LOCUS       sequence-476980-       14603 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Ribozyme split sfGFP expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14603)
  AUTHORS   Liu Y, Rajput R, Islam MT, Valle ID, Yao T, Agrawal R, Boone BA, 
            Eckert CA, Abraham PE, Chen JG, Tuskan GA, Yang X
  TITLE     A split ribozyme system for in vivo plant RNA imaging and genetic 
            engineering.
  JOURNAL   Plant Biotechnol J. 2025 May;23(5):1640-1649. doi: 
            10.1111/pbi.14612. Epub 2025 Feb 7.
  PUBMED    39919021
REFERENCE   2  (bases 1 to 14603)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/pbi.14612";
            journalName: "Plant Biotechnol J"; date: "2025-05"; volume: "23"; 
            issue: "5"; pages: "1640-1649"
COMMENT     Sequence Label: pXYB034
FEATURES             Location/Qualifiers
     source          1..14603
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(16..361)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     723..745
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     737..754
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     738..754
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1269..1614
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(1769..1797)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     2805..2825
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     terminator      2870..3118
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     primer_bind     complement(3808..3824)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3808..3824)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3821..3843)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3832..3848
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3856..3862,3863..3880,3881..3886))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3901..3922
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        4113..4789
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     CDS             4857..5654
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLL
                     SSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEH
                     QGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDI
                     ALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4914..4933)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5524..5543
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5694..5868
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    5946..5970
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6395..7189
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      7276..7864
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7765..7784
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8018..8035
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8050..8190
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8116..8135)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8276..8298
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8534..8728
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8794..9867)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10296..10925)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    12226..12250
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     terminator      complement(12272..12524)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(12556..12573)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(join(12580..14397,14588..14602))
                     /codon_start=1
                     /product="beta-glucuronidase"
                     /label=GUSPlus(TM)
                     /note="codon-optimized Staphylococcus gusA gene with a 
                     catalase intron to ensure expression in plants but not 
                     bacteria"
                     /translation="MVDLRNRRTSLYPINTETRGVFDLNGVWNFKLDYGKGLEEKWYES
                     KLTDTISMAVPSSYNDIGVTKEIRNHIGYVWYEREFTVPAYLKDQRIVLRFGSATHKAI
                     VYVNGELVVEHKGGFLPFEAEINNSLRDGMNRVTVAVDNILDDSTLPVGLYSERHEEGL
                     GKVIRNKPNFDFFNYAGLHRPVKIYTTPFTYVEDISVVTDFNGPTGTVTYTVDFQGKAE
                     TVKVSVVDEEGKVVASTEGLSGNVEIPNVILWEPLNTYLYQIKVELVNDGLTIDVYEEP
                     FGVRTVEVNDGKFLINNKPFYFKGFGKHEDTPINGRGFNEASNVMDFNILKWIGANSFR
                     TAHYPYSEELMRLADREGLVVIDETPAVGVHLNFMATTGLGEGSERVSTWEKIRTFEHH
                     QDVLRELVSRDKNHPSVVMWSIANEAATEEEGAYEYFKPLVELTKELDPQKRPVTIVLF
                     VMATPETDKVAELIDVIALNRYNGWYFDGGDLEAAKVHLRQEFHAWNKRCPGKPIMITE
                     YGADTVAGFHDIDPVMFTEEYQVEYYQANHVVFDEFENFVGEQAWNFADFATSQGVMRV
                     QGNKKGVFTRDRKPKLAAHVFRERWTNIPDFGYKN"
     intron          14398..14587
                     /label=cat1 intron
                     /note="castor bean catalase intron, modified"
ORIGIN
        1 gtcaagagtc ccccgtgttc tctccaaatg aaatgaactt ccttatatag aggaagggtc
       61 ttgcgaagga tagtgggatt gtgcgtcatc ccttacgtca gtggagatat cacatcaatc
      121 cacttgcttt gaagacgtgg ttggaacgtc ttctttttcc acgatgctcc tcgtgggtgg
      181 gggtccatct ttgggaccac tgtcggcaga ggcatcttca acgatggcct ttcctttatc
      241 gcaatgatgg catttgtagg agccaccttc cttttccact atcttcacaa taaagtgaca
      301 gatagctggg caatggaatc cgaggaggtt tccggatatt accctttgtt gaaaagtctc
      361 aattgccctt tggtcttctg agactgtatc tttgatattt ttggagtaga caagtgtgtc
      421 gtgctccacc atgttgacga agattttctt cttgtcattg agtcgtaaga gactctgtat
      481 gaactgttcg ccagtcttta cggcgagttc tgttaggtcc tctatttgaa tctttgactc
      541 catgaagcta aactgaaggc gggaaacgac aatctgatcc aagctcaagc tgctctagca
      601 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
      661 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt
      721 ttcccagtca cgacgttgta aaacgacggc cagtgccaag cttgcatgcc tgcaggtccc
      781 cagattagcc ttttcaattt cagaaagaat gctaacccac agatggttag agaggcttac
      841 gcagcaggtc tcatcaagac gatctacccg agcaataatc tccaggaaat caaatacctt
      901 cccaagaagg ttaaagatgc agtcaaaaga ttcaggacta actgcatcaa gaacacagag
      961 aaagatatat ttctcaagat cagaagtact attccagtat ggacgattca aggcttgctt
     1021 cacaaaccaa ggcaagtaat agagattgga gtctctaaaa aggtagttcc cactgaatca
     1081 aaggccatgg agtcaaagat tcaaatagag gacctaacag aactcgccgt aaagactggc
     1141 gaacagttca tacagagtct cttacgactc aatgacaaga agaaaatctt cgtcaacatg
     1201 gtggagcacg acacacttgt ctactccaaa aatatcaaag atacagtctc agaagaccaa
     1261 agggcaattg agacttttca acaaagggta atatccggaa acctcctcgg attccattgc
     1321 ccagctatct gtcactttat tgtgaagata gtggaaaagg aaggtggctc ctacaaatgc
     1381 catcattgcg ataaaggaaa ggccatcgtt gaagatgcct ctgccgacag tggtcccaaa
     1441 gatggacccc cacccacgag gagcatcgtg gaaaaagaag acgttccaac cacgtcttca
     1501 aagcaagtgg attgatgtga tatctccact gacgtaaggg atgacgcaca atcccactat
     1561 ccttcgcaag acccttcctc tatataagga agttcatttc atttggagag aacacggggg
     1621 actcaaacat tactcattca caaaaccatc ttaaagcaac tacacaagtc ttgaaatttt
     1681 ctcatatttt ctatttacta tataaacttt taatcaaatc aagattaaag ttaattaaat
     1741 gagcaaagga gaagaacttt tcactggagt tgtcccaatt cttgttgaat tagatggtga
     1801 tgttaatggg cacaaatttt ctgtccgtgg agagggtgaa ggtgatgcta caaacggaaa
     1861 actcaccctt aaatttattt gcactactgg aaaactacct gttccgtggc caacacttgt
     1921 cactactctg acctaaatag caatatttac ctttgggtca aaaagttatc aggcatgcac
     1981 ctggtagcta gtctttaaac caatagattg catcggttta aaaggcaaga ccgtcaaatt
     2041 gcgggaaagg ggtcaacagc cgttcagtac caagtctcag gggaaacttt gagatggcct
     2101 tgcaaagggt atggtaataa gctgacggac atggtcctaa ccacgcagcc aagtcctaag
     2161 tcaacagatc ttctgttgat atggatgcag ttcacagact aaatgtcggt cggggaagat
     2221 gtattcttct cataagatat agtcggacct ctccttaatg ggagctagcg gatgaagtga
     2281 tgcaacactg gagccgctgg gaactaattt gtatgcgaaa gtatattgat tagttttgga
     2341 gtactcgatg gtgttcaatg cttttcccgt tatccggatc acatgaaacg gcatgacttt
     2401 ttcaagagtg ccatgcccga aggttatgta caggaacgca ctatatcttt caaagatgac
     2461 gggacctaca agacgcgtgc tgaagtcaag tttgaaggtg atacccttgt taatcgtatc
     2521 gagttaaagg gtattgattt taaagaagat ggaaacattc ttggacacaa actcgagtac
     2581 aactttaact cacacaatgt atacatcacg gcagacaaac aaaagaatgg aatcaaagct
     2641 aacttcaaaa ttcgccacaa cgttgaagat ggttccgttc aactagcaga ccattatcaa
     2701 caaaatactc caattggcga tggccctgtc cttttaccag acaaccatta cctgtcgaca
     2761 caatctgtcc tttcgaaaga tcccaacgaa aagcgtgacc acatggtcct tcttgagttt
     2821 gtaactgctg ctgggattac acatggcatg gatgagctct acaaataaat atgaagatga
     2881 agatgaaata tttggtgtgt caaataaaaa gcttgtgtgc ttaagtttgt gtttttttct
     2941 tggcttgttg tgttatgaat ttgtggcttt ttctaatatt aaatgaatgt aagatctcat
     3001 tataatgaat aaacaaatgt ttctataatc cattgtgaat gttttgttgg atctcttctg
     3061 cagcatataa ctactgtatg tgctatggta tggactatgg aatatgatta aagataagat
     3121 gggctcatag agtaaaacga ggcgagggac ctataaacct cccttcatca tgctatttca
     3181 tgatctattt tataaaataa agatgtagaa aaaagtaagc gtaataaccg caaaacaaat
     3241 gatttaaaac atggcacata atgaggagat taagttcggt ttacgtttat tttagtacta
     3301 attgtaacgt gagactacgt atcgggaatc gcctaattaa agcattaatg cgaacctgat
     3361 tagattcacc gaccctccta tcgtgtcgac ctttctgttt cttagaattt tttggtagtc
     3421 tatgtactaa taatgtcagc ttcgtattta tttcataagc aatttgcatt tgcaatttgt
     3481 tttttacttt tatttttatt gtattgtgga atgtggactc gtaccaacat gaagttatat
     3541 accaccaaaa aaattacagt tagtcaaaag attcacgagt gagagctact tatgattgtc
     3601 ttttacgtat atgtctaatt gtctatttgc tcaataatct ttgtactttc ttttgtcgtt
     3661 gataaaatca caaagttcca aaagtaatcg aatgatttgc ttttaagaaa agaagagctc
     3721 aataattcaa catatatctg tacacaatta ccgcctttga gtgagctcta gatgccacct
     3781 gacgtctaag aagaattcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     3841 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     3901 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     3961 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     4021 tggctagagc agcttgccaa catggtggag cacgacactc tcgtctactc caagaatatc
     4081 aaagatacag tctcagaaga ccaaagggct attgagactt ttcaacaaag ggtaatatcg
     4141 ggaaacctcc tcggattcca ttgcccagct atctgtcact tcatcaaaag gacagtagaa
     4201 aaggaaggtg gcacctacaa atgccatcat tgcgataaag gaaaggctat cgttcaagat
     4261 gcctctgccg acagtggtcc caaagatgga cccccaccca cgaggagcat cgtggaaaaa
     4321 gaagacgttc caaccacgtc ttcaaagcaa gtggattgat gtgaacatgg tggagcacga
     4381 cactctcgtc tactccaaga atatcaaaga tacagtctca gaagaccaaa gggctattga
     4441 gacttttcaa caaagggtaa tatcgggaaa cctcctcgga ttccattgcc cagctatctg
     4501 tcacttcatc aaaaggacag tagaaaagga aggtggcacc tacaaatgcc atcattgcga
     4561 taaaggaaag gctatcgttc aagatgcctc tgccgacagt ggtcccaaag atggaccccc
     4621 acccacgagg agcatcgtgg aaaaagaaga cgttccaacc acgtcttcaa agcaagtgga
     4681 ttgatgtgat atctccactg acgtaaggga tgacgcacaa tcccactatc cttcgcaaga
     4741 cccttcctct atataaggaa gttcatttca tttggagagg acacgctgaa atcaccagtc
     4801 tctctctaca aatctatctc tctcgagctt tcgcagatcc cggggggcaa tgagatatgg
     4861 ggattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg
     4921 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag
     4981 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactcc
     5041 aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc
     5101 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg
     5161 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc
     5221 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca
     5281 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag
     5341 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg
     5401 gcgaggatct cgtcgtgaca catggcgatg cctgcttgcc gaatatcatg gtggaaaatg
     5461 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca
     5521 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc
     5581 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg
     5641 acgagttctt ctgaagtaga tgccgaccgg atctgtcgat cgacaagctc gagtttctcc
     5701 ataataatgt gtgagtagtt cccagataag ggaattaggg ttcctatagg gtttcgctca
     5761 tgtgttgagc atataagaaa cccttagtat gtatttgtat ttgtaaaata cttctatcaa
     5821 taaaatttct aattcctaaa accaaaatcc agtactaaaa tccagatccc ccgaattaat
     5881 tcggcgttaa ttcagtacat taaaaacgtc cgcaatgtgt tattaagttg tctaagcgtc
     5941 aatttgttta caccacaata tatcctgcca ccagccagcc aacagctccc cgaccggcag
     6001 ctcggcacaa aatcaccact cgatacaggc agcccatcag tccgggacgg cgtcagcggg
     6061 agagccgttg taaggcggca gactttgctc atgttaccga tgctattcgg aagaacggca
     6121 actaagctgc cgggtttgaa acacggatga tctcgcggag ggtagcatgt tgattgtaac
     6181 gatgacagag cgttgctgcc tgtgatcacc gcggtttcaa aatcggctcc gtcgatacta
     6241 tgttatacgc caactttgaa aacaactttg aaaaagctgt tttctggtat ttaaggtttt
     6301 agaatgcaag gaacagtgaa ttggagttcg tcttgttata attagcttct tggggtatct
     6361 ttaaatactg tagaaaagag gaaggaaata ataaatggct aaaatgagaa tatcaccgga
     6421 attgaaaaaa ctgatcgaaa aataccgctg cgtaaaagat acggaaggaa tgtctcctgc
     6481 taaggtatat aagctggtgg gagaaaatga aaacctatat ttaaaaatga cggacagccg
     6541 gtataaaggg accacctatg atgtggaacg ggaaaaggac atgatgctat ggctggaagg
     6601 aaagctgcct gttccaaagg tcctgcactt tgaacggcat gatggctgga gcaatctgct
     6661 catgagtgag gccgatggcg tcctttgctc ggaagagtat gaagatgaac aaagccctga
     6721 aaagattatc gagctgtatg cggagtgcat caggctcttt cactccatcg acatatcgga
     6781 ttgtccctat acgaatagct tagacagccg cttagccgaa ttggattact tactgaataa
     6841 cgatctggcc gatgtggatt gcgaaaactg ggaagaagac actccattta aagatccgcg
     6901 cgagctgtat gattttttaa agacggaaaa gcccgaagag gaacttgtct tttcccacgg
     6961 cgacctggga gacagcaaca tctttgtgaa agatggcaaa gtaagtggct ttattgatct
     7021 tgggagaagc ggcagggcgg acaagtggta tgacattgcc ttctgcgtcc ggtcgatcag
     7081 ggaggatatc ggggaagaac agtatgtcga gctatttttt gacttactgg ggatcaagcc
     7141 tgattgggag aaaataaaat attatatttt actggatgaa ttgttttagt acctagaatg
     7201 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     7261 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     7321 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     7381 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta
     7441 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     7501 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     7561 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     7621 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     7681 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     7741 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     7801 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     7861 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     7921 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     7981 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     8041 ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat
     8101 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg
     8161 ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg
     8221 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
     8281 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gggtgccttg
     8341 atgtgggcgc cggcggtcga gtggcgacgg cgcggcttgt ccgcgccctg gtagattgcc
     8401 tggccgtagg ccagccattt ttgagcggcc agcggccgcg ataggccgac gcgaagcggc
     8461 ggggcgtagg gagcgcagcg accgaagggt aggcgctttt tgcagctctt cggctgtgcg
     8521 ctggccagac agttatgcac aggccaggcg ggttttaaga gttttaataa gttttaaaga
     8581 gttttaggcg gaaaaatcgc cttttttctc ttttatatca gtcacttaca tgtgtgaccg
     8641 gttcccaatg tacggctttg ggttcccaat gtacgggttc cggttcccaa tgtacggctt
     8701 tgggttccca atgtacgtgc tatccacagg aaagagacct tttcgacctt tttcccctgc
     8761 tagggcaatt tgccctagca tctgctccgt acattaggaa ccggcggatg cttcgccctc
     8821 gatcaggttg cggtagcgca tgactaggat cgggccagcc tgccccgcct cctccttcaa
     8881 atcgtactcc ggcaggtcat ttgacccgat cagcttgcgc acggtgaaac agaacttctt
     8941 gaactctccg gcgctgccac tgcgttcgta gatcgtcttg aacaaccatc tggcttctgc
     9001 cttgcctgcg gcgcggcgtg ccaggcggta gagaaaacgg ccgatgccgg gatcgatcaa
     9061 aaagtaatcg gggtgaaccg tcagcacgtc cgggttcttg ccttctgtga tctcgcggta
     9121 catccaatca gctagctcga tctcgatgta ctccggccgc ccggtttcgc tctttacgat
     9181 cttgtagcgg ctaatcaagg cttcaccctc ggataccgtc accaggcggc cgttcttggc
     9241 cttcttcgta cgctgcatgg caacgtgcgt ggtgtttaac cgaatgcagg tttctaccag
     9301 gtcgtctttc tgctttccgc catcggctcg ccggcagaac ttgagtacgt ccgcaacgtg
     9361 tggacggaac acgcggccgg gcttgtctcc cttcccttcc cggtatcggt tcatggattc
     9421 ggttagatgg gaaaccgcca tcagtaccag gtcgtaatcc cacacactgg ccatgccggc
     9481 cggccctgcg gaaacctcta cgtgcccgtc tggaagctcg tagcggatca cctcgccagc
     9541 tcgtcggtca cgcttcgaca gacggaaaac ggccacgtcc atgatgctgc gactatcgcg
     9601 ggtgcccacg tcatagagca tcggaacgaa aaaatctggt tgctcgtcgc ccttgggcgg
     9661 cttcctaatc gacggcgcac cggctgccgg cggttgccgg gattctttgc ggattcgatc
     9721 agcggccgct tgccacgatt caccggggcg tgcttctgcc tcgatgcgtt gccgctgggc
     9781 ggcctgcgcg gccttcaact tctccaccag gtcatcaccc agcgccgcgc cgatttgtac
     9841 cgggccggat ggtttgcgac cgctcacgcc gattcctcgg gcttgggggt tccagtgcca
     9901 ttgcagggcc ggcaggcaac ccagccgctt acgcctggcc aaccgcccgt tcctccacac
     9961 atggggcatt ccacggcgtc ggtgcctggt tgttcttgat tttccatgcc gcctccttta
    10021 gccgctaaaa ttcatctact catttattca tttgctcatt tactctggta gctgcgcgat
    10081 gtattcagat agcagctcgg taatggtctt gccttggcgt accgcgtaca tcttcagctt
    10141 ggtgtgatcc tccgccggca actgaaagtt gacccgcttc atggctggcg tgtctgccag
    10201 gctggccaac gttgcagcct tgctgctgcg tgcgctcgga cggccggcac ttagcgtgtt
    10261 tgtgcttttg ctcattttct ctttacctca ttaactcaaa tgagttttga tttaatttca
    10321 gcggccagcg cctggacctc gcgggcagcg tcgccctcgg gttctgattc aagaacggtt
    10381 gtgccggcgg cggcagtgcc tgggtagctc acgcgctgcg tgatacggga ctcaagaatg
    10441 ggcagctcgt acccggccag cgcctcggca acctcaccgc cgatgcgcgt gcctttgatc
    10501 gcccgcgaca cgacaaaggc cgcttgtagc cttccatccg tgacctcaat gcgctgctta
    10561 accagctcca ccaggtcggc ggtggcccat atgtcgtaag ggcttggctg caccggaatc
    10621 agcacgaagt cggctgcctt gatcgcggac acagccaagt ccgccgcctg gggcgctccg
    10681 tcgatcacta cgaagtcgcg ccggccgatg gccttcacgt cgcggtcaat cgtcgggcgg
    10741 tcgatgccga caacggttag cggttgatct tcccgcacgg ccgcccaatc gcgggcactg
    10801 ccctggggat cggaatcgac taacagaaca tcggccccgg cgagttgcag ggcgcgggct
    10861 agatgggttg cgatggtcgt cttgcctgac ccgcctttct ggttaagtac agcgataacc
    10921 ttcatgcgtt ccccttgcgt atttgtttat ttactcatcg catcatatac gcagcgaccg
    10981 catgacgcaa gctgttttac tcaaatacac atcacctttt tagacggcgg cgctcggttt
    11041 cttcagcggc caagctggcc ggccaggccg ccagcttggc atcagacaaa ccggccagga
    11101 tttcatgcag ccgcacggtt gagacgtgcg cgggcggctc gaacacgtac ccggccgcga
    11161 tcatctccgc ctcgatctct tcggtaatga aaaacggttc gtcctggccg tcctggtgcg
    11221 gtttcatgct tgttcctctt ggcgttcatt ctcggcggcc gccagggcgt cggcctcggt
    11281 caatgcgtcc tcacggaagg caccgcgccg cctggcctcg gtgggcgtca cttcctcgct
    11341 gcgctcaagt gcgcggtaca gggtcgagcg atgcacgcca agcagtgcag ccgcctcttt
    11401 cacggtgcgg ccttcctggt cgatcagctc gcgggcgtgc gcgatctgtg ccggggtgag
    11461 ggtagggcgg gggccaaact tcacgcctcg ggccttggcg gcctcgcgcc cgctccgggt
    11521 gcggtcgatg attagggaac gctcgaactc ggcaatgccg gcgaacacgg tcaacaccat
    11581 gcggccggcc ggcgtggtgg tgtcggccca cggctctgcc aggctacgca ggcccgcgcc
    11641 ggcctcctgg atgcgctcgg caatgtccag taggtcgcgg gtgctgcggg ccaggcggtc
    11701 tagcctggtc actgtcacaa cgtcgccagg gcgtaggtgg tcaagcatcc tggccagctc
    11761 cgggcggtcg cgcctggtgc cggtgatctt ctcggaaaac agcttggtgc agccggccgc
    11821 gtgcagttcg gcccgttggt tggtcaagtc ctggtcgtcg gtgctgacgc gggcatagcc
    11881 cagcaggcca gcggcggcgc tcttgttcat ggcgtaatgt ctccggttct agtcgcaagt
    11941 attctacttt atgcgactaa aacacgcgac aagaaaacgc caggaaaagg gcagggcggc
    12001 agcctgtcgc gtaacttagg acttgtgcga catgtcgttt tcagaagacg gctgcactga
    12061 acgtcagaag ccgactgcac tatagcagcg gaggggttgg atcaaagtac tttgatcccg
    12121 aggggaaccc tgtggttggc atgcacatac aaatggacga acggataaac cttttcacgc
    12181 ccttttaaat atccgattat tctaataaac gctcttttct cttaggttta cccgccaata
    12241 tatcctgtca aacactgata gtttaattcc cgatctagta acatagatga caccgcgcgc
    12301 gataatttat cctagtttgc gcgctatatt ttgttttcta tcgcgtatta aatgtataat
    12361 tgcgggactc taatcataaa aacccatctc ataaataacg tcatgcatta catgttaatt
    12421 attacatgct taacgtaatt caacagaaat tatatgataa tcatcgcaag accggcaaca
    12481 ggattcaatc ttaagaaact ttattgccaa atgtttgaac gatcggggaa attcgagctg
    12541 gtcaccaatt cacacgtgat ggtgatggtg atggctagcg ttcttgtagc cgaaatctgg
    12601 aatgttggtc cagcgctcgc gaaagacgtg cgcggcgagc ttcggcttgc ggtcacgagt
    12661 gaacacgccc ttcttgtttc cttggacgcg catcacgccc tgagaggtcg cgaagtccgc
    12721 gaagttccac gcttgctcac ccacgaagtt ctcaaactca tcgaacacga cgtggttcgc
    12781 ctggtagtac tcgacttgat attcctcggt gaacatcact ggatcaatgt cgtgaaagcc
    12841 cgcaacggtg tctgcgccgt actcagtgat catgatcggc tttcctgggc aacgcttgtt
    12901 ccacgcgtga aattcctggc ggagatggac tttggccgct tcgagatcac cgccatcgaa
    12961 gtaccatccg ttatagcgat tgagcgcgat gacgtcaatc agttcggcga ctttgtccgt
    13021 ctccggggta gccatcacaa acagcacgat cgtgaccgga cgcttctgtg ggtcgagttc
    13081 cttggtcagc tccaccaacg gcttgaagta ctcgtacgcg ccctcttcct cagtcgccgc
    13141 ctcgttggcg atgctccaca tcacgacgct tggatggttc ttgtcacgag acaccagttc
    13201 acggagaacg tcttgatggt gctcaaacgt ccgaatcttc tcccaggtac tgacgcgctc
    13261 gctgccttcg ccgagtcccg tggtggccat gaagttgagg tgcacgccaa ctgccggagt
    13321 ctcgtcgatc acgaccagac cctcgcgatc cgcaagacgc atcaactctt cagagtacgg
    13381 atagtgtgcg gtccggaagc tgttggcgcc gatccatttg aggatattga aatccatcac
    13441 attgctcgct tcgttaaagc cacggccgtt gataggagtg tcctcatgtt tgccaaagcc
    13501 cttgaagtag aacggtttgt tgttgatgag gaacttgccg tcgttgactt ccacggtccg
    13561 cacgccgaac ggctcttcat agacatcgat ggtcagtccg tcgttcacca gttccacttt
    13621 gatctggtag agatacgtgt tcagtggttc ccagaggatg acattcggaa tctccacgtt
    13681 accgctcagg ccctcggtgc ttgcgaccac tttgccttcc tcatccacga ccgacacttt
    13741 cacggtctcg gctttgcctt gaaagtccac cgtataggtc acagtcccgg ttgggccatt
    13801 gaagtcggtc acaaccgaga tgtcctcgac gtacgtaaac ggggtcgtgt agattttcac
    13861 cggacggtgc aggcctgcat agttgaagaa gtcgaagttc ggcttgttac gaatgacttt
    13921 tccgaggccc tcttcgtggc gctcgctgta cagccccacc gggagggtgc tatcgtcgag
    13981 gatgttgtcc acggcgacgg tgacgcgatt catgccatca cgcagcgagt tgttgatttc
    14041 cgcttcgaat ggcaggaatc cgcccttgtg ctccacgacc agctcaccat tgacatagac
    14101 aattgctttg tgagttgcag agccgaagcg gagcacgata cgctgatcct tcagataggc
    14161 cggcaccgtg aactcacgtt cgtaccagac atatccgata tggttgcgga tttccttggt
    14221 cacgccaatg tcattgtaac tgcttgggac ggccatacta atagtgtcgg tcagcttgct
    14281 ttcgtaccac ttctcttcca gtcctttccc gtagtccagc ttgaagttcc agacgccatt
    14341 gaggtcgaag acgccacggg tctcggtgtt gatcgggtac agactagttc gtcggttctg
    14401 taactatcat catcatcata gacacacgaa ataaagtaat cagattatca gttaaagcta
    14461 tgtaatattt acaccataac caatcaatta aaaaatagat cagtttaaag aaagatcaaa
    14521 gctcaaaaaa ataaaaagag aaaagggtcc taaccaagaa aatgaaggag aaaaactaga
    14581 aatttaccct cagatctacc atg
//
