LOCUS       sequence-477003-       10635 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses of GFP-tagged mTet1-delCys in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10635)
  AUTHORS   Arroyo M, Hastert FD, Zhadan A, Schelter F, Zimbelmann S, Rausch C, 
            Ludwig AK, Carell T, Cardoso MC
  TITLE     Isoform-specific and ubiquitination dependent recruitment of Tet1 to
            replicating heterochromatin modulates methylcytosine oxidation.
  JOURNAL   Nat Commun. 2022 Sep 2;13(1):5173. doi: 10.1038/s41467-022-32799-8.
  PUBMED    36056023
REFERENCE   2  (bases 1 to 10635)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-09-2"; volume: "13(1):5173. doi"; pages: " 
            10.1038/s41467-022-32799-8"
COMMENT     Sequence Label: pGFP-mTet1-delCys  pc3903
FEATURES             Location/Qualifiers
     source          1..10635
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(1812..1832)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(join(1839..2549,2550..2552,2553..2555))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(1839..2549,2550..2552,2553..2555))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1881..1902)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     2230..2249
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2489..2510
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      2552..2561
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(2606..2625)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     intron          2633..3649
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     complement(2687..2709)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     promoter        complement(3650..3926)
                     /label=chicken beta-actin promoter
     enhancer        3928..4231
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        complement(4300..4318)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4325..4342)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4325..4341)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4334..4356)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4482..4937
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4569..4588)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4779..4800
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5004..5023)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5123..5145
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5183..5201)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5269..5373
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5374..5442,5443..6234)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5592..5611)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6405..6993
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6894..6913
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7147..7164
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      7270..7405
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    7453..7574
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7464..7483)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7518..7537
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(7732..8130)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     regulatory      8127..8136
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    8141..8603
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(8301..8320)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     8530..8547
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
ORIGIN
        1 ggcagagggt gagcttcctg attgacactc accatgggct gtccctgaga attatctgcc
       61 ggctctgcaa atgtctgccc accggattct gtcttgaatt ggcatgttgg cagagaatct
      121 gatggttcaa ccttcacttt ctcctttgcc aattcagggt ttcttgtgaa ttttatctga
      181 ttgataggag gaaataactc attatgttgt gagggtgttt tactctgagt cacgggcttt
      241 aatatttggt taaagacagg aatatttctc aacagaacag gaaaactacg tggactagac
      301 tggccaaacc tttgaaactt agatgacatc caaatgtcat gcattttact atactctata
      361 gctagctgct cttgcttctt ctgatcattt tcttgagagc ttcgggctgg gggtttcttt
      421 ttccgcttgt ttgcgtgtgg ggtcgctctt gcctttttct gggccttctt tggctttgct
      481 gatggtacac tgggtttagt ttgtgtacta gagggtggct tctgatgtac catgcgtttc
      541 tcctgactgt gtttttgctt tgtgttcttt gcaacaaggc tttctggtgt actttcagca
      601 ttcctgacct cggcgttgat gtgattaatc gtggatgcaa gttgggtcaa ttgtgctgcg
      661 acatcttctt caatttgact gtagggtgca tcctggcatg atgggtcatg ggaactcaaa
      721 ttctgatcgc agcttagtac ctgactgcag ttttcaagat tgtgactttc tatgggcaat
      781 ggatgtgaat tctcaaaacc atcaaaggga gctctcccac cagcagcgcc tttcttctca
      841 gctgtaggtg aacttggata tccttgtgat tttataagtg cttggttagt agcgaaacta
      901 tcaggtgact tgaatgttct gttctggcca ttaaagacaa cagtgtcatg gtgaaggccc
      961 tttccttgaa cgtttgggtc ttggaggtct tttctcaccg aatgaaggat agccttgcaa
     1021 ctgttaagca gactagccgt tttctgatgc gcttcttcat ccttctccgg cttggatggg
     1081 gaggagctct ctgagggcgc ttctgagagc tgagtcaggg cttctatggc taccacttgt
     1141 tccagcgtta atctcctgtc cttcattagg gatagaagac ttggctgaac cggagagcca
     1201 tctttgggtt ctttgttggg taccttctca ggagggtgag gtcgactccc agcgctgtgt
     1261 gtattagggt gattattaaa gatggtgggt tctgcaccag acctgcagtt caagccaggc
     1321 ttctggaaga gcagggtgtt ccttggcccc ttgagatgat cacactcccc cccggaggtg
     1381 gtgacactca tggcatcctt gcagtttgag ggatcagtca gcaatttaga ggaattattt
     1441 cccaacgcct tgctaaattc ctcatgattc aatttgttga gcggaaggtg tgtgtcagtg
     1501 ggtaaacagt gtacattttt catgccgttc cttatggtct gggcaaatgt cggagaaggg
     1561 ttggtttgct gcttgtcatc ttcagagggc tgggggtttt ccaccccagt taaattggca
     1621 tcacagcttc ctttgacttg accctctgct aagtgtgatt tcttgtttgg cgcccacttc
     1681 tccacctcaa ttcccgtcat gccgcctgca ttcttcctta cattttcaag gggatgtgtg
     1741 atcaaatcca acctttgctc ttcttcccca tgaccacgtc tactgcgaga catgcgatcg
     1801 cctcgagatc ttccttggaa gtataggttt tctccggact tgtacagctc gtccatgccg
     1861 agagtgatcc cggcggcggt cacgaactcc agcaggacca tgtgatcgcg cttctcgttg
     1921 gggtctttgc tcagggcgga ctgggtgctc aggtagtggt tgtcgggcag cagcacgggg
     1981 ccgtcgccga tgggggtgtt ctgctggtag tggtcggcga gctgcacgct gccgtcctcg
     2041 atgttgtggc ggatcttgaa gttcaccttg atgccgttct tctgcttgtc ggccatgata
     2101 tagacgttgt ggctgttgta gttgtactcc agcttgtgcc ccaggatgtt gccgtcctcc
     2161 ttgaagtcga tgcccttcag ctcgatgcgg ttcaccaggg tgtcgccctc gaacttcacc
     2221 tcggcgcggg tcttgtagtt gccgtcgtcc ttgaagaaga tggtgcgctc ctggacgtag
     2281 ccttcgggca tggcggactt gaagaagtcg tgctgcttca tgtggtcggg gtagcggctg
     2341 aagcactgca cgccgtaggt cagggtggtc acgagggtgg gccagggcac gggcagcttg
     2401 ccggtggtgc agatgaactt cagggtcagc ttgccgtagg tggcatcgcc ctcgccctcg
     2461 ccggacacgc tgaacttgtg gccgtttacg tcgccgtcca gctcgaccag gatgggcacc
     2521 accccggtga acagctcctc gcccttgctc accatggtgg cgaccggccg gtggatccga
     2581 attctttgcc aaaatgatga gacagcacaa taaccagcac gttgcccagg agctgtagga
     2641 aaaagaagaa ggcatgaaca tggttagcag aggctctaga gccgccggtc acacgccaga
     2701 agccgaaccc cgccctgccc cgtccccccc gaaggcagcc gtccccccgc ggacagcccc
     2761 gaggctggag agggagaagg ggacggcggc gcggcgacgc acgaaggccc tccccgccca
     2821 tttccttcct gccggcgccg caccgcttcg ccccgcgccc gctagagggg gtgcggcggc
     2881 gcctcccaga tttcggctcc gcacagattt gggacaaagg aagtccctgc gccctctcgc
     2941 acgattacca taaaaggcaa tggctgcggc tcgccgcgcc tcgacagccg ccggcgctcc
     3001 gggggccgcc gcgcccctcc cccgagccct ccccggcccg aggcggcccc gccccgcccg
     3061 gcacccccac ctgccgccac cccccgcccg gcacggcgag ccccgcgcca cgccccgtac
     3121 ggagccccgc acccgaagcc gggccgtgct cagcaactcg gggagggggg tgcagggggg
     3181 gttgcagccc gaccgacgcg cccacacccc ctgctcaccc ccccacgcac acaccccgca
     3241 cgcagccttt gttcccctcg cagccccccc cgcaccgcgg ggcaccgccc ccggccgcgc
     3301 tcccctcgcg cacactgcgg agcgcacaaa gccccgcgcc gcgcccgcag cgctcacagc
     3361 cgccgggcag cgcggagccg cacgcggcgc tccccacgca cacacacacg cacgcacccc
     3421 ccgagccgct ccccccgcac aaagggccct cccggagccc ctcaaggctt tcacgcagcc
     3481 acagaaaaga aacaagccgt cattaaacca agcgctaatt acagcccgga ggagaagggc
     3541 cgtcccgccc gctcacctgt gggagtaacg cggtcagtca gagccggggc gggcggcgcg
     3601 aggcggcggc ggagcggggc acggggcgaa ggcagcgcgc agcgactccc gcccgccgcg
     3661 cgcttcgctt tttatagggc cgccgccgcc gccgcctcgc cataaaagga aactttcgga
     3721 gcgcgccgct ctgattggct gccgccgcac ctctccgcct cgccccgccc cgcccctcgc
     3781 cccgccccgc cccgcctggc gcgcgccccc cccccccccc gcccccatcg ctgcacaaaa
     3841 taattaaaaa ataaataaat acaaaattgg gggtggggag gggggggaga tggggagagt
     3901 gaagcagaac gtggggctca cctcgaccat ggtaatagcg atgactaata cgtagatgta
     3961 ctgccaagta ggaaagtccc ataaggtcat gtactgggca taatgccagg cgggccattt
     4021 accgtcattg acgtcaatag ggggcgtact tggcatatga tacacttgat gtactgccaa
     4081 gtgggcagtt taccgtaaat actccaccca ttgacgtcaa tggaaagtcc ctattggcgt
     4141 tactatggga acatacgtca ttattgacgt caatgggcgg gggtcgttgg gcggtcagcc
     4201 aggcgggcca tttaccgtaa gttatgtaac gcggaactcc atatatgggc tatgaactaa
     4261 tgaccccgta attgattact attaataact aggacgcgtc ctatagtgag tcgtattaca
     4321 attcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
     4381 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
     4441 atcgcccttc ccaacagttg cgcagcctga atggcgaatg gacgcgccct gtagcggcgc
     4501 attgagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     4561 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     4621 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     4681 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     4741 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     4801 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
     4861 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
     4921 attaacgctt acaatttcct gatgcggtat tttctcctta cgcatctgtg cggtatttca
     4981 caccgcatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc
     5041 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct
     5101 tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca
     5161 ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
     5221 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
     5281 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     5341 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     5401 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     5461 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     5521 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     5581 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     5641 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     5701 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     5761 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     5821 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     5881 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     5941 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     6001 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     6061 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     6121 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     6181 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     6241 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     6301 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     6361 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     6421 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     6481 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     6541 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     6601 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     6661 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     6721 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     6781 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     6841 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     6901 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     6961 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     7021 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     7081 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     7141 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
     7201 cccgcgcgtt ggccgattca ttaatgcagc aattagtcag caaccatagt cccgccccta
     7261 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     7321 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
     7381 tagtgaagag gcttttttgg aggcctaggc ttttgcaaaa agctcctcga ggtcgacggt
     7441 atacagacat gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa
     7501 aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct
     7561 gcaataaaca agttggggtg ggcgaagaac tccagcatga gatccccgcg ctggaggatc
     7621 atccagccgg cgtcccggaa aacgattccg aagcccaacc tttcatagaa ggcggcggtg
     7681 gaatcgaaat ctcgtagcac gtgtcagtcc tgctcctcgg ccacgaagtg cttagccctc
     7741 ccacacataa ccagagggca gcaattcacg aatcccaact gccgtcggct gtccatcact
     7801 gtccttcact atggctttga tcccaggatg cagatcgaga agcacctgtc ggcaccgtcc
     7861 gcaggggctc aagatgcccc tgttctcatt tccgatcgcg acgatacaag tcaggttgcc
     7921 agctgccgca gcagcagcag tgcccagcac cacgagttct gcacaaggtc ccccagtaaa
     7981 atgatataca ttgacaccag tgaagatgcg gccgtcgcta gagagagctg cgctggcgac
     8041 gctgtagtct tcagagatgg ggatgctgtt gattgtagcc gttgctcttt caatgagggt
     8101 ggattcttct tgagacaaag gcttggccat ggtggcaagc ttatcatcgt gtttttcaaa
     8161 ggaaaaccac gtccccgtgg ttcggggggc ctagacgttt ttttaacctc gactaaacac
     8221 atgtaaagca tgtgcaccga ggccccagat cagatcccat acaatggggt accttctggg
     8281 catccttcag ccccttgttg aatacgcttg aggagagcca tttgactctt tccacaacta
     8341 tccaactcac aacgtggcac tggggttgtg ccgcctttgc aggtgtatct tatacacgtg
     8401 gcttttggcc gcagaggcac ctgtcgccag gtggggggtt ccgctgcctg caaagggtcg
     8461 ctacagacgt tgtttgtctt caagaagctt ccagaggaac tgcttccttc acgacattca
     8521 acagaccttg cattcctttg gcgagagggg aaagacccct aggaatgctc gtcaagaaga
     8581 cagggccagg tttccgggcc ctcacattgc caaaagacgg caatatggtg gaaaataaca
     8641 tatagacaaa cgcacaccgg ccttattcca agcggcttcg gccagtaacg ttaggggggg
     8701 gggggggggg ggcggaattc gttaactgcg gccgcttaga cccaacgatt gtagggtccc
     8761 gcaacatgag tgagagagta tggggacacg gtaacaacat tgtctcgggt taaggttgat
     8821 gcaactcgag aaggaatttg gtgtgataaa ttggcttcgg gggatacatc aggacactcc
     8881 cggtctgcgg gctttttttt cgttactttt ttgcatttac acttaatttt gttgatatca
     8941 aaaccatgat taggcttgtt taggcttttg tgctggtaga atacaaggga cacacgaaaa
     9001 gggacccctc gtttggggga gcgcaaagag gtggtagcat gaagctcctt ccgagcgcac
     9061 tcaatgagca ccgagccgtg aatgggtgct atcgccacgc caccaaagga aggatcggcg
     9121 tagatctcct cactgtctga ccagaattca tcaagttgag gcaagttatc gtcctcgggg
     9181 tgctgaggct catccgcttc agggtgccgc tcatcttcca cctgacagga agccagtttc
     9241 tgagaattgc tgaggagagg gagctgctgg tttgactggt tagaagttag ctgctcagat
     9301 ggactagtga gaggctcagc atgaacggga gaatcggctg tgggaagaga ctggtgagga
     9361 tgagcagcca cctcggcagg ctgcaccgtt ccagtacatt ccccagcagc tgcattagca
     9421 ccactgtgtg ctccagaagg cattgtgcag tggagaatac tacttgtttc tgcaaaccca
     9481 cctgaggctg ttttactgta cgtacaggta gatgtttctg cggaggaagc ctgcagggga
     9541 cagaagtctg tagattcgtc tttcactagg tgagaagtag atgaggctga tgaaaagctc
     9601 ttagtgttgt gtgaacctgg ggctcccttt gggctggcag agatcctctt ccttctgaga
     9661 ccagcagtac ctgatttatt gtggttctgc ttcattttgg ccctcttccc acatcgagga
     9721 acaggctcag taaaacgtag tcgcctcttc ctggttggcc cattgacttg gatggcccca
     9781 gatttgattt tggccttcat cccttccacg gagccaaatt catcagtgtc tgcaagccgg
     9841 tatagtggca ggacgtggag ttgttcatcc tcgggacaat ttgtgtcacg gccatctgct
     9901 cgaatcaacg tacacaccac ggtgcttccg ttgtgcatgt tgtgaatgtc cttgtgagaa
     9961 tgggcacaaa aatccatgca acaggtgaca ccagagaaag gacgcccctc ttcatttcca
    10021 agtcgacagt ctccagcaac ttcttcatat tccacctgat tttgataagc aactggagcc
    10081 atctgcttgt aaagtggagc taatactgta gccaattctt gtaaattctt ttcaagctgt
    10141 ttttcctctc tgcttatgag ggtcttgcgg tctggaatga tccacccggt tttcacgtca
    10201 cttccttcag aactcattcc agtaattctc ttatagtagt tatgtaaggg gtagtttgga
    10261 gcaagtctga attttctggg gttttcactc ctctgttcct tcaatccagc tatcaggttt
    10321 tttgcaggct tgctaagtaa gtccatgatg ctctctttaa attctgactg tgcactgtct
    10381 gtggtgggac ccagtggtcc attgcttgat gtttgagctt cacgcttctc cgtaacaact
    10441 gtaattccat cgaaatgtaa cactttccct ttcctgagga ttgatgctgg gtctggcaag
    10501 agtgtgtcag gggaggtacc aggcaacgtc ggtggtggta tctgatgcac caggttctct
    10561 tgagcgccta gatgaaactg tgtttgggca gcaccagcca ttatattagc acactggtta
    10621 gatggtgggg actgg
//
