LOCUS       sequence-47686-e         730 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 730)
  AUTHORS   Young CL, Raden DL, Caplan JL, Czymmek KJ, Robinson AS
  TITLE     Cassette series designed for live-cell imaging of proteins and 
            high-resolution techniques in yeast.
  JOURNAL   Yeast. 2012 Mar;29(3-4):119-36. doi: 10.1002/yea.2895. Epub 2012 Apr
            4.
  PUBMED    22473760
REFERENCE   2  (bases 1 to 730)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast."; 
            date: "2012-03"; volume: "29(3-4):119-36. doi"; pages: " 
            10.1002/yea.2895. Epub 2012 Apr 4"
COMMENT     Sequence Label: pCY 3050-07
FEATURES             Location/Qualifiers
     source          1..730
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(52..248)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     CDS             complement(254..628)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
ORIGIN
        1 atcatcgatg aattcgagct cgggatggcg gcgttagtat cgaatcgaca gcagtatagc
       61 gaccagcatt cacatacgat tgacgcatga tattactttc tgcgcactta acttcgcatc
      121 tgggcagatg atgtcgaggc gaaaaaaata taaatcacgc taacatttga ttaaaataga
      181 acaactacaa tataaaaaaa ctatacaaat gtcaagttct tgaaaacaag aatcttttta
      241 ttgtcagtac tgatcagtcc tgctcctcgg ccacgaagtg cacgcagttg ccggccgggt
      301 cgcgcagggc gaactcccgc ccccacggct gctcgccgat ctcggtcatg gccggcccgg
      361 aggcgtcccg gaagttcgtg gacacgacct ccgaccactc ggcgtacagc tcgtccaggc
      421 cgcgcaccca cacccaggcc agggtgttgt ccggcaccac ctggtcctgg accgcgctga
      481 tgaacagggt cacgtcgtcc cggaccacac cggcgaagtc gtcctccacg aagtcccggg
      541 agaacccgag ccggtcggtc cagaactcga ccgctccggc gacgtcgcgc gcggtgagca
      601 ccggaacggc gctggtcaac ttggccatgg ttgtttatgt tcggatgtga tgtgagaact
      661 gtatcctagc aagattttaa aaggaagtat atgaaagaag aacctcagtg gcaaatccta
      721 accttttata
//
