccct tgggcggctt cctaatcgac ggcgcaccgg
     9601 ctgccggcgg ttgccgggat tctttgcgga ttcgatcagc ggccgcttgc cacgattcac
     9661 cggggcgtgc ttctgcctcg atgcgttgcc gctgggcggc ctgcgcggcc ttcaacttct
     9721 ccaccaggtc atcacccagc gccgcgccga tttgtaccgg gccggatggt ttgcgaccgc
     9781 tcacgccgat tcctcgggct tgggggttcc agtgccattg cagggccggc aggcaaccca
     9841 gccgcttacg cctggccaac cgcccgttcc tccacacatg gggcattcca cggcgtcggt
     9901 gcctggttgt tcttgatttt ccatgccgcc tcctttagcc gctaaaattc atctactcat
     9961 ttattcattt gctcatttac tctggtagct gcgcgatgta ttcagatagc agctcggtaa
    10021 tggtcttgcc ttggcgtacc gcgtacatct tcagcttggt gtgatcctcc gccggcaact
    10081 gaaagttgac ccgcttcatg gctggcgtgt ctgccaggct ggccaacgtt gcagccttgc
    10141 tgctgcgtgc gctcggacgg ccggcactta gcgtgtttgt gcttttgctc attttctctt
    10201 tacctcatta actcaaatga gttttgattt aatttcagcg gccagcgcct ggacctcgcg
    10261 ggcagcgtcg ccctcgggtt ctgattcaag aacggttgtg ccggcggcgg cagtgcctgg
    10321 gtagctcacg cgctgcgtga tacgggactc aagaatgggc agctcgtacc cggccagcgc
    10381 ctcggcaacc tcaccgccga tgcgcgtgcc tttgatcgcc cgcgacacga caaaggccgc
    10441 ttgtagcctt ccatccgtga cctcaatgcg ctgcttaacc agctccacca ggtcggcggt
    10501 ggcccatatg tcgtaagggc ttggctgcac cggaatcagc acgaagtcgg ctgccttgat
    10561 cgcggacaca gccaagtccg ccgcctgggg cgctccgtcg atcactacga agtcgcgccg
    10621 gccgatggcc ttcacgtcgc ggtcaatcgt cgggcggtcg atgccgacaa cggttagcgg
    10681 ttgatcttcc cgcacggccg cccaatcgcg ggcactgccc tggggatcgg aatcgactaa
    10741 cagaacatcg gccccggcga gttgcagggc gcgggctaga tgggttgcga tggtcgtctt
    10801 gcctgacccg cctttctggt taagtacagc gataaccttc atgcgttccc cttgcgtatt
    10861 tgtttattta ctcatcgcat catatacgca gcgaccgcat gacgcaagct gttttactca
    10921 aatacacatc acctttttag acggcggcgc tcggtttctt cagcggccaa gctggccggc
    10981 caggccgcca gcttggcatc agacaaaccg gccaggattt catgcagccg cacggttgag
    11041 acgtgcgcgg gcggctcgaa cacgtacccg gccgcgatca tctccgcctc gatctcttcg
    11101 gtaatgaaaa acggttcgtc ctggccgtcc tggtgcggtt tcatgcttgt tcctcttggc
    11161 gttcattctc ggcggccgcc agggcgtcgg cctcggtcaa tgcgtcctca cggaaggcac
    11221 cgcgccgcct ggcctcggtg ggcgtcactt cctcgctgcg ctcaagtgcg cggtacaggg
    11281 tcgagcgatg cacgccaagc agtgcagccg cctctttcac ggtgcggcct tcctggtcga
    11341 tcagctcgcg ggcgtgcgcg atctgtgccg gggtgagggt agggcggggg ccaaacttca
    11401 cgcctcgggc cttggcggcc tcgcgcccgc tccgggtgcg gtcgatgatt agggaacgct
    11461 cgaactcggc aatgccggcg aacacggtca acaccatgcg gccggccggc gtggtggtgt
    11521 cggcccacgg ctctgccagg ctacgcaggc ccgcgccggc ctcctggatg cgctcggcaa
    11581 tgtccagtag gtcgcgggtg ctgcgggcca ggcggtctag cctggtcact gtcacaacgt
    11641 cgccagggcg taggtggtca agcatcctgg ccagctccgg gcggtcgcgc ctggtgccgg
    11701 tgatcttctc ggaaaacagc ttggtgcagc cggccgcgtg cagttcggcc cgttggttgg
    11761 tcaagtcctg gtcgtcggtg ctgacgcggg catagcccag caggccagcg gcggcgctct
    11821 tgttcatggc gtaatgtctc cggttctagt cgcaagtatt ctactttatg cgactaaaac
    11881 acgcgacaag aaaacgccag gaaaagggca gggcggcagc ctgtcgcgta acttaggact
    11941 tgtgcgacat gtcgttttca gaagacggct gcactgaacg tcagaagccg actgcactat
    12001 agcagcggag gggttggatc aaagtacttt gatcccgagg ggaaccctgt ggttggcatg
    12061 cacatacaaa tggacgaacg gataaacctt ttcacgccct tttaaatatc cgattattct
    12121 aataaacgct cttttctctt aggtttaccc gccaatatat cctgtcaaac actgatagtt
    12181 taattcccga tctagtaaca tagatgacac cgcgcgcgat aatttatcct agtttgcgcg
    12241 ctatattttg ttttctatcg cgtattaaat gtataattgc gggactctaa tcataaaaac
    12301 ccatctcata aataacgtca tgcattacat gttaattatt acatgcttaa cgtaattcaa
    12361 cagaaattat atgataatca tcgcaagacc ggcaacagga ttcaatctta agaaacttta
    12421 ttgccaaatg tttgaacgat cggggaaatt cgagctggtc accaattcac acgtgatggt
    12481 gatggtgatg gctagcgttc ttgtagccga aatctggaat gttggtccag cgctcgcgaa
    12541 agacgtgcgc ggcgagcttc ggcttgcggt cacgagtgaa cacgcccttc ttgtttcctt
    12601 ggacgcgcat cacgccctga gaggtcgcga agtccgcgaa gttccacgct tgctcaccca
    12661 cgaagttctc aaactcatcg aacacgacgt ggttcgcctg gtagtactcg acttgatatt
    12721 cctcggtgaa catcactgga tcaatgtcgt gaaagcccgc aacggtgtct gcgccgtact
    12781 cagtgatcat gatcggcttt cctgggcaac gcttgttcca cgcgtgaaat tcctggcgga
    12841 gatggacttt ggccgcttcg agatcaccgc catcgaagta ccatccgtta tagcgattga
    12901 gcgcgatgac gtcaatcagt tcggcgactt tgtccgtctc cggggtagcc atcacaaaca
    12961 gcacgatcgt gaccggacgc ttctgtgggt cgagttcctt ggtcagctcc accaacggct
    13021 tgaagtactc gtacgcgccc tcttcctcag tcgccgcctc gttggcgatg ctccacatca
    13081 cgacgcttgg atggttcttg tcacgagaca ccagttcacg gagaacgtct tgatggtgct
    13141 caaacgtccg aatcttctcc caggtactga cgcgctcgct gccttcgccg agtcccgtgg
    13201 tggccatgaa gttgaggtgc acgccaactg ccggagtctc gtcgatcacg accagaccct
    13261 cgcgatccgc aagacgcatc aactcttcag agtacggata gtgtgcggtc cggaagctgt
    13321 tggcgccgat ccatttgagg atattgaaat ccatcacatt gctcgcttcg ttaaagccac
    13381 ggccgttgat aggagtgtcc tcatgtttgc caaagccctt gaagtagaac ggtttgttgt
    13441 tgatgaggaa cttgccgtcg ttgacttcca cggtccgcac gccgaacggc tcttcataga
    13501 catcgatggt cagtccgtcg ttcaccagtt ccactttgat ctggtagaga tacgtgttca
    13561 gtggttccca gaggatgaca ttcggaatct ccacgttacc gctcaggccc tcggtgcttg
    13621 cgaccacttt gccttcctca tccacgaccg acactttcac ggtctcggct ttgccttgaa
    13681 agtccaccgt ataggtcaca gtcccggttg ggccattgaa gtcggtcaca accgagatgt
    13741 cctcgacgta cgtaaacggg gtcgtgtaga ttttcaccgg acggtgcagg cctgcatagt
    13801 tgaagaagtc gaagttcggc ttgttacgaa tgacttttcc gaggccctct tcgtggcgct
    13861 cgctgtacag ccccaccggg agggtgctat cgtcgaggat gttgtccacg gcgacggtga
    13921 cgcgattcat gccatcacgc agcgagttgt tgatttccgc ttcgaatggc aggaatccgc
    13981 ccttgtgctc cacgaccagc tcaccattga catagacaat tgctttgtga gttgcagagc
    14041 cgaagcggag cacgatacgc tgatccttca gataggccgg caccgtgaac tcacgttcgt
    14101 accagacata tccgatatgg ttgcggattt ccttggtcac gccaatgtca ttgtaactgc
    14161 ttgggacggc catactaata gtgtcggtca gcttgctttc gtaccacttc tcttccagtc
    14221 ctttcccgta gtccagcttg aagttccaga cgccattgag gtcgaagacg ccacgggtct
    14281 cggtgttgat cgggtacaga ctagttcgtc ggttctgtaa ctatcatcat catcatagac
    14341 acacgaaata aagtaatcag attatcagtt aaagctatgt aatatttaca ccataaccaa
    14401 tcaattaaaa aatagatcag tttaaagaaa gatcaaagct caaaaaaata aaaagagaaa
    14461 agggtcctaa ccaagaaaat gaaggagaaa aactagaaat ttaccctcag atctaccatg
//
