LOCUS       sequence-476982-       14577 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Deactivated ribozyme split sfGFP expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14577)
  AUTHORS   Liu Y, Rajput R, Islam MT, Valle ID, Yao T, Agrawal R, Boone BA, 
            Eckert CA, Abraham PE, Chen JG, Tuskan GA, Yang X
  TITLE     A split ribozyme system for in vivo plant RNA imaging and genetic 
            engineering.
  JOURNAL   Plant Biotechnol J. 2025 May;23(5):1640-1649. doi: 
            10.1111/pbi.14612. Epub 2025 Feb 7.
  PUBMED    39919021
REFERENCE   2  (bases 1 to 14577)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/pbi.14612";
            journalName: "Plant Biotechnol J"; date: "2025-05"; volume: "23"; 
            issue: "5"; pages: "1640-1649"
COMMENT     Sequence Label: pXYB035
FEATURES             Location/Qualifiers
     source          1..14577
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(16..361)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     723..745
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     737..754
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     738..754
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1269..1614
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(1769..1797)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     2779..2799
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     terminator      2844..3092
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     primer_bind     complement(3782..3798)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3782..3798)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3795..3817)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3806..3822
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3830..3836,3837..3854,3855..3860))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3875..3896
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        4087..4763
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     CDS             4831..5628
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLL
                     SSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEH
                     QGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDI
                     ALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4888..4907)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5498..5517
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5668..5842
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    5920..5944
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6369..7163
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      7250..7838
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7739..7758
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7992..8009
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8024..8164
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8090..8109)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8250..8272
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8508..8702
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8768..9841)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10270..10899)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    12200..12224
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     terminator      complement(12246..12498)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(12530..12547)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(join(12554..14371,14562..14576))
                     /codon_start=1
                     /product="beta-glucuronidase"
                     /label=GUSPlus(TM)
                     /note="codon-optimized Staphylococcus gusA gene with a 
                     catalase intron to ensure expression in plants but not 
                     bacteria"
                     /translation="MVDLRNRRTSLYPINTETRGVFDLNGVWNFKLDYGKGLEEKWYES
                     KLTDTISMAVPSSYNDIGVTKEIRNHIGYVWYEREFTVPAYLKDQRIVLRFGSATHKAI
                     VYVNGELVVEHKGGFLPFEAEINNSLRDGMNRVTVAVDNILDDSTLPVGLYSERHEEGL
                     GKVIRNKPNFDFFNYAGLHRPVKIYTTPFTYVEDISVVTDFNGPTGTVTYTVDFQGKAE
                     TVKVSVVDEEGKVVASTEGLSGNVEIPNVILWEPLNTYLYQIKVELVNDGLTIDVYEEP
                     FGVRTVEVNDGKFLINNKPFYFKGFGKHEDTPINGRGFNEASNVMDFNILKWIGANSFR
                     TAHYPYSEELMRLADREGLVVIDETPAVGVHLNFMATTGLGEGSERVSTWEKIRTFEHH
                     QDVLRELVSRDKNHPSVVMWSIANEAATEEEGAYEYFKPLVELTKELDPQKRPVTIVLF
                     VMATPETDKVAELIDVIALNRYNGWYFDGGDLEAAKVHLRQEFHAWNKRCPGKPIMITE
                     YGADTVAGFHDIDPVMFTEEYQVEYYQANHVVFDEFENFVGEQAWNFADFATSQGVMRV
                     QGNKKGVFTRDRKPKLAAHVFRERWTNIPDFGYKN"
     intron          14372..14561
                     /label=cat1 intron
                     /note="castor bean catalase intron, modified"
ORIGIN
        1 gtcaagagtc ccccgtgttc tctccaaatg aaatgaactt ccttatatag aggaagggtc
       61 ttgcgaagga tagtgggatt gtgcgtcatc ccttacgtca gtggagatat cacatcaatc
      121 cacttgcttt gaagacgtgg ttggaacgtc ttctttttcc acgatgctcc tcgtgggtgg
      181 gggtccatct ttgggaccac tgtcggcaga ggcatcttca acgatggcct ttcctttatc
      241 gcaatgatgg catttgtagg agccaccttc cttttccact atcttcacaa taaagtgaca
      301 gatagctggg caatggaatc cgaggaggtt tccggatatt accctttgtt gaaaagtctc
      361 aattgccctt tggtcttctg agactgtatc tttgatattt ttggagtaga caagtgtgtc
      421 gtgctccacc atgttgacga agattttctt cttgtcattg agtcgtaaga gactctgtat
      481 gaactgttcg ccagtcttta cggcgagttc tgttaggtcc tctatttgaa tctttgactc
      541 catgaagcta aactgaaggc gggaaacgac aatctgatcc aagctcaagc tgctctagca
      601 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
      661 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt
      721 ttcccagtca cgacgttgta aaacgacggc cagtgccaag cttgcatgcc tgcaggtccc
      781 cagattagcc ttttcaattt cagaaagaat gctaacccac agatggttag agaggcttac
      841 gcagcaggtc tcatcaagac gatctacccg agcaataatc tccaggaaat caaatacctt
      901 cccaagaagg ttaaagatgc agtcaaaaga ttcaggacta actgcatcaa gaacacagag
      961 aaagatatat ttctcaagat cagaagtact attccagtat ggacgattca aggcttgctt
     1021 cacaaaccaa ggcaagtaat agagattgga gtctctaaaa aggtagttcc cactgaatca
     1081 aaggccatgg agtcaaagat tcaaatagag gacctaacag aactcgccgt aaagactggc
     1141 gaacagttca tacagagtct cttacgactc aatgacaaga agaaaatctt cgtcaacatg
     1201 gtggagcacg acacacttgt ctactccaaa aatatcaaag atacagtctc agaagaccaa
     1261 agggcaattg agacttttca acaaagggta atatccggaa acctcctcgg attccattgc
     1321 ccagctatct gtcactttat tgtgaagata gtggaaaagg aaggtggctc ctacaaatgc
     1381 catcattgcg ataaaggaaa ggccatcgtt gaagatgcct ctgccgacag tggtcccaaa
     1441 gatggacccc cacccacgag gagcatcgtg gaaaaagaag acgttccaac cacgtcttca
     1501 aagcaagtgg attgatgtga tatctccact gacgtaaggg atgacgcaca atcccactat
     1561 ccttcgcaag acccttcctc tatataagga agttcatttc atttggagag aacacggggg
     1621 actcaaacat tactcattca caaaaccatc ttaaagcaac tacacaagtc ttgaaatttt
     1681 ctcatatttt ctatttacta tataaacttt taatcaaatc aagattaaag ttaattaaat
     1741 gagcaaagga gaagaacttt tcactggagt tgtcccaatt cttgttgaat tagatggtga
     1801 tgttaatggg cacaaatttt ctgtccgtgg agagggtgaa ggtgatgcta caaacggaaa
     1861 actcaccctt aaatttattt gcactactgg aaaactacct gttccgtggc caacacttgt
     1921 cactactctg acctaaaagt tatcaggcat gcacctggta gctagtcttt aaaccaatag
     1981 attgcatcgg tttaaaaggc aagaccgtca aattgcggga aaggggtcaa cagccgttca
     2041 gtaccaagtc tcaggggaaa ctttgagatg gccttgcaaa gggtatggta ataagctgac
     2101 ggacatggtc ctaaccacgc agccaagtcc taagtcaaca gatcttctgt tgatatggat
     2161 gcagttcaca aactaaatgt cggtcgggga agatgtattc ttctcataag atatagtcgg
     2221 acctctcctt aatgggagct agcggatgaa gtgatgcaac actggagccg ctgggaacta
     2281 atttgtatgc gaaagtatat tgattagttt tggagtactc gatggtgttc aatgcttttc
     2341 ccgttatccg gatcacatga aacggcatga ctttttcaag agtgccatgc ccgaaggtta
     2401 tgtacaggaa cgcactatat ctttcaaaga tgacgggacc tacaagacgc gtgctgaagt
     2461 caagtttgaa ggtgataccc ttgttaatcg tatcgagtta aagggtattg attttaaaga
     2521 agatggaaac attcttggac acaaactcga gtacaacttt aactcacaca atgtatacat
     2581 cacggcagac aaacaaaaga atggaatcaa agctaacttc aaaattcgcc acaacgttga
     2641 agatggttcc gttcaactag cagaccatta tcaacaaaat actccaattg gcgatggccc
     2701 tgtcctttta ccagacaacc attacctgtc gacacaatct gtcctttcga aagatcccaa
     2761 cgaaaagcgt gaccacatgg tccttcttga gtttgtaact gctgctggga ttacacatgg
     2821 catggatgag ctctacaaat aaatatgaag atgaagatga aatatttggt gtgtcaaata
     2881 aaaagcttgt gtgcttaagt ttgtgttttt ttcttggctt gttgtgttat gaatttgtgg
     2941 ctttttctaa tattaaatga atgtaagatc tcattataat gaataaacaa atgtttctat
     3001 aatccattgt gaatgttttg ttggatctct tctgcagcat ataactactg tatgtgctat
     3061 ggtatggact atggaatatg attaaagata agatgggctc atagagtaaa acgaggcgag
     3121 ggacctataa acctcccttc atcatgctat ttcatgatct attttataaa ataaagatgt
     3181 agaaaaaagt aagcgtaata accgcaaaac aaatgattta aaacatggca cataatgagg
     3241 agattaagtt cggtttacgt ttattttagt actaattgta acgtgagact acgtatcggg
     3301 aatcgcctaa ttaaagcatt aatgcgaacc tgattagatt caccgaccct cctatcgtgt
     3361 cgacctttct gtttcttaga attttttggt agtctatgta ctaataatgt cagcttcgta
     3421 tttatttcat aagcaatttg catttgcaat ttgtttttta cttttatttt tattgtattg
     3481 tggaatgtgg actcgtacca acatgaagtt atataccacc aaaaaaatta cagttagtca
     3541 aaagattcac gagtgagagc tacttatgat tgtcttttac gtatatgtct aattgtctat
     3601 ttgctcaata atctttgtac tttcttttgt cgttgataaa atcacaaagt tccaaaagta
     3661 atcgaatgat ttgcttttaa gaaaagaaga gctcaataat tcaacatata tctgtacaca
     3721 attaccgcct ttgagtgagc tctagatgcc acctgacgtc taagaagaat tcgtaatcat
     3781 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     3841 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     3901 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     3961 tcggccaacg cgcggggaga ggcggtttgc gtattggcta gagcagcttg ccaacatggt
     4021 ggagcacgac actctcgtct actccaagaa tatcaaagat acagtctcag aagaccaaag
     4081 ggctattgag acttttcaac aaagggtaat atcgggaaac ctcctcggat tccattgccc
     4141 agctatctgt cacttcatca aaaggacagt agaaaaggaa ggtggcacct acaaatgcca
     4201 tcattgcgat aaaggaaagg ctatcgttca agatgcctct gccgacagtg gtcccaaaga
     4261 tggaccccca cccacgagga gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa
     4321 gcaagtggat tgatgtgaac atggtggagc acgacactct cgtctactcc aagaatatca
     4381 aagatacagt ctcagaagac caaagggcta ttgagacttt tcaacaaagg gtaatatcgg
     4441 gaaacctcct cggattccat tgcccagcta tctgtcactt catcaaaagg acagtagaaa
     4501 aggaaggtgg cacctacaaa tgccatcatt gcgataaagg aaaggctatc gttcaagatg
     4561 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
     4621 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
     4681 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
     4741 ttcatttgga gaggacacgc tgaaatcacc agtctctctc tacaaatcta tctctctcga
     4801 gctttcgcag atcccggggg gcaatgagat atggggattg aacaagatgg attgcacgca
     4861 ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc
     4921 ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc
     4981 aagaccgacc tgtccggtgc cctgaatgaa ctccaggacg aggcagcgcg gctatcgtgg
     5041 ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg
     5101 gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct
     5161 gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct
     5221 acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa
     5281 gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa
     5341 ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt gacacatggc
     5401 gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt
     5461 ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct
     5521 gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc
     5581 gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgaag tagatgccga
     5641 ccggatctgt cgatcgacaa gctcgagttt ctccataata atgtgtgagt agttcccaga
     5701 taagggaatt agggttccta tagggtttcg ctcatgtgtt gagcatataa gaaaccctta
     5761 gtatgtattt gtatttgtaa aatacttcta tcaataaaat ttctaattcc taaaaccaaa
     5821 atccagtact aaaatccaga tcccccgaat taattcggcg ttaattcagt acattaaaaa
     5881 cgtccgcaat gtgttattaa gttgtctaag cgtcaatttg tttacaccac aatatatcct
     5941 gccaccagcc agccaacagc tccccgaccg gcagctcggc acaaaatcac cactcgatac
     6001 aggcagccca tcagtccggg acggcgtcag cgggagagcc gttgtaaggc ggcagacttt
     6061 gctcatgtta ccgatgctat tcggaagaac ggcaactaag ctgccgggtt tgaaacacgg
     6121 atgatctcgc ggagggtagc atgttgattg taacgatgac agagcgttgc tgcctgtgat
     6181 caccgcggtt tcaaaatcgg ctccgtcgat actatgttat acgccaactt tgaaaacaac
     6241 tttgaaaaag ctgttttctg gtatttaagg ttttagaatg caaggaacag tgaattggag
     6301 ttcgtcttgt tataattagc ttcttggggt atctttaaat actgtagaaa agaggaagga
     6361 aataataaat ggctaaaatg agaatatcac cggaattgaa aaaactgatc gaaaaatacc
     6421 gctgcgtaaa agatacggaa ggaatgtctc ctgctaaggt atataagctg gtgggagaaa
     6481 atgaaaacct atatttaaaa atgacggaca gccggtataa agggaccacc tatgatgtgg
     6541 aacgggaaaa ggacatgatg ctatggctgg aaggaaagct gcctgttcca aaggtcctgc
     6601 actttgaacg gcatgatggc tggagcaatc tgctcatgag tgaggccgat ggcgtccttt
     6661 gctcggaaga gtatgaagat gaacaaagcc ctgaaaagat tatcgagctg tatgcggagt
     6721 gcatcaggct ctttcactcc atcgacatat cggattgtcc ctatacgaat agcttagaca
     6781 gccgcttagc cgaattggat tacttactga ataacgatct ggccgatgtg gattgcgaaa
     6841 actgggaaga agacactcca tttaaagatc cgcgcgagct gtatgatttt ttaaagacgg
     6901 aaaagcccga agaggaactt gtcttttccc acggcgacct gggagacagc aacatctttg
     6961 tgaaagatgg caaagtaagt ggctttattg atcttgggag aagcggcagg gcggacaagt
     7021 ggtatgacat tgccttctgc gtccggtcga tcagggagga tatcggggaa gaacagtatg
     7081 tcgagctatt ttttgactta ctggggatca agcctgattg ggagaaaata aaatattata
     7141 ttttactgga tgaattgttt tagtacctag aatgcatgac caaaatccct taacgtgagt
     7201 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     7261 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     7321 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     7381 agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     7441 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     7501 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     7561 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     7621 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     7681 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     7741 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     7801 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     7861 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     7921 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     7981 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc
     8041 tccttacgca tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct
     8101 ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc
     8161 tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc
     8221 atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc
     8281 gtcatcaccg aaacgcgcga ggcagggtgc cttgatgtgg gcgccggcgg tcgagtggcg
     8341 acggcgcggc ttgtccgcgc cctggtagat tgcctggccg taggccagcc atttttgagc
     8401 ggccagcggc cgcgataggc cgacgcgaag cggcggggcg tagggagcgc agcgaccgaa
     8461 gggtaggcgc tttttgcagc tcttcggctg tgcgctggcc agacagttat gcacaggcca
     8521 ggcgggtttt aagagtttta ataagtttta aagagtttta ggcggaaaaa tcgccttttt
     8581 tctcttttat atcagtcact tacatgtgtg accggttccc aatgtacggc tttgggttcc
     8641 caatgtacgg gttccggttc ccaatgtacg gctttgggtt cccaatgtac gtgctatcca
     8701 caggaaagag accttttcga cctttttccc ctgctagggc aatttgccct agcatctgct
     8761 ccgtacatta ggaaccggcg gatgcttcgc cctcgatcag gttgcggtag cgcatgacta
     8821 ggatcgggcc agcctgcccc gcctcctcct tcaaatcgta ctccggcagg tcatttgacc
     8881 cgatcagctt gcgcacggtg aaacagaact tcttgaactc tccggcgctg ccactgcgtt
     8941 cgtagatcgt cttgaacaac catctggctt ctgccttgcc tgcggcgcgg cgtgccaggc
     9001 ggtagagaaa acggccgatg ccgggatcga tcaaaaagta atcggggtga accgtcagca
     9061 cgtccgggtt cttgccttct gtgatctcgc ggtacatcca atcagctagc tcgatctcga
     9121 tgtactccgg ccgcccggtt tcgctcttta cgatcttgta gcggctaatc aaggcttcac
     9181 cctcggatac cgtcaccagg cggccgttct tggccttctt cgtacgctgc atggcaacgt
     9241 gcgtggtgtt taaccgaatg caggtttcta ccaggtcgtc tttctgcttt ccgccatcgg
     9301 ctcgccggca gaacttgagt acgtccgcaa cgtgtggacg gaacacgcgg ccgggcttgt
     9361 ctcccttccc ttcccggtat cggttcatgg attcggttag atgggaaacc gccatcagta
     9421 ccaggtcgta atcccacaca ctggccatgc cggccggccc tgcggaaacc tctacgtgcc
     9481 cgtctggaag ctcgtagcgg atcacctcgc cagctcgtcg gtcacgcttc gacagacgga
     9541 aaacggccac gtccatgatg ctgcgactat cgcgggtgcc cacgtcatag agcatcggaa
     9601 cgaaaaaatc tggttgctcg tcgcccttgg gcggcttcct aatcgacggc gcaccggctg
     9661 ccggcggttg ccgggattct ttgcggattc gatcagcggc cgcttgccac gattcaccgg
     9721 ggcgtgcttc tgcctcgatg cgttgccgct gggcggcctg cgcggccttc aacttctcca
     9781 ccaggtcatc acccagcgcc gcgccgattt gtaccgggcc ggatggtttg cgaccgctca
     9841 cgccgattcc tcgggcttgg gggttccagt gccattgcag ggccggcagg caacccagcc
     9901 gcttacgcct ggccaaccgc ccgttcctcc acacatgggg cattccacgg cgtcggtgcc
     9961 tggttgttct tgattttcca tgccgcctcc tttagccgct aaaattcatc tactcattta
    10021 ttcatttgct catttactct ggtagctgcg cgatgtattc agatagcagc tcggtaatgg
    10081 tcttgccttg gcgtaccgcg tacatcttca gcttggtgtg atcctccgcc ggcaactgaa
    10141 agttgacccg cttcatggct ggcgtgtctg ccaggctggc caacgttgca gccttgctgc
    10201 tgcgtgcgct cggacggccg gcacttagcg tgtttgtgct tttgctcatt ttctctttac
    10261 ctcattaact caaatgagtt ttgatttaat ttcagcggcc agcgcctgga cctcgcgggc
    10321 agcgtcgccc tcgggttctg attcaagaac ggttgtgccg gcggcggcag tgcctgggta
    10381 gctcacgcgc tgcgtgatac gggactcaag aatgggcagc tcgtacccgg ccagcgcctc
    10441 ggcaacctca ccgccgatgc gcgtgccttt gatcgcccgc gacacgacaa aggccgcttg
    10501 tagccttcca tccgtgacct caatgcgctg cttaaccagc tccaccaggt cggcggtggc
    10561 ccatatgtcg taagggcttg gctgcaccgg aatcagcacg aagtcggctg ccttgatcgc
    10621 ggacacagcc aagtccgccg cctggggcgc tccgtcgatc actacgaagt cgcgccggcc
    10681 gatggccttc acgtcgcggt caatcgtcgg gcggtcgatg ccgacaacgg ttagcggttg
    10741 atcttcccgc acggccgccc aatcgcgggc actgccctgg ggatcggaat cgactaacag
    10801 aacatcggcc ccggcgagtt gcagggcgcg ggctagatgg gttgcgatgg tcgtcttgcc
    10861 tgacccgcct ttctggttaa gtacagcgat aaccttcatg cgttcccctt gcgtatttgt
    10921 ttatttactc atcgcatcat atacgcagcg accgcatgac gcaagctgtt ttactcaaat
    10981 acacatcacc tttttagacg gcggcgctcg gtttcttcag cggccaagct ggccggccag
    11041 gccgccagct tggcatcaga caaaccggcc aggatttcat gcagccgcac ggttgagacg
    11101 tgcgcgggcg gctcgaacac gtacccggcc gcgatcatct ccgcctcgat ctcttcggta
    11161 atgaaaaacg gttcgtcctg gccgtcctgg tgcggtttca tgcttgttcc tcttggcgtt
    11221 cattctcggc ggccgccagg gcgtcggcct cggtcaatgc gtcctcacgg aaggcaccgc
    11281 gccgcctggc ctcggtgggc gtcacttcct cgctgcgctc aagtgcgcgg tacagggtcg
    11341 agcgatgcac gccaagcagt gcagccgcct ctttcacggt gcggccttcc tggtcgatca
    11401 gctcgcgggc gtgcgcgatc tgtgccgggg tgagggtagg gcgggggcca aacttcacgc
    11461 ctcgggcctt ggcggcctcg cgcccgctcc gggtgcggtc gatgattagg gaacgctcga
    11521 actcggcaat gccggcgaac acggtcaaca ccatgcggcc ggccggcgtg gtggtgtcgg
    11581 cccacggctc tgccaggcta cgcaggcccg cgccggcctc ctggatgcgc tcggcaatgt
    11641 ccagtaggtc gcgggtgctg cgggccaggc ggtctagcct ggtcactgtc acaacgtcgc
    11701 cagggcgtag gtggtcaagc atcctggcca gctccgggcg gtcgcgcctg gtgccggtga
    11761 tcttctcgga aaacagcttg gtgcagccgg ccgcgtgcag ttcggcccgt tggttggtca
    11821 agtcctggtc gtcggtgctg acgcgggcat agcccagcag gccagcggcg gcgctcttgt
    11881 tcatggcgta atgtctccgg ttctagtcgc aagtattcta ctttatgcga ctaaaacacg
    11941 cgacaagaaa acgccaggaa aagggcaggg cggcagcctg tcgcgtaact taggacttgt
    12001 gcgacatgtc gttttcagaa gacggctgca ctgaacgtca gaagccgact gcactatagc
    12061 agcggagggg ttggatcaaa gtactttgat cccgagggga accctgtggt tggcatgcac
    12121 atacaaatgg acgaacggat aaaccttttc acgccctttt aaatatccga ttattctaat
    12181 aaacgctctt ttctcttagg tttacccgcc aatatatcct gtcaaacact gatagtttaa
    12241 ttcccgatct agtaacatag atgacaccgc gcgcgataat ttatcctagt ttgcgcgcta
    12301 tattttgttt tctatcgcgt attaaatgta taattgcggg actctaatca taaaaaccca
    12361 tctcataaat aacgtcatgc attacatgtt aattattaca tgcttaacgt aattcaacag
    12421 aaattatatg ataatcatcg caagaccggc aacaggattc aatcttaaga aactttattg
    12481 ccaaatgttt gaacgatcgg ggaaattcga gctggtcacc aattcacacg tgatggtgat
    12541 ggtgatggct agcgttcttg tagccgaaat ctggaatgtt ggtccagcgc tcgcgaaaga
    12601 cgtgcgcggc gagcttcggc ttgcggtcac gagtgaacac gcccttcttg tttccttgga
    12661 cgcgcatcac gccctgagag gtcgcgaagt ccgcgaagtt ccacgcttgc tcacccacga
    12721 agttctcaaa ctcatcgaac acgacgtggt tcgcctggta gtactcgact tgatattcct
    12781 cggtgaacat cactggatca atgtcgtgaa agcccgcaac ggtgtctgcg ccgtactcag
    12841 tgatcatgat cggctttcct gggcaacgct tgttccacgc gtgaaattcc tggcggagat
    12901 ggactttggc cgcttcgaga tcaccgccat cgaagtacca tccgttatag cgattgagcg
    12961 cgatgacgtc aatcagttcg gcgactttgt ccgtctccgg ggtagccatc acaaacagca
    13021 cgatcgtgac cggacgcttc tgtgggtcga gttccttggt cagctccacc aacggcttga
    13081 agtactcgta cgcgccctct tcctcagtcg ccgcctcgtt ggcgatgctc cacatcacga
    13141 cgcttggatg gttcttgtca cgagacacca gttcacggag aacgtcttga tggtgctcaa
    13201 acgtccgaat cttctcccag gtactgacgc gctcgctgcc ttcgccgagt cccgtggtgg
    13261 ccatgaagtt gaggtgcacg ccaactgccg gagtctcgtc gatcacgacc agaccctcgc
    13321 gatccgcaag acgcatcaac tcttcagagt acggatagtg tgcggtccgg aagctgttgg
    13381 cgccgatcca tttgaggata ttgaaatcca tcacattgct cgcttcgtta aagccacggc
    13441 cgttgatagg agtgtcctca tgtttgccaa agcccttgaa gtagaacggt ttgttgttga
    13501 tgaggaactt gccgtcgttg acttccacgg tccgcacgcc gaacggctct tcatagacat
    13561 cgatggtcag tccgtcgttc accagttcca ctttgatctg gtagagatac gtgttcagtg
    13621 gttcccagag gatgacattc ggaatctcca cgttaccgct caggccctcg gtgcttgcga
    13681 ccactttgcc ttcctcatcc acgaccgaca ctttcacggt ctcggctttg ccttgaaagt
    13741 ccaccgtata ggtcacagtc ccggttgggc cattgaagtc ggtcacaacc gagatgtcct
    13801 cgacgtacgt aaacggggtc gtgtagattt tcaccggacg gtgcaggcct gcatagttga
    13861 agaagtcgaa gttcggcttg ttacgaatga cttttccgag gccctcttcg tggcgctcgc
    13921 tgtacagccc caccgggagg gtgctatcgt cgaggatgtt gtccacggcg acggtgacgc
    13981 gattcatgcc atcacgcagc gagttgttga tttccgcttc gaatggcagg aatccgccct
    14041 tgtgctccac gaccagctca ccattgacat agacaattgc tttgtgagtt gcagagccga
    14101 agcggagcac gatacgctga tccttcagat aggccggcac cgtgaactca cgttcgtacc
    14161 agacatatcc gatatggttg cggatttcct tggtcacgcc aatgtcattg taactgcttg
    14221 ggacggccat actaatagtg tcggtcagct tgctttcgta ccacttctct tccagtcctt
    14281 tcccgtagtc cagcttgaag ttccagacgc cattgaggtc gaagacgcca cgggtctcgg
    14341 tgttgatcgg gtacagacta gttcgtcggt tctgtaacta tcatcatcat catagacaca
    14401 cgaaataaag taatcagatt atcagttaaa gctatgtaat atttacacca taaccaatca
    14461 attaaaaaat agatcagttt aaagaaagat caaagctcaa aaaaataaaa agagaaaagg
    14521 gtcctaacca agaaaatgaa ggagaaaaac tagaaattta ccctcagatc taccatg
//
