LOCUS       sequence-476894-       19822 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  All-in-one plant virus expression vector for virus-induced gene 
            silencing (VIGS)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 19822)
  AUTHORS   Hao Y, Yuan Z, Li Y, Zuo D, Cheng H, Wang Q, Zhang Y, Lv L, Liu J, 
            Song G
  TITLE     An all-in-one plant virus-based vector toolkit for streamlined gene 
            silencing, overexpression, and genome editing.
  JOURNAL   Plant Commun. 2025 May 19:101379. doi: 10.1016/j.xplc.2025.101379.
  PUBMED    40394900
REFERENCE   2  (bases 1 to 19822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Commun. 2025 May 19:101379. doi: 10.1016/j.xplc.2025.101379."
COMMENT     Sequence Label: pVS2e
FEATURES             Location/Qualifiers
     source          1..19822
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             687..704
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      739..991
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(1000..1016)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1000..1016)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     misc_feature    1037..1061
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             1526..2176
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     primer_bind     complement(2364..2381)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(2615..2634)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     oriT            2857..2966
                     /note="incP origin of transfer"
     CDS             2999..3370
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     rep_origin      3497..4208
                     /label=oriV
                     /note="incP origin of replication"
     mobile_element  4831..5598
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     CDS             5790..6584
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     CDS             6883..8031
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKTYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    9627..9651
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     primer_bind     complement(9898..9919)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     10110..10129
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10254..10276
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     10268..10285
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     10269..10285
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(10294..10546)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        complement(17400..17745)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     promoter        18275..18321
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
ORIGIN
        1 acgctgtttg agggaaaagt agagaacgta tttgttttta tgttcaggcg gttcttgtgt
       61 gtcaacaaag atggacattg ttactcaagg aagcacgatg agctttatta ttacggacga
      121 gtggacttag attctgtgag taaggttacc gaattggcca gttaacgcta gcgaatcgag
      181 acctcttaca cggtctcact agtctgcagg catgccattc gaggcccggg catgtcccga
      241 agacattaaa ctacggttct ttaagtagat ccgtgtctga agttttaggt tcaatttaaa
      301 cctacgagat tgacattctc gactgatctt gattgatcgg taagtctttt gtaatttaat
      361 tttctttttg attttatttt aaattgttat ctgtttctgt gtatagactg tttgagatcg
      421 gcgtttggcc gactcattgt cttaccatag gggaacggac tttgtttgtg ttgttatttt
      481 atttgtattt tattaaaatt ctcaacgatc tgaaaaagcc tcgcggctaa gagattgttg
      541 gggggtgagt aagtactttt aaagtgatga tggttacaaa ggcaaaaggg gtaaaacccc
      601 tcgcctacgt aagcgttatt acgcccgtct gtacttatat cagtacactg acgagtccct
      661 aaaggacgaa acgggagaac gctagccacc accaccacca ccacgtgtga attacaggtg
      721 accagctcga atttccccga tcgttcaaac atttggcaat aaagtttctt aagattgaat
      781 cctgttgccg gtcttgcgat gattatcata taatttctgt tgaattacgt taagcatgta
      841 ataattaaca tgtaatgcat gacgttattt atgagatggg tttttatgat tagagtcccg
      901 caattataca tttaatacgc gatagaaaac aaaatatagc gcgcaaacta ggataaatta
      961 tcgcgcgcgg tgtcatctat gttactagat cgggaattgg tcatagctgt ttcctgggat
     1021 ccaaactatc agtgtttgac aggatatatt ggcgggtaaa cctaagagaa aagagcgttt
     1081 attagaataa tcggatattt aaaagggcgt gaaaaggttt atccgttcgt ccatttgtat
     1141 gtgcatgcca accacagggt tccccagatc tggcgccggc cagcgagacg agcaagattg
     1201 gccgccgccc gaaacgatcc gacagcgcgc ccagcacagg tgcgcaggca aattgcacca
     1261 acgcatacag cgccagcaga atgccatagt gggcggtgac gtcgttcgag tgaaccagat
     1321 cgcgcaggag gcccggcagc accggcataa tcaggccgat gccgacagcg tcgagcgcga
     1381 cagtgctcag aattacgatc aggggtatgt tgggtttcac gtctggcctc cggaccagcc
     1441 tccgctggtc cgattgaacg cgcggattct ttatcactga taagttggtg gacatattat
     1501 gtttatcagt gataaagtgt caagcatgac aaagttgcag ccgaatacag tgatccgtgc
     1561 cgccctggac ctgttgaacg aggtcggcgt agacggtctg acgacacgca aactggcgga
     1621 acggttgggg gttcagcagc cggcgcttta ctggcacttc aggaacaagc gggcgctgct
     1681 cgacgcactg gccgaagcca tgctggcgga gaatcatacg cattcggtgc cgagagccga
     1741 cgacgactgg cgctcatttc tgatcgggaa tgcccgcagc ttcaggcagg cgctgctcgc
     1801 ctaccgcgat ggcgcgcgca tccatgccgg cacgcgaccg ggcgcaccgc agatggaaac
     1861 ggccgacgcg cagcttcgct tcctctgcga ggcgggtttt tcggccgggg acgccgtcaa
     1921 tgcgctgatg acaatcagct acttcactgt tggggccgtg cttgaggagc aggccggcga
     1981 cagcgatgcc ggcgagcgcg gcggcaccgt tgaacaggct ccgctctcgc cgctgttgcg
     2041 ggccgcgata gacgccttcg acgaagccgg tccggacgca gcgttcgagc agggactcgc
     2101 ggtgattgtc gatggattgg cgaaaaggag gctcgttgtc aggaacgttg aaggaccgag
     2161 aaagggtgac gattgatcag gaccgctgcc ggagcgcaac ccactcacta cagcagagcc
     2221 atgtagacaa catcccctcc ccctttccac cgcgtcagac gcccgtagca gcccgctacg
     2281 ggctttttca tgccctgccc tagcgtccaa gcctcacggc cgcgctcggc ctctctggcg
     2341 gccttctggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     2401 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     2461 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     2521 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     2581 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     2641 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     2701 ctcccttcgg gaagcgtggc gcttttccgc tgcataaccc tgcttcgggg tcattatagc
     2761 gattttttcg gtatatccat cctttttcgc acgatataca ggattttgcc aaagggttcg
     2821 tgtagacttt ccttggtgta tccaacggcg tcagccgggc aggataggtg aagtaggccc
     2881 acccgcgagc gggtgttcct tcttcactgt cccttattcg cacctggcgg tgctcaacgg
     2941 gaatcctgct ctgcgaggct ggccggctac cgccggcgta acagatgagg gcaagcggat
     3001 ggctgatgaa accaagccaa ccaggaaggg cagcccacct atcaaggtgt actgccttcc
     3061 agacgaacga agagcgattg aggaaaaggc ggcggcggcc ggcatgagcc tgtcggccta
     3121 cctgctggcc gtcggccagg gctacaaaat cacgggcgtc gtggactatg agcacgtccg
     3181 cgagctggcc cgcatcaatg gcgacctggg ccgcctgggc ggcctgctga aactctggct
     3241 caccgacgac ccgcgcacgg cgcggttcgg tgatgccacg atcctcgccc tgctggcgaa
     3301 gatcgaagag aagcaggacg agcttggcaa ggtcatgatg ggcgtggtcc gcccgagggc
     3361 agagccatga cttttttagc cgctaaaacg gccggggggt gcgcgtgatt gccaagcacg
     3421 tccccatgcg ctccatcaag aagagcgact tcgcggagct ggtgaagtac atcaccgacg
     3481 agcaaggcaa gaccgagcgc ctttccgacg ctcaccgggc tggttgccct cgccgctggg
     3541 ctggcggccg tctatggccc tgcaaacgcg ccagaaacgc cgtcgaagcc gtgtgcgaga
     3601 caccgcggcc gccggcgttg tggatacctc gcggaaaact tggccctcac tgacagatga
     3661 ggggcggacg ttgacacttg aggggccgac tcacccggcg cggcgttgac agatgagggg
     3721 caggctcgat ttcggccggc gacgtggagc tggccagcct cgcaaatcgg cgaaaacgcc
     3781 tgattttacg cgagtttccc acagatgatg tggacaagcc tggggataag tgccctgcgg
     3841 tattgacact tgaggggcgc gactactgac agatgagggg cgcgatcctt gacacttgag
     3901 gggcagagtg ctgacagatg aggggcgcac ctattgacat ttgaggggct gtccacaggc
     3961 agaaaatcca gcatttgcaa gggtttccgc ccgtttttcg gccaccgcta acctgtcttt
     4021 taacctgctt ttaaaccaat atttataaac cttgttttta accagggctg cgccctgtgc
     4081 gcgtgaccgc gcacgccgaa ggggggtgcc cccccttctc gaaccctccc ggcccgctaa
     4141 cgcgggcctc ccatcccccc aggggctgcg cccctcggcc gcgaacggcc tcaccccaaa
     4201 aatggcagcg ctggcagtcc ttgccattgc cgggatcggg gcagtaacgg gatgggcgat
     4261 cagcccgagc gcgacgcccg gaagcattga cgtgccgcag gtgctggcat cgacattcag
     4321 cgaccaggtg ccgggcagtg agggcggcgg cctgggtggc ggcctgccct tcacttcggc
     4381 cgtcggggca ttcacggact tcatggcggg gccggcaatt tttaccttgg gcattcttgg
     4441 catagtggtc gcgggtgccg tgctcgtgtt cgggggtgcg ataaacccag cgaaccattt
     4501 gaggtgatag gtaagattat accgaggtat gaaaacgaga attggacctt tacagaatta
     4561 ctctatgaag cgccatattt aaaaagctac caagacgaag aggatgaaga ggatgaggag
     4621 gcagattgcc ttgaatatat tgacaatact gataagataa tatatctttt atatagaaga
     4681 tatcgccgta tgtaaggatt tcagggggca aggcataggc agcgcgctta tcaatatatc
     4741 tatagaatgg gcaaagcata aaaacttgca tggactaatg cttgaaaccc aggacaataa
     4801 ccttatagct tgtaaattct atcataattg ggtaatgact ccaacttatt gatagtgttt
     4861 tatgttcaga taatgcccga tgactttgtc atgcagctcc accgattttg agaacgacag
     4921 cgacttccgt cccagccgtg ccaggtgctg cctcagattc aggttatgcc gctcaattcg
     4981 ctgcgtatat cgcttgctga ttacgtgcag ctttcccttc aggcgggatt catacagcgg
     5041 ccagccatcc gtcatccata tcaccacgtc aaagggtgac agcaggctca taagacgccc
     5101 cagcgtcgcc atagtgcgtt caccgaatac gtgcgcaaca accgtcttcc ggagactgtc
     5161 atacgcgtaa aacagccagc gctggcgcga tttagccccg acatagcccc actgttcgtc
     5221 catttccgcg cagacgatga cgtcactgcc cggctgtatg cgcgaggtta ccgactgcgg
     5281 cctgagtttt ttaagtgacg taaaatcgtg ttgaggccaa cgcccataat gcgggctgtt
     5341 gcccggcatc caacgccatt catggccata tcaatgattt tctggtgcgt accgggttga
     5401 gaagcggtgt aagtgaactg cagttgccat gttttacggc agtgagagca gagatagcgc
     5461 tgatgtccgg cggtgctttt gccgttacgc accaccccgt cagtagctga acaggaggga
     5521 cagctgatag aaacagaagc cactggagca cctcaaaaac accatcatac actaaatcag
     5581 taagttggca gcatcaccca taattgtggt ttcaaaatcg gctccgtcga tactatgtta
     5641 tacgccaact ttgaaaacaa ctttgaaaaa gctgttttct ggtatttaag gttttagaat
     5701 gcaaggaaca gtgaattgga gttcgtcttg ttataattag cttcttgggg tatctttaaa
     5761 tactgtagaa aagaggaagg aaataataaa tggctaaaat gagaatatca ccggaattga
     5821 aaaaactgat cgaaaaatac cgctgcgtaa aagatacgga aggaatgtct cctgctaagg
     5881 tatataagct ggtgggagaa aatgaaaacc tatatttaaa aatgacggac agccggtata
     5941 aagggaccac ctatgatgtg gaacgggaaa aggacatgat gctatggctg gaaggaaagc
     6001 tgcctgttcc aaaggtcctg cactttgaac ggcatgatgg ctggagcaat ctgctcatga
     6061 gtgaggccga tggcgtcctt tgctcggaag agtatgaaga tgaacaaagc cctgaaaaga
     6121 ttatcgagct gtatgcggag tgcatcaggc tctttcactc catcgacata tcggattgtc
     6181 cctatacgaa tagcttagac agccgcttag ccgaattgga ttacttactg aataacgatc
     6241 tggccgatgt ggattgcgaa aactgggaag aagacactcc atttaaagat ccgcgcgagc
     6301 tgtatgattt tttaaagacg gaaaagcccg aagaggaact tgtcttttcc cacggcgacc
     6361 tgggagacag caacatcttt gtgaaagatg gcaaagtaag tggctttatt gatcttggga
     6421 gaagcggcag ggcggacaag tggtatgaca ttgccttctg cgtccggtcg atcagggagg
     6481 atatcgggga agaacagtat gtcgagctat tttttgactt actggggatc aagcctgatt
     6541 gggagaaaat aaaatattat attttactgg atgaattgtt ttagtaccta gatgtggcgc
     6601 aacgatgccg gcgacaagca ggagcgcacc gacttcttcc gcatcaagtg ttttggctct
     6661 caggccgagg cccacggcaa gtatttgggc aaggggtcgc tggtattcgt gcagggcaag
     6721 attcggaata ccaagtacga gaaggacggc cagacggtct acgggaccga cttcattgcc
     6781 gataaggtgg attatctgga caccaaggca ccaggcgggt caaatcagga ataagggcac
     6841 attgccccgg cgtgagtcgg ggcaatcccg caaggagggt gaatgaatcg gacgtttgac
     6901 cggaagacat acaggcaaga actgatcgac gcggggtttt ccgccgagga tgccgaaacc
     6961 atcgcaagcc gcaccgtcat gcgtgcgccc cgcgaaacct tccagtccgt cggctcgatg
     7021 gtccagcaag ctacggccaa gatcgagcgc gacagcgtgc aactggctcc ccctgccctg
     7081 cccgcgccat cggccgccgt ggagcgttcg cgtcgtctcg aacaggaggc ggcaggtttg
     7141 gcgaagtcga tgaccatcga cacgcgagga actatgacga ccaagaagcg aaaaaccgcc
     7201 ggcgaggacc tggcaaaaca ggtcagcgag gccaagcagg ccgcgttgct gaaacacacg
     7261 aagcagcaga tcaaggaaat gcagctttcc ttgttcgata ttgcgccgtg gccggacacg
     7321 atgcgagcga tgccaaacga cacggcccgc tctgccctgt tcaccacgcg caacaagaaa
     7381 atcccgcgcg aggcgctgca aaacaaggtc attttccacg tcaacaagga cgtgaagatc
     7441 acctacaccg gcgtcgagct gcgggccgac gatgacgaac tggtgtggca gcaggtgttg
     7501 gagtacgcga agcgcacccc tatcggcgag ccgatcacct tcacgttcta cgagctttgc
     7561 caggacctgg gctggtcgat caatggccgg tattacacga aggccgagga atgcctgtcg
     7621 cgcctacagg cgacggcgat gggcttcacg tccgaccgcg ttgggcacct ggaatcggtg
     7681 tcgctgctgc accgcttccg cgtcctggac cgtggcaaga aaacgtcccg ttgccaggtc
     7741 ctgatcgacg aggaaatcgt cgtgctgttt gctggcgacc actacacgaa attcatatgg
     7801 gagaagtacc gcaagctgtc gccgacggcc cgacggatgt tcgactattt cagctcgcac
     7861 cgggagccgt acccgctcaa gctggaaacc ttccgcctca tgtgcggatc ggattccacc
     7921 cgcgtgaaga agtggcgcga gcaggtcggc gaagcctgcg aagagttgcg aggcagcggc
     7981 ctggtggaac acgcctgggt caatgatgac ctggtgcatt gcaaacgcta gggccttgtg
     8041 gggtcagttc cggctggggg ttcagcagcc agcgctttac tggcatttca ggaacaagcg
     8101 ggcactgctc gacgcacttg cttcgctcag tatcgctcgg gacgcacggc gcgctctacg
     8161 aactgccgat aaacagagga ttaaaattga caattgtgat taaggctcag attcgacggc
     8221 ttggagcggc cgacgtgcag gatttccgcg agatccgatt gtcggccctg aagaaagctc
     8281 cagagatgtt cgggtccgtt tacgagcacg aggagaaaaa gcccatggag gcgttcgctg
     8341 aacggttgcg agatgccgtg gcattcggcg cctacatcga cggcgagatc attgggctgt
     8401 cggtcttcaa acaggaggac ggccccaagg acgctcacaa ggcgcatctg tccggcgttt
     8461 tcgtggagcc cgaacagcga ggccgagggg tcgccggtat gctgctgcgg gcgttgccgg
     8521 cgggtttatt gctcgtgatg atcgtccgac agattccaac gggaatctgg tggatgcgca
     8581 tcttcatcct cggcgcactt aatatttcgc tattctggag cttgttgttt atttcggtct
     8641 accgcctgcc gggcggggtc gcggcgacgg taggcgctgt gcagccgctg atggtcgtgt
     8701 tcatctctgc cgctctgcta ggtagcccga tacgattgat ggcggtcctg ggggctattt
     8761 gcggaactgc gggcgtggcg ctgttggtgt tgacaccaaa cgcagcgcta gatcctgtcg
     8821 gcgtcgcagc gggcctggcg ggggcggttt ccatggcgtt cggaaccgtg ctgacccgca
     8881 agtggcaacc tcccgtgcct ctgctcacct ttaccgcctg gcaactggcg gccggaggac
     8941 ttctgctcgt tccagtagct ttagtgtttg atccgccaat cccgatgcct acaggaacca
     9001 atgttctcgg cctggcgtgg ctcggcctga tcggagcggg tttaacctac ttcctttggt
     9061 tccgggggat ctcgcgactc gaacctacag ttgtttcctt actgggcttt ctcagcccca
     9121 gatctggggt cgatcagccg gggatgcatc aggccgacag tcggaacttc gggtccccga
     9181 cctgtaccat tcggtgagca atggataggg gagttgatat cgtcaacgtt cacttctaaa
     9241 gaaatagcgc cactcagctt cctcagcggc tttatccagc gatttcctat tatgtcggca
     9301 tagttctcaa gatcgacagc ctgtcacggt taagcgagaa atgaataaga aggctgataa
     9361 ttcggatctc tgcgagggag atgatatttg atcacaggca gcaacgctct gtcatcgtta
     9421 caatcaacat gctaccctcc gcgagatcat ccgtgtttca aacccggcag cttagttgcc
     9481 gttcttccga atagcatcgg taacatgagc aaagtctgcc gccttacaac ggctctcccg
     9541 ctgacgccgt cccggactga tgggctgcct gtatcgagtg gtgattttgt gccgagctgc
     9601 cggtcgggga gctgttggct ggctggtggc aggatatatt gtggtgtaaa caaattgacg
     9661 cttagacaac ttaataacac attgcggacg tttttaatgt actggggtgg tttttctttt
     9721 caccagtgag acgggcaaca gctgattgcc cttcaccgcc tggccctgag agagttgcag
     9781 caagcggtcc acgctggttt gccccagcag gcgaaaatcc tgtttgatgg tggttccgaa
     9841 atcggcaaaa tcccttataa atcaaaagaa tagcccgaga tagggttgag tgttgttcca
     9901 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc
     9961 gtctatcagg gcgatggccc actacgtgaa ccatcaccca aatcaagttt tttggggtcg
    10021 aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg
    10081 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgccatt
    10141 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct
    10201 ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc
    10261 acgacgttgt aaaacgacgg ccagtgaatt cccgatctag taacatagat gacaccgcgc
    10321 gcgataattt atcctagttt gcgcgctata ttttgttttc tatcgcgtat taaatgtata
    10381 attgcgggac tctaatcata aaaacccatc tcataaataa cgtcatgcat tacatgttaa
    10441 ttattacatg cttaacgtaa ttcaacagaa attatatgat aatcatcgca agaccggcaa
    10501 caggattcaa tcttaagaaa ctttattgcc aaatgtttga acgatcgggg aaattcgagg
    10561 ggcccgtttc gtcctttagg gactcgtcag tgtactgata taagtacaga cgggcgtaat
    10621 aacgcttacg taggcgaggg gttttacccc ttttgccttt gtaaccatca tcactttaaa
    10681 agtacttact caccccccaa taatctctta gcctcgaggc cttttcagat cattgagaat
    10741 tttaataaaa tacaaataaa ataacaacac aaacaaagtc cgttccccta tggtaagaca
    10801 atgagtcggc caagcgccaa tctcaaacag tctatataca gaaacagata acaatatata
    10861 ataaaatcaa aaagcaaaca aacgatcaat cccgaaagga acatcatctc ttaaaaatct
    10921 ttttggtgtc ccaaattctc tgtcctcttt gaatttcttc ttagaggtct tcgaattatg
    10981 agacttttta gctgtcgcag acatgttttc agcttgattt cgtcgaattt gtttccaaat
    11041 ttctcgattt cttgtttcaa cacgtttacg acacttttca aggtgactac ggccacaaca
    11101 attatacaca tcaaaagtaa aatcattgat aacaacacag acaaaccatc cacaattatt
    11161 ttccgcacac ctacgtgtga caccaaccat gtcagcaact tgctttcgca atttgttagc
    11221 atgaccgata ctacacgtct cgtgaccaag aacagtgact tcattcacac aacccttgag
    11281 tacacacgtc atattgacaa taaatttctt tatgcattaa gacgagtttt tcttattaga
    11341 cgctctctta acatcttcac ttgacaataa tgacttaggt ctagtagtag ctggtctgta
    11401 aggaccatcg tacttcgcct caatcgtctt catctccact tgtttcaaag acatgaactc
    11461 accgaaaacc tcttgatcgg taaaatcgcc ttcaatgtaa tccgaactgt tcagttcttc
    11521 cacattgagg cgcctagtcg aatcagtagc aacccatatt agcgacactt cgatactgaa
    11581 agggtatgta ccgtcacaga ccggaacgcc ctcagaaata caacccacct tccagacatc
    11641 aagtacgttc tcctgtgtgg aaatactgta ttcaaccttg tactgcgcaa ctgaacagtt
    11701 atcgcaagct cgagctttga atctctgaat agtctttcct ctctttcttt tctcagtaag
    11761 tctcgaatcc aacagatcga tttgaatgaa cccaggcgta tctgcaggta gatgtgatgt
    11821 tacaaccaat tgtacaccta ctatctttag aaacgcgtaa gatttaatca ttttgtaatc
    11881 cctttggtca aaaacgtcta acttgtgttc actcttcttc ccaccaacat gatttaggac
    11941 agtttctctt cttctgtgaa aatacttcgg cctcttcctc ctaccgtcca caaacaattc
    12001 aatggcccct agatttgaga attttgagac ttcgaaagtc ttcttcttca aggtgaccaa
    12061 tgacttgtct tccatattac cagtcgtaaa agtttgacac agctctctgt gccttcttcc
    12121 aatcaacctt agccgggttc aattccttat cgttttcgtc tcggaataat gtacacagag
    12181 ctgtaaaact cttaatatgc ttccatagcg cacaaagcgc atgaacagaa tcacctttgt
    12241 acaaataccg gtctgaaaca gactcggaca gtttggatac caccatgtag ttcccaagag
    12301 ctctattgga atcattcagc gagatgtaga tctcggctaa atgttgcaca tcctttatac
    12361 tctttttccc caacttcgtc agaactttta ccggatctgg cacgaactcg taacacgatg
    12421 acgtcttaag caagaacttc ccacaaaaca ttgggacatc gtacttaaaa atcttgcact
    12481 cgaaattcca cttagtagcc aactttggac acggatcaac aaactgcgtt cctctaggaa
    12541 acgcaatcag tgagtcatct cctccatatg taaccatgac tgctttctcc aatggtaatt
    12601 cagacaagag tgcacacagt gttctatctg aatttgcatt ataagtatca gcatctccac
    12661 tcttttgttg gtaccaaata tgcgccatca taccattttg aatatctctc acagtagttt
    12721 gagtgtgcga cacctcccaa aggaaggccg cccactcatc tagcccaaat aacctgtaaa
    12781 tctccagctg cagttgtaaa tggaagcgat ttgccgactt gtcgaattta gacatgtcta
    12841 tctcaacaaa gtcgtaagcc gcttccgtat ttaagaactt cactcgatcg tttaaatcac
    12901 tcgatgtcat tctcgtgttg aacacaaaat gtggttgcat agcatccaac ttgcgttcat
    12961 taatttcttt gaaaattgga ccgaacaacg agttgatcaa cttctcgtga tacacaacag
    13021 tctgtagagc tgagtattca aattgcggtg ttaaatcagt cttaggtttc acgtcagatt
    13081 tgatcatata catgtaagac gaatagtcta tttcatgtaa ctctctcaca tctgctacta
    13141 ccttagcctg taccgctgtg ctttgatttc tccaccatct ctccatttga gctctattga
    13201 caataggatc agcccgaatt tttcccacat cgtagacaac agatttcagc ttcttcatcg
    13261 tctcttcgat tagaactgtc gcgtgcacat tttcttgtag atcgggtgcc gcttggttcc
    13321 ttttctgcaa tgctaacaaa ctctccaact gagtagtctt ccttttgtca ggcatagcag
    13381 tacgaataac cggtttcaga aacgtttctt tttcagcaaa cttgtctttc cagtttccac
    13441 ttttgatcgt aacattgtct aatcgcaaat tgcaatcagt cgttgccacc aaatacccgt
    13501 ctaggcttga gtctcttaac gaatttcccg gaaacaaagc gtcgtaccac atctccaagt
    13561 ccgtaatcgt acctgaatct ggaacggcgc aaggcccttc atgatgtcta aagacatcaa
    13621 acctagaccg aaaccgtcat aagaccgtct cagtaacaaa aaatcttgcc aaagccgcct
    13681 tcgtaagcgc ggcactttcc ctgatctctt tgcttacatc gtcctctttc acagtttcat
    13741 acacaagtga ttgtgtgtga cgcgacaacg ccacgattaa gtactcccga cctctagcga
    13801 ttgagtcatc cgtaggtttc gtcctgacta ggactacatc tttgaatgtc tctccttgcg
    13861 actcgtgtac tgtactcact ttagctgcca ctttcctcaa tgccaacaaa tttaccaact
    13921 ccttcttctc agactgaagg aaagttatgt actgagcacc aggcttcaaa gaaacctgtt
    13981 ctttagacac aatcttcctc accgtcatcg aattagcagt cgcgttatgt gttctgacat
    14041 ctccagtata gtacttgttc aatacggcag ccacatctgc tggacttctg taagtttctc
    14101 ttttttctgt aacaatgtca aactttccga ccagactaga aaacctcaaa tcaacttgag
    14161 ataccctagg cttgaaagaa atttgattct gatctccttg acagatacat cgtttagcac
    14221 cagctatctg agcgcaaaag tacaccatac cagcatgggc catgagagct tcatcgaaat
    14281 gcaacacgtc tccggttaaa gaaccatcaa cacaatgcat caaaaaagaa tcaaccgtct
    14341 tcactctcct tttcaatttg catggaaaac ctttgctcgc gaatctctcg atcaagtcgt
    14401 cggttgctgc tctcccagta gagagaacca catcgacaca aggattagct gagttgacaa
    14461 tcattgtcga cttaccacaa ccaggtacac cgtcaactaa ttcaaaagaa cagttaactg
    14521 gtcctagatc cagggcactt aaattgtcca agatcaacct gttattgcaa agatacgtct
    14581 gatcgcaaac tatgacataa tcactcatcc tttcgaccgc gaagtcgttg accaacatac
    14641 ctcgttccca aacagccact tctaagtatc ccgaccaatc atccatacgt ttgtctggtt
    14701 ggaacacata agcatgttca gtgaccactg gacgtatgca ccagttcttc atctccacat
    14761 cccacactcc cagtccgtgc gggtaattct gacctccagt tcgaataact cctctcgaat
    14821 agtcacgata cagagacata atttcatcat tttgagcgac tctctccgca tctaagtagt
    14881 acagaaactc tcttctagcg tccacagaat cgtctccact gacaaccggc ctttttggta
    14941 atctctcacc tcccatcact tggaagtaat cgacacgttt gaccgttttt cctttgctga
    15001 ccaaaggttt gacagctggc aacacgttct caaatcctgg tggcgttaaa ggtttacaca
    15061 acacctttgt accgcttgtt tcccctcttt cacattcagc tgaaaaggct gaaacaacct
    15121 cgtcaatttc tgcttcagtg tcaataagac cactcagaaa ctgctccatg atctcctcat
    15181 cgtctacctt tcttgaaact ccaactctag cttgggtcag cacgttactg tcttccaaca
    15241 acagacccag tccgcttcga gatgaaccga ccaccgatgg tggagaagtt ctcaccgaac
    15301 tgccagaata gttagagaat tttgcactta gaaactcgtc agcgatttct ttcttctgct
    15361 gcaatagctt atcagacttg gcttttacct cggataaatc tggtaaaacc gtctcaccgg
    15421 actcagatgc cgaatacaga ctgctaaaga aatcgttgat ttcataatag cggcacggat
    15481 tctcaacgtc taagacatgg gccccccgtt tgatatcaaa catatcacat agttttttca
    15541 ccagtccatc tcggatgtct cgacgcataa gcgtgattcc tccaatggca tacacaacac
    15601 tcagaaatac tatcaccttg gttttgtgcg agttctcgta acatagttct ggaccatttg
    15661 gtgtgtcctt cgcccaggtt agtaccttct ttggatagaa tccaattaga gtaccaacca
    15721 tgccaacgaa tccgccgact gcgatcttgt tagcaaactg cttccacagt tttcttgata
    15781 ttaaacccag cacatcagtc aatgacgtga taggtttcgt ctcagtcaga attgcttcac
    15841 gcaactccct catgacaggt cgcgccactt gttccttcac tagcaaagtt tgagccaaca
    15901 actgtaaatc tcgagaatct acactttgct tgttcttcac ggccgccccg tttatgaata
    15961 agacccaatt atttgaactc aagtatgatt taacattgct tatggtcagc tgctggtcag
    16021 ataacctagc tatgtaatcc aaacacttgt ccatgaattg tttctctaca aacaggtctt
    16081 tcttgaccaa ctctcgcgtt gattcgacca gatcgaaaat tgggacaaca accatgtttt
    16141 cccatctact gatatatatt cttcggtagt actcttgcga tgatagcgac ctcctcggaa
    16201 ctccagctat cctgtagatc gaaaaaagca ttgtgtctcc tctcacctgc cacggttcga
    16261 agtagaacac attcccttgg taactcactg gcttccccag tagatacttc ttgtactctt
    16321 cccagtcatg aatgtaagaa aaggaaggat ctttctcaaa accgaaatag atcttcccgt
    16381 tcttcttcat gtagtaaccg tcaacagaag gtaatggacc ttcgtccact aacatacttt
    16441 caggagcaaa aagcatagca gcatgaacca ctttagtttt cttctcaacc atagtagcca
    16501 tcaagtcttt caacttgaag tcatacaggc tgtgaagagc tatcgcatac tgcgcgcctt
    16561 tcttcttacc atcagaaaaa cccctacatg tacaaccctg atatgtatta ttgcacctaa
    16621 ccgcgttagg cgcagcaata ttttcacgaa attggtcata catccacagc tcattctccg
    16681 acatctgtct cttaccactt ctagcttgat caccaatagc agtaagaaat atctgtcgag
    16741 aaatttctct ctgatcatcc cgaatatcta gatttggaca acaggaatga atccttttct
    16801 cacctctagt tacccaaaac gaccattgac caccgatgtc atccacctct tgcagagcag
    16861 gaactctatc taaaagcaat aacgcttgta gttttcgctc agcagccgca aacccatgaa
    16921 ccatgttttt gtcgtcttta aacacgatat tgaattcagg ataaatttcc atcaacttgt
    16981 tctgttcctt ctgagaaaga acttcatgaa caatcactgg atctttagtc tttctttctc
    17041 taatcatgtc atcgacttta tcaacaacaa ctctttgcat catttgcccg atctcacaat
    17101 caggtttagt cagcatcaag tcaaagaaat gactcctctg ctcagcagac atctcgtcca
    17161 cattgagcaa ttgagacaac ttgaagttac cgttcgccat tgtatctgta tgtattttta
    17221 caaagtatca aagaaaaggt tttaggcaca ccagtcactt tcccccactc ggtactaagc
    17281 tggatagatt aaaccaaaaa gaaaaacaaa ataaaactgt aggtgataag tacgttctac
    17341 cgcgttctca ccaaaatcca attagcacgt tctaccgcgt tcaaaggatt gaaatgtttt
    17401 atcctctcca aatgaaatga acttccttat atagaggaag ggtcttgcga aggatagtgg
    17461 gattgtgcgt catcccttac gtcagtggag atatcacatc aatccacttg ctttgaagac
    17521 gtggttggaa cgtcttcttt ttccacgatg ttcctcgtgg gtgggggtcc atctttggga
    17581 ccactgtcgg tagaggcatc ttgaacgata gcctttcctt tatcgcaatg atggcatttg
    17641 tagaagccat cttccttttc tactgtcctt tcgatgaagt gacagatagc tgggcaatgg
    17701 aatccgagga ggtttcccga tattaccctt tgttgaaaag tctcaatagc cctctggtct
    17761 tctgagactg tatctttgat attcttggag tagacgagag tgtcgtgctc caccatgttg
    17821 accatcacat caatccactt gctttgaaga cgtggttgga acgtcttctt tttccacgat
    17881 gttcctcgtg ggtgggggtc catctttggg accactgtcg gtagaggcat cttgaacgat
    17941 agcctttcct ttatcgcaat gatggcattt gtagaagcca tcttcctttt ctactgtcct
    18001 ttcgatgaag tgacagatag ctgggcaatg gaatccgagg aggtttcccg atattaccct
    18061 ttgttgaaaa gtctcaatag ccctctggtc ttctgagact gtatctttga tattcttgga
    18121 gtagacgaga gtgtcgtgct ccaccatgtt gacctgcagg catgcgcaaa tttaaagcgc
    18181 tgatatcgat cgcgcgcaga tctgtcatga tgatcattgc aattggatcc atctccactg
    18241 acgtaaggga tgacgcacaa tcccactatc cttcgcaaga cccttcctct atataaggaa
    18301 gttcatttca tttggagagg ataaaacatt gcacctatgg tgttgccctg gctggggtat
    18361 gtcagtgatc gcagtagaat gtactaattg acaagttgga gaatacggta gaacgtcctt
    18421 atccaacaca gcctttatcc ctctccctga cgaggttttt gtcagtgtaa tatttctttt
    18481 tgaactatcc agcttagtac cgtacgggaa agtgactggt gtgcttatct ttgaaatgtt
    18541 actttgggtt tcggttcttt aggttagtaa gaaagcactt gtcttctcat acaaaggaaa
    18601 acctgagacg tatcgcttac gaaagtagca atgaaagaaa ggtggtggtt ttaatcgcta
    18661 ccgcaaaaac gatggggtcg ttttaattaa cttctcctac gcaagcgtct aaacggacgt
    18721 tggggttttg ctagtttctt tagagaaaac tagctaagtc tttaatgtta tcattagaga
    18781 tggcataaat ataatacttg tgtctgctga taagatcatt ttaatttgga cgattagact
    18841 tgttgaacta caggttactg aatcacttgc gctaatcaac atgggagata tgtacgatga
    18901 atcatttgac aagtcgggcg gtcctgctga cttgatggac gattcttggg tggaatcagt
    18961 ttcgtggaaa gatctgttga agaagttaca cagcataaaa tttgcactac agtctggtag
    19021 agatgagatc actgggttac tagcggcact gaatagacag tgtccttatt caccatatga
    19081 gcagtttcca gataagaagg tgtatttcct tttagactca cgggctaaca gtgctcttgg
    19141 tgtgattcag aacgcttcag cgttcaagag acgagctgat gagaagaatg cagtggcggg
    19201 tgttacaaat attcctgcga atccaaacac aacggttacg acgaaccaag ggagtactac
    19261 tactaccaag gcgaacactg gctcgacttt ggaagaagac ttgtacactt attacaaatt
    19321 cgatgatgcc tctacagctt tccacaaatc tctaacttcg ttagagaaca tggagttgaa
    19381 gagttattac cgaaggaact ttgagaaagt attcgggatt aagtttggtg gagcagctgc
    19441 tagttcatct gcaccgcctc cagcgagtgg aggtccgata cgtcctaatc ccaagcttta
    19501 gggatttaag gacgtgaact ctgttgagat ctctgtgaaa ttcagagggt gggtgatacc
    19561 atattcactg atgccattag cgacatctaa atagggctaa ttgtgactaa tttgagggaa
    19621 tttcctttac cattgacgtc agtgtcgttg gtagcatttg agtttcgcaa tgcacgaatt
    19681 acttaggaag tggcttgacg acactaatgt gttattgtta gataatggtt tggtggtcaa
    19741 ggtacgtagt agagtcccac atattcgcac gtatgaagta attggaaagt tgtcagtttt
    19801 tgataattca ctgggagatg at
//
