LOCUS       sequence-477042-       14170 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression vector of GFPuv with 3' tEvopreq motif.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14170)
  AUTHORS   Liu Y, Rajput R, Islam MT, Valle ID, Yao T, Agrawal R, Boone BA, 
            Eckert CA, Abraham PE, Chen JG, Tuskan GA, Yang X
  TITLE     A split ribozyme system for in vivo plant RNA imaging and genetic 
            engineering.
  JOURNAL   Plant Biotechnol J. 2025 May;23(5):1640-1649. doi: 
            10.1111/pbi.14612. Epub 2025 Feb 7.
  PUBMED    39919021
REFERENCE   2  (bases 1 to 14170)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/pbi.14612";
            journalName: "Plant Biotechnol J"; date: "2025-05"; volume: "23"; 
            issue: "5"; pages: "1640-1649"
COMMENT     Sequence Label: pXYB105
FEATURES             Location/Qualifiers
     source          1..14170
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(16..361)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     723..745
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     737..754
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     738..754
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1269..1614
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     terminator      2437..2685
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     primer_bind     complement(3375..3391)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3375..3391)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3388..3410)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3399..3415
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3423..3429,3430..3447,3448..3453))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3468..3489
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        3680..4356
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     CDS             4424..5221
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLL
                     SSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEH
                     QGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDI
                     ALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4481..4500)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5091..5110
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5261..5435
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    5513..5537
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             5962..6756
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      6843..7431
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7332..7351
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7585..7602
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7617..7757
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7683..7702)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7843..7865
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8101..8295
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8361..9434)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(9863..10492)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    11793..11817
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     terminator      complement(11839..12091)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(12123..12140)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(join(12147..13964,14155..14169))
                     /codon_start=1
                     /product="beta-glucuronidase"
                     /label=GUSPlus(TM)
                     /note="codon-optimized Staphylococcus gusA gene with a 
                     catalase intron to ensure expression in plants but not 
                     bacteria"
                     /translation="MVDLRNRRTSLYPINTETRGVFDLNGVWNFKLDYGKGLEEKWYES
                     KLTDTISMAVPSSYNDIGVTKEIRNHIGYVWYEREFTVPAYLKDQRIVLRFGSATHKAI
                     VYVNGELVVEHKGGFLPFEAEINNSLRDGMNRVTVAVDNILDDSTLPVGLYSERHEEGL
                     GKVIRNKPNFDFFNYAGLHRPVKIYTTPFTYVEDISVVTDFNGPTGTVTYTVDFQGKAE
                     TVKVSVVDEEGKVVASTEGLSGNVEIPNVILWEPLNTYLYQIKVELVNDGLTIDVYEEP
                     FGVRTVEVNDGKFLINNKPFYFKGFGKHEDTPINGRGFNEASNVMDFNILKWIGANSFR
                     TAHYPYSEELMRLADREGLVVIDETPAVGVHLNFMATTGLGEGSERVSTWEKIRTFEHH
                     QDVLRELVSRDKNHPSVVMWSIANEAATEEEGAYEYFKPLVELTKELDPQKRPVTIVLF
                     VMATPETDKVAELIDVIALNRYNGWYFDGGDLEAAKVHLRQEFHAWNKRCPGKPIMITE
                     YGADTVAGFHDIDPVMFTEEYQVEYYQANHVVFDEFENFVGEQAWNFADFATSQGVMRV
                     QGNKKGVFTRDRKPKLAAHVFRERWTNIPDFGYKN"
     intron          13965..14154
                     /label=cat1 intron
                     /note="castor bean catalase intron, modified"
ORIGIN
        1 gtcaagagtc ccccgtgttc tctccaaatg aaatgaactt ccttatatag aggaagggtc
       61 ttgcgaagga tagtgggatt gtgcgtcatc ccttacgtca gtggagatat cacatcaatc
      121 cacttgcttt gaagacgtgg ttggaacgtc ttctttttcc acgatgctcc tcgtgggtgg
      181 gggtccatct ttgggaccac tgtcggcaga ggcatcttca acgatggcct ttcctttatc
      241 gcaatgatgg catttgtagg agccaccttc cttttccact atcttcacaa taaagtgaca
      301 gatagctggg caatggaatc cgaggaggtt tccggatatt accctttgtt gaaaagtctc
      361 aattgccctt tggtcttctg agactgtatc tttgatattt ttggagtaga caagtgtgtc
      421 gtgctccacc atgttgacga agattttctt cttgtcattg agtcgtaaga gactctgtat
      481 gaactgttcg ccagtcttta cggcgagttc tgttaggtcc tctatttgaa tctttgactc
      541 catgaagcta aactgaaggc gggaaacgac aatctgatcc aagctcaagc tgctctagca
      601 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
      661 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt
      721 ttcccagtca cgacgttgta aaacgacggc cagtgccaag cttgcatgcc tgcaggtccc
      781 cagattagcc ttttcaattt cagaaagaat gctaacccac agatggttag agaggcttac
      841 gcagcaggtc tcatcaagac gatctacccg agcaataatc tccaggaaat caaatacctt
      901 cccaagaagg ttaaagatgc agtcaaaaga ttcaggacta actgcatcaa gaacacagag
      961 aaagatatat ttctcaagat cagaagtact attccagtat ggacgattca aggcttgctt
     1021 cacaaaccaa ggcaagtaat agagattgga gtctctaaaa aggtagttcc cactgaatca
     1081 aaggccatgg agtcaaagat tcaaatagag gacctaacag aactcgccgt aaagactggc
     1141 gaacagttca tacagagtct cttacgactc aatgacaaga agaaaatctt cgtcaacatg
     1201 gtggagcacg acacacttgt ctactccaaa aatatcaaag atacagtctc agaagaccaa
     1261 agggcaattg agacttttca acaaagggta atatccggaa acctcctcgg attccattgc
     1321 ccagctatct gtcactttat tgtgaagata gtggaaaagg aaggtggctc ctacaaatgc
     1381 catcattgcg ataaaggaaa ggccatcgtt gaagatgcct ctgccgacag tggtcccaaa
     1441 gatggacccc cacccacgag gagcatcgtg gaaaaagaag acgttccaac cacgtcttca
     1501 aagcaagtgg attgatgtga tatctccact gacgtaaggg atgacgcaca atcccactat
     1561 ccttcgcaag acccttcctc tatataagga agttcatttc atttggagag aacacggggg
     1621 actcaaacat tactcattca caaaaccatc ttaaagcaac tacacaagtc ttgaaatttt
     1681 ctcatatttt ctatttacta tataaacttt taatcaaatc aagattaaag ttaattaaat
     1741 gacaaccttc aaaatcgagt cccggatcca cggcaacctc aacggggaga agttcgagtt
     1801 ggttggaggt ggagtaggtg aggagggtcg cctcgagatt gagatgaaga ctaaagataa
     1861 accactggca ttctctccct tcctgctgac cacttgcatg ggttacgggt tctaccactt
     1921 cgccagcttc ccaaagggga ttaagaacat ctatcttcat gctgcaacga acggaggtta
     1981 caccaacacc aggaaggaga tctatgaaga cggcggcatc ttggaggtca acttccgtta
     2041 cacttacgag ttcaacaaga tcatcggtga cgtcgagtgc attggacatg gattcccaag
     2101 tcagagtccg atcttcaagg acacgatcgt gaagagttgt cccacggtgg acctgatgtt
     2161 gccaatgtcc gggaacatca tcgccagctc ctacgcttac gccttccaac tgaaggacgg
     2221 ctctttctac acggcagaag tcaagaacaa catagacttc aagaatccaa tccacgagtc
     2281 cttctcgaag tcagggccca tgttcaccca cagacgtgtc gaggagactc tcaccaagga
     2341 gaaccttgcc atagtggagt accagcaggt tttcaacagc gccccaagag acatgtagcg
     2401 cggttctatc tagttacgcg ttaaaccaac tagaaatatg aagatgaaga tgaaatattt
     2461 ggtgtgtcaa ataaaaagct tgtgtgctta agtttgtgtt tttttcttgg cttgttgtgt
     2521 tatgaatttg tggctttttc taatattaaa tgaatgtaag atctcattat aatgaataaa
     2581 caaatgtttc tataatccat tgtgaatgtt ttgttggatc tcttctgcag catataacta
     2641 ctgtatgtgc tatggtatgg actatggaat atgattaaag ataagatggg ctcatagagt
     2701 aaaacgaggc gagggaccta taaacctccc ttcatcatgc tatttcatga tctattttat
     2761 aaaataaaga tgtagaaaaa agtaagcgta ataaccgcaa aacaaatgat ttaaaacatg
     2821 gcacataatg aggagattaa gttcggttta cgtttatttt agtactaatt gtaacgtgag
     2881 actacgtatc gggaatcgcc taattaaagc attaatgcga acctgattag attcaccgac
     2941 cctcctatcg tgtcgacctt tctgtttctt agaatttttt ggtagtctat gtactaataa
     3001 tgtcagcttc gtatttattt cataagcaat ttgcatttgc aatttgtttt ttacttttat
     3061 ttttattgta ttgtggaatg tggactcgta ccaacatgaa gttatatacc accaaaaaaa
     3121 ttacagttag tcaaaagatt cacgagtgag agctacttat gattgtcttt tacgtatatg
     3181 tctaattgtc tatttgctca ataatctttg tactttcttt tgtcgttgat aaaatcacaa
     3241 agttccaaaa gtaatcgaat gatttgcttt taagaaaaga agagctcaat aattcaacat
     3301 atatctgtac acaattaccg cctttgagtg agctctagat gccacctgac gtctaagaag
     3361 aattcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     3421 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     3481 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     3541 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg ctagagcagc
     3601 ttgccaacat ggtggagcac gacactctcg tctactccaa gaatatcaaa gatacagtct
     3661 cagaagacca aagggctatt gagacttttc aacaaagggt aatatcggga aacctcctcg
     3721 gattccattg cccagctatc tgtcacttca tcaaaaggac agtagaaaag gaaggtggca
     3781 cctacaaatg ccatcattgc gataaaggaa aggctatcgt tcaagatgcc tctgccgaca
     3841 gtggtcccaa agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa
     3901 ccacgtcttc aaagcaagtg gattgatgtg aacatggtgg agcacgacac tctcgtctac
     3961 tccaagaata tcaaagatac agtctcagaa gaccaaaggg ctattgagac ttttcaacaa
     4021 agggtaatat cgggaaacct cctcggattc cattgcccag ctatctgtca cttcatcaaa
     4081 aggacagtag aaaaggaagg tggcacctac aaatgccatc attgcgataa aggaaaggct
     4141 atcgttcaag atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc
     4201 atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgatatc
     4261 tccactgacg taagggatga cgcacaatcc cactatcctt cgcaagaccc ttcctctata
     4321 taaggaagtt catttcattt ggagaggaca cgctgaaatc accagtctct ctctacaaat
     4381 ctatctctct cgagctttcg cagatcccgg ggggcaatga gatatgggga ttgaacaaga
     4441 tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc
     4501 acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc
     4561 ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactccagg acgaggcagc
     4621 gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac
     4681 tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc
     4741 tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac
     4801 gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg
     4861 tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct
     4921 cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt
     4981 cgtgacacat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg
     5041 attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac
     5101 ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg
     5161 tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg
     5221 aagtagatgc cgaccggatc tgtcgatcga caagctcgag tttctccata ataatgtgtg
     5281 agtagttccc agataaggga attagggttc ctatagggtt tcgctcatgt gttgagcata
     5341 taagaaaccc ttagtatgta tttgtatttg taaaatactt ctatcaataa aatttctaat
     5401 tcctaaaacc aaaatccagt actaaaatcc agatcccccg aattaattcg gcgttaattc
     5461 agtacattaa aaacgtccgc aatgtgttat taagttgtct aagcgtcaat ttgtttacac
     5521 cacaatatat cctgccacca gccagccaac agctccccga ccggcagctc ggcacaaaat
     5581 caccactcga tacaggcagc ccatcagtcc gggacggcgt cagcgggaga gccgttgtaa
     5641 ggcggcagac tttgctcatg ttaccgatgc tattcggaag aacggcaact aagctgccgg
     5701 gtttgaaaca cggatgatct cgcggagggt agcatgttga ttgtaacgat gacagagcgt
     5761 tgctgcctgt gatcaccgcg gtttcaaaat cggctccgtc gatactatgt tatacgccaa
     5821 ctttgaaaac aactttgaaa aagctgtttt ctggtattta aggttttaga atgcaaggaa
     5881 cagtgaattg gagttcgtct tgttataatt agcttcttgg ggtatcttta aatactgtag
     5941 aaaagaggaa ggaaataata aatggctaaa atgagaatat caccggaatt gaaaaaactg
     6001 atcgaaaaat accgctgcgt aaaagatacg gaaggaatgt ctcctgctaa ggtatataag
     6061 ctggtgggag aaaatgaaaa cctatattta aaaatgacgg acagccggta taaagggacc
     6121 acctatgatg tggaacggga aaaggacatg atgctatggc tggaaggaaa gctgcctgtt
     6181 ccaaaggtcc tgcactttga acggcatgat ggctggagca atctgctcat gagtgaggcc
     6241 gatggcgtcc tttgctcgga agagtatgaa gatgaacaaa gccctgaaaa gattatcgag
     6301 ctgtatgcgg agtgcatcag gctctttcac tccatcgaca tatcggattg tccctatacg
     6361 aatagcttag acagccgctt agccgaattg gattacttac tgaataacga tctggccgat
     6421 gtggattgcg aaaactggga agaagacact ccatttaaag atccgcgcga gctgtatgat
     6481 tttttaaaga cggaaaagcc cgaagaggaa cttgtctttt cccacggcga cctgggagac
     6541 agcaacatct ttgtgaaaga tggcaaagta agtggcttta ttgatcttgg gagaagcggc
     6601 agggcggaca agtggtatga cattgccttc tgcgtccggt cgatcaggga ggatatcggg
     6661 gaagaacagt atgtcgagct attttttgac ttactgggga tcaagcctga ttgggagaaa
     6721 ataaaatatt atattttact ggatgaattg ttttagtacc tagaatgcat gaccaaaatc
     6781 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     6841 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     6901 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     6961 ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac
     7021 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     7081 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     7141 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     7201 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     7261 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     7321 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     7381 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     7441 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     7501 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     7561 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg
     7621 atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatatg gtgcactctc
     7681 agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg
     7741 actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
     7801 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
     7861 agaggttttc accgtcatca ccgaaacgcg cgaggcaggg tgccttgatg tgggcgccgg
     7921 cggtcgagtg gcgacggcgc ggcttgtccg cgccctggta gattgcctgg ccgtaggcca
     7981 gccatttttg agcggccagc ggccgcgata ggccgacgcg aagcggcggg gcgtagggag
     8041 cgcagcgacc gaagggtagg cgctttttgc agctcttcgg ctgtgcgctg gccagacagt
     8101 tatgcacagg ccaggcgggt tttaagagtt ttaataagtt ttaaagagtt ttaggcggaa
     8161 aaatcgcctt ttttctcttt tatatcagtc acttacatgt gtgaccggtt cccaatgtac
     8221 ggctttgggt tcccaatgta cgggttccgg ttcccaatgt acggctttgg gttcccaatg
     8281 tacgtgctat ccacaggaaa gagacctttt cgaccttttt cccctgctag ggcaatttgc
     8341 cctagcatct gctccgtaca ttaggaaccg gcggatgctt cgccctcgat caggttgcgg
     8401 tagcgcatga ctaggatcgg gccagcctgc cccgcctcct ccttcaaatc gtactccggc
     8461 aggtcatttg acccgatcag cttgcgcacg gtgaaacaga acttcttgaa ctctccggcg
     8521 ctgccactgc gttcgtagat cgtcttgaac aaccatctgg cttctgcctt gcctgcggcg
     8581 cggcgtgcca ggcggtagag aaaacggccg atgccgggat cgatcaaaaa gtaatcgggg
     8641 tgaaccgtca gcacgtccgg gttcttgcct tctgtgatct cgcggtacat ccaatcagct
     8701 agctcgatct cgatgtactc cggccgcccg gtttcgctct ttacgatctt gtagcggcta
     8761 atcaaggctt caccctcgga taccgtcacc aggcggccgt tcttggcctt cttcgtacgc
     8821 tgcatggcaa cgtgcgtggt gtttaaccga atgcaggttt ctaccaggtc gtctttctgc
     8881 tttccgccat cggctcgccg gcagaacttg agtacgtccg caacgtgtgg acggaacacg
     8941 cggccgggct tgtctccctt cccttcccgg tatcggttca tggattcggt tagatgggaa
     9001 accgccatca gtaccaggtc gtaatcccac acactggcca tgccggccgg ccctgcggaa
     9061 acctctacgt gcccgtctgg aagctcgtag cggatcacct cgccagctcg tcggtcacgc
     9121 ttcgacagac ggaaaacggc cacgtccatg atgctgcgac tatcgcgggt gcccacgtca
     9181 tagagcatcg gaacgaaaaa atctggttgc tcgtcgccct tgggcggctt cctaatcgac
     9241 ggcgcaccgg ctgccggcgg ttgccgggat tctttgcgga ttcgatcagc ggccgcttgc
     9301 cacgattcac cggggcgtgc ttctgcctcg atgcgttgcc gctgggcggc ctgcgcggcc
     9361 ttcaacttct ccaccaggtc atcacccagc gccgcgccga tttgtaccgg gccggatggt
     9421 ttgcgaccgc tcacgccgat tcctcgggct tgggggttcc agtgccattg cagggccggc
     9481 aggcaaccca gccgcttacg cctggccaac cgcccgttcc tccacacatg gggcattcca
     9541 cggcgtcggt gcctggttgt tcttgatttt ccatgccgcc tcctttagcc gctaaaattc
     9601 atctactcat ttattcattt gctcatttac tctggtagct gcgcgatgta ttcagatagc
     9661 agctcggtaa tggtcttgcc ttggcgtacc gcgtacatct tcagcttggt gtgatcctcc
     9721 gccggcaact gaaagttgac ccgcttcatg gctggcgtgt ctgccaggct ggccaacgtt
     9781 gcagccttgc tgctgcgtgc gctcggacgg ccggcactta gcgtgtttgt gcttttgctc
     9841 attttctctt tacctcatta actcaaatga gttttgattt aatttcagcg gccagcgcct
     9901 ggacctcgcg ggcagcgtcg ccctcgggtt ctgattcaag aacggttgtg ccggcggcgg
     9961 cagtgcctgg gtagctcacg cgctgcgtga tacgggactc aagaatgggc agctcgtacc
    10021 cggccagcgc ctcggcaacc tcaccgccga tgcgcgtgcc tttgatcgcc cgcgacacga
    10081 caaaggccgc ttgtagcctt ccatccgtga cctcaatgcg ctgcttaacc agctccacca
    10141 ggtcggcggt ggcccatatg tcgtaagggc ttggctgcac cggaatcagc acgaagtcgg
    10201 ctgccttgat cgcggacaca gccaagtccg ccgcctgggg cgctccgtcg atcactacga
    10261 agtcgcgccg gccgatggcc ttcacgtcgc ggtcaatcgt cgggcggtcg atgccgacaa
    10321 cggttagcgg ttgatcttcc cgcacggccg cccaatcgcg ggcactgccc tggggatcgg
    10381 aatcgactaa cagaacatcg gccccggcga gttgcagggc gcgggctaga tgggttgcga
    10441 tggtcgtctt gcctgacccg cctttctggt taagtacagc gataaccttc atgcgttccc
    10501 cttgcgtatt tgtttattta ctcatcgcat catatacgca gcgaccgcat gacgcaagct
    10561 gttttactca aatacacatc acctttttag acggcggcgc tcggtttctt cagcggccaa
    10621 gctggccggc caggccgcca gcttggcatc agacaaaccg gccaggattt catgcagccg
    10681 cacggttgag acgtgcgcgg gcggctcgaa cacgtacccg gccgcgatca tctccgcctc
    10741 gatctcttcg gtaatgaaaa acggttcgtc ctggccgtcc tggtgcggtt tcatgcttgt
    10801 tcctcttggc gttcattctc ggcggccgcc agggcgtcgg cctcggtcaa tgcgtcctca
    10861 cggaaggcac cgcgccgcct ggcctcggtg ggcgtcactt cctcgctgcg ctcaagtgcg
    10921 cggtacaggg tcgagcgatg cacgccaagc agtgcagccg cctctttcac ggtgcggcct
    10981 tcctggtcga tcagctcgcg ggcgtgcgcg atctgtgccg gggtgagggt agggcggggg
    11041 ccaaacttca cgcctcgggc cttggcggcc tcgcgcccgc tccgggtgcg gtcgatgatt
    11101 agggaacgct cgaactcggc aatgccggcg aacacggtca acaccatgcg gccggccggc
    11161 gtggtggtgt cggcccacgg ctctgccagg ctacgcaggc ccgcgccggc ctcctggatg
    11221 cgctcggcaa tgtccagtag gtcgcgggtg ctgcgggcca ggcggtctag cctggtcact
    11281 gtcacaacgt cgccagggcg taggtggtca agcatcctgg ccagctccgg gcggtcgcgc
    11341 ctggtgccgg tgatcttctc ggaaaacagc ttggtgcagc cggccgcgtg cagttcggcc
    11401 cgttggttgg tcaagtcctg gtcgtcggtg ctgacgcggg catagcccag caggccagcg
    11461 gcggcgctct tgttcatggc gtaatgtctc cggttctagt cgcaagtatt ctactttatg
    11521 cgactaaaac acgcgacaag aaaacgccag gaaaagggca gggcggcagc ctgtcgcgta
    11581 acttaggact tgtgcgacat gtcgttttca gaagacggct gcactgaacg tcagaagccg
    11641 actgcactat agcagcggag gggttggatc aaagtacttt gatcccgagg ggaaccctgt
    11701 ggttggcatg cacatacaaa tggacgaacg gataaacctt ttcacgccct tttaaatatc
    11761 cgattattct aataaacgct cttttctctt aggtttaccc gccaatatat cctgtcaaac
    11821 actgatagtt taattcccga tctagtaaca tagatgacac cgcgcgcgat aatttatcct
    11881 agtttgcgcg ctatattttg ttttctatcg cgtattaaat gtataattgc gggactctaa
    11941 tcataaaaac ccatctcata aataacgtca tgcattacat gttaattatt acatgcttaa
    12001 cgtaattcaa cagaaattat atgataatca tcgcaagacc ggcaacagga ttcaatctta
    12061 agaaacttta ttgccaaatg tttgaacgat cggggaaatt cgagctggtc accaattcac
    12121 acgtgatggt gatggtgatg gctagcgttc ttgtagccga aatctggaat gttggtccag
    12181 cgctcgcgaa agacgtgcgc ggcgagcttc ggcttgcggt cacgagtgaa cacgcccttc
    12241 ttgtttcctt ggacgcgcat cacgccctga gaggtcgcga agtccgcgaa gttccacgct
    12301 tgctcaccca cgaagttctc aaactcatcg aacacgacgt ggttcgcctg gtagtactcg
    12361 acttgatatt cctcggtgaa catcactgga tcaatgtcgt gaaagcccgc aacggtgtct
    12421 gcgccgtact cagtgatcat gatcggcttt cctgggcaac gcttgttcca cgcgtgaaat
    12481 tcctggcgga gatggacttt ggccgcttcg agatcaccgc catcgaagta ccatccgtta
    12541 tagcgattga gcgcgatgac gtcaatcagt tcggcgactt tgtccgtctc cggggtagcc
    12601 atcacaaaca gcacgatcgt gaccggacgc ttctgtgggt cgagttcctt ggtcagctcc
    12661 accaacggct tgaagtactc gtacgcgccc tcttcctcag tcgccgcctc gttggcgatg
    12721 ctccacatca cgacgcttgg atggttcttg tcacgagaca ccagttcacg gagaacgtct
    12781 tgatggtgct caaacgtccg aatcttctcc caggtactga cgcgctcgct gccttcgccg
    12841 agtcccgtgg tggccatgaa gttgaggtgc acgccaactg ccggagtctc gtcgatcacg
    12901 accagaccct cgcgatccgc aagacgcatc aactcttcag agtacggata gtgtgcggtc
    12961 cggaagctgt tggcgccgat ccatttgagg atattgaaat ccatcacatt gctcgcttcg
    13021 ttaaagccac ggccgttgat aggagtgtcc tcatgtttgc caaagccctt gaagtagaac
    13081 ggtttgttgt tgatgaggaa cttgccgtcg ttgacttcca cggtccgcac gccgaacggc
    13141 tcttcataga catcgatggt cagtccgtcg ttcaccagtt ccactttgat ctggtagaga
    13201 tacgtgttca gtggttccca gaggatgaca ttcggaatct ccacgttacc gctcaggccc
    13261 tcggtgcttg cgaccacttt gccttcctca tccacgaccg acactttcac ggtctcggct
    13321 ttgccttgaa agtccaccgt ataggtcaca gtcccggttg ggccattgaa gtcggtcaca
    13381 accgagatgt cctcgacgta cgtaaacggg gtcgtgtaga ttttcaccgg acggtgcagg
    13441 cctgcatagt tgaagaagtc gaagttcggc ttgttacgaa tgacttttcc gaggccctct
    13501 tcgtggcgct cgctgtacag ccccaccggg agggtgctat cgtcgaggat gttgtccacg
    13561 gcgacggtga cgcgattcat gccatcacgc agcgagttgt tgatttccgc ttcgaatggc
    13621 aggaatccgc ccttgtgctc cacgaccagc tcaccattga catagacaat tgctttgtga
    13681 gttgcagagc cgaagcggag cacgatacgc tgatccttca gataggccgg caccgtgaac
    13741 tcacgttcgt accagacata tccgatatgg ttgcggattt ccttggtcac gccaatgtca
    13801 ttgtaactgc ttgggacggc catactaata gtgtcggtca gcttgctttc gtaccacttc
    13861 tcttccagtc ctttcccgta gtccagcttg aagttccaga cgccattgag gtcgaagacg
    13921 ccacgggtct cggtgttgat cgggtacaga ctagttcgtc ggttctgtaa ctatcatcat
    13981 catcatagac acacgaaata aagtaatcag attatcagtt aaagctatgt aatatttaca
    14041 ccataaccaa tcaattaaaa aatagatcag tttaaagaaa gatcaaagct caaaaaaata
    14101 aaaagagaaa agggtcctaa ccaagaaaat gaaggagaaa aactagaaat ttaccctcag
    14161 atctaccatg
//
