LOCUS       sequence-47697-e         800 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Young CL, Raden DL, Caplan JL, Czymmek KJ, Robinson AS
  TITLE     Cassette series designed for live-cell imaging of proteins and 
            high-resolution techniques in yeast.
  JOURNAL   Yeast. 2012 Mar;29(3-4):119-36. doi: 10.1002/yea.2895. Epub 2012 Apr
            4.
  PUBMED    22473760
REFERENCE   2  (bases 1 to 800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast."; 
            date: "2012-03"; volume: "29(3-4):119-36. doi"; pages: " 
            10.1002/yea.2895. Epub 2012 Apr 4"
COMMENT     Sequence Label: pCY 3080-01
FEATURES             Location/Qualifiers
     source          1..800
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(28..744)
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSRLSKDPNEKRDHMVLLE
                     FVTAAGITHGIDELYK"
ORIGIN
        1 aattcgctta tttagaagtg gcgcgcctta tttgtacaat tcatcaatac catgggtaat
       61 accagcagca gtaacaaatt ctaacaagac catgtggtct ctcttttcgt ttggatcttt
      121 ggataatcta gattgatagg ataagtaatg gttgtctggt aacaagactg gaccatcacc
      181 aattggagta ttttgttgat aatggtcagc taattgaaca gaaccatctt caatgttgtg
      241 tctaattttg aagttaactt tgataccatt cttttgtttg tcagccatga tgtaaacatt
      301 gtgagagtta tagttgtatt ccaatttgtg acctaaaatg ttaccatctt ctttaaaatc
      361 aatacctttt aattcgattc tattaactaa ggtatcacct tcaaacttga cttcagctct
      421 ggtcttgtag ttaccgtcat ctttgaaaaa aatagttctt tcttgaacat aaccttctgg
      481 catggcagac ttgaaaaagt catgttgttt catatgatct gggtatctag caaaacacat
      541 caaaccataa cctaaagtag tgactaaggt tggccatgga actggcaatt taccagtagt
      601 acaaataaat tttaaggtca atttaccgta agtagcatca ccttcacctt caccggagac
      661 agaaaatttg tgaccattaa catcaccatc taattcaacc aaaattggga caacaccagt
      721 gaataattct tcacctttag acatgttaat taaaccagca ccgtcaccgt taattaaccc
      781 ggggatccgt cgacctgcag
//
