LOCUS       sequence-47703-e         821 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Young CL, Raden DL, Caplan JL, Czymmek KJ, Robinson AS
  TITLE     Cassette series designed for live-cell imaging of proteins and 
            high-resolution techniques in yeast.
  JOURNAL   Yeast. 2012 Mar;29(3-4):119-36. doi: 10.1002/yea.2895. Epub 2012 Apr
            4.
  PUBMED    22473760
REFERENCE   2  (bases 1 to 821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast."; 
            date: "2012-03"; volume: "29(3-4):119-36. doi"; pages: " 
            10.1002/yea.2895. Epub 2012 Apr 4"
COMMENT     Sequence Label: pCY 3120-04
FEATURES             Location/Qualifiers
     source          1..821
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(118..792)
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
ORIGIN
        1 tatcatcgat gaattcgagc tcgggtattt tctccttacg catctgtgcg gtatttcaca
       61 ccgcatagat cggcaagtgc acaaacaata cttaaataaa tactactcag taataaccta
      121 tttcttagca tttttgacga aatttgctat tttgttagag tcttttacac catttgtctc
      181 cacacctccg cttacatcaa caccaataac gccatttaat ctaagcgcat caccaacatt
      241 ttctggcgtc agtccaccag ctaacataaa atgtaagctt tcggggctct cttgccttcc
      301 aacccagtca gaaatcgagt tccaatccaa aagttcacct gtcccacctg cttctgaatc
      361 aaacaaggga ataaacgaat gaggtttctg tgaagctgca ctgagtagta tgttgcagtc
      421 ttttggaaat acgagtcttt taataactgg caaaccgagg aactcttggt attcttgcca
      481 cgactcatct ccatgcagtt ggacgatatc aatgccgtaa tcattgacca gagccaaaac
      541 atcctcctta ggttgattac gaaacacgcc aaccaagtat ttcggagtgc ctgaactatt
      601 tttatatgct tttacaagac ttgaaatttt ccttgcaata accgggtcaa ttgttctctt
      661 tctattgggc acacatataa tacccagcaa gtcagcatcg gaatctagag cacattctgc
      721 ggcctctgtg ctctgcaagc cgcaaacttt caccaatgga ccagaactac ctgtgaaatt
      781 aataacagac atactccaag ctgcctttgt gtgcttaatc a
//
