LOCUS       sequence-476885-        6021 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  5' AAVLINK plasmid for CECR2 expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6021)
  AUTHORS   Zhonghua Lu Lab
  TITLE     AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery 
            in Gene Therapy
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6021)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pAAVLINK-EF1alpha-5' CECR2
FEATURES             Location/Qualifiers
     source          1..6021
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   2284..2424
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   2284..2413
                     /label=AAV2 ITR
     rep_origin      2499..2954
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2586..2605)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2796..2817
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2971..2990)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3090..3112
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3150..3168)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3236..3340
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3341..3409,3410..4201)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3559..3578)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4372..4960
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4861..4880
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     repeat_region   5022..5151
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        5196..5407
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             5420..5688
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             5725..5751
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 tgggagctcg aagaagggaa gcccaaccct ctgagggaag ccagtttcca ggacctgcct
       61 cttcgcacac gggtggagat cctgcaccga ctctgtgatt accggctgga tgcagacgat
      121 gtcttcgatc ttctaaaggg cctggatgca gacagtctcc gtgtggagcc attgggtgaa
      181 gacaattctg gggcactata ttggtatttc tatggaacac gaatgtacaa agaggacccg
      241 gtgcaaggaa aatccaatgg agaactctct ttgagcaggg aaagtgaagg acaaaaaaat
      301 gtctcaagta ttcctggaaa aacgggaaaa agaagaggaa gacccccaaa acggaagaaa
      361 ctgcaggagg agattctgtt gagtgaaaag caggaagaaa attccttggc atccgagcca
      421 cagacaagac atgggtccca agggccaggc caaggtactt ggtggctcct gtgccagaca
      481 gaagaggaat ggagacaggt caccgagagt tttcgcgaga ggacctccct tcgagaacgg
      541 cagctctaca agctcctcag tgaggacttc ctgcctgaga tctgcaacat gatcgcccag
      601 aaggagactc ctgtgctgac cagaatagaa aaacaaaagc gcaaagagga ggaagaagag
      661 cgtcagattc ttctagcagt gcagaagaag gagcaggagc agatgctaaa ggaagagagg
      721 aaacgcgagt tggaggagaa ggtcaaggca gtggaagatc gagcgaagag gagaaagctc
      781 agggaagaaa gggcatggct gctggctcaa ggaaaggagc tccctccaga actttcccat
      841 ctggacccca attcccccat gagagaggaa aaaaagacta aagacctctt tgagttggat
      901 gatgatttca ctgctatgta taaagttcta gacgtggtaa aggctcacaa ggattcctgg
      961 cccttcttgg aacctgtgga tgaatcttat gcccctaact attatcagat tattaaggcc
     1021 cccatggata tttccagcat ggagaagaaa ctgaatggag gtttatactg taccaaggag
     1081 gaatttgtaa atgacatgaa gaccatgttc aggaattgtc gaaagtataa tggggaaagt
     1141 agtgagtata ccaagatgtc tgataattta gagaggtgtt tccatcgggc aatgatgaaa
     1201 cattttcctg gagaagatgg agacacagat gaagaatttt ggattcgaga ggatgaaaag
     1261 cgggagaaaa gacggagtcg ggctgggcga agtggtggga gccatgtttg gacccgctcc
     1321 agggacccag aagggtccag caggaaacag cagcccatgg agaatggagg aaagtcgttg
     1381 ccccccacac gccgagcgcc ctcttctggg gacgatcaga gcagcagctc cacacagccc
     1441 ccgcgggagg tgggcacttc caatggccga ggtttttctc atcccctgca ttgtggtggg
     1501 acacccagcc aggcaccctt tttaaaccag atgaggccag cagtaccagg aacatttggc
     1561 cctctgcgag gatcagatcc tgccaccttg tatggctcct ctggagtccc ggagccacac
     1621 cacggggagc ctgtgcagca gcgtcagcct ttcaccatgc agcctccagt tggaattaac
     1681 agcctccgag gacccaggct aggcacacca gaggagaagc aaatgtgcgg ggggctgaca
     1741 cacctttcta acatgggccc acaccctgga tccttgcagc ttgggcagat aagtggccca
     1801 agtcaggatg gaagcatgta tgctccagct cagttccagc caggattcat tcctccccgg
     1861 catggggggg ctccagcccg gccaccagac tttcctgaaa gctcagaaat tcctcccagc
     1921 catatgtatc gatcgtacaa gtacctgaat cgagtacact ctgccgtctg gaatgggaac
     1981 catggtgcta cgaaccaagg acccttgggc ccagatgaga agccccacct ggggccagga
     2041 ccctctcacc agcctcgcac tctcggtcac gtgatggatt cccgagtcat gagaccacct
     2101 gtccccccca accagtggac tgaacaatca ggcttcctac ctcatggagt tccttcctca
     2161 ggtaagtatc aaggttacaa gacaggttta aggagaccaa taagctatta ttcgtatagg
     2221 atactttata cgaagttatt aagttatgga gaccggtgtg gcacgtgcgg accgagcggc
     2281 cgcaggaacc cctagtgatg gagttggcca ctccctctct gcgcgctcgc tcgctcactg
     2341 aggccgggcg accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg
     2401 agcgagcgcg cagctgcctg caggggcgcc tgatgcggta ttttctcctt acgcatctgt
     2461 gcggtatttc acaccgcata cgtcaaagca accatagtac gcgccctgta gcggcgcatt
     2521 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccttagc
     2581 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     2641 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     2701 caaaaaactt gatttgggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     2761 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     2821 aacactcaac tctatctcgg gctattcttt tgatttataa gggattttgc cgatttcggt
     2881 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt
     2941 aacgtttaca attttatggt gcactctcag tacaatctgc tctgatgccg catagttaag
     3001 ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc
     3061 atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc
     3121 gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa
     3181 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
     3241 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     3301 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     3361 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     3421 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     3481 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     3541 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     3601 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     3661 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     3721 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     3781 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     3841 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     3901 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     3961 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     4021 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     4081 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     4141 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     4201 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     4261 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     4321 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     4381 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     4441 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     4501 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     4561 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     4621 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     4681 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     4741 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     4801 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     4861 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     4921 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     4981 tttacggttc ctggcctttt gctggccttt tgctcacatg tcctgcaggc agctgcgcgc
     5041 tcgctcgctc actgaggccg cccgggcgtc gggcgacctt tggtcgcccg gcctcagtga
     5101 gcgagcgagc gcgcagagag ggagtggcca actccatcac taggggttcc tgcggccgca
     5161 cgcgtggatc tgcgatcgct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt
     5221 ccccgagaag ttggggggag gggtcggcaa ttgaacgggt gcctagagaa ggtggcgcgg
     5281 ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga
     5341 accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag
     5401 aacacagctg aagcttcgag gggctcgcat ctctccttca cgcgcccgcc gccctacctg
     5461 aggccgccat ccacgccggt tgagtcgcgt tctgccgcct cccgcctgtg gtgcctcctg
     5521 aactgcgtcc gccgtctagg taagtttaaa gctcaggtcg agaccgggcc tttgtccggc
     5581 gctcccttgg agcctaccta gactcagccg gctctccacg ctttgcctga ccctgcttgc
     5641 tcaactctac gtctttgttt cgttttctgt tctgcgccgt tacagatcca agctgtgacc
     5701 ggcgcctacc gtacggccac catgtaccct tatgacgtac ctgactatgc tggatcttgc
     5761 ccagaggagg gcggcgcggc cgggctgggc gagctccgct cctggtggga ggtcccggcc
     5821 atcgcgcact tctgctcgct ctttcgcacc gcgttccgcc tgcccgactt cgagatcgag
     5881 gagttagaag ccgctcttca cagagatgac gtggagttta tcagtgacct gattgcctgc
     5941 ctgcttcagg gctgctatca acgaagagat atcacgcctc agacattcca cagctaccta
     6001 gaggacatca tcaactaccg c
//
