LOCUS       sequence-476962-       12234 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Targets the human CLYBL safe harbor locus for integration of 
            dox-inducible NGN2 for differentiation of iPSCs into cortical 
            neurons. Contains a floxed BSD-NLS-mApple selection cassette..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12234)
  AUTHORS   Tian R, Gachechiladze MA, Ludwig CH, Laurie MT, Hong JY, Nathaniel 
            D, Prabhu AV, Fernandopulle MS, Patel R, Abshari M, Ward ME, 
            Kampmann M
  TITLE     CRISPR Interference-Based Platform for Multimodal Genetic Screens in
            Human iPSC-Derived Neurons.
  JOURNAL   Neuron. 2019 Aug 5. pii: S0896-6273(19)30640-3. doi: 
            10.1016/j.neuron.2019.07.014.
  PUBMED    31422865
REFERENCE   2  (bases 1 to 12234)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron. 
            2019 Aug 5. pii: S0896-6273(19)30640-3. doi: 
            10.1016/j.neuron.2019.07.014."
COMMENT     Sequence Label: CLYBL-TO-hNGN2-BSD-mApple
FEATURES             Location/Qualifiers
     source          1..12234
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     266..285
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2052..2429
                     /label=TRE3G promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G"
     protein_bind    2059..2077
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2095..2113
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2131..2149
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2167..2185
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2203..2221
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2239..2257
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2275..2293
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2338..2362
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     polyA_signal    3297..3418
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3334..3353)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3388..3407
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3467..3483)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3467..3483)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3480..3502)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3491..3507
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3515..3521,3522..3539,3540..3545))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3560..3581
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3698..3715)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     4007..4026
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    4025..4080
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     4126..4145
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(4236..4982)
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(5014..5033)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     intron          5041..6057
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     complement(5095..5117)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     promoter        complement(6058..6335)
                     /label=chicken beta-actin promoter
     enhancer        6337..6716
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     polyA_signal    6722..6946
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     6935..6952
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     protein_bind    6968..7001
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      7779..7788
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(7837..7863)
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             complement(7879..7905)
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             complement(7912..7965)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     LTR             8434..8702
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     promoter        complement(8715..8926)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     polyA_signal    8962..9186
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(9191..9991)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="GPRIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQG
                     RPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEE
                     HQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQD
                     IALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9302..9321)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     9912..9931
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     CDS             complement(9986..10039)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     misc_feature    10063..10088
                     /label=SA
                     /note="splice acceptor site"
     protein_bind    10089..10122
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             join(10980..11048,11049..11840)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11198..11217)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(12011..12234,1..365)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
       61 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
      121 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
      181 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
      241 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
      301 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
      361 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc gaattcgccc ttagagcaga
      421 gccaggaacc cctgtaggga aggggcagga gagccagggg catgagatgg tggacgagga
      481 agggggacag ggaagcctga gcgcctctcc tgggcttgcc aaggactcaa acccagaagc
      541 ccagagcagg gccttaggga agcgggacgg ctctgttgga gagtccagta ttgaattagt
      601 gatagataca cagaaaaaaa gcaaattgaa taggtgaatg gataaacaaa ctgtggaaca
      661 tccagacaat gaaatattca ccaataaaaa gaaatgagct atcaagccac attggctatg
      721 cccttcacat ttaaggagga accttaaatg catattgcca aattttaaaa gctagtctga
      781 aaatgctaca gactgtatga ttccaattat atgacatttt agaaaaggca aaactataga
      841 gatcagtggt tgccagcggc tcatggaggc aggatgaatt ggtggaacac agggatgctt
      901 tcagggtaat gaaaatatct tgtgtgatcc tgtaataatg gatacgtggc ataatgcatt
      961 tgttaaaacc tgtaaactag ccaggtatag tggctttttt gcacctatag tctcagctac
     1021 ttggtaggct gagacaggaa gatcacttga gcccaggact tcaagaccag cctgggtaat
     1081 atagtaagac cccatctcta ttttttaaag aaattaagaa agaaaagaaa cccatcaact
     1141 gtataacaca aagaatcgta atgtaaacta cagtcctgag taatgaaaca tttttaaaga
     1201 aataaataaa ttcaaggcaa ttattaactt caggaaaaac agtaaagtaa acataagtat
     1261 acaaatcaaa catggctcag ttgtgaaaat atttacaagg tcactttaag taacattgaa
     1321 tgttgatata atcccaaaat atatttatgt tggaaggatg aggagattta attaagggag
     1381 gtggtccctg cagttacgcc aatgataacc cccgccagaa aaatcttagt agccttccct
     1441 ttttgttttc cgtgccccaa ctcggcggat tgactcggcc ccttccggaa acacccgaat
     1501 caacttctag tcaaattatt gttcacgccg caatgaccca cccctggccc gcgtctgtgg
     1561 aactgacccc tggtgtacag gagagttcgc tgctgaaagt ggtcccaaag gggtactagt
     1621 ttttaagctc ccaactcccc ctcccccagc gtctggagga ttccacaccc tcgcaccgca
     1681 ggggcgagga agtgggcgga gtccggtttt ggcgccagcc gctgaggctg ccaagcagaa
     1741 aagccaccgc tgaggagact ccggtcactg tcctcgcccc gcctccccct tccctcccct
     1801 tggggaccac cgggcgccac gccgcgaacg gtaagtgccg cggtcgtcgg cgcctccgcc
     1861 ctccccctag ggccccaatt cccagcgggc gcggcgcgcg gcccctcccc ccgccgggcg
     1921 cgcgcccgct gccccgccct tcgtggccgc ccggcgtggg cggtgccacc cctccccccg
     1981 gcggccccgc gcgcagctcc cggctccctc ccccttcgga tgtggcttga gctgtaggcg
     2041 cggagggtac cagtttactc cctatcagtg atagagaacg tatgaagagt ttactcccta
     2101 tcagtgatag agaacgtatg cagactttac tccctatcag tgatagagaa cgtataagga
     2161 gtttactccc tatcagtgat agagaacgta tgaccagttt actccctatc agtgatagag
     2221 aacgtatcta cagtttactc cctatcagtg atagagaacg tatatccagt ttactcccta
     2281 tcagtgatag agaacgtata agctttaggc gtgtacggtg ggcgcctata aaagcagagc
     2341 tcgtttagtg aaccgtcaga tcgcctggag caattccaca acacttttgt cttataccaa
     2401 ctttccgtac cacttcctac cctcgtaaag gatccgccgc caccatgttc gtcaaatccg
     2461 agaccttgga gttgaaggag gaagaggacg tgttagtgct gctcggatcg gcctcccccg
     2521 ccttggcggc cctgaccccg ctgtcatcca gcgccgacga agaagaggag gaggagccgg
     2581 gcgcgtcagg cggggcgcgt cggcagcgcg gggctgaggc cgggcagggg gcgcggggcg
     2641 gcgtggctgc gggtgcggag ggctgccggc ccgcacggct gctgggtctg gtacacgatt
     2701 gcaaacggcg cccttcccgg gcgcgggccg tctcccgagg cgccaagacg gccgagacgg
     2761 tgcagcgcat caagaagacc cgtagactga aggccaacaa ccgcgagcga aaccgcatgc
     2821 acaacctcaa cgcggcactg gacgcgctgc gcgaggtgct ccccacgttc cccgaggacg
     2881 ccaagctcac caagatcgag accctgcgct tcgcccacaa ctacatctgg gcactcaccg
     2941 agaccctgcg cctggcggat cactgcgggg gcggcggcgg gggcctgccg ggggcgctct
     3001 tctccgaggc agtgttgctg agcccgggag gcgccagcgc cgccctgagc agcagcggag
     3061 acagcccctc gcccgcctcc acgtggagtt gcaccaacag ccccgcgccg tcctcctccg
     3121 tgtcctccaa ttccacctcc ccctacagct gcactttatc gcccgccagc ccggccgggt
     3181 cagacatgga ctattggcag cccccacctc ccgacaagca ccgctatgca cctcacctcc
     3241 ccatagccag ggattgtatc ttgtaagttt aaactcgcga atcgatcgcc caccccaact
     3301 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     3361 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     3421 atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc
     3481 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     3541 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     3601 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     3661 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     3721 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggacggccgc acatgtaagg
     3781 aaaattttag ggatgttaaa gaaaaaaata acacaaaaca aaatataaaa aaaatctaac
     3841 ctcaagtcaa ggcttttcta tggaataagg aatggacagc agggggctgt ttcatatact
     3901 gatgacctct ttatagccaa cctttgttca tggcagccag catatgggca tatgttgcca
     3961 aactctaaac caaatactca ttctgatgtt ttaaatgatt tgccctccca tatgtccttc
     4021 cgagtgagag acacaaaaaa ttccaacaca ctattgcaat gaaaataaat ttcctttatt
     4081 agccagaagt cagatgctca aggggcttca tgatgtcccc ataatttttg gcagagggaa
     4141 aaagatctca gtggtatttg tgagccaggg cattggccac accagtcacc accttctgat
     4201 aggcagcctg cacctgagga gtgaattcac gcgttttagc ctggcagcat atcgagatcg
     4261 aagtcatcga gagcgtcagc agggagcata tccagatcaa aatcatccag ggcatcggcg
     4321 ggcagcatgt ccaggtcgaa gtcgtccagg gcgtcggtgg gtccgccgct ctcgcacttc
     4381 agctgcttct cgaggccgca gatgatcagt tccaggccga acaggaaggc gggctcggcg
     4441 ccctgtctgt cgaacagctc gatggcctgc ttcagcaggg ggggcatgct gtcggtggtg
     4501 ggtgtctctc tttcctcttt ggcgacctgg tgttcctgtt cttccagcac gcagcccagg
     4561 gtgaagtggc ccacggcgct cagggcgtac agggcgtttt ccaggctgaa gccctgctgg
     4621 cacaggaagg ccagctggtt ttccagtgtc tcgtactgct tctcggtggg tctggtgccc
     4681 aggtgcactt tggcgccgtc ccggtggctc agcagggcgc atctgtagct cttggcgttg
     4741 ttccgcagga agtcctgcca gctctcgcct tccagagggc agctgtgggt gtggtgccgg
     4801 tccagcatct cgatgggcag ggcgtccagc agggcccgct tgttcttcac gtgccagtac
     4861 agggtgggct gttccacgcc cagtttctgg gccagcttcc gggtggtcag gccctcgatg
     4921 cccacgccgt tcagcagttc cagggcgctg ttgatcactt tgctcttgtc cagccggctc
     4981 atgtcgacat tctttgccaa aatgatgaga cagcacaata accagcacgt tgcccaggag
     5041 ctgtaggaaa aagaagaagg catgaacatg gttagcagag gctctagagc cgccggtcac
     5101 acgccagaag ccgaaccccg ccctgccccg tcccccccga aggcagccgt ccccccgcgg
     5161 acagccccga ggctggagag ggagaagggg acggcggcgc ggcgacgcac gaaggccctc
     5221 cccgcccatt tccttcctgc cggcgccgca ccgcttcgcc ccgcgcccgc tagagggggt
     5281 gcggcggcgc ctcccagatt tcggctccgc acagatttgg gacaaaggaa gtccctgcgc
     5341 cctctcgcac gattaccata aaaggcaatg gctgcggctc gccgcgcctc gacagccgcc
     5401 ggcgctccgg gggccgccgc gccccycccc cgagcccycc ccggcccgrg gcggccccgc
     5461 cccgcccggc acccccmcct gccgccaccc cccgcccggc acggcgagcc ccgcgccacg
     5521 ccccgtacgg agccccgcac ccgaagccgg gccgtgctca gcaactcggg gaggggggtg
     5581 cagggggggt tgcagcccga ccgacgcgcc cacaccccct gctcaccccc ccacgcacac
     5641 accccgcacg cagcctttgt tcccctcgca gccccccccg caccgcgggg caccgccccc
     5701 ggccgcgctc ccctcgcgca cactgcggag cgcacaaagc cccgcgccgc gcccgcagcg
     5761 ctcacagccg ccgggcagcg cggagccgca cgcggcgctc cccacgcaca cacacacgca
     5821 cgcacccccc gagccgctcc ccccgcacaa agggccctcc cggagcccct caaggctttc
     5881 acgcagccac agaaaagaaa caagccgtca ttaaaccaag cgctaattac agcccggagg
     5941 agaagggccg tcccgcccgc tcacctgtgg gagtaacgcg gtcagtcaga gccggggcgg
     6001 gcggcgcgag gcggcggcgg agcggggcac ggggcgaagg cagcgcgcag cgactcccgc
     6061 ccgccgcgcg cttcgctttt tatagggccg ccgccgccgc cgcctcgcca taaaaggaaa
     6121 ctttcggagc gcgccgctct gattggctgc cgccgcacct ctccscctcs ccccsccccs
     6181 cccctcsccc cccccccccc cccctggcgc gcgccccccc cccccccccg cccccatcgc
     6241 tgcacaaaat aattaaaaaa taaataaata caaaattggg ggtggggagg ggggggagat
     6301 ggggagagtg aagcagaacg tggggctcac ctcgaccatg gtaatagcga tgactaatac
     6361 gtagatgtac tgccaagtag gaaagtccca taaggtcatg tactgggcat aatgccaggc
     6421 gggccattta ccgtcattga cgtcaatagg gggcgtactt ggcatatgat acacttgatg
     6481 tactgccaag tgggcagttt accgtaaata ctccacccat tgacgtcaat ggaaagtccc
     6541 tattggcgtt actatgggaa catacgtcat tattgacgtc aatgggcggg ggtcgttggg
     6601 cggtcagcca ggcgggccat ttaccgtaag ttatgtaacg cggaactcca tatatgggct
     6661 atgaactaat gaccccgtaa ttgattacta ttaataacta gtcaataatc aatgtcgaga
     6721 cccatagagc ccaccgcatc cccagcatgc ctgctattgt cttcccaatc ctcccccttg
     6781 ctgtcctgcc ccaccccacc ccccagaata gaatgacacc tactcagaca atgcgatgca
     6841 atttcctcat tttattagga aaggacagtg ggagtggcac cttccagggt caaggaaggc
     6901 acgggggagg ggcaaacaac agatggctgg caactagaag gcacagtcga ggctgatcag
     6961 cgggtttata acttcgtata atgtatgcta tacgaagtta tggtacctta ctcgtccatg
     7021 ccgccggtgg agtggcggcc ctcggcgcgt tcgtactgtt ccacgatggt gttacttgta
     7081 cagctcgtcc attccgcctg tgctgtgtct gccttcggct ctctcgtact gttccacgat
     7141 ggtgtagtcc tcgttgtggg acacgatgtc cagcttgata tccacgatgt aggcgccagg
     7201 cagctgcacg ggtttcttgg ccttgtaggt ggttttcact tcggcggcat agtggccgcc
     7261 atccttcagc ttcagccgct tcttgatctc ggacttcagg gcgccatcct cagggtacat
     7321 tctttcctcg ctggcttccc agcccatggt ctttttctgc atcacggggc catcagaggg
     7381 aaagttggtg ccccgcagct tcactttgta gatgaacacg ccgtcctgca gagagctatc
     7441 ctggttcacg tggatgatgc cgccgtcctc gaagttcatc actctctccc atctgaagcc
     7501 ctcggggaag ctcagcttga agtagtcggg aatgtcggcg gggtgcttga tgtacacctt
     7561 gctgccgtac ataaactgag ggctcagaat gtcccaggca aaaggcagag ggccgccttt
     7621 ggtcactttc agcttggcgg tctgaaaagc ctcgtagggt ctgccctcgc cttcgccttc
     7681 aatctcgaac tcgtggccgt tcacgctgcc ttccatgtgc accttgaacc gcatgaattc
     7741 tttgatgatg gccatgttgt tctcttcgcc cttggacacc atggtggcga ccggtagcgt
     7801 ggcgaccggg gtggcggtgg ctccccctga ccctccatcc aacttaacgc gctttgcagc
     7861 aggacctcca gaaccgccgt cgagtttgac ccgtttagcg gcagggaccg gtgggccagg
     7921 attctcctcg acgtcaccgc atgttagcag acttcctctg ccctctcccg atcccgatcc
     7981 atttcgggta tatttgagtg gaatgagttc ttcaatcgta gttttgacta acttgccatt
     8041 catttctatt aacacaaaac aatctggtgc atagtctgaa atcaactccc tacacatacc
     8101 acaaggactt accactcgaa tacttctatc tacttcgtca gaataagggt gtctaacagc
     8161 tacaatcgtg tcaaaatcct tttgtccatt cgaaactgca ctaccaatcg caatggcttc
     8221 tgcacaaaca gttactcgtc ctatatacgc ttcaatatgt actgccgaaa tgatttctcc
     8281 tgttttcgta cgaattgccg ctcccacatg atgtttatta tcctcataaa gcattgtaat
     8341 cttctctgtc gctacttcta ctaattctag atcctgctgg gagatgttga aggtcttcat
     8401 ggtggcgcta gcgtaggcgc cggtcacagc ttggatctgt aacggcgcag aacagaaaac
     8461 gaaacaaaga cgtagagttg agcaagcagg gtcaggcaaa gcgtggagag ccggctgagt
     8521 ctaggtaggc tccaagggag cgccggacaa aggcccggtc tcgacctgag ctttaaactt
     8581 acctagacgg cggacgcagt tcaggaggca ccacaggcgg gaggcggcag aacgcgactc
     8641 aaccggcgtg gatggcggcc tcaggtaggg cggcgggcgc gtgaaggaga gatgcgagcc
     8701 cctcgaagct tcagctgtgt tctggcggca aacccgttgc gaaaaagaac gttcacggcg
     8761 actactgcac ttatatacgg ttctccccca ccctcgggaa aaaggcggag ccagtacacg
     8821 acatcacttt cccagtttac cccgcgccac cttctctagg caccggttca attgccgacc
     8881 cctcccccca acttctcggg gactgtgggc gatgtgcgct ctgcccactg acgggcaccg
     8941 gagcgatcgc agatcatgca tccatagagc ccaccgcatc cccagcatgc ctgctattgt
     9001 cttcccaatc ctcccccttg ctgtcctgcc ccaccccacc ccccagaata gaatgacacc
     9061 tactcagaca atgcgatgca atttcctcat tttattagga aaggacagtg ggagtggcac
     9121 cttccagggt caaggaaggc acgggggagg ggcaaacaac agatggctgg caactagaag
     9181 gcacaggttt ttagaagaac tcgtcaagaa ggcgatagaa ggcgatgcgc tgcgaatcgg
     9241 gagcggcgat accgtaaagc acgaggaagc ggtcagccca ttcgccgcca agctcttcag
     9301 caatatcacg ggtagccaac gctatgtcct gatagcggtc cgccacaccc agccggccac
     9361 agtcgatgaa tccagaaaag cggccatttt ccaccatgat attcggcaag caggcatcgc
     9421 catgggtcac gacgagatcc tcgccgtcgg gcatgcgcgc cttgagcctg gcgaacagtt
     9481 cggctggcgc gagcccctga tgctcttcgt ccagatcatc ctgatcgaca agaccggctt
     9541 ccatccgagt acgtgctcgc tcgatgcgat gtttcgcttg gtggtcgaat gggcaggtag
     9601 ccggatcaag cgtatgcagc cgccgcattg catcagccat gatggatact ttctcggcag
     9661 gagcaaggtg agatgacagg agatcctgcc ccggcacttc gcccaatagc agccagtccc
     9721 ttcccgcttc agtgacaacg tcgagcacag ctgcgcaagg aacgcccgtc gtggccagcc
     9781 acgatagccg cgctgcctcg tcctgcagtt cattcagggc accggacagg tcggtcttga
     9841 caaaaagaac cgggcgcccc tgcgctgaca gccggaacac ggcggcatca gagcagccga
     9901 ttgtctgttg tgcccagtca tagccgaata gcctctccac ccaagcggcc ggagaacctg
     9961 cgtgcaatcc atcttgttca atcctagggc cgggattctc ctccacgtca ccgcatgtta
    10021 gaagacttcc tctgccctct ccgctgccag atctctcgag gccctgtggg aggaagagaa
    10081 gaggtcagat aacttcgtat aatgtatgct atacgaagtt atgcggccgc tgggagaagg
    10141 gtaagagagc tcaatcctca tcttacatag aaggaagtca gtagataata tcttacattt
    10201 aaaagtcaag aaataacagt ctgaccatac tatctagaaa tatggaagtt aacagtaagg
    10261 aagttaaaac agttgaaagt agtttcgtct agaaagaaag acttggggaa gaacaaagaa
    10321 ggctaatttt cattatgagc tttattgtaa cagaaaagag accactaact cagctaggtt
    10381 ttcccaattc ctcaacactt tggggcacag tgtttagtca gagctgtgat cactctctga
    10441 gcacatggat tgtatcttaa ttttcatctc attgaaaata tgtaccattt gtccaagtgg
    10501 aaactgggct gaagcatgac tgtgaccacc accaaagaaa tggctagact cgctaaggtc
    10561 tgctggggtg acccgtgatt gtgttgtgct tgatcttgtt gagcatggct cttttaagtc
    10621 accatacctg cccctgccaa tgcaccaaac accaacttaa agggactttc agcttttgta
    10681 agtcagtctc agccttactt ctgaaccatt tctttgactt ctttcatgac tctgatcctg
    10741 tcccagtttt aagcctccca gccaccagtc atgctgtttt gctctgctat ttacattctt
    10801 ctctacctac tttggacttg atggtatgcc tactatatta gttatttatt gctgtgtaac
    10861 aaaccactct gaaacatagt ggcttacaac cacaaccatt ttattttatt atgaaactgt
    10921 gggtcagaat gtatattggc ccattggcat gattctcctg ctattgaaaa aggaagagta
    10981 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
    11041 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
    11101 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
    11161 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
    11221 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
    11281 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
    11341 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
    11401 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
    11461 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
    11521 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
    11581 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
    11641 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
    11701 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
    11761 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
    11821 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
    11881 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
    11941 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
    12001 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
    12061 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
    12121 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
    12181 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcc
//
