LOCUS       sequence-476979-       14190 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Super folding Green Fluorescence Protein (sfGFP).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14190)
  AUTHORS   Liu Y, Rajput R, Islam MT, Valle ID, Yao T, Agrawal R, Boone BA, 
            Eckert CA, Abraham PE, Chen JG, Tuskan GA, Yang X
  TITLE     A split ribozyme system for in vivo plant RNA imaging and genetic 
            engineering.
  JOURNAL   Plant Biotechnol J. 2025 May;23(5):1640-1649. doi: 
            10.1111/pbi.14612. Epub 2025 Feb 7.
  PUBMED    39919021
REFERENCE   2  (bases 1 to 14190)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/pbi.14612";
            journalName: "Plant Biotechnol J"; date: "2025-05"; volume: "23"; 
            issue: "5"; pages: "1640-1649"
COMMENT     Sequence Label: pXYB033
FEATURES             Location/Qualifiers
     source          1..14190
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(16..361)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     723..745
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     737..754
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     738..754
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1269..1614
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             join(1739..1741,1742..2455)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             1739..2452
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Nager et al., 2011)"
                     /label=superfolder GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(1769..1797)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     2392..2412
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     terminator      2457..2705
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     primer_bind     complement(3395..3411)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3395..3411)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3408..3430)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3419..3435
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3443..3449,3450..3467,3468..3473))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3488..3509
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        3700..4376
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     CDS             4444..5241
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLL
                     SSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEH
                     QGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDI
                     ALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4501..4520)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5111..5130
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5281..5455
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    5533..5557
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             5982..6776
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      6863..7451
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7352..7371
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7605..7622
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7637..7777
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7703..7722)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7863..7885
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8121..8315
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8381..9454)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(9883..10512)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    11813..11837
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     terminator      complement(11859..12111)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(12143..12160)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(join(12167..13984,14175..14189))
                     /codon_start=1
                     /product="beta-glucuronidase"
                     /label=GUSPlus(TM)
                     /note="codon-optimized Staphylococcus gusA gene with a 
                     catalase intron to ensure expression in plants but not 
                     bacteria"
                     /translation="MVDLRNRRTSLYPINTETRGVFDLNGVWNFKLDYGKGLEEKWYES
                     KLTDTISMAVPSSYNDIGVTKEIRNHIGYVWYEREFTVPAYLKDQRIVLRFGSATHKAI
                     VYVNGELVVEHKGGFLPFEAEINNSLRDGMNRVTVAVDNILDDSTLPVGLYSERHEEGL
                     GKVIRNKPNFDFFNYAGLHRPVKIYTTPFTYVEDISVVTDFNGPTGTVTYTVDFQGKAE
                     TVKVSVVDEEGKVVASTEGLSGNVEIPNVILWEPLNTYLYQIKVELVNDGLTIDVYEEP
                     FGVRTVEVNDGKFLINNKPFYFKGFGKHEDTPINGRGFNEASNVMDFNILKWIGANSFR
                     TAHYPYSEELMRLADREGLVVIDETPAVGVHLNFMATTGLGEGSERVSTWEKIRTFEHH
                     QDVLRELVSRDKNHPSVVMWSIANEAATEEEGAYEYFKPLVELTKELDPQKRPVTIVLF
                     VMATPETDKVAELIDVIALNRYNGWYFDGGDLEAAKVHLRQEFHAWNKRCPGKPIMITE
                     YGADTVAGFHDIDPVMFTEEYQVEYYQANHVVFDEFENFVGEQAWNFADFATSQGVMRV
                     QGNKKGVFTRDRKPKLAAHVFRERWTNIPDFGYKN"
     intron          13985..14174
                     /label=cat1 intron
                     /note="castor bean catalase intron, modified"
ORIGIN
        1 gtcaagagtc ccccgtgttc tctccaaatg aaatgaactt ccttatatag aggaagggtc
       61 ttgcgaagga tagtgggatt gtgcgtcatc ccttacgtca gtggagatat cacatcaatc
      121 cacttgcttt gaagacgtgg ttggaacgtc ttctttttcc acgatgctcc tcgtgggtgg
      181 gggtccatct ttgggaccac tgtcggcaga ggcatcttca acgatggcct ttcctttatc
      241 gcaatgatgg catttgtagg agccaccttc cttttccact atcttcacaa taaagtgaca
      301 gatagctggg caatggaatc cgaggaggtt tccggatatt accctttgtt gaaaagtctc
      361 aattgccctt tggtcttctg agactgtatc tttgatattt ttggagtaga caagtgtgtc
      421 gtgctccacc atgttgacga agattttctt cttgtcattg agtcgtaaga gactctgtat
      481 gaactgttcg ccagtcttta cggcgagttc tgttaggtcc tctatttgaa tctttgactc
      541 catgaagcta aactgaaggc gggaaacgac aatctgatcc aagctcaagc tgctctagca
      601 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
      661 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt
      721 ttcccagtca cgacgttgta aaacgacggc cagtgccaag cttgcatgcc tgcaggtccc
      781 cagattagcc ttttcaattt cagaaagaat gctaacccac agatggttag agaggcttac
      841 gcagcaggtc tcatcaagac gatctacccg agcaataatc tccaggaaat caaatacctt
      901 cccaagaagg ttaaagatgc agtcaaaaga ttcaggacta actgcatcaa gaacacagag
      961 aaagatatat ttctcaagat cagaagtact attccagtat ggacgattca aggcttgctt
     1021 cacaaaccaa ggcaagtaat agagattgga gtctctaaaa aggtagttcc cactgaatca
     1081 aaggccatgg agtcaaagat tcaaatagag gacctaacag aactcgccgt aaagactggc
     1141 gaacagttca tacagagtct cttacgactc aatgacaaga agaaaatctt cgtcaacatg
     1201 gtggagcacg acacacttgt ctactccaaa aatatcaaag atacagtctc agaagaccaa
     1261 agggcaattg agacttttca acaaagggta atatccggaa acctcctcgg attccattgc
     1321 ccagctatct gtcactttat tgtgaagata gtggaaaagg aaggtggctc ctacaaatgc
     1381 catcattgcg ataaaggaaa ggccatcgtt gaagatgcct ctgccgacag tggtcccaaa
     1441 gatggacccc cacccacgag gagcatcgtg gaaaaagaag acgttccaac cacgtcttca
     1501 aagcaagtgg attgatgtga tatctccact gacgtaaggg atgacgcaca atcccactat
     1561 ccttcgcaag acccttcctc tatataagga agttcatttc atttggagag aacacggggg
     1621 actcaaacat tactcattca caaaaccatc ttaaagcaac tacacaagtc ttgaaatttt
     1681 ctcatatttt ctatttacta tataaacttt taatcaaatc aagattaaag ttaattaaat
     1741 gagcaaagga gaagaacttt tcactggagt tgtcccaatt cttgttgaat tagatggtga
     1801 tgttaatggg cacaaatttt ctgtccgtgg agagggtgaa ggtgatgcta caaacggaaa
     1861 actcaccctt aaatttattt gcactactgg aaaactacct gttccgtggc caacacttgt
     1921 cactactctg acctatggtg ttcaatgctt ttcccgttat ccggatcaca tgaaacggca
     1981 tgactttttc aagagtgcca tgcccgaagg ttatgtacag gaacgcacta tatctttcaa
     2041 agatgacggg acctacaaga cgcgtgctga agtcaagttt gaaggtgata cccttgttaa
     2101 tcgtatcgag ttaaagggta ttgattttaa agaagatgga aacattcttg gacacaaact
     2161 cgagtacaac tttaactcac acaatgtata catcacggca gacaaacaaa agaatggaat
     2221 caaagctaac ttcaaaattc gccacaacgt tgaagatggt tccgttcaac tagcagacca
     2281 ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt ttaccagaca accattacct
     2341 gtcgacacaa tctgtccttt cgaaagatcc caacgaaaag cgtgaccaca tggtccttct
     2401 tgagtttgta actgctgctg ggattacaca tggcatggat gagctctaca aataaatatg
     2461 aagatgaaga tgaaatattt ggtgtgtcaa ataaaaagct tgtgtgctta agtttgtgtt
     2521 tttttcttgg cttgttgtgt tatgaatttg tggctttttc taatattaaa tgaatgtaag
     2581 atctcattat aatgaataaa caaatgtttc tataatccat tgtgaatgtt ttgttggatc
     2641 tcttctgcag catataacta ctgtatgtgc tatggtatgg actatggaat atgattaaag
     2701 ataagatggg ctcatagagt aaaacgaggc gagggaccta taaacctccc ttcatcatgc
     2761 tatttcatga tctattttat aaaataaaga tgtagaaaaa agtaagcgta ataaccgcaa
     2821 aacaaatgat ttaaaacatg gcacataatg aggagattaa gttcggttta cgtttatttt
     2881 agtactaatt gtaacgtgag actacgtatc gggaatcgcc taattaaagc attaatgcga
     2941 acctgattag attcaccgac cctcctatcg tgtcgacctt tctgtttctt agaatttttt
     3001 ggtagtctat gtactaataa tgtcagcttc gtatttattt cataagcaat ttgcatttgc
     3061 aatttgtttt ttacttttat ttttattgta ttgtggaatg tggactcgta ccaacatgaa
     3121 gttatatacc accaaaaaaa ttacagttag tcaaaagatt cacgagtgag agctacttat
     3181 gattgtcttt tacgtatatg tctaattgtc tatttgctca ataatctttg tactttcttt
     3241 tgtcgttgat aaaatcacaa agttccaaaa gtaatcgaat gatttgcttt taagaaaaga
     3301 agagctcaat aattcaacat atatctgtac acaattaccg cctttgagtg agctctagat
     3361 gccacctgac gtctaagaag aattcgtaat catggtcata gctgtttcct gtgtgaaatt
     3421 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     3481 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     3541 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     3601 tgcgtattgg ctagagcagc ttgccaacat ggtggagcac gacactctcg tctactccaa
     3661 gaatatcaaa gatacagtct cagaagacca aagggctatt gagacttttc aacaaagggt
     3721 aatatcggga aacctcctcg gattccattg cccagctatc tgtcacttca tcaaaaggac
     3781 agtagaaaag gaaggtggca cctacaaatg ccatcattgc gataaaggaa aggctatcgt
     3841 tcaagatgcc tctgccgaca gtggtcccaa agatggaccc ccacccacga ggagcatcgt
     3901 ggaaaaagaa gacgttccaa ccacgtcttc aaagcaagtg gattgatgtg aacatggtgg
     3961 agcacgacac tctcgtctac tccaagaata tcaaagatac agtctcagaa gaccaaaggg
     4021 ctattgagac ttttcaacaa agggtaatat cgggaaacct cctcggattc cattgcccag
     4081 ctatctgtca cttcatcaaa aggacagtag aaaaggaagg tggcacctac aaatgccatc
     4141 attgcgataa aggaaaggct atcgttcaag atgcctctgc cgacagtggt cccaaagatg
     4201 gacccccacc cacgaggagc atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc
     4261 aagtggattg atgtgatatc tccactgacg taagggatga cgcacaatcc cactatcctt
     4321 cgcaagaccc ttcctctata taaggaagtt catttcattt ggagaggaca cgctgaaatc
     4381 accagtctct ctctacaaat ctatctctct cgagctttcg cagatcccgg ggggcaatga
     4441 gatatgggga ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg
     4501 ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg
     4561 ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat
     4621 gaactccagg acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca
     4681 gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg
     4741 gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat
     4801 gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa
     4861 catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg
     4921 gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg
     4981 cccgacggcg aggatctcgt cgtgacacat ggcgatgcct gcttgccgaa tatcatggtg
     5041 gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat
     5101 caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac
     5161 cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc
     5221 cttcttgacg agttcttctg aagtagatgc cgaccggatc tgtcgatcga caagctcgag
     5281 tttctccata ataatgtgtg agtagttccc agataaggga attagggttc ctatagggtt
     5341 tcgctcatgt gttgagcata taagaaaccc ttagtatgta tttgtatttg taaaatactt
     5401 ctatcaataa aatttctaat tcctaaaacc aaaatccagt actaaaatcc agatcccccg
     5461 aattaattcg gcgttaattc agtacattaa aaacgtccgc aatgtgttat taagttgtct
     5521 aagcgtcaat ttgtttacac cacaatatat cctgccacca gccagccaac agctccccga
     5581 ccggcagctc ggcacaaaat caccactcga tacaggcagc ccatcagtcc gggacggcgt
     5641 cagcgggaga gccgttgtaa ggcggcagac tttgctcatg ttaccgatgc tattcggaag
     5701 aacggcaact aagctgccgg gtttgaaaca cggatgatct cgcggagggt agcatgttga
     5761 ttgtaacgat gacagagcgt tgctgcctgt gatcaccgcg gtttcaaaat cggctccgtc
     5821 gatactatgt tatacgccaa ctttgaaaac aactttgaaa aagctgtttt ctggtattta
     5881 aggttttaga atgcaaggaa cagtgaattg gagttcgtct tgttataatt agcttcttgg
     5941 ggtatcttta aatactgtag aaaagaggaa ggaaataata aatggctaaa atgagaatat
     6001 caccggaatt gaaaaaactg atcgaaaaat accgctgcgt aaaagatacg gaaggaatgt
     6061 ctcctgctaa ggtatataag ctggtgggag aaaatgaaaa cctatattta aaaatgacgg
     6121 acagccggta taaagggacc acctatgatg tggaacggga aaaggacatg atgctatggc
     6181 tggaaggaaa gctgcctgtt ccaaaggtcc tgcactttga acggcatgat ggctggagca
     6241 atctgctcat gagtgaggcc gatggcgtcc tttgctcgga agagtatgaa gatgaacaaa
     6301 gccctgaaaa gattatcgag ctgtatgcgg agtgcatcag gctctttcac tccatcgaca
     6361 tatcggattg tccctatacg aatagcttag acagccgctt agccgaattg gattacttac
     6421 tgaataacga tctggccgat gtggattgcg aaaactggga agaagacact ccatttaaag
     6481 atccgcgcga gctgtatgat tttttaaaga cggaaaagcc cgaagaggaa cttgtctttt
     6541 cccacggcga cctgggagac agcaacatct ttgtgaaaga tggcaaagta agtggcttta
     6601 ttgatcttgg gagaagcggc agggcggaca agtggtatga cattgccttc tgcgtccggt
     6661 cgatcaggga ggatatcggg gaagaacagt atgtcgagct attttttgac ttactgggga
     6721 tcaagcctga ttgggagaaa ataaaatatt atattttact ggatgaattg ttttagtacc
     6781 tagaatgcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     6841 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     6901 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     6961 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     7021 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     7081 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     7141 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     7201 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     7261 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     7321 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     7381 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     7441 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     7501 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     7561 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     7621 aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac
     7681 accgcatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata
     7741 cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc
     7801 tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt
     7861 ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcaggg
     7921 tgccttgatg tgggcgccgg cggtcgagtg gcgacggcgc ggcttgtccg cgccctggta
     7981 gattgcctgg ccgtaggcca gccatttttg agcggccagc ggccgcgata ggccgacgcg
     8041 aagcggcggg gcgtagggag cgcagcgacc gaagggtagg cgctttttgc agctcttcgg
     8101 ctgtgcgctg gccagacagt tatgcacagg ccaggcgggt tttaagagtt ttaataagtt
     8161 ttaaagagtt ttaggcggaa aaatcgcctt ttttctcttt tatatcagtc acttacatgt
     8221 gtgaccggtt cccaatgtac ggctttgggt tcccaatgta cgggttccgg ttcccaatgt
     8281 acggctttgg gttcccaatg tacgtgctat ccacaggaaa gagacctttt cgaccttttt
     8341 cccctgctag ggcaatttgc cctagcatct gctccgtaca ttaggaaccg gcggatgctt
     8401 cgccctcgat caggttgcgg tagcgcatga ctaggatcgg gccagcctgc cccgcctcct
     8461 ccttcaaatc gtactccggc aggtcatttg acccgatcag cttgcgcacg gtgaaacaga
     8521 acttcttgaa ctctccggcg ctgccactgc gttcgtagat cgtcttgaac aaccatctgg
     8581 cttctgcctt gcctgcggcg cggcgtgcca ggcggtagag aaaacggccg atgccgggat
     8641 cgatcaaaaa gtaatcgggg tgaaccgtca gcacgtccgg gttcttgcct tctgtgatct
     8701 cgcggtacat ccaatcagct agctcgatct cgatgtactc cggccgcccg gtttcgctct
     8761 ttacgatctt gtagcggcta atcaaggctt caccctcgga taccgtcacc aggcggccgt
     8821 tcttggcctt cttcgtacgc tgcatggcaa cgtgcgtggt gtttaaccga atgcaggttt
     8881 ctaccaggtc gtctttctgc tttccgccat cggctcgccg gcagaacttg agtacgtccg
     8941 caacgtgtgg acggaacacg cggccgggct tgtctccctt cccttcccgg tatcggttca
     9001 tggattcggt tagatgggaa accgccatca gtaccaggtc gtaatcccac acactggcca
     9061 tgccggccgg ccctgcggaa acctctacgt gcccgtctgg aagctcgtag cggatcacct
     9121 cgccagctcg tcggtcacgc ttcgacagac ggaaaacggc cacgtccatg atgctgcgac
     9181 tatcgcgggt gcccacgtca tagagcatcg gaacgaaaaa atctggttgc tcgtcgccct
     9241 tgggcggctt cctaatcgac ggcgcaccgg ctgccggcgg ttgccgggat tctttgcgga
     9301 ttcgatcagc ggccgcttgc cacgattcac cggggcgtgc ttctgcctcg atgcgttgcc
     9361 gctgggcggc ctgcgcggcc ttcaacttct ccaccaggtc atcacccagc gccgcgccga
     9421 tttgtaccgg gccggatggt ttgcgaccgc tcacgccgat tcctcgggct tgggggttcc
     9481 agtgccattg cagggccggc aggcaaccca gccgcttacg cctggccaac cgcccgttcc
     9541 tccacacatg gggcattcca cggcgtcggt gcctggttgt tcttgatttt ccatgccgcc
     9601 tcctttagcc gctaaaattc atctactcat ttattcattt gctcatttac tctggtagct
     9661 gcgcgatgta ttcagatagc agctcggtaa tggtcttgcc ttggcgtacc gcgtacatct
     9721 tcagcttggt gtgatcctcc gccggcaact gaaagttgac ccgcttcatg gctggcgtgt
     9781 ctgccaggct ggccaacgtt gcagccttgc tgctgcgtgc gctcggacgg ccggcactta
     9841 gcgtgtttgt gcttttgctc attttctctt tacctcatta actcaaatga gttttgattt
     9901 aatttcagcg gccagcgcct ggacctcgcg ggcagcgtcg ccctcgggtt ctgattcaag
     9961 aacggttgtg ccggcggcgg cagtgcctgg gtagctcacg cgctgcgtga tacgggactc
    10021 aagaatgggc agctcgtacc cggccagcgc ctcggcaacc tcaccgccga tgcgcgtgcc
    10081 tttgatcgcc cgcgacacga caaaggccgc ttgtagcctt ccatccgtga cctcaatgcg
    10141 ctgcttaacc agctccacca ggtcggcggt ggcccatatg tcgtaagggc ttggctgcac
    10201 cggaatcagc acgaagtcgg ctgccttgat cgcggacaca gccaagtccg ccgcctgggg
    10261 cgctccgtcg atcactacga agtcgcgccg gccgatggcc ttcacgtcgc ggtcaatcgt
    10321 cgggcggtcg atgccgacaa cggttagcgg ttgatcttcc cgcacggccg cccaatcgcg
    10381 ggcactgccc tggggatcgg aatcgactaa cagaacatcg gccccggcga gttgcagggc
    10441 gcgggctaga tgggttgcga tggtcgtctt gcctgacccg cctttctggt taagtacagc
    10501 gataaccttc atgcgttccc cttgcgtatt tgtttattta ctcatcgcat catatacgca
    10561 gcgaccgcat gacgcaagct gttttactca aatacacatc acctttttag acggcggcgc
    10621 tcggtttctt cagcggccaa gctggccggc caggccgcca gcttggcatc agacaaaccg
    10681 gccaggattt catgcagccg cacggttgag acgtgcgcgg gcggctcgaa cacgtacccg
    10741 gccgcgatca tctccgcctc gatctcttcg gtaatgaaaa acggttcgtc ctggccgtcc
    10801 tggtgcggtt tcatgcttgt tcctcttggc gttcattctc ggcggccgcc agggcgtcgg
    10861 cctcggtcaa tgcgtcctca cggaaggcac cgcgccgcct ggcctcggtg ggcgtcactt
    10921 cctcgctgcg ctcaagtgcg cggtacaggg tcgagcgatg cacgccaagc agtgcagccg
    10981 cctctttcac ggtgcggcct tcctggtcga tcagctcgcg ggcgtgcgcg atctgtgccg
    11041 gggtgagggt agggcggggg ccaaacttca cgcctcgggc cttggcggcc tcgcgcccgc
    11101 tccgggtgcg gtcgatgatt agggaacgct cgaactcggc aatgccggcg aacacggtca
    11161 acaccatgcg gccggccggc gtggtggtgt cggcccacgg ctctgccagg ctacgcaggc
    11221 ccgcgccggc ctcctggatg cgctcggcaa tgtccagtag gtcgcgggtg ctgcgggcca
    11281 ggcggtctag cctggtcact gtcacaacgt cgccagggcg taggtggtca agcatcctgg
    11341 ccagctccgg gcggtcgcgc ctggtgccgg tgatcttctc ggaaaacagc ttggtgcagc
    11401 cggccgcgtg cagttcggcc cgttggttgg tcaagtcctg gtcgtcggtg ctgacgcggg
    11461 catagcccag caggccagcg gcggcgctct tgttcatggc gtaatgtctc cggttctagt
    11521 cgcaagtatt ctactttatg cgactaaaac acgcgacaag aaaacgccag gaaaagggca
    11581 gggcggcagc ctgtcgcgta acttaggact tgtgcgacat gtcgttttca gaagacggct
    11641 gcactgaacg tcagaagccg actgcactat agcagcggag gggttggatc aaagtacttt
    11701 gatcccgagg ggaaccctgt ggttggcatg cacatacaaa tggacgaacg gataaacctt
    11761 ttcacgccct tttaaatatc cgattattct aataaacgct cttttctctt aggtttaccc
    11821 gccaatatat cctgtcaaac actgatagtt taattcccga tctagtaaca tagatgacac
    11881 cgcgcgcgat aatttatcct agtttgcgcg ctatattttg ttttctatcg cgtattaaat
    11941 gtataattgc gggactctaa tcataaaaac ccatctcata aataacgtca tgcattacat
    12001 gttaattatt acatgcttaa cgtaattcaa cagaaattat atgataatca tcgcaagacc
    12061 ggcaacagga ttcaatctta agaaacttta ttgccaaatg tttgaacgat cggggaaatt
    12121 cgagctggtc accaattcac acgtgatggt gatggtgatg gctagcgttc ttgtagccga
    12181 aatctggaat gttggtccag cgctcgcgaa agacgtgcgc ggcgagcttc ggcttgcggt
    12241 cacgagtgaa cacgcccttc ttgtttcctt ggacgcgcat cacgccctga gaggtcgcga
    12301 agtccgcgaa gttccacgct tgctcaccca cgaagttctc aaactcatcg aacacgacgt
    12361 ggttcgcctg gtagtactcg acttgatatt cctcggtgaa catcactgga tcaatgtcgt
    12421 gaaagcccgc aacggtgtct gcgccgtact cagtgatcat gatcggcttt cctgggcaac
    12481 gcttgttcca cgcgtgaaat tcctggcgga gatggacttt ggccgcttcg agatcaccgc
    12541 catcgaagta ccatccgtta tagcgattga gcgcgatgac gtcaatcagt tcggcgactt
    12601 tgtccgtctc cggggtagcc atcacaaaca gcacgatcgt gaccggacgc ttctgtgggt
    12661 cgagttcctt ggtcagctcc accaacggct tgaagtactc gtacgcgccc tcttcctcag
    12721 tcgccgcctc gttggcgatg ctccacatca cgacgcttgg atggttcttg tcacgagaca
    12781 ccagttcacg gagaacgtct tgatggtgct caaacgtccg aatcttctcc caggtactga
    12841 cgcgctcgct gccttcgccg agtcccgtgg tggccatgaa gttgaggtgc acgccaactg
    12901 ccggagtctc gtcgatcacg accagaccct cgcgatccgc aagacgcatc aactcttcag
    12961 agtacggata gtgtgcggtc cggaagctgt tggcgccgat ccatttgagg atattgaaat
    13021 ccatcacatt gctcgcttcg ttaaagccac ggccgttgat aggagtgtcc tcatgtttgc
    13081 caaagccctt gaagtagaac ggtttgttgt tgatgaggaa cttgccgtcg ttgacttcca
    13141 cggtccgcac gccgaacggc tcttcataga catcgatggt cagtccgtcg ttcaccagtt
    13201 ccactttgat ctggtagaga tacgtgttca gtggttccca gaggatgaca ttcggaatct
    13261 ccacgttacc gctcaggccc tcggtgcttg cgaccacttt gccttcctca tccacgaccg
    13321 acactttcac ggtctcggct ttgccttgaa agtccaccgt ataggtcaca gtcccggttg
    13381 ggccattgaa gtcggtcaca accgagatgt cctcgacgta cgtaaacggg gtcgtgtaga
    13441 ttttcaccgg acggtgcagg cctgcatagt tgaagaagtc gaagttcggc ttgttacgaa
    13501 tgacttttcc gaggccctct tcgtggcgct cgctgtacag ccccaccggg agggtgctat
    13561 cgtcgaggat gttgtccacg gcgacggtga cgcgattcat gccatcacgc agcgagttgt
    13621 tgatttccgc ttcgaatggc aggaatccgc ccttgtgctc cacgaccagc tcaccattga
    13681 catagacaat tgctttgtga gttgcagagc cgaagcggag cacgatacgc tgatccttca
    13741 gataggccgg caccgtgaac tcacgttcgt accagacata tccgatatgg ttgcggattt
    13801 ccttggtcac gccaatgtca ttgtaactgc ttgggacggc catactaata gtgtcggtca
    13861 gcttgctttc gtaccacttc tcttccagtc ctttcccgta gtccagcttg aagttccaga
    13921 cgccattgag gtcgaagacg ccacgggtct cggtgttgat cgggtacaga ctagttcgtc
    13981 ggttctgtaa ctatcatcat catcatagac acacgaaata aagtaatcag attatcagtt
    14041 aaagctatgt aatatttaca ccataaccaa tcaattaaaa aatagatcag tttaaagaaa
    14101 gatcaaagct caaaaaaata aaaagagaaa agggtcctaa ccaagaaaat gaaggagaaa
    14161 aactagaaat ttaccctcag atctaccatg
//
