LOCUS       sequence-47699-e         820 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Young CL, Raden DL, Caplan JL, Czymmek KJ, Robinson AS
  TITLE     Cassette series designed for live-cell imaging of proteins and 
            high-resolution techniques in yeast.
  JOURNAL   Yeast. 2012 Mar;29(3-4):119-36. doi: 10.1002/yea.2895. Epub 2012 Apr
            4.
  PUBMED    22473760
REFERENCE   2  (bases 1 to 820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast."; 
            date: "2012-03"; volume: "29(3-4):119-36. doi"; pages: " 
            10.1002/yea.2895. Epub 2012 Apr 4"
COMMENT     Sequence Label: pCY 3080-04
FEATURES             Location/Qualifiers
     source          1..820
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(117..791)
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
ORIGIN
        1 atcatcgatg aattcgagct cgggtatttt ctccttacgc atctgtgcgg tatttcacac
       61 cgcatagatc ggcaagtgca caaacaatac ttaaataaat actactcagt aataacctat
      121 ttcttagcat ttttgacgaa atttgctatt ttgttagagt cttttacacc atttgtctcc
      181 acacctccgc ttacatcaac accaataacg ccatttaatc taagcgcatc accaacattt
      241 tctggcgtca gtccaccagc taacataaaa tgtaagcttt cggggctctc ttgccttcca
      301 acccagtcag aaatcgagtt ccaatccaaa agttcacctg tcccacctgc ttctgaatca
      361 aacaagggaa taaacgaatg aggtttctgt gaagctgcac tgagtagtat gttgcagtct
      421 tttggaaata cgagtctttt aataactggc aaaccgagga actcttggta ttcttgccac
      481 gactcatctc catgcagttg gacgatatca atgccgtaat cattgaccag agccaaaaca
      541 tcctccttag gttgattacg aaacacgcca accaagtatt tcggagtgcc tgaactattt
      601 ttatatgctt ttacaagact tgaaattttc cttgcaataa ccgggtcaat tgttctcttt
      661 ctattgggca cacatataat acccagcaag tcagcatcgg aatctagagc acattctgcg
      721 gcctctgtgc tctgcaagcc gcaaactttc accaatggac cagaactacc tgtgaaatta
      781 ataacagaca tactccaagc tgcctttgtg tgcttaatca
//
