LOCUS       sequence-476852-       15803 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This all-in-one vector is used to overexpress the GhSFT gene via 
            virus-mediated overexpression (VOX), and specifically silence the 
            GhSP gene via virus-induced gene silencing (VIGS), simultaneously..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15803)
  AUTHORS   Hao Y, Yuan Z, Li Y, Zuo D, Cheng H, Wang Q, Zhang Y, Lv L, Liu J, 
            Song G
  TITLE     An all-in-one plant virus-based vector toolkit for streamlined gene 
            silencing, overexpression, and genome editing.
  JOURNAL   Plant Commun. 2025 May 19:101379. doi: 10.1016/j.xplc.2025.101379.
  PUBMED    40394900
REFERENCE   2  (bases 1 to 15803)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Commun. 2025 May 19:101379. doi: 10.1016/j.xplc.2025.101379."
COMMENT     Sequence Label: pVM-SFT-SP
FEATURES             Location/Qualifiers
     source          1..15803
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(8889..8906)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8889..8905)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8898..8920)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    9108..9132
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             10433..11062
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             11491..12564
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      12630..12824
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(13060..13082)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    13168..13308
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     13223..13242
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(13323..13340)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(13494..14082)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13574..13593)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(14169..14963)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    15388..15412
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     protein_bind    15680..15701
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(15716..15721,15722..15739,15740..15746)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    15754..15770
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     15759..15781
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     15778..15794
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     15778..15794
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aattcgagct cattcgagct ccagaacgat ctcagttagg tcatgggaaa gctgatattc
       61 atccctctga gactctatat aattaaatgc gtttggggga ttagctaatt gagaatccat
      121 atagagaaat atggccgcgc agcgacgccg gttgaagaat atgaacaagg aataagatga
      181 acaaggaaga agatgaacaa gcaataattt aattaattat tgttaataag cataaattat
      241 gaaaataatt ttgatggtaa tgaaagtatt ataaataata tttaggagaa aatattgtta
      301 aggaaatatc tggttatgtt tatttgttaa ggtatatggg tttttataga aacccaggag
      361 cagctatggt aagaaaccca ggactcaaaa aatgcctctc aacttctctc atatgttgcg
      421 gagtctggag tcccatttat accatagaaa gcattggggg tatagcatgg taatttcaca
      481 tcggactcca taccgcggcc atctatataa tattactgat ggccgcgcga agtcccggta
      541 tgccttttca gccgttggat ctggagccgt acctttttcg cgttcccatt ttgcccctgg
      601 agtcccgagc tatttacgac cccctgccac cgagtcccct tcgtggagcg ctcttcgtcc
      661 aacacgtgtc ccaacttttg cgcatttttg actacctgtc gcgcacttga attaaagttg
      721 cccttttatt ttcttccaat tattttgcgg ctgagtagtc tagataattg tgacaactta
      781 gtgagcaagt tgtaggagga ccctatccgt ctgggaaaat aattgggttt tcacgtgtac
      841 caatgggaaa taatatgtat agcctattta atttagaaaa ttaaatagtt ttagtaattc
      901 ctatataata gcgtttacta gtctgggtaa aattcgtata ggaaaaaata tttattattt
      961 tttaaattta acctattgaa aatgtacagt ttaaagagta gacgtggatc gaattacatt
     1021 caagcacgtg gaaactcacg gaccccatta ttcaaacgac attccactgg taagcgttat
     1081 ggaggaagac gactacagcc actgaatgtg aaaagagaag atgaacccag aatgtcgacc
     1141 caacgcatcc acgagaatca atatgggtcg gaatttacaa tggcccacaa cacagccatt
     1201 tcgacctata tctcctatcc taatctgggc aagactgaac ccaatcgaac caggtcgtac
     1261 atcaagctga aacgattgcg tttcaagggc acggtgaaga tcgaacgagt tcctactgac
     1321 gtggctatgg atggttcgat tcccaacacg gaaggggtgt tctcattggt catagtcgtt
     1381 gaccgaaaac ctcatttgac tgcatctgga tgcctacata cgtttgacga actatttggt
     1441 gcgataatcc acagccatgg aaccctgtct atagttcccg cgttgaagga tcgttactac
     1501 atacggcatg taattaaacg ggtactgtcc gtcgagaaag ataacgtcat ggtggatatt
     1561 gaaggatcca ctgctctgtc taataggcgt tttaattgtt gggccagttt taaagatctt
     1621 gatttggatt catgtaaggg tgtttatgac aacataaaca agaatgccct gttagtttat
     1681 tactcatggg tatcggatac tgtgtctaaa gcatccactt atgtatcgta tgaccttgat
     1741 tatattggat aatcaataat aacaggaaaa tttgaataag aaaaaatatt atttaaatat
     1801 ttttggttga ggaccattac aactactctt gatacattct tggaccgttg tcctaaccag
     1861 ttcgtttaat tgggccatag acatggtaat gttggactca gccctatggg cccctactat
     1921 tgatgccgag tcacctggat ccagtacagt ggaccctaac ctgtgcagct ctctgtaagg
     1981 tgtctgttcg ttattggact gtgagtccgc atcttcgtta acgggcccta tggtactcct
     2041 ggtcgcccac gactcccctg gccttatttc tattgggcct ggaagcccaa atctgttcat
     2101 ggatgcggac ctaattagtt tcctttccca tttaccatag tctacatgtg agaaatcaac
     2161 gtctttagcg gagaactgtt tggaaaggat ctttaccgtt ggcgccttga acggaatatc
     2221 gacggaatgt ttggcggtcg ataatttcag cttccctttg aattttgcga agtgggtacg
     2281 ctgttgaaca ttcgtatcag aaactctgta atagagtttc caagggattg ggtctttgag
     2341 agagaagaag gaagctgaaa aatagtggag atctatgtta cacctgatcg gaaaagtcca
     2401 cgacgattgt aatgattcat tatcagtcat cctcttgtcg tgaatctcca ctatcactgt
     2461 accagtcgcg ttaattggca cctgctgcct gtactcgata acgcaatgat cgatcttcat
     2521 gcaactgcga ctgagtctcg ccgttaattg tgccgccgtt gacggaaact gcagaacgat
     2581 ctcagttagg tcatgggaaa gctgatattc atccctctga gactctatat aattaaatgc
     2641 gtttggggga ttagctaatt gagaatccat atagagaaat atggccgcgc agcgacgccg
     2701 gttgaagaat atgaacaagg aataagatga acaaggaaga agatgaacaa gcaataattt
     2761 aattaattat tgttaataag cataaattat gaaaataatt ttgatggtaa tgaaagtatt
     2821 ataaataata tttaggagaa aatattgtta aggaaatatc tggttatgtt tatttgttaa
     2881 ggtatatggg tttttataga aacccaggag cagctatggt aagaaaccca ggactcaaaa
     2941 aatgcctctc aacttctctc atatgttgcg gagtctggag tcccatttat accatagaaa
     3001 gcattggggg tatagcatgg taatttcaca tcggactcca taccgcggcc atctatataa
     3061 tattactgat ggccgcgcga agtcccggta tgccttttca gccgttggat ctggagccgt
     3121 acctttttcg cgttcccatt ttgcccctgg agtcccgagc tatttacgac cccctgccac
     3181 cgagtcccct tcgtggagcg ctcttcgtcc aacacgtgtc ccaacttttg cgcatttttg
     3241 actacctgtc gcgcacttga attaaagttg cccttttatt ttcttccaat tattttgcgg
     3301 ctgagtagtc tagtggatcc cccggagctc cacttgggat aggttaagaa aatatgtctg
     3361 gcttgtaaac gaaaagaatc ggggtttcta ggcatttttt aaatagtgac ccaggactca
     3421 aaaactagct ctcaacttct ctcatatgtt gtggagtctg gagtccttta tatatgttta
     3481 cgctatagaa ggcatttggg gactctcccg ggcccaccat aggactccat accgcggcca
     3541 tctatataat attactgatg gccgcgcgga ttttggagcc cccttttgtc gttagcgcga
     3601 cctttaattc aaattaaagg ttgctttttt ctatcgtccg atcattttgc gcctgactag
     3661 cctagatatt agcaacaact tcgcggctaa gttgttgacc gtcaagtata aattaatggg
     3721 cttatggcct aatgggtata gagcaaaatg gcatgcctgc agactagtgc gtcttctagc
     3781 agctgtttcc ctttgagcat tgaaatagac agctgcaaca ggaaccccta gttcgttttc
     3841 ttctgcaaac ttcctggtat cgaaacgatc ccttgatgac ggtatgcttc tcactgtttg
     3901 cctgcctttt tgcttgaaga ggaggaacac aaacctgtgg atccctatgt ttggccttgg
     3961 catttcgtag ttcaccattt cccttccaaa tgtggcatct gttgtgccgg ggatatctgt
     4021 cactatccag tgtaagtgct ccctcaggta agggtcacta ggaccaggaa catctgggtc
     4081 tgtcatcacc agggtgaaaa aggatctcat atcacctcca tgaacctcaa ccttaggctt
     4141 gttagtaact gcagatggaa aaaattcatg gccattatat acctgcttgt tggtgttgaa
     4201 agtgactgac attttcacag atggggagag ggcatcaata acatccccaa tcactcattc
     4261 gctagcgtta actggccata atcctgtgta tgcaacgttg aaaattcgga tctattttta
     4321 cgattcgatc atgaattaat aaaatttaaa ttttattgaa tgattttcta gtacataact
     4381 gacatacgac ttatcagttg cgaaacgaac agctctaatt acattgttaa gtgaaattac
     4441 gcctaattcg tctaagtaca acataaccaa ctgtttaaac ctaattaaat aagtcgaccc
     4501 agaagctgtc agagatgtcg tccagacttg gaagttcagg taagctttgt ggagatgcaa
     4561 cgctctccgt aagttgtggt tgaatcggat ctggacgtgg tatatacgcg tcctggtgta
     4621 tagcagatcc tctactccgt atatcttgaa atatagggga tttattatct cccagatata
     4681 cacgccattc tctgcctgac gtgcagtgat gatttcccct gtgcgtgaat ccatgaccgg
     4741 tgcagcctat gtggaagtat atggtgcacc cacagtttag atcaatgcgt cgtcgtctaa
     4801 tcgccctcct cttggcttgc ctgtgtgcct tcttgataga ggggggcgtc gagagtgatg
     4861 aagataacat tcttgagagt ccaatgtttg agcgacgcat tttccgcttt atccaggaaa
     4921 tctttatagc tggatccctc gcctggattg caaagcacga ttgaagggat ccctccttta
     4981 atttgaactg gtttcccgta cttgcaattt gactgccagt ctttttgggc cccaagaagc
     5041 tctttccaat gctttagctt taggtattgc ggtgctacgt catcaatgac gttataggca
     5101 acattattcg agtacactcg attattgaag tccaggtgtc cactgaggta attatgtggg
     5161 cctaaagcac gagcccacat cgtcttgccc gttctagaat caccttccac tatgagacta
     5221 actggtctta caggccgcgc agcgggaccc attccaaaat aatcatccgc ccattcttgc
     5281 atctgttcag gaacgttagt gaaagaggaa agtggaaatg gagggaccca ttgttcaggc
     5341 gcctttttga aaaggcgttc caagttcgct gtaatgttat ggtaactcac gatgaacgcc
     5401 cttggatctc cggcccggat aattgcaaga gcttccgcca aactaggtgc attgatggcg
     5461 ttataataga cgtcgtcttt attcttcttt gtacccccag gcactttgaa ctgtccggat
     5521 tcacaacaat cgccgtcttt ggtgatgtaa ttcttgacgg cgttggcgtc tttggctgct
     5581 tgtatgtttg ggtgaaaatt ggtcgacctt ctggggtgag taaggtcgaa aaatctctta
     5641 ttcttgatgt tggactttcc agagagttgg atgagacagt gaaggtgtgg gaatccgtcc
     5701 gagtgctcct ctctggcaac tcgaatatat gttggtttga cgactgacca tgataaggtt
     5761 tgaagcattt gaagagcttc attttttggg atatcgcact tgggataggt taagaaaata
     5821 tgtctggctt gtaaacgaaa agaatcgggg tttctaggca ttttttaaat agtgacccag
     5881 gactcaaaaa ctagctctca acttctctca tatgttgtgg agtctggagt cctttatata
     5941 tgtttacgct atagaaggca tttggggact ctcccgggcc caccatagga ctccataccg
     6001 cggccatcta tataatatta ctgatggccg cgcggatttt ggagccccct tttgtcgtta
     6061 gcgcgacctt taattcaaat taaaggttgc ttttttctat cgtccaatca ttttgcgcct
     6121 gactagccta gatattagca acaacttcgc ggctaagttg ttgaccgtca agtataaatt
     6181 aatgggctta tggcctaatg ggtatagagc aaaatggcat gcctgcagac tagtatgcct
     6241 agagatagag atcctttggt tgttggtagg gttataggag atgtgttgga cccttttaca
     6301 aggtctattt cacttagggt cacttatgct actagggatg ttaacaatgg ggttgagctt
     6361 aaaccatctc aagttgttaa ccaaccaagg gttgatattg gtggggatga tctgaggacc
     6421 ttctacacct tggttatggt agatcctgat gctccaagtc caagtgaccc aaacctcagg
     6481 gaatacttgc actggttggt tactgatatt ccagccacaa ctggtgcaag ctttggtcaa
     6541 gaggtggtct gctacgagag cccacgacca acggtcggta tccatcgttt cgtgttcgtg
     6601 ttgttccggc aacttggaag gcaaacggtg tacgctccag ggtggcgcca aaacttcaac
     6661 actagggact ttgctgagct ttacaatctc gggttgccag tggccgcggt ttactttaac
     6721 tgccagaggg agagtggatc cggtggccgt aggacatgaa ttcgctagcg ttaactggcc
     6781 ataatcctgt gtatgcaacg ttgaaaattc ggatctattt ttacgattcg atcatgaatt
     6841 aataaaattt aaattttatt gaatgatttt ctagtacata actgacatac gacttatcag
     6901 ttgcgaaacg aacagctcta attacattgt taagtgaaat tacgcctaat tcgtctaagt
     6961 acaacataac caactgttta aacctaatta aataagtcga cccagaagct gtcagagatg
     7021 tcgtccagac ttggaagttc aggtaagctt tgtggagatg caacgctctc cgtaagttgt
     7081 ggttgaatcg gatctggacg tggtatatac gcgtcctggt gtatagcaga tcctctactc
     7141 cgtatatctt gaaatatagg ggatttatta tctcccagat atacacgcca ttctctgcct
     7201 gacgtgcagt gatgatttcc cctgtgcgtg aatccatgac cggtgcagcc tatgtggaag
     7261 tatatggtgc acccacagtt tagatcaatg cgtcgtcgtc taatcgccct cctcttggct
     7321 tgcctgtgtg ccttcttgat agaggggggc gtcgagagtg atgaagataa cattcttgag
     7381 agtccaatgt ttgagcgacg cattttccgc tttatccagg aaatctttat agctggatcc
     7441 ctcgcctgga ttgcaaagca cgattgaagg gatccctcct ttaatttgaa ctggtttccc
     7501 gtacttgcaa tttgactgcc agtctttttg ggccccaaga agctctttcc aatgctttag
     7561 ctttaggtat tgcggtgcta cgtcatcaat gacgttatag gcaacattat tcgagtacac
     7621 tcgattattg aagtccaggt gtccactgag gtaattatgt gggcctaaag cacgagccca
     7681 catcgtcttg cccgttctag aatcaccttc cactatgaga ctaactggtc ttacaggccg
     7741 cgcagcggga cccattccaa aataatcatc cgcccattct tgcatctgtt caggaacgtt
     7801 agtgaaagag gaaagtggaa atggagggac ccattgttca ggcgcctttt tgaaaaggcg
     7861 ttccaagttc gctgtaatgt tatggtaact cacgatgaac gcccttggat ctccggcccg
     7921 gataattgca agagcttccg ccaaactagg tgcattgatg gcgttataat agacgtcgtc
     7981 tttattcttc tttgtacccc caggcacttt gaactgtccg gattcacaac aatcgccgtc
     8041 tttggtgatg taattcttga cggcgttggc gtctttggct gcttgtatgt ttgggtgaaa
     8101 attggtcgac cttctggggt gagtaaggtc gaaaaatctc ttattcttga tgttggactt
     8161 tccagagagt tggatgagac agtgaaggtg tgggaatccg tccgagtgct cctctctggc
     8221 aactcgaata tatgttggtt tgacgactga ccatgataag gtttgaagca tttgaagagc
     8281 ttcatttttt gggatatcgc acttgggata ggttaagaaa atatgtctgg cttgtaaacg
     8341 aaaagaatcg gggtttctag gcatttttta aatagtgacc caggactcaa aaactagctc
     8401 tcaacttctc tcatatgttg tggagtctgg agtcctttat atatgtttac gctatagaag
     8461 gcatttgggg actctcccgg gcccaccata ggactccata ccgcggccat ctatataata
     8521 ttactgatgg ccgcgcggat tttggagccc ccttttgtcg ttagcgcgac ctttaattca
     8581 aattaaaggt tgcttttttc tatcgtccaa tcattttgcg cctgactagc ctagatatta
     8641 gcaacaactt cgcgactaag ttgttgaccg tcaagtataa attaatgggc ttatggccca
     8701 atgggtatag agcaaaatgc ccaagcgtga tgccccgtgg cgcttattag cgggtacgtc
     8761 taaggtaagt cgaaatgcca actactctcc cttgagagta ttgggcccta aagttaataa
     8821 gggacctcga gggggggccc ggtacccggg gatcctctag agtcgacctg caggcatgca
     8881 agcttggcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
     8941 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
     9001 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgctagag cagcttgagc
     9061 ttggatcaga ttgtcgtttc ccgccttcag tttaaactat cagtgtttga caggatatat
     9121 tggcgggtaa acctaagaga aaagagcgtt tattagaata atcggatatt taaaagggcg
     9181 tgaaaaggtt tatccgttcg tccatttgta tgtgcatgcc aaccacaggg ttcccctcgg
     9241 gatcaaagta ctttgatcca acccctccgc tgctatagtg cagtcggctt ctgacgttca
     9301 gtgcagccgt cttctgaaaa cgacatgtcg cacaagtcct aagttacgcg acaggctgcc
     9361 gccctgccct tttcctggcg ttttcttgtc gcgtgtttta gtcgcataaa gtagaatact
     9421 tgcgactaga accggagaca ttacgccatg aacaagagcg ccgccgctgg cctgctgggc
     9481 tatgcccgcg tcagcaccga cgaccaggac ttgaccaacc aacgggccga actgcacgcg
     9541 gccggctgca ccaagctgtt ttccgagaag atcaccggca ccaggcgcga ccgcccggag
     9601 ctggccagga tgcttgacca cctacgccct ggcgacgttg tgacagtgac caggctagac
     9661 cgcctggccc gcagcacccg cgacctactg gacattgccg agcgcatcca ggaggccggc
     9721 gcgggcctgc gtagcctggc agagccgtgg gccgacacca ccacgccggc cggccgcatg
     9781 gtgttgaccg tgttcgccgg cattgccgag ttcgagcgtt ccctaatcat cgaccgcacc
     9841 cggagcgggc gcgaggccgc caaggcccga ggcgtgaagt ttggcccccg ccctaccctc
     9901 accccggcac agatcgcgca cgcccgcgag ctgatcgacc aggaaggccg caccgtgaaa
     9961 gaggcggctg cactgcttgg cgtgcatcgc tcgaccctgt accgcgcact tgagcgcagc
    10021 gaggaagtga cgcccaccga ggccaggcgg cgcggtgcct tccgtgagga cgcattgacc
    10081 gaggccgacg ccctggcggc cgccgagaat gaacgccaag aggaacaagc atgaaaccgc
    10141 accaggacgg ccaggacgaa ccgtttttca ttaccgaaga gatcgaggcg gagatgatcg
    10201 cggccgggta cgtgttcgag ccgcccgcgc acgtctcaac cgtgcggctg catgaaatcc
    10261 tggccggttt gtctgatgcc aagctggcgg cctggccggc cagcttggcc gctgaagaaa
    10321 ccgagcgccg ccgtctaaaa aggtgatgtg tatttgagta aaacagcttg cgtcatgcgg
    10381 tcgctgcgta tatgatgcga tgagtaaata aacaaatacg caaggggaac gcatgaaggt
    10441 tatcgctgta cttaaccaga aaggcgggtc aggcaagacg accatcgcaa cccatctagc
    10501 ccgcgccctg caactcgccg gggccgatgt tctgttagtc gattccgatc cccagggcag
    10561 tgcccgcgat tgggcggccg tgcgggaaga tcaaccgcta accgttgtcg gcatcgaccg
    10621 cccgacgatt gaccgcgacg tgaaggccat cggccggcgc gacttcgtag tgatcgacgg
    10681 agcgccccag gcggcggact tggctgtgtc cgcgatcaag gcagccgact tcgtgctgat
    10741 tccggtgcag ccaagccctt acgacatatg ggccaccgcc gacctggtgg agctggttaa
    10801 gcagcgcatt gaggtcacgg atggaaggct acaagcggcc tttgtcgtgt cgcgggcgat
    10861 caaaggcacg cgcatcggcg gtgaggttgc cgaggcgctg gccgggtacg agctgcccat
    10921 tcttgagtcc cgtatcacgc agcgcgtgag ctacccaggc actgccgccg ccggcacaac
    10981 cgttcttgaa tcagaacccg agggcgacgc tgcccgcgag gtccaggcgc tggccgctga
    11041 aattaaatca aaactcattt gagttaatga ggtaaagaga aaatgagcaa aagcacaaac
    11101 acgctaagtg ccggccgtcc gagcgcacgc agcagcaagg ctgcaacgtt ggccagcctg
    11161 gcagacacgc cagccatgaa gcgggtcaac tttcagttgc cggcggagga tcacaccaag
    11221 ctgaagatgt acgcggtacg ccaaggcaag accattaccg agctgctatc tgaatacatc
    11281 gcgcagctac cagagtaaat gagcaaatga ataaatgagt agatgaattt tagcggctaa
    11341 aggaggcggc atggaaaatc aagaacaacc aggcaccgac gccgtggaat gccccatgtg
    11401 tggaggaacg ggcggttggc caggcgtaag cggctgggtt gcctgccggc cctgcaatgg
    11461 cactggaacc cccaagcccg aggaatcggc gtgagcggtc gcaaaccatc cggcccggta
    11521 caaatcggcg cggcgctggg tgatgacctg gtggagaagt tgaaggccgc gcaggccgcc
    11581 cagcggcaac gcatcgaggc agaagcacgc cccggtgaat cgtggcaagc ggccgctgat
    11641 cgaatccgca aagaatcccg gcaaccgccg gcagccggtg cgccgtcgat taggaagccg
    11701 cccaagggcg acgagcaacc agattttttc gttccgatgc tctatgacgt gggcacccgc
    11761 gatagtcgca gcatcatgga cgtggccgtt ttccgtctgt cgaagcgtga ccgacgagct
    11821 ggcgaggtga tccgctacga gcttccagac gggcacgtag aggtttccgc agggccggcc
    11881 ggcatggcca gtgtgtggga ttacgacctg gtactgatgg cggtttccca tctaaccgaa
    11941 tccatgaacc gataccggga agggaaggga gacaagcccg gccgcgtgtt ccgtccacac
    12001 gttgcggacg tactcaagtt ctgccggcga gccgatggcg gaaagcagaa agacgacctg
    12061 gtagaaacct gcattcggtt aaacaccacg cacgttgcca tgcagcgtac gaagaaggcc
    12121 aagaacggcc gcctggtgac ggtatccgag ggtgaagcct tgattagccg ctacaagatc
    12181 gtaaagagcg aaaccgggcg gccggagtac atcgagatcg agctagctga ttggatgtac
    12241 cgcgagatca cagaaggcaa gaacccggac gtgctgacgg ttcaccccga ttactttttg
    12301 atcgatcccg gcatcggccg ttttctctac cgcctggcac gccgcgccgc aggcaaggca
    12361 gaagccagat ggttgttcaa gacgatctac gaacgcagtg gcagcgccgg agagttcaag
    12421 aagttctgtt tcaccgtgcg caagctgatc gggtcaaatg acctgccgga gtacgatttg
    12481 aaggaggagg cggggcaggc tggcccgatc ctagtcatgc gctaccgcaa cctgatcgag
    12541 ggcgaagcat ccgccggttc ctaatgtacg gagcagatgc tagggcaaat tgccctagca
    12601 ggggaaaaag gtcgaaaagg tgtctttcct gtggatagca cgtacattgg gaacccaaag
    12661 ccgtacattg ggaaccggaa cccgtacatt gggaacccaa agccgtacat tgggaaccgg
    12721 tcacacatgt aagtgactga tataaaagag aaaaaaggcg atttttccgc ctaaaactct
    12781 ttaaaactta ttaaaactct taaaacccgc ctggcctgtg cataactgtc tggccagcgc
    12841 acagccgaag agctgcaaaa agcgcctacc cttcggtcgc tgcgctccct acgccccgcc
    12901 gcttcgcgtc ggcctatcgc ggccgctggc cgctcaaaaa tggctggcct acggccaggc
    12961 aatctaccag ggcgcggaca agccgcgccg tcgccactcg accgccggcg cccacatcaa
    13021 ggcaccctgc ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc
    13081 ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc
    13141 gtcagcgggt gttggcgggt gtcggggcgc agccatgacc cagtcacgta gcgatagcgg
    13201 agtgtatact ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg
    13261 cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct
    13321 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
    13381 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
    13441 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
    13501 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
    13561 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
    13621 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
    13681 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
    13741 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
    13801 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
    13861 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
    13921 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
    13981 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
    14041 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
    14101 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgcat
    14161 tctaggtact aaaacaattc atccagtaaa atataatatt ttattttctc ccaatcaggc
    14221 ttgatcccca gtaagtcaaa aaatagctcg acatactgtt cttccccgat atcctccctg
    14281 atcgaccgga cgcagaaggc aatgtcatac cacttgtccg ccctgccgct tctcccaaga
    14341 tcaataaagc cacttacttt gccatctttc acaaagatgt tgctgtctcc caggtcgccg
    14401 tgggaaaaga caagttcctc ttcgggcttt tccgtcttta aaaaatcata cagctcgcgc
    14461 ggatctttaa atggagtgtc ttcttcccag ttttcgcaat ccacatcggc cagatcgtta
    14521 ttcagtaagt aatccaattc ggctaagcgg ctgtctaagc tattcgtata gggacaatcc
    14581 gatatgtcga tggagtgaaa gagcctgatg cactccgcat acagctcgat aatcttttca
    14641 gggctttgtt catcttcata ctcttccgag caaaggacgc catcggcctc actcatgagc
    14701 agattgctcc agccatcatg ccgttcaaag tgcaggacct ttggaacagg cagctttcct
    14761 tccagccata gcatcatgtc cttttcccgt tccacatcat aggtggtccc tttataccgg
    14821 ctgtccgtca tttttaaata taggttttca ttttctccca ccagcttata taccttagca
    14881 ggagacattc cttccgtatc ttttacgcag cggtattttt cgatcagttt tttcaattcc
    14941 ggtgatattc tcattttagc catttattat ttccttcctc ttttctacag tatttaaaga
    15001 taccccaaga agctaattat aacaagacga actccaattc actgttcctt gcattctaaa
    15061 accttaaata ccagaaaaca gctttttcaa agttgttttc aaagttggcg tataacatag
    15121 tatcgacgga gccgattttg aaaccgcggt gatcacaggc agcaacgctc tgtcatcgtt
    15181 acaatcaaca tgctaccctc cgcgagatca tccgtgtttc aaacccggca gcttagttgc
    15241 cgttcttccg aatagcatcg gtaacatgag caaagtctgc cgccttacaa cggctctccc
    15301 gctgacgccg tcccggactg atgggctgcc tgtatcgagt ggtgattttg tgccgagctg
    15361 ccggtcgggg agctgttggc tggctggtgg caggatatat tgtggtgtaa acaaattgac
    15421 gcttagacaa cttaataaca cattgcggac gtttttaatg tactgaatta acgccgaatt
    15481 aattcgggga tagccctttg gtcttctgag actgtatctt tgatattctt ggagtagacg
    15541 agagtgtcgt gctccaccat gttggcaagc tgctctagcc aatacgcaaa ccgcctctcc
    15601 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
    15661 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
    15721 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag
    15781 gaaacagcta tgacatgatt acg
//
