LOCUS       sequence-477041-       14655 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression vector of Ribozyme split GFPuv with Triplex motif.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14655)
  AUTHORS   Liu Y, Rajput R, Islam MT, Valle ID, Yao T, Agrawal R, Boone BA, 
            Eckert CA, Abraham PE, Chen JG, Tuskan GA, Yang X
  TITLE     A split ribozyme system for in vivo plant RNA imaging and genetic 
            engineering.
  JOURNAL   Plant Biotechnol J. 2025 May;23(5):1640-1649. doi: 
            10.1111/pbi.14612. Epub 2025 Feb 7.
  PUBMED    39919021
REFERENCE   2  (bases 1 to 14655)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/pbi.14612";
            journalName: "Plant Biotechnol J"; date: "2025-05"; volume: "23"; 
            issue: "5"; pages: "1640-1649"
COMMENT     Sequence Label: pXYB112
FEATURES             Location/Qualifiers
     source          1..14655
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(16..361)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     723..745
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     737..754
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     738..754
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1269..1614
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     terminator      2923..3171
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     primer_bind     complement(3860..3876)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3860..3876)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3873..3895)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3884..3900
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3908..3914,3915..3932,3933..3938))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3953..3974
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        4165..4841
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     CDS             4909..5706
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLL
                     SSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEH
                     QGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDI
                     ALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4966..4985)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5576..5595
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5746..5920
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    5998..6022
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6447..7241
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      7328..7916
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7817..7836
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8070..8087
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8102..8242
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8168..8187)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8328..8350
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8586..8780
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8846..9919)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10348..10977)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    12278..12302
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     terminator      complement(12324..12576)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(12608..12625)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(join(12632..14449,14640..14654))
                     /codon_start=1
                     /product="beta-glucuronidase"
                     /label=GUSPlus(TM)
                     /note="codon-optimized Staphylococcus gusA gene with a 
                     catalase intron to ensure expression in plants but not 
                     bacteria"
                     /translation="MVDLRNRRTSLYPINTETRGVFDLNGVWNFKLDYGKGLEEKWYES
                     KLTDTISMAVPSSYNDIGVTKEIRNHIGYVWYEREFTVPAYLKDQRIVLRFGSATHKAI
                     VYVNGELVVEHKGGFLPFEAEINNSLRDGMNRVTVAVDNILDDSTLPVGLYSERHEEGL
                     GKVIRNKPNFDFFNYAGLHRPVKIYTTPFTYVEDISVVTDFNGPTGTVTYTVDFQGKAE
                     TVKVSVVDEEGKVVASTEGLSGNVEIPNVILWEPLNTYLYQIKVELVNDGLTIDVYEEP
                     FGVRTVEVNDGKFLINNKPFYFKGFGKHEDTPINGRGFNEASNVMDFNILKWIGANSFR
                     TAHYPYSEELMRLADREGLVVIDETPAVGVHLNFMATTGLGEGSERVSTWEKIRTFEHH
                     QDVLRELVSRDKNHPSVVMWSIANEAATEEEGAYEYFKPLVELTKELDPQKRPVTIVLF
                     VMATPETDKVAELIDVIALNRYNGWYFDGGDLEAAKVHLRQEFHAWNKRCPGKPIMITE
                     YGADTVAGFHDIDPVMFTEEYQVEYYQANHVVFDEFENFVGEQAWNFADFATSQGVMRV
                     QGNKKGVFTRDRKPKLAAHVFRERWTNIPDFGYKN"
     intron          14450..14639
                     /label=cat1 intron
                     /note="castor bean catalase intron, modified"
ORIGIN
        1 gtcaagagtc ccccgtgttc tctccaaatg aaatgaactt ccttatatag aggaagggtc
       61 ttgcgaagga tagtgggatt gtgcgtcatc ccttacgtca gtggagatat cacatcaatc
      121 cacttgcttt gaagacgtgg ttggaacgtc ttctttttcc acgatgctcc tcgtgggtgg
      181 gggtccatct ttgggaccac tgtcggcaga ggcatcttca acgatggcct ttcctttatc
      241 gcaatgatgg catttgtagg agccaccttc cttttccact atcttcacaa taaagtgaca
      301 gatagctggg caatggaatc cgaggaggtt tccggatatt accctttgtt gaaaagtctc
      361 aattgccctt tggtcttctg agactgtatc tttgatattt ttggagtaga caagtgtgtc
      421 gtgctccacc atgttgacga agattttctt cttgtcattg agtcgtaaga gactctgtat
      481 gaactgttcg ccagtcttta cggcgagttc tgttaggtcc tctatttgaa tctttgactc
      541 catgaagcta aactgaaggc gggaaacgac aatctgatcc aagctcaagc tgctctagca
      601 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
      661 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt
      721 ttcccagtca cgacgttgta aaacgacggc cagtgccaag cttgcatgcc tgcaggtccc
      781 cagattagcc ttttcaattt cagaaagaat gctaacccac agatggttag agaggcttac
      841 gcagcaggtc tcatcaagac gatctacccg agcaataatc tccaggaaat caaatacctt
      901 cccaagaagg ttaaagatgc agtcaaaaga ttcaggacta actgcatcaa gaacacagag
      961 aaagatatat ttctcaagat cagaagtact attccagtat ggacgattca aggcttgctt
     1021 cacaaaccaa ggcaagtaat agagattgga gtctctaaaa aggtagttcc cactgaatca
     1081 aaggccatgg agtcaaagat tcaaatagag gacctaacag aactcgccgt aaagactggc
     1141 gaacagttca tacagagtct cttacgactc aatgacaaga agaaaatctt cgtcaacatg
     1201 gtggagcacg acacacttgt ctactccaaa aatatcaaag atacagtctc agaagaccaa
     1261 agggcaattg agacttttca acaaagggta atatccggaa acctcctcgg attccattgc
     1321 ccagctatct gtcactttat tgtgaagata gtggaaaagg aaggtggctc ctacaaatgc
     1381 catcattgcg ataaaggaaa ggccatcgtt gaagatgcct ctgccgacag tggtcccaaa
     1441 gatggacccc cacccacgag gagcatcgtg gaaaaagaag acgttccaac cacgtcttca
     1501 aagcaagtgg attgatgtga tatctccact gacgtaaggg atgacgcaca atcccactat
     1561 ccttcgcaag acccttcctc tatataagga agttcatttc atttggagag aacacggggg
     1621 actcaaacat tactcattca caaaaccatc ttaaagcaac tacacaagtc ttgaaatttt
     1681 ctcatatttt ctatttacta tataaacttt taatcaaatc aagattaaag ttaattaaat
     1741 gacaaccttc aaaatcgagt cccggatcca cggcaacctc aacggggaga agttcgagtt
     1801 ggttggaggt ggagtaggtg aggagggtcg cctcgagatt gagatgaaga ctaaagataa
     1861 accactggca ttctctccct tcctgctgac cacttgcatg ggttacgggt tctaccactt
     1921 cgccagcttc ccaaagggga ttaagaacat ctataaatag caatatttac ctttgggtca
     1981 aaaagttatc aggcatgcac ctggtagcta gtctttaaac caatagattg catcggttta
     2041 aaaggcaaga ccgtcaaatt gcgggaaagg ggtcaacagc cgttcagtac caagtctcag
     2101 gggaaacttt gagatggcct tgcaaagggt atggtaataa gctgacggac atggtcctaa
     2161 ccacgcagcc aagtcctaag tcaacagatc ttctgttgat atggatgcag ttcacagact
     2221 aaatgtcggt cggggaagat gtattcttct cataagatat agtcggacct ctccttaatg
     2281 ggagctagcg gatgaagtga tgcaacactg gagccgctgg gaactaattt gtatgcgaaa
     2341 gtatattgat tagttttgga gtactcgctt catgctgcaa cgaacggagg ttacaccaac
     2401 accaggaagg agatctatga agacggcggc atcttggagg tcaacttccg ttacacttac
     2461 gagttcaaca agatcatcgg tgacgtcgag tgcattggac atggattccc aagtcagagt
     2521 ccgatcttca aggacacgat cgtgaagagt tgtcccacgg tggacctgat gttgccaatg
     2581 tccgggaaca tcatcgccag ctcctacgct tacgccttcc aactgaagga cggctctttc
     2641 tacacggcag aagtcaagaa caacatagac ttcaagaatc caatccacga gtccttctcg
     2701 aagtcagggc ccatgttcac ccacagacgt gtcgaggaga ctctcaccaa ggagaacctt
     2761 gccatagtgg agtaccagca ggttttcaac agcgccccaa gagacatgta ggattcgtca
     2821 gtagggttgt aaaggttttt cttttcctga gaaaacaacc ttttgttttc tcaggttttg
     2881 ctttttggcc tttccctagc tttaaaaaaa aaaaagcaaa aatatgaaga tgaagatgaa
     2941 atatttggtg tgtcaaataa aaagcttgtg tgcttaagtt tgtgtttttt tcttggcttg
     3001 ttgtgttatg aatttgtggc tttttctaat attaaatgaa tgtaagatct cattataatg
     3061 aataaacaaa tgtttctata atccattgtg aatgttttgt tggatctctt ctgcagcata
     3121 taactactgt atgtgctatg gtatggacta tggaatatga ttaaagataa gatgggctca
     3181 tagagtaaaa cgaggcgagg gacctataaa cctcccttca tcatgctatt tcatgatcta
     3241 ttttataaaa taaagatgta gaaaaaagta agcgtaataa ccgcaaaaca aatgatttaa
     3301 aacatggcac ataatgagga gattaagttc ggtttacgtt tattttagta ctaattgtaa
     3361 cgtgagacta cgtatcggga atcgcctaat taaagcatta atgcgaacct gattagattc
     3421 accgaccctc ctatcgtgtc gacctttctg tttcttagaa ttttttggta gtctatgtac
     3481 taataatgtc agcttcgtat ttatttcata agcaatttgc atttgcaatt tgttttttac
     3541 ttttattttt attgtattgt ggaatgtgga ctcgtaccaa catgaagtta tataccacca
     3601 aaaaaattac agttagtcaa aagattcacg agtgagagct acttatgatt gtcttttacg
     3661 tatatgtcta attgtctatt tgctcaataa tctttgtact ttcttttgtc gttgataaaa
     3721 tcacaaagtt ccaaaagtaa tcgaatgatt tgcttttaag aaaagaagag ctcaataatt
     3781 caacatatat ctgtacacaa ttaccgcctt tgagtgagct ctagatgcca cctgacgtct
     3841 aagaagaatt cgtaatcatg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     3901 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     3961 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     4021 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attggctaga
     4081 gcagcttgcc aacatggtgg agcacgacac tctcgtctac tccaagaata tcaaagatac
     4141 agtctcagaa gaccaaaggg ctattgagac ttttcaacaa agggtaatat cgggaaacct
     4201 cctcggattc cattgcccag ctatctgtca cttcatcaaa aggacagtag aaaaggaagg
     4261 tggcacctac aaatgccatc attgcgataa aggaaaggct atcgttcaag atgcctctgc
     4321 cgacagtggt cccaaagatg gacccccacc cacgaggagc atcgtggaaa aagaagacgt
     4381 tccaaccacg tcttcaaagc aagtggattg atgtgaacat ggtggagcac gacactctcg
     4441 tctactccaa gaatatcaaa gatacagtct cagaagacca aagggctatt gagacttttc
     4501 aacaaagggt aatatcggga aacctcctcg gattccattg cccagctatc tgtcacttca
     4561 tcaaaaggac agtagaaaag gaaggtggca cctacaaatg ccatcattgc gataaaggaa
     4621 aggctatcgt tcaagatgcc tctgccgaca gtggtcccaa agatggaccc ccacccacga
     4681 ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc aaagcaagtg gattgatgtg
     4741 atatctccac tgacgtaagg gatgacgcac aatcccacta tccttcgcaa gacccttcct
     4801 ctatataagg aagttcattt catttggaga ggacacgctg aaatcaccag tctctctcta
     4861 caaatctatc tctctcgagc tttcgcagat cccggggggc aatgagatat ggggattgaa
     4921 caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac
     4981 tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg
     5041 cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact ccaggacgag
     5101 gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt
     5161 gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg
     5221 tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg
     5281 catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga
     5341 gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag
     5401 gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat
     5461 ctcgtcgtga cacatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt
     5521 tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg
     5581 gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt
     5641 tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc
     5701 ttctgaagta gatgccgacc ggatctgtcg atcgacaagc tcgagtttct ccataataat
     5761 gtgtgagtag ttcccagata agggaattag ggttcctata gggtttcgct catgtgttga
     5821 gcatataaga aacccttagt atgtatttgt atttgtaaaa tacttctatc aataaaattt
     5881 ctaattccta aaaccaaaat ccagtactaa aatccagatc ccccgaatta attcggcgtt
     5941 aattcagtac attaaaaacg tccgcaatgt gttattaagt tgtctaagcg tcaatttgtt
     6001 tacaccacaa tatatcctgc caccagccag ccaacagctc cccgaccggc agctcggcac
     6061 aaaatcacca ctcgatacag gcagcccatc agtccgggac ggcgtcagcg ggagagccgt
     6121 tgtaaggcgg cagactttgc tcatgttacc gatgctattc ggaagaacgg caactaagct
     6181 gccgggtttg aaacacggat gatctcgcgg agggtagcat gttgattgta acgatgacag
     6241 agcgttgctg cctgtgatca ccgcggtttc aaaatcggct ccgtcgatac tatgttatac
     6301 gccaactttg aaaacaactt tgaaaaagct gttttctggt atttaaggtt ttagaatgca
     6361 aggaacagtg aattggagtt cgtcttgtta taattagctt cttggggtat ctttaaatac
     6421 tgtagaaaag aggaaggaaa taataaatgg ctaaaatgag aatatcaccg gaattgaaaa
     6481 aactgatcga aaaataccgc tgcgtaaaag atacggaagg aatgtctcct gctaaggtat
     6541 ataagctggt gggagaaaat gaaaacctat atttaaaaat gacggacagc cggtataaag
     6601 ggaccaccta tgatgtggaa cgggaaaagg acatgatgct atggctggaa ggaaagctgc
     6661 ctgttccaaa ggtcctgcac tttgaacggc atgatggctg gagcaatctg ctcatgagtg
     6721 aggccgatgg cgtcctttgc tcggaagagt atgaagatga acaaagccct gaaaagatta
     6781 tcgagctgta tgcggagtgc atcaggctct ttcactccat cgacatatcg gattgtccct
     6841 atacgaatag cttagacagc cgcttagccg aattggatta cttactgaat aacgatctgg
     6901 ccgatgtgga ttgcgaaaac tgggaagaag acactccatt taaagatccg cgcgagctgt
     6961 atgatttttt aaagacggaa aagcccgaag aggaacttgt cttttcccac ggcgacctgg
     7021 gagacagcaa catctttgtg aaagatggca aagtaagtgg ctttattgat cttgggagaa
     7081 gcggcagggc ggacaagtgg tatgacattg ccttctgcgt ccggtcgatc agggaggata
     7141 tcggggaaga acagtatgtc gagctatttt ttgacttact ggggatcaag cctgattggg
     7201 agaaaataaa atattatatt ttactggatg aattgtttta gtacctagaa tgcatgacca
     7261 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     7321 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     7381 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     7441 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc
     7501 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     7561 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     7621 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     7681 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     7741 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     7801 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     7861 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     7921 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     7981 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     8041 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     8101 gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatggtgca
     8161 ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct
     8221 acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg
     8281 ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat
     8341 gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagggtgcct tgatgtgggc
     8401 gccggcggtc gagtggcgac ggcgcggctt gtccgcgccc tggtagattg cctggccgta
     8461 ggccagccat ttttgagcgg ccagcggccg cgataggccg acgcgaagcg gcggggcgta
     8521 gggagcgcag cgaccgaagg gtaggcgctt tttgcagctc ttcggctgtg cgctggccag
     8581 acagttatgc acaggccagg cgggttttaa gagttttaat aagttttaaa gagttttagg
     8641 cggaaaaatc gccttttttc tcttttatat cagtcactta catgtgtgac cggttcccaa
     8701 tgtacggctt tgggttccca atgtacgggt tccggttccc aatgtacggc tttgggttcc
     8761 caatgtacgt gctatccaca ggaaagagac cttttcgacc tttttcccct gctagggcaa
     8821 tttgccctag catctgctcc gtacattagg aaccggcgga tgcttcgccc tcgatcaggt
     8881 tgcggtagcg catgactagg atcgggccag cctgccccgc ctcctccttc aaatcgtact
     8941 ccggcaggtc atttgacccg atcagcttgc gcacggtgaa acagaacttc ttgaactctc
     9001 cggcgctgcc actgcgttcg tagatcgtct tgaacaacca tctggcttct gccttgcctg
     9061 cggcgcggcg tgccaggcgg tagagaaaac ggccgatgcc gggatcgatc aaaaagtaat
     9121 cggggtgaac cgtcagcacg tccgggttct tgccttctgt gatctcgcgg tacatccaat
     9181 cagctagctc gatctcgatg tactccggcc gcccggtttc gctctttacg atcttgtagc
     9241 ggctaatcaa ggcttcaccc tcggataccg tcaccaggcg gccgttcttg gccttcttcg
     9301 tacgctgcat ggcaacgtgc gtggtgttta accgaatgca ggtttctacc aggtcgtctt
     9361 tctgctttcc gccatcggct cgccggcaga acttgagtac gtccgcaacg tgtggacgga
     9421 acacgcggcc gggcttgtct cccttccctt cccggtatcg gttcatggat tcggttagat
     9481 gggaaaccgc catcagtacc aggtcgtaat cccacacact ggccatgccg gccggccctg
     9541 cggaaacctc tacgtgcccg tctggaagct cgtagcggat cacctcgcca gctcgtcggt
     9601 cacgcttcga cagacggaaa acggccacgt ccatgatgct gcgactatcg cgggtgccca
     9661 cgtcatagag catcggaacg aaaaaatctg gttgctcgtc gcccttgggc ggcttcctaa
     9721 tcgacggcgc accggctgcc ggcggttgcc gggattcttt gcggattcga tcagcggccg
     9781 cttgccacga ttcaccgggg cgtgcttctg cctcgatgcg ttgccgctgg gcggcctgcg
     9841 cggccttcaa cttctccacc aggtcatcac ccagcgccgc gccgatttgt accgggccgg
     9901 atggtttgcg accgctcacg ccgattcctc gggcttgggg gttccagtgc cattgcaggg
     9961 ccggcaggca acccagccgc ttacgcctgg ccaaccgccc gttcctccac acatggggca
    10021 ttccacggcg tcggtgcctg gttgttcttg attttccatg ccgcctcctt tagccgctaa
    10081 aattcatcta ctcatttatt catttgctca tttactctgg tagctgcgcg atgtattcag
    10141 atagcagctc ggtaatggtc ttgccttggc gtaccgcgta catcttcagc ttggtgtgat
    10201 cctccgccgg caactgaaag ttgacccgct tcatggctgg cgtgtctgcc aggctggcca
    10261 acgttgcagc cttgctgctg cgtgcgctcg gacggccggc acttagcgtg tttgtgcttt
    10321 tgctcatttt ctctttacct cattaactca aatgagtttt gatttaattt cagcggccag
    10381 cgcctggacc tcgcgggcag cgtcgccctc gggttctgat tcaagaacgg ttgtgccggc
    10441 ggcggcagtg cctgggtagc tcacgcgctg cgtgatacgg gactcaagaa tgggcagctc
    10501 gtacccggcc agcgcctcgg caacctcacc gccgatgcgc gtgcctttga tcgcccgcga
    10561 cacgacaaag gccgcttgta gccttccatc cgtgacctca atgcgctgct taaccagctc
    10621 caccaggtcg gcggtggccc atatgtcgta agggcttggc tgcaccggaa tcagcacgaa
    10681 gtcggctgcc ttgatcgcgg acacagccaa gtccgccgcc tggggcgctc cgtcgatcac
    10741 tacgaagtcg cgccggccga tggccttcac gtcgcggtca atcgtcgggc ggtcgatgcc
    10801 gacaacggtt agcggttgat cttcccgcac ggccgcccaa tcgcgggcac tgccctgggg
    10861 atcggaatcg actaacagaa catcggcccc ggcgagttgc agggcgcggg ctagatgggt
    10921 tgcgatggtc gtcttgcctg acccgccttt ctggttaagt acagcgataa ccttcatgcg
    10981 ttccccttgc gtatttgttt atttactcat cgcatcatat acgcagcgac cgcatgacgc
    11041 aagctgtttt actcaaatac acatcacctt tttagacggc ggcgctcggt ttcttcagcg
    11101 gccaagctgg ccggccaggc cgccagcttg gcatcagaca aaccggccag gatttcatgc
    11161 agccgcacgg ttgagacgtg cgcgggcggc tcgaacacgt acccggccgc gatcatctcc
    11221 gcctcgatct cttcggtaat gaaaaacggt tcgtcctggc cgtcctggtg cggtttcatg
    11281 cttgttcctc ttggcgttca ttctcggcgg ccgccagggc gtcggcctcg gtcaatgcgt
    11341 cctcacggaa ggcaccgcgc cgcctggcct cggtgggcgt cacttcctcg ctgcgctcaa
    11401 gtgcgcggta cagggtcgag cgatgcacgc caagcagtgc agccgcctct ttcacggtgc
    11461 ggccttcctg gtcgatcagc tcgcgggcgt gcgcgatctg tgccggggtg agggtagggc
    11521 gggggccaaa cttcacgcct cgggccttgg cggcctcgcg cccgctccgg gtgcggtcga
    11581 tgattaggga acgctcgaac tcggcaatgc cggcgaacac ggtcaacacc atgcggccgg
    11641 ccggcgtggt ggtgtcggcc cacggctctg ccaggctacg caggcccgcg ccggcctcct
    11701 ggatgcgctc ggcaatgtcc agtaggtcgc gggtgctgcg ggccaggcgg tctagcctgg
    11761 tcactgtcac aacgtcgcca gggcgtaggt ggtcaagcat cctggccagc tccgggcggt
    11821 cgcgcctggt gccggtgatc ttctcggaaa acagcttggt gcagccggcc gcgtgcagtt
    11881 cggcccgttg gttggtcaag tcctggtcgt cggtgctgac gcgggcatag cccagcaggc
    11941 cagcggcggc gctcttgttc atggcgtaat gtctccggtt ctagtcgcaa gtattctact
    12001 ttatgcgact aaaacacgcg acaagaaaac gccaggaaaa gggcagggcg gcagcctgtc
    12061 gcgtaactta ggacttgtgc gacatgtcgt tttcagaaga cggctgcact gaacgtcaga
    12121 agccgactgc actatagcag cggaggggtt ggatcaaagt actttgatcc cgaggggaac
    12181 cctgtggttg gcatgcacat acaaatggac gaacggataa accttttcac gcccttttaa
    12241 atatccgatt attctaataa acgctctttt ctcttaggtt tacccgccaa tatatcctgt
    12301 caaacactga tagtttaatt cccgatctag taacatagat gacaccgcgc gcgataattt
    12361 atcctagttt gcgcgctata ttttgttttc tatcgcgtat taaatgtata attgcgggac
    12421 tctaatcata aaaacccatc tcataaataa cgtcatgcat tacatgttaa ttattacatg
    12481 cttaacgtaa ttcaacagaa attatatgat aatcatcgca agaccggcaa caggattcaa
    12541 tcttaagaaa ctttattgcc aaatgtttga acgatcgggg aaattcgagc tggtcaccaa
    12601 ttcacacgtg atggtgatgg tgatggctag cgttcttgta gccgaaatct ggaatgttgg
    12661 tccagcgctc gcgaaagacg tgcgcggcga gcttcggctt gcggtcacga gtgaacacgc
    12721 ccttcttgtt tccttggacg cgcatcacgc cctgagaggt cgcgaagtcc gcgaagttcc
    12781 acgcttgctc acccacgaag ttctcaaact catcgaacac gacgtggttc gcctggtagt
    12841 actcgacttg atattcctcg gtgaacatca ctggatcaat gtcgtgaaag cccgcaacgg
    12901 tgtctgcgcc gtactcagtg atcatgatcg gctttcctgg gcaacgcttg ttccacgcgt
    12961 gaaattcctg gcggagatgg actttggccg cttcgagatc accgccatcg aagtaccatc
    13021 cgttatagcg attgagcgcg atgacgtcaa tcagttcggc gactttgtcc gtctccgggg
    13081 tagccatcac aaacagcacg atcgtgaccg gacgcttctg tgggtcgagt tccttggtca
    13141 gctccaccaa cggcttgaag tactcgtacg cgccctcttc ctcagtcgcc gcctcgttgg
    13201 cgatgctcca catcacgacg cttggatggt tcttgtcacg agacaccagt tcacggagaa
    13261 cgtcttgatg gtgctcaaac gtccgaatct tctcccaggt actgacgcgc tcgctgcctt
    13321 cgccgagtcc cgtggtggcc atgaagttga ggtgcacgcc aactgccgga gtctcgtcga
    13381 tcacgaccag accctcgcga tccgcaagac gcatcaactc ttcagagtac ggatagtgtg
    13441 cggtccggaa gctgttggcg ccgatccatt tgaggatatt gaaatccatc acattgctcg
    13501 cttcgttaaa gccacggccg ttgataggag tgtcctcatg tttgccaaag cccttgaagt
    13561 agaacggttt gttgttgatg aggaacttgc cgtcgttgac ttccacggtc cgcacgccga
    13621 acggctcttc atagacatcg atggtcagtc cgtcgttcac cagttccact ttgatctggt
    13681 agagatacgt gttcagtggt tcccagagga tgacattcgg aatctccacg ttaccgctca
    13741 ggccctcggt gcttgcgacc actttgcctt cctcatccac gaccgacact ttcacggtct
    13801 cggctttgcc ttgaaagtcc accgtatagg tcacagtccc ggttgggcca ttgaagtcgg
    13861 tcacaaccga gatgtcctcg acgtacgtaa acggggtcgt gtagattttc accggacggt
    13921 gcaggcctgc atagttgaag aagtcgaagt tcggcttgtt acgaatgact tttccgaggc
    13981 cctcttcgtg gcgctcgctg tacagcccca ccgggagggt gctatcgtcg aggatgttgt
    14041 ccacggcgac ggtgacgcga ttcatgccat cacgcagcga gttgttgatt tccgcttcga
    14101 atggcaggaa tccgcccttg tgctccacga ccagctcacc attgacatag acaattgctt
    14161 tgtgagttgc agagccgaag cggagcacga tacgctgatc cttcagatag gccggcaccg
    14221 tgaactcacg ttcgtaccag acatatccga tatggttgcg gatttccttg gtcacgccaa
    14281 tgtcattgta actgcttggg acggccatac taatagtgtc ggtcagcttg ctttcgtacc
    14341 acttctcttc cagtcctttc ccgtagtcca gcttgaagtt ccagacgcca ttgaggtcga
    14401 agacgccacg ggtctcggtg ttgatcgggt acagactagt tcgtcggttc tgtaactatc
    14461 atcatcatca tagacacacg aaataaagta atcagattat cagttaaagc tatgtaatat
    14521 ttacaccata accaatcaat taaaaaatag atcagtttaa agaaagatca aagctcaaaa
    14581 aaataaaaag agaaaagggt cctaaccaag aaaatgaagg agaaaaacta gaaatttacc
    14641 ctcagatcta ccatg
//
