LOCUS       sequence-477049-       14546 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression vector of deactivated ribozyme with IGS2 split GFPuv.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14546)
  AUTHORS   Liu Y, Rajput R, Islam MT, Valle ID, Yao T, Agrawal R, Boone BA, 
            Eckert CA, Abraham PE, Chen JG, Tuskan GA, Yang X
  TITLE     A split ribozyme system for in vivo plant RNA imaging and genetic 
            engineering.
  JOURNAL   Plant Biotechnol J. 2025 May;23(5):1640-1649. doi: 
            10.1111/pbi.14612. Epub 2025 Feb 7.
  PUBMED    39919021
REFERENCE   2  (bases 1 to 14546)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/pbi.14612";
            journalName: "Plant Biotechnol J"; date: "2025-05"; volume: "23"; 
            issue: "5"; pages: "1640-1649"
COMMENT     Sequence Label: pXYB177
FEATURES             Location/Qualifiers
     source          1..14546
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(16..361)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     723..745
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     737..754
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     738..754
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1269..1614
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     terminator      2813..3061
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     primer_bind     complement(3751..3767)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3751..3767)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3764..3786)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3775..3791
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3799..3805,3806..3823,3824..3829))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3844..3865
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        4056..4732
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     CDS             4800..5597
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLL
                     SSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEH
                     QGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDI
                     ALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4857..4876)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5467..5486
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5637..5811
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    5889..5913
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6338..7132
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      7219..7807
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7708..7727
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7961..7978
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7993..8133
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8059..8078)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8219..8241
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8477..8671
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8737..9810)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10239..10868)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    12169..12193
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     terminator      complement(12215..12467)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(12499..12516)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(join(12523..14340,14531..14545))
                     /codon_start=1
                     /product="beta-glucuronidase"
                     /label=GUSPlus(TM)
                     /note="codon-optimized Staphylococcus gusA gene with a 
                     catalase intron to ensure expression in plants but not 
                     bacteria"
                     /translation="MVDLRNRRTSLYPINTETRGVFDLNGVWNFKLDYGKGLEEKWYES
                     KLTDTISMAVPSSYNDIGVTKEIRNHIGYVWYEREFTVPAYLKDQRIVLRFGSATHKAI
                     VYVNGELVVEHKGGFLPFEAEINNSLRDGMNRVTVAVDNILDDSTLPVGLYSERHEEGL
                     GKVIRNKPNFDFFNYAGLHRPVKIYTTPFTYVEDISVVTDFNGPTGTVTYTVDFQGKAE
                     TVKVSVVDEEGKVVASTEGLSGNVEIPNVILWEPLNTYLYQIKVELVNDGLTIDVYEEP
                     FGVRTVEVNDGKFLINNKPFYFKGFGKHEDTPINGRGFNEASNVMDFNILKWIGANSFR
                     TAHYPYSEELMRLADREGLVVIDETPAVGVHLNFMATTGLGEGSERVSTWEKIRTFEHH
                     QDVLRELVSRDKNHPSVVMWSIANEAATEEEGAYEYFKPLVELTKELDPQKRPVTIVLF
                     VMATPETDKVAELIDVIALNRYNGWYFDGGDLEAAKVHLRQEFHAWNKRCPGKPIMITE
                     YGADTVAGFHDIDPVMFTEEYQVEYYQANHVVFDEFENFVGEQAWNFADFATSQGVMRV
                     QGNKKGVFTRDRKPKLAAHVFRERWTNIPDFGYKN"
     intron          14341..14530
                     /label=cat1 intron
                     /note="castor bean catalase intron, modified"
ORIGIN
        1 gtcaagagtc ccccgtgttc tctccaaatg aaatgaactt ccttatatag aggaagggtc
       61 ttgcgaagga tagtgggatt gtgcgtcatc ccttacgtca gtggagatat cacatcaatc
      121 cacttgcttt gaagacgtgg ttggaacgtc ttctttttcc acgatgctcc tcgtgggtgg
      181 gggtccatct ttgggaccac tgtcggcaga ggcatcttca acgatggcct ttcctttatc
      241 gcaatgatgg catttgtagg agccaccttc cttttccact atcttcacaa taaagtgaca
      301 gatagctggg caatggaatc cgaggaggtt tccggatatt accctttgtt gaaaagtctc
      361 aattgccctt tggtcttctg agactgtatc tttgatattt ttggagtaga caagtgtgtc
      421 gtgctccacc atgttgacga agattttctt cttgtcattg agtcgtaaga gactctgtat
      481 gaactgttcg ccagtcttta cggcgagttc tgttaggtcc tctatttgaa tctttgactc
      541 catgaagcta aactgaaggc gggaaacgac aatctgatcc aagctcaagc tgctctagca
      601 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
      661 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt
      721 ttcccagtca cgacgttgta aaacgacggc cagtgccaag cttgcatgcc tgcaggtccc
      781 cagattagcc ttttcaattt cagaaagaat gctaacccac agatggttag agaggcttac
      841 gcagcaggtc tcatcaagac gatctacccg agcaataatc tccaggaaat caaatacctt
      901 cccaagaagg ttaaagatgc agtcaaaaga ttcaggacta actgcatcaa gaacacagag
      961 aaagatatat ttctcaagat cagaagtact attccagtat ggacgattca aggcttgctt
     1021 cacaaaccaa ggcaagtaat agagattgga gtctctaaaa aggtagttcc cactgaatca
     1081 aaggccatgg agtcaaagat tcaaatagag gacctaacag aactcgccgt aaagactggc
     1141 gaacagttca tacagagtct cttacgactc aatgacaaga agaaaatctt cgtcaacatg
     1201 gtggagcacg acacacttgt ctactccaaa aatatcaaag atacagtctc agaagaccaa
     1261 agggcaattg agacttttca acaaagggta atatccggaa acctcctcgg attccattgc
     1321 ccagctatct gtcactttat tgtgaagata gtggaaaagg aaggtggctc ctacaaatgc
     1381 catcattgcg ataaaggaaa ggccatcgtt gaagatgcct ctgccgacag tggtcccaaa
     1441 gatggacccc cacccacgag gagcatcgtg gaaaaagaag acgttccaac cacgtcttca
     1501 aagcaagtgg attgatgtga tatctccact gacgtaaggg atgacgcaca atcccactat
     1561 ccttcgcaag acccttcctc tatataagga agttcatttc atttggagag aacacggggg
     1621 actcaaacat tactcattca caaaaccatc ttaaagcaac tacacaagtc ttgaaatttt
     1681 ctcatatttt ctatttacta tataaacttt taatcaaatc aagattaaag ttaattaaat
     1741 gacaaccttc aaaatcgagt cccggatcca cggcaacctc aacggggaga agttcgagtt
     1801 ggttggaggt ggagtaggtg aggagggtcg cctcgagatt gagatgaaga ctaaagataa
     1861 accactggca ttctctccct tcctgctgac cacttgcatg ggttacgggt tctaccactt
     1921 cgccagcttc ccaaagggga ttaagaacat ctataaatag caatatttac ctttgtagat
     1981 aaaagttatc aggcatgcac ctggtagcta gtctttaaac caatagattg catcggttta
     2041 aaaggcaaga ccgtcaaatt gcgggaaagg ggtcaacagc cgttcagtac caagtctcag
     2101 gggaaacttt gagatggcct tgcaaagggt atggtaataa gctgacggac atggtcctaa
     2161 ccacgcagcc aagtcctaag tcaacagatc ttctgttgat atggatgcag ttcacaaact
     2221 aaatgtcggt cggggaagat gtattcttct cataagatat agtcggacct ctccttaatg
     2281 ggagctagcg gatgaagtga tgcaacactg gagccgctgg gaactaattt gtatgcgaaa
     2341 gtatattgat tagttttgga gtactcgctt catgctgcaa cgaacggagg ttacaccaac
     2401 accaggaagg agatctatga agacggcggc atcttggagg tcaacttccg ttacacttac
     2461 gagttcaaca agatcatcgg tgacgtcgag tgcattggac atggattccc aagtcagagt
     2521 ccgatcttca aggacacgat cgtgaagagt tgtcccacgg tggacctgat gttgccaatg
     2581 tccgggaaca tcatcgccag ctcctacgct tacgccttcc aactgaagga cggctctttc
     2641 tacacggcag aagtcaagaa caacatagac ttcaagaatc caatccacga gtccttctcg
     2701 aagtcagggc ccatgttcac ccacagacgt gtcgaggaga ctctcaccaa ggagaacctt
     2761 gccatagtgg agtaccagca ggttttcaac agcgccccaa gagacatgta gatatgaaga
     2821 tgaagatgaa atatttggtg tgtcaaataa aaagcttgtg tgcttaagtt tgtgtttttt
     2881 tcttggcttg ttgtgttatg aatttgtggc tttttctaat attaaatgaa tgtaagatct
     2941 cattataatg aataaacaaa tgtttctata atccattgtg aatgttttgt tggatctctt
     3001 ctgcagcata taactactgt atgtgctatg gtatggacta tggaatatga ttaaagataa
     3061 gatgggctca tagagtaaaa cgaggcgagg gacctataaa cctcccttca tcatgctatt
     3121 tcatgatcta ttttataaaa taaagatgta gaaaaaagta agcgtaataa ccgcaaaaca
     3181 aatgatttaa aacatggcac ataatgagga gattaagttc ggtttacgtt tattttagta
     3241 ctaattgtaa cgtgagacta cgtatcggga atcgcctaat taaagcatta atgcgaacct
     3301 gattagattc accgaccctc ctatcgtgtc gacctttctg tttcttagaa ttttttggta
     3361 gtctatgtac taataatgtc agcttcgtat ttatttcata agcaatttgc atttgcaatt
     3421 tgttttttac ttttattttt attgtattgt ggaatgtgga ctcgtaccaa catgaagtta
     3481 tataccacca aaaaaattac agttagtcaa aagattcacg agtgagagct acttatgatt
     3541 gtcttttacg tatatgtcta attgtctatt tgctcaataa tctttgtact ttcttttgtc
     3601 gttgataaaa tcacaaagtt ccaaaagtaa tcgaatgatt tgcttttaag aaaagaagag
     3661 ctcaataatt caacatatat ctgtacacaa ttaccgcctt tgagtgagct ctagatgcca
     3721 cctgacgtct aagaagaatt cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     3781 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     3841 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     3901 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     3961 tattggctag agcagcttgc caacatggtg gagcacgaca ctctcgtcta ctccaagaat
     4021 atcaaagata cagtctcaga agaccaaagg gctattgaga cttttcaaca aagggtaata
     4081 tcgggaaacc tcctcggatt ccattgccca gctatctgtc acttcatcaa aaggacagta
     4141 gaaaaggaag gtggcaccta caaatgccat cattgcgata aaggaaaggc tatcgttcaa
     4201 gatgcctctg ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa
     4261 aaagaagacg ttccaaccac gtcttcaaag caagtggatt gatgtgaaca tggtggagca
     4321 cgacactctc gtctactcca agaatatcaa agatacagtc tcagaagacc aaagggctat
     4381 tgagactttt caacaaaggg taatatcggg aaacctcctc ggattccatt gcccagctat
     4441 ctgtcacttc atcaaaagga cagtagaaaa ggaaggtggc acctacaaat gccatcattg
     4501 cgataaagga aaggctatcg ttcaagatgc ctctgccgac agtggtccca aagatggacc
     4561 cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt
     4621 ggattgatgt gatatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca
     4681 agacccttcc tctatataag gaagttcatt tcatttggag aggacacgct gaaatcacca
     4741 gtctctctct acaaatctat ctctctcgag ctttcgcaga tcccgggggg caatgagata
     4801 tggggattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat
     4861 tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt
     4921 cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac
     4981 tccaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg
     5041 tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc
     5101 aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa
     5161 tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc
     5221 gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg
     5281 aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg
     5341 acggcgagga tctcgtcgtg acacatggcg atgcctgctt gccgaatatc atggtggaaa
     5401 atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg
     5461 acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct
     5521 tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc
     5581 ttgacgagtt cttctgaagt agatgccgac cggatctgtc gatcgacaag ctcgagtttc
     5641 tccataataa tgtgtgagta gttcccagat aagggaatta gggttcctat agggtttcgc
     5701 tcatgtgttg agcatataag aaacccttag tatgtatttg tatttgtaaa atacttctat
     5761 caataaaatt tctaattcct aaaaccaaaa tccagtacta aaatccagat cccccgaatt
     5821 aattcggcgt taattcagta cattaaaaac gtccgcaatg tgttattaag ttgtctaagc
     5881 gtcaatttgt ttacaccaca atatatcctg ccaccagcca gccaacagct ccccgaccgg
     5941 cagctcggca caaaatcacc actcgataca ggcagcccat cagtccggga cggcgtcagc
     6001 gggagagccg ttgtaaggcg gcagactttg ctcatgttac cgatgctatt cggaagaacg
     6061 gcaactaagc tgccgggttt gaaacacgga tgatctcgcg gagggtagca tgttgattgt
     6121 aacgatgaca gagcgttgct gcctgtgatc accgcggttt caaaatcggc tccgtcgata
     6181 ctatgttata cgccaacttt gaaaacaact ttgaaaaagc tgttttctgg tatttaaggt
     6241 tttagaatgc aaggaacagt gaattggagt tcgtcttgtt ataattagct tcttggggta
     6301 tctttaaata ctgtagaaaa gaggaaggaa ataataaatg gctaaaatga gaatatcacc
     6361 ggaattgaaa aaactgatcg aaaaataccg ctgcgtaaaa gatacggaag gaatgtctcc
     6421 tgctaaggta tataagctgg tgggagaaaa tgaaaaccta tatttaaaaa tgacggacag
     6481 ccggtataaa gggaccacct atgatgtgga acgggaaaag gacatgatgc tatggctgga
     6541 aggaaagctg cctgttccaa aggtcctgca ctttgaacgg catgatggct ggagcaatct
     6601 gctcatgagt gaggccgatg gcgtcctttg ctcggaagag tatgaagatg aacaaagccc
     6661 tgaaaagatt atcgagctgt atgcggagtg catcaggctc tttcactcca tcgacatatc
     6721 ggattgtccc tatacgaata gcttagacag ccgcttagcc gaattggatt acttactgaa
     6781 taacgatctg gccgatgtgg attgcgaaaa ctgggaagaa gacactccat ttaaagatcc
     6841 gcgcgagctg tatgattttt taaagacgga aaagcccgaa gaggaacttg tcttttccca
     6901 cggcgacctg ggagacagca acatctttgt gaaagatggc aaagtaagtg gctttattga
     6961 tcttgggaga agcggcaggg cggacaagtg gtatgacatt gccttctgcg tccggtcgat
     7021 cagggaggat atcggggaag aacagtatgt cgagctattt tttgacttac tggggatcaa
     7081 gcctgattgg gagaaaataa aatattatat tttactggat gaattgtttt agtacctaga
     7141 atgcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     7201 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     7261 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     7321 tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc
     7381 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     7441 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     7501 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     7561 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     7621 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     7681 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     7741 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     7801 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     7861 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     7921 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
     7981 agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg
     8041 catatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact
     8101 ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac
     8161 gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc
     8221 gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagggtgcc
     8281 ttgatgtggg cgccggcggt cgagtggcga cggcgcggct tgtccgcgcc ctggtagatt
     8341 gcctggccgt aggccagcca tttttgagcg gccagcggcc gcgataggcc gacgcgaagc
     8401 ggcggggcgt agggagcgca gcgaccgaag ggtaggcgct ttttgcagct cttcggctgt
     8461 gcgctggcca gacagttatg cacaggccag gcgggtttta agagttttaa taagttttaa
     8521 agagttttag gcggaaaaat cgcctttttt ctcttttata tcagtcactt acatgtgtga
     8581 ccggttccca atgtacggct ttgggttccc aatgtacggg ttccggttcc caatgtacgg
     8641 ctttgggttc ccaatgtacg tgctatccac aggaaagaga ccttttcgac ctttttcccc
     8701 tgctagggca atttgcccta gcatctgctc cgtacattag gaaccggcgg atgcttcgcc
     8761 ctcgatcagg ttgcggtagc gcatgactag gatcgggcca gcctgccccg cctcctcctt
     8821 caaatcgtac tccggcaggt catttgaccc gatcagcttg cgcacggtga aacagaactt
     8881 cttgaactct ccggcgctgc cactgcgttc gtagatcgtc ttgaacaacc atctggcttc
     8941 tgccttgcct gcggcgcggc gtgccaggcg gtagagaaaa cggccgatgc cgggatcgat
     9001 caaaaagtaa tcggggtgaa ccgtcagcac gtccgggttc ttgccttctg tgatctcgcg
     9061 gtacatccaa tcagctagct cgatctcgat gtactccggc cgcccggttt cgctctttac
     9121 gatcttgtag cggctaatca aggcttcacc ctcggatacc gtcaccaggc ggccgttctt
     9181 ggccttcttc gtacgctgca tggcaacgtg cgtggtgttt aaccgaatgc aggtttctac
     9241 caggtcgtct ttctgctttc cgccatcggc tcgccggcag aacttgagta cgtccgcaac
     9301 gtgtggacgg aacacgcggc cgggcttgtc tcccttccct tcccggtatc ggttcatgga
     9361 ttcggttaga tgggaaaccg ccatcagtac caggtcgtaa tcccacacac tggccatgcc
     9421 ggccggccct gcggaaacct ctacgtgccc gtctggaagc tcgtagcgga tcacctcgcc
     9481 agctcgtcgg tcacgcttcg acagacggaa aacggccacg tccatgatgc tgcgactatc
     9541 gcgggtgccc acgtcataga gcatcggaac gaaaaaatct ggttgctcgt cgcccttggg
     9601 cggcttccta atcgacggcg caccggctgc cggcggttgc cgggattctt tgcggattcg
     9661 atcagcggcc gcttgccacg attcaccggg gcgtgcttct gcctcgatgc gttgccgctg
     9721 ggcggcctgc gcggccttca acttctccac caggtcatca cccagcgccg cgccgatttg
     9781 taccgggccg gatggtttgc gaccgctcac gccgattcct cgggcttggg ggttccagtg
     9841 ccattgcagg gccggcaggc aacccagccg cttacgcctg gccaaccgcc cgttcctcca
     9901 cacatggggc attccacggc gtcggtgcct ggttgttctt gattttccat gccgcctcct
     9961 ttagccgcta aaattcatct actcatttat tcatttgctc atttactctg gtagctgcgc
    10021 gatgtattca gatagcagct cggtaatggt cttgccttgg cgtaccgcgt acatcttcag
    10081 cttggtgtga tcctccgccg gcaactgaaa gttgacccgc ttcatggctg gcgtgtctgc
    10141 caggctggcc aacgttgcag ccttgctgct gcgtgcgctc ggacggccgg cacttagcgt
    10201 gtttgtgctt ttgctcattt tctctttacc tcattaactc aaatgagttt tgatttaatt
    10261 tcagcggcca gcgcctggac ctcgcgggca gcgtcgccct cgggttctga ttcaagaacg
    10321 gttgtgccgg cggcggcagt gcctgggtag ctcacgcgct gcgtgatacg ggactcaaga
    10381 atgggcagct cgtacccggc cagcgcctcg gcaacctcac cgccgatgcg cgtgcctttg
    10441 atcgcccgcg acacgacaaa ggccgcttgt agccttccat ccgtgacctc aatgcgctgc
    10501 ttaaccagct ccaccaggtc ggcggtggcc catatgtcgt aagggcttgg ctgcaccgga
    10561 atcagcacga agtcggctgc cttgatcgcg gacacagcca agtccgccgc ctggggcgct
    10621 ccgtcgatca ctacgaagtc gcgccggccg atggccttca cgtcgcggtc aatcgtcggg
    10681 cggtcgatgc cgacaacggt tagcggttga tcttcccgca cggccgccca atcgcgggca
    10741 ctgccctggg gatcggaatc gactaacaga acatcggccc cggcgagttg cagggcgcgg
    10801 gctagatggg ttgcgatggt cgtcttgcct gacccgcctt tctggttaag tacagcgata
    10861 accttcatgc gttccccttg cgtatttgtt tatttactca tcgcatcata tacgcagcga
    10921 ccgcatgacg caagctgttt tactcaaata cacatcacct ttttagacgg cggcgctcgg
    10981 tttcttcagc ggccaagctg gccggccagg ccgccagctt ggcatcagac aaaccggcca
    11041 ggatttcatg cagccgcacg gttgagacgt gcgcgggcgg ctcgaacacg tacccggccg
    11101 cgatcatctc cgcctcgatc tcttcggtaa tgaaaaacgg ttcgtcctgg ccgtcctggt
    11161 gcggtttcat gcttgttcct cttggcgttc attctcggcg gccgccaggg cgtcggcctc
    11221 ggtcaatgcg tcctcacgga aggcaccgcg ccgcctggcc tcggtgggcg tcacttcctc
    11281 gctgcgctca agtgcgcggt acagggtcga gcgatgcacg ccaagcagtg cagccgcctc
    11341 tttcacggtg cggccttcct ggtcgatcag ctcgcgggcg tgcgcgatct gtgccggggt
    11401 gagggtaggg cgggggccaa acttcacgcc tcgggccttg gcggcctcgc gcccgctccg
    11461 ggtgcggtcg atgattaggg aacgctcgaa ctcggcaatg ccggcgaaca cggtcaacac
    11521 catgcggccg gccggcgtgg tggtgtcggc ccacggctct gccaggctac gcaggcccgc
    11581 gccggcctcc tggatgcgct cggcaatgtc cagtaggtcg cgggtgctgc gggccaggcg
    11641 gtctagcctg gtcactgtca caacgtcgcc agggcgtagg tggtcaagca tcctggccag
    11701 ctccgggcgg tcgcgcctgg tgccggtgat cttctcggaa aacagcttgg tgcagccggc
    11761 cgcgtgcagt tcggcccgtt ggttggtcaa gtcctggtcg tcggtgctga cgcgggcata
    11821 gcccagcagg ccagcggcgg cgctcttgtt catggcgtaa tgtctccggt tctagtcgca
    11881 agtattctac tttatgcgac taaaacacgc gacaagaaaa cgccaggaaa agggcagggc
    11941 ggcagcctgt cgcgtaactt aggacttgtg cgacatgtcg ttttcagaag acggctgcac
    12001 tgaacgtcag aagccgactg cactatagca gcggaggggt tggatcaaag tactttgatc
    12061 ccgaggggaa ccctgtggtt ggcatgcaca tacaaatgga cgaacggata aaccttttca
    12121 cgccctttta aatatccgat tattctaata aacgctcttt tctcttaggt ttacccgcca
    12181 atatatcctg tcaaacactg atagtttaat tcccgatcta gtaacataga tgacaccgcg
    12241 cgcgataatt tatcctagtt tgcgcgctat attttgtttt ctatcgcgta ttaaatgtat
    12301 aattgcggga ctctaatcat aaaaacccat ctcataaata acgtcatgca ttacatgtta
    12361 attattacat gcttaacgta attcaacaga aattatatga taatcatcgc aagaccggca
    12421 acaggattca atcttaagaa actttattgc caaatgtttg aacgatcggg gaaattcgag
    12481 ctggtcacca attcacacgt gatggtgatg gtgatggcta gcgttcttgt agccgaaatc
    12541 tggaatgttg gtccagcgct cgcgaaagac gtgcgcggcg agcttcggct tgcggtcacg
    12601 agtgaacacg cccttcttgt ttccttggac gcgcatcacg ccctgagagg tcgcgaagtc
    12661 cgcgaagttc cacgcttgct cacccacgaa gttctcaaac tcatcgaaca cgacgtggtt
    12721 cgcctggtag tactcgactt gatattcctc ggtgaacatc actggatcaa tgtcgtgaaa
    12781 gcccgcaacg gtgtctgcgc cgtactcagt gatcatgatc ggctttcctg ggcaacgctt
    12841 gttccacgcg tgaaattcct ggcggagatg gactttggcc gcttcgagat caccgccatc
    12901 gaagtaccat ccgttatagc gattgagcgc gatgacgtca atcagttcgg cgactttgtc
    12961 cgtctccggg gtagccatca caaacagcac gatcgtgacc ggacgcttct gtgggtcgag
    13021 ttccttggtc agctccacca acggcttgaa gtactcgtac gcgccctctt cctcagtcgc
    13081 cgcctcgttg gcgatgctcc acatcacgac gcttggatgg ttcttgtcac gagacaccag
    13141 ttcacggaga acgtcttgat ggtgctcaaa cgtccgaatc ttctcccagg tactgacgcg
    13201 ctcgctgcct tcgccgagtc ccgtggtggc catgaagttg aggtgcacgc caactgccgg
    13261 agtctcgtcg atcacgacca gaccctcgcg atccgcaaga cgcatcaact cttcagagta
    13321 cggatagtgt gcggtccgga agctgttggc gccgatccat ttgaggatat tgaaatccat
    13381 cacattgctc gcttcgttaa agccacggcc gttgatagga gtgtcctcat gtttgccaaa
    13441 gcccttgaag tagaacggtt tgttgttgat gaggaacttg ccgtcgttga cttccacggt
    13501 ccgcacgccg aacggctctt catagacatc gatggtcagt ccgtcgttca ccagttccac
    13561 tttgatctgg tagagatacg tgttcagtgg ttcccagagg atgacattcg gaatctccac
    13621 gttaccgctc aggccctcgg tgcttgcgac cactttgcct tcctcatcca cgaccgacac
    13681 tttcacggtc tcggctttgc cttgaaagtc caccgtatag gtcacagtcc cggttgggcc
    13741 attgaagtcg gtcacaaccg agatgtcctc gacgtacgta aacggggtcg tgtagatttt
    13801 caccggacgg tgcaggcctg catagttgaa gaagtcgaag ttcggcttgt tacgaatgac
    13861 ttttccgagg ccctcttcgt ggcgctcgct gtacagcccc accgggaggg tgctatcgtc
    13921 gaggatgttg tccacggcga cggtgacgcg attcatgcca tcacgcagcg agttgttgat
    13981 ttccgcttcg aatggcagga atccgccctt gtgctccacg accagctcac cattgacata
    14041 gacaattgct ttgtgagttg cagagccgaa gcggagcacg atacgctgat ccttcagata
    14101 ggccggcacc gtgaactcac gttcgtacca gacatatccg atatggttgc ggatttcctt
    14161 ggtcacgcca atgtcattgt aactgcttgg gacggccata ctaatagtgt cggtcagctt
    14221 gctttcgtac cacttctctt ccagtccttt cccgtagtcc agcttgaagt tccagacgcc
    14281 attgaggtcg aagacgccac gggtctcggt gttgatcggg tacagactag ttcgtcggtt
    14341 ctgtaactat catcatcatc atagacacac gaaataaagt aatcagatta tcagttaaag
    14401 ctatgtaata tttacaccat aaccaatcaa ttaaaaaata gatcagttta aagaaagatc
    14461 aaagctcaaa aaaataaaaa gagaaaaggg tcctaaccaa gaaaatgaag gagaaaaact
    14521 agaaatttac cctcagatct accatg
//
