LOCUS       sequence-477051-       15300 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Deactivated ribozyme split sfGFP linked with GFPuv via T2A 
            expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15300)
  AUTHORS   Liu Y, Rajput R, Islam MT, Valle ID, Yao T, Agrawal R, Boone BA, 
            Eckert CA, Abraham PE, Chen JG, Tuskan GA, Yang X
  TITLE     A split ribozyme system for in vivo plant RNA imaging and genetic 
            engineering.
  JOURNAL   Plant Biotechnol J. 2025 May;23(5):1640-1649. doi: 
            10.1111/pbi.14612. Epub 2025 Feb 7.
  PUBMED    39919021
REFERENCE   2  (bases 1 to 15300)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/pbi.14612";
            journalName: "Plant Biotechnol J"; date: "2025-05"; volume: "23"; 
            issue: "5"; pages: "1640-1649"
COMMENT     Sequence Label: pXYB163
FEATURES             Location/Qualifiers
     source          1..15300
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(16..361)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     723..745
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     737..754
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     738..754
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1269..1614
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(1769..1797)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     2779..2799
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     CDS             2849..2905
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     terminator      3567..3815
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     primer_bind     complement(4505..4521)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4505..4521)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4518..4540)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4529..4545
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4553..4559,4560..4577,4578..4583))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4598..4619
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        4810..5486
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     CDS             5554..6351
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLL
                     SSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEH
                     QGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDI
                     ALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5611..5630)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6221..6240
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6391..6565
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    6643..6667
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             7092..7886
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      7973..8561
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8462..8481
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8715..8732
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8747..8887
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8813..8832)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8973..8995
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      9231..9425
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9491..10564)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10993..11622)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    12923..12947
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     terminator      complement(12969..13221)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(13253..13270)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(join(13277..15094,15285..15299))
                     /codon_start=1
                     /product="beta-glucuronidase"
                     /label=GUSPlus(TM)
                     /note="codon-optimized Staphylococcus gusA gene with a 
                     catalase intron to ensure expression in plants but not 
                     bacteria"
                     /translation="MVDLRNRRTSLYPINTETRGVFDLNGVWNFKLDYGKGLEEKWYES
                     KLTDTISMAVPSSYNDIGVTKEIRNHIGYVWYEREFTVPAYLKDQRIVLRFGSATHKAI
                     VYVNGELVVEHKGGFLPFEAEINNSLRDGMNRVTVAVDNILDDSTLPVGLYSERHEEGL
                     GKVIRNKPNFDFFNYAGLHRPVKIYTTPFTYVEDISVVTDFNGPTGTVTYTVDFQGKAE
                     TVKVSVVDEEGKVVASTEGLSGNVEIPNVILWEPLNTYLYQIKVELVNDGLTIDVYEEP
                     FGVRTVEVNDGKFLINNKPFYFKGFGKHEDTPINGRGFNEASNVMDFNILKWIGANSFR
                     TAHYPYSEELMRLADREGLVVIDETPAVGVHLNFMATTGLGEGSERVSTWEKIRTFEHH
                     QDVLRELVSRDKNHPSVVMWSIANEAATEEEGAYEYFKPLVELTKELDPQKRPVTIVLF
                     VMATPETDKVAELIDVIALNRYNGWYFDGGDLEAAKVHLRQEFHAWNKRCPGKPIMITE
                     YGADTVAGFHDIDPVMFTEEYQVEYYQANHVVFDEFENFVGEQAWNFADFATSQGVMRV
                     QGNKKGVFTRDRKPKLAAHVFRERWTNIPDFGYKN"
     intron          15095..15284
                     /label=cat1 intron
                     /note="castor bean catalase intron, modified"
ORIGIN
        1 gtcaagagtc ccccgtgttc tctccaaatg aaatgaactt ccttatatag aggaagggtc
       61 ttgcgaagga tagtgggatt gtgcgtcatc ccttacgtca gtggagatat cacatcaatc
      121 cacttgcttt gaagacgtgg ttggaacgtc ttctttttcc acgatgctcc tcgtgggtgg
      181 gggtccatct ttgggaccac tgtcggcaga ggcatcttca acgatggcct ttcctttatc
      241 gcaatgatgg catttgtagg agccaccttc cttttccact atcttcacaa taaagtgaca
      301 gatagctggg caatggaatc cgaggaggtt tccggatatt accctttgtt gaaaagtctc
      361 aattgccctt tggtcttctg agactgtatc tttgatattt ttggagtaga caagtgtgtc
      421 gtgctccacc atgttgacga agattttctt cttgtcattg agtcgtaaga gactctgtat
      481 gaactgttcg ccagtcttta cggcgagttc tgttaggtcc tctatttgaa tctttgactc
      541 catgaagcta aactgaaggc gggaaacgac aatctgatcc aagctcaagc tgctctagca
      601 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
      661 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt
      721 ttcccagtca cgacgttgta aaacgacggc cagtgccaag cttgcatgcc tgcaggtccc
      781 cagattagcc ttttcaattt cagaaagaat gctaacccac agatggttag agaggcttac
      841 gcagcaggtc tcatcaagac gatctacccg agcaataatc tccaggaaat caaatacctt
      901 cccaagaagg ttaaagatgc agtcaaaaga ttcaggacta actgcatcaa gaacacagag
      961 aaagatatat ttctcaagat cagaagtact attccagtat ggacgattca aggcttgctt
     1021 cacaaaccaa ggcaagtaat agagattgga gtctctaaaa aggtagttcc cactgaatca
     1081 aaggccatgg agtcaaagat tcaaatagag gacctaacag aactcgccgt aaagactggc
     1141 gaacagttca tacagagtct cttacgactc aatgacaaga agaaaatctt cgtcaacatg
     1201 gtggagcacg acacacttgt ctactccaaa aatatcaaag atacagtctc agaagaccaa
     1261 agggcaattg agacttttca acaaagggta atatccggaa acctcctcgg attccattgc
     1321 ccagctatct gtcactttat tgtgaagata gtggaaaagg aaggtggctc ctacaaatgc
     1381 catcattgcg ataaaggaaa ggccatcgtt gaagatgcct ctgccgacag tggtcccaaa
     1441 gatggacccc cacccacgag gagcatcgtg gaaaaagaag acgttccaac cacgtcttca
     1501 aagcaagtgg attgatgtga tatctccact gacgtaaggg atgacgcaca atcccactat
     1561 ccttcgcaag acccttcctc tatataagga agttcatttc atttggagag aacacggggg
     1621 actcaaacat tactcattca caaaaccatc ttaaagcaac tacacaagtc ttgaaatttt
     1681 ctcatatttt ctatttacta tataaacttt taatcaaatc aagattaaag ttaattaaat
     1741 gagcaaagga gaagaacttt tcactggagt tgtcccaatt cttgttgaat tagatggtga
     1801 tgttaatggg cacaaatttt ctgtccgtgg agagggtgaa ggtgatgcta caaacggaaa
     1861 actcaccctt aaatttattt gcactactgg aaaactacct gttccgtggc caacacttgt
     1921 cactactctg acctaaaagt tatcaggcat gcacctggta gctagtcttt aaaccaatag
     1981 attgcatcgg tttaaaaggc aagaccgtca aattgcggga aaggggtcaa cagccgttca
     2041 gtaccaagtc tcaggggaaa ctttgagatg gccttgcaaa gggtatggta ataagctgac
     2101 ggacatggtc ctaaccacgc agccaagtcc taagtcaaca gatcttctgt tgatatggat
     2161 gcagttcaca aactaaatgt cggtcgggga agatgtattc ttctcataag atatagtcgg
     2221 acctctcctt aatgggagct agcggatgaa gtgatgcaac actggagccg ctgggaacta
     2281 atttgtatgc gaaagtatat tgattagttt tggagtactc gatggtgttc aatgcttttc
     2341 ccgttatccg gatcacatga aacggcatga ctttttcaag agtgccatgc ccgaaggtta
     2401 tgtacaggaa cgcactatat ctttcaaaga tgacgggacc tacaagacgc gtgctgaagt
     2461 caagtttgaa ggtgataccc ttgttaatcg tatcgagtta aagggtattg attttaaaga
     2521 agatggaaac attcttggac acaaactcga gtacaacttt aactcacaca atgtatacat
     2581 cacggcagac aaacaaaaga atggaatcaa agctaacttc aaaattcgcc acaacgttga
     2641 agatggttcc gttcaactag cagaccatta tcaacaaaat actccaattg gcgatggccc
     2701 tgtcctttta ccagacaacc attacctgtc gacacaatct gtcctttcga aagatcccaa
     2761 cgaaaagcgt gaccacatgg tccttcttga gtttgtaact gctgctggga ttacacatgg
     2821 catggatgag ctctacaaag gaagcggagc tactaacttc agcctgctga agcaggctgg
     2881 agacgtggag gagaaccctg gacctatgac aaccttcaaa atcgagtccc ggatccacgg
     2941 caacctcaac ggggagaagt tcgagttggt tggaggtgga gtaggtgagg agggtcgcct
     3001 cgagattgag atgaagacta aagataaacc actggcattc tctcccttcc tgctgaccac
     3061 ttgcatgggt tacgggttct accacttcgc cagcttccca aaggggatta agaacatcta
     3121 tcttcatgct gcaacgaacg gaggttacac caacaccagg aaggagatct atgaagacgg
     3181 cggcatcttg gaggtcaact tccgttacac ttacgagttc aacaagatca tcggtgacgt
     3241 cgagtgcatt ggacatggat tcccaagtca gagtccgatc ttcaaggaca cgatcgtgaa
     3301 gagttgtccc acggtggacc tgatgttgcc aatgtccggg aacatcatcg ccagctccta
     3361 cgcttacgcc ttccaactga aggacggctc tttctacacg gcagaagtca agaacaacat
     3421 agacttcaag aatccaatcc acgagtcctt ctcgaagtca gggcccatgt tcacccacag
     3481 acgtgtcgag gagactctca ccaaggagaa ccttgccata gtggagtacc agcaggtttt
     3541 caacagcgcc ccaagagaca tgtagatatg aagatgaaga tgaaatattt ggtgtgtcaa
     3601 ataaaaagct tgtgtgctta agtttgtgtt tttttcttgg cttgttgtgt tatgaatttg
     3661 tggctttttc taatattaaa tgaatgtaag atctcattat aatgaataaa caaatgtttc
     3721 tataatccat tgtgaatgtt ttgttggatc tcttctgcag catataacta ctgtatgtgc
     3781 tatggtatgg actatggaat atgattaaag ataagatggg ctcatagagt aaaacgaggc
     3841 gagggaccta taaacctccc ttcatcatgc tatttcatga tctattttat aaaataaaga
     3901 tgtagaaaaa agtaagcgta ataaccgcaa aacaaatgat ttaaaacatg gcacataatg
     3961 aggagattaa gttcggttta cgtttatttt agtactaatt gtaacgtgag actacgtatc
     4021 gggaatcgcc taattaaagc attaatgcga acctgattag attcaccgac cctcctatcg
     4081 tgtcgacctt tctgtttctt agaatttttt ggtagtctat gtactaataa tgtcagcttc
     4141 gtatttattt cataagcaat ttgcatttgc aatttgtttt ttacttttat ttttattgta
     4201 ttgtggaatg tggactcgta ccaacatgaa gttatatacc accaaaaaaa ttacagttag
     4261 tcaaaagatt cacgagtgag agctacttat gattgtcttt tacgtatatg tctaattgtc
     4321 tatttgctca ataatctttg tactttcttt tgtcgttgat aaaatcacaa agttccaaaa
     4381 gtaatcgaat gatttgcttt taagaaaaga agagctcaat aattcaacat atatctgtac
     4441 acaattaccg cctttgagtg agctctagat gccacctgac gtctaagaag aattcgtaat
     4501 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     4561 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     4621 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     4681 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg ctagagcagc ttgccaacat
     4741 ggtggagcac gacactctcg tctactccaa gaatatcaaa gatacagtct cagaagacca
     4801 aagggctatt gagacttttc aacaaagggt aatatcggga aacctcctcg gattccattg
     4861 cccagctatc tgtcacttca tcaaaaggac agtagaaaag gaaggtggca cctacaaatg
     4921 ccatcattgc gataaaggaa aggctatcgt tcaagatgcc tctgccgaca gtggtcccaa
     4981 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc
     5041 aaagcaagtg gattgatgtg aacatggtgg agcacgacac tctcgtctac tccaagaata
     5101 tcaaagatac agtctcagaa gaccaaaggg ctattgagac ttttcaacaa agggtaatat
     5161 cgggaaacct cctcggattc cattgcccag ctatctgtca cttcatcaaa aggacagtag
     5221 aaaaggaagg tggcacctac aaatgccatc attgcgataa aggaaaggct atcgttcaag
     5281 atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc atcgtggaaa
     5341 aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgatatc tccactgacg
     5401 taagggatga cgcacaatcc cactatcctt cgcaagaccc ttcctctata taaggaagtt
     5461 catttcattt ggagaggaca cgctgaaatc accagtctct ctctacaaat ctatctctct
     5521 cgagctttcg cagatcccgg ggggcaatga gatatgggga ttgaacaaga tggattgcac
     5581 gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca
     5641 atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt
     5701 gtcaagaccg acctgtccgg tgccctgaat gaactccagg acgaggcagc gcggctatcg
     5761 tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga
     5821 agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct
     5881 cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg
     5941 gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg
     6001 gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc
     6061 gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacacat
     6121 ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac
     6181 tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt
     6241 gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct
     6301 cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg aagtagatgc
     6361 cgaccggatc tgtcgatcga caagctcgag tttctccata ataatgtgtg agtagttccc
     6421 agataaggga attagggttc ctatagggtt tcgctcatgt gttgagcata taagaaaccc
     6481 ttagtatgta tttgtatttg taaaatactt ctatcaataa aatttctaat tcctaaaacc
     6541 aaaatccagt actaaaatcc agatcccccg aattaattcg gcgttaattc agtacattaa
     6601 aaacgtccgc aatgtgttat taagttgtct aagcgtcaat ttgtttacac cacaatatat
     6661 cctgccacca gccagccaac agctccccga ccggcagctc ggcacaaaat caccactcga
     6721 tacaggcagc ccatcagtcc gggacggcgt cagcgggaga gccgttgtaa ggcggcagac
     6781 tttgctcatg ttaccgatgc tattcggaag aacggcaact aagctgccgg gtttgaaaca
     6841 cggatgatct cgcggagggt agcatgttga ttgtaacgat gacagagcgt tgctgcctgt
     6901 gatcaccgcg gtttcaaaat cggctccgtc gatactatgt tatacgccaa ctttgaaaac
     6961 aactttgaaa aagctgtttt ctggtattta aggttttaga atgcaaggaa cagtgaattg
     7021 gagttcgtct tgttataatt agcttcttgg ggtatcttta aatactgtag aaaagaggaa
     7081 ggaaataata aatggctaaa atgagaatat caccggaatt gaaaaaactg atcgaaaaat
     7141 accgctgcgt aaaagatacg gaaggaatgt ctcctgctaa ggtatataag ctggtgggag
     7201 aaaatgaaaa cctatattta aaaatgacgg acagccggta taaagggacc acctatgatg
     7261 tggaacggga aaaggacatg atgctatggc tggaaggaaa gctgcctgtt ccaaaggtcc
     7321 tgcactttga acggcatgat ggctggagca atctgctcat gagtgaggcc gatggcgtcc
     7381 tttgctcgga agagtatgaa gatgaacaaa gccctgaaaa gattatcgag ctgtatgcgg
     7441 agtgcatcag gctctttcac tccatcgaca tatcggattg tccctatacg aatagcttag
     7501 acagccgctt agccgaattg gattacttac tgaataacga tctggccgat gtggattgcg
     7561 aaaactggga agaagacact ccatttaaag atccgcgcga gctgtatgat tttttaaaga
     7621 cggaaaagcc cgaagaggaa cttgtctttt cccacggcga cctgggagac agcaacatct
     7681 ttgtgaaaga tggcaaagta agtggcttta ttgatcttgg gagaagcggc agggcggaca
     7741 agtggtatga cattgccttc tgcgtccggt cgatcaggga ggatatcggg gaagaacagt
     7801 atgtcgagct attttttgac ttactgggga tcaagcctga ttgggagaaa ataaaatatt
     7861 atattttact ggatgaattg ttttagtacc tagaatgcat gaccaaaatc ccttaacgtg
     7921 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     7981 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     8041 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     8101 cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact
     8161 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     8221 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     8281 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     8341 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     8401 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     8461 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     8521 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     8581 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     8641 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     8701 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt
     8761 ttctccttac gcatctgtgc ggtatttcac accgcatatg gtgcactctc agtacaatct
     8821 gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat
     8881 ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
     8941 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
     9001 accgtcatca ccgaaacgcg cgaggcaggg tgccttgatg tgggcgccgg cggtcgagtg
     9061 gcgacggcgc ggcttgtccg cgccctggta gattgcctgg ccgtaggcca gccatttttg
     9121 agcggccagc ggccgcgata ggccgacgcg aagcggcggg gcgtagggag cgcagcgacc
     9181 gaagggtagg cgctttttgc agctcttcgg ctgtgcgctg gccagacagt tatgcacagg
     9241 ccaggcgggt tttaagagtt ttaataagtt ttaaagagtt ttaggcggaa aaatcgcctt
     9301 ttttctcttt tatatcagtc acttacatgt gtgaccggtt cccaatgtac ggctttgggt
     9361 tcccaatgta cgggttccgg ttcccaatgt acggctttgg gttcccaatg tacgtgctat
     9421 ccacaggaaa gagacctttt cgaccttttt cccctgctag ggcaatttgc cctagcatct
     9481 gctccgtaca ttaggaaccg gcggatgctt cgccctcgat caggttgcgg tagcgcatga
     9541 ctaggatcgg gccagcctgc cccgcctcct ccttcaaatc gtactccggc aggtcatttg
     9601 acccgatcag cttgcgcacg gtgaaacaga acttcttgaa ctctccggcg ctgccactgc
     9661 gttcgtagat cgtcttgaac aaccatctgg cttctgcctt gcctgcggcg cggcgtgcca
     9721 ggcggtagag aaaacggccg atgccgggat cgatcaaaaa gtaatcgggg tgaaccgtca
     9781 gcacgtccgg gttcttgcct tctgtgatct cgcggtacat ccaatcagct agctcgatct
     9841 cgatgtactc cggccgcccg gtttcgctct ttacgatctt gtagcggcta atcaaggctt
     9901 caccctcgga taccgtcacc aggcggccgt tcttggcctt cttcgtacgc tgcatggcaa
     9961 cgtgcgtggt gtttaaccga atgcaggttt ctaccaggtc gtctttctgc tttccgccat
    10021 cggctcgccg gcagaacttg agtacgtccg caacgtgtgg acggaacacg cggccgggct
    10081 tgtctccctt cccttcccgg tatcggttca tggattcggt tagatgggaa accgccatca
    10141 gtaccaggtc gtaatcccac acactggcca tgccggccgg ccctgcggaa acctctacgt
    10201 gcccgtctgg aagctcgtag cggatcacct cgccagctcg tcggtcacgc ttcgacagac
    10261 ggaaaacggc cacgtccatg atgctgcgac tatcgcgggt gcccacgtca tagagcatcg
    10321 gaacgaaaaa atctggttgc tcgtcgccct tgggcggctt cctaatcgac ggcgcaccgg
    10381 ctgccggcgg ttgccgggat tctttgcgga ttcgatcagc ggccgcttgc cacgattcac
    10441 cggggcgtgc ttctgcctcg atgcgttgcc gctgggcggc ctgcgcggcc ttcaacttct
    10501 ccaccaggtc atcacccagc gccgcgccga tttgtaccgg gccggatggt ttgcgaccgc
    10561 tcacgccgat tcctcgggct tgggggttcc agtgccattg cagggccggc aggcaaccca
    10621 gccgcttacg cctggccaac cgcccgttcc tccacacatg gggcattcca cggcgtcggt
    10681 gcctggttgt tcttgatttt ccatgccgcc tcctttagcc gctaaaattc atctactcat
    10741 ttattcattt gctcatttac tctggtagct gcgcgatgta ttcagatagc agctcggtaa
    10801 tggtcttgcc ttggcgtacc gcgtacatct tcagcttggt gtgatcctcc gccggcaact
    10861 gaaagttgac ccgcttcatg gctggcgtgt ctgccaggct ggccaacgtt gcagccttgc
    10921 tgctgcgtgc gctcggacgg ccggcactta gcgtgtttgt gcttttgctc attttctctt
    10981 tacctcatta actcaaatga gttttgattt aatttcagcg gccagcgcct ggacctcgcg
    11041 ggcagcgtcg ccctcgggtt ctgattcaag aacggttgtg ccggcggcgg cagtgcctgg
    11101 gtagctcacg cgctgcgtga tacgggactc aagaatgggc agctcgtacc cggccagcgc
    11161 ctcggcaacc tcaccgccga tgcgcgtgcc tttgatcgcc cgcgacacga caaaggccgc
    11221 ttgtagcctt ccatccgtga cctcaatgcg ctgcttaacc agctccacca ggtcggcggt
    11281 ggcccatatg tcgtaagggc ttggctgcac cggaatcagc acgaagtcgg ctgccttgat
    11341 cgcggacaca gccaagtccg ccgcctgggg cgctccgtcg atcactacga agtcgcgccg
    11401 gccgatggcc ttcacgtcgc ggtcaatcgt cgggcggtcg atgccgacaa cggttagcgg
    11461 ttgatcttcc cgcacggccg cccaatcgcg ggcactgccc tggggatcgg aatcgactaa
    11521 cagaacatcg gccccggcga gttgcagggc gcgggctaga tgggttgcga tggtcgtctt
    11581 gcctgacccg cctttctggt taagtacagc gataaccttc atgcgttccc cttgcgtatt
    11641 tgtttattta ctcatcgcat catatacgca gcgaccgcat gacgcaagct gttttactca
    11701 aatacacatc acctttttag acggcggcgc tcggtttctt cagcggccaa gctggccggc
    11761 caggccgcca gcttggcatc agacaaaccg gccaggattt catgcagccg cacggttgag
    11821 acgtgcgcgg gcggctcgaa cacgtacccg gccgcgatca tctccgcctc gatctcttcg
    11881 gtaatgaaaa acggttcgtc ctggccgtcc tggtgcggtt tcatgcttgt tcctcttggc
    11941 gttcattctc ggcggccgcc agggcgtcgg cctcggtcaa tgcgtcctca cggaaggcac
    12001 cgcgccgcct ggcctcggtg ggcgtcactt cctcgctgcg ctcaagtgcg cggtacaggg
    12061 tcgagcgatg cacgccaagc agtgcagccg cctctttcac ggtgcggcct tcctggtcga
    12121 tcagctcgcg ggcgtgcgcg atctgtgccg gggtgagggt agggcggggg ccaaacttca
    12181 cgcctcgggc cttggcggcc tcgcgcccgc tccgggtgcg gtcgatgatt agggaacgct
    12241 cgaactcggc aatgccggcg aacacggtca acaccatgcg gccggccggc gtggtggtgt
    12301 cggcccacgg ctctgccagg ctacgcaggc ccgcgccggc ctcctggatg cgctcggcaa
    12361 tgtccagtag gtcgcgggtg ctgcgggcca ggcggtctag cctggtcact gtcacaacgt
    12421 cgccagggcg taggtggtca agcatcctgg ccagctccgg gcggtcgcgc ctggtgccgg
    12481 tgatcttctc ggaaaacagc ttggtgcagc cggccgcgtg cagttcggcc cgttggttgg
    12541 tcaagtcctg gtcgtcggtg ctgacgcggg catagcccag caggccagcg gcggcgctct
    12601 tgttcatggc gtaatgtctc cggttctagt cgcaagtatt ctactttatg cgactaaaac
    12661 acgcgacaag aaaacgccag gaaaagggca gggcggcagc ctgtcgcgta acttaggact
    12721 tgtgcgacat gtcgttttca gaagacggct gcactgaacg tcagaagccg actgcactat
    12781 agcagcggag gggttggatc aaagtacttt gatcccgagg ggaaccctgt ggttggcatg
    12841 cacatacaaa tggacgaacg gataaacctt ttcacgccct tttaaatatc cgattattct
    12901 aataaacgct cttttctctt aggtttaccc gccaatatat cctgtcaaac actgatagtt
    12961 taattcccga tctagtaaca tagatgacac cgcgcgcgat aatttatcct agtttgcgcg
    13021 ctatattttg ttttctatcg cgtattaaat gtataattgc gggactctaa tcataaaaac
    13081 ccatctcata aataacgtca tgcattacat gttaattatt acatgcttaa cgtaattcaa
    13141 cagaaattat atgataatca tcgcaagacc ggcaacagga ttcaatctta agaaacttta
    13201 ttgccaaatg tttgaacgat cggggaaatt cgagctggtc accaattcac acgtgatggt
    13261 gatggtgatg gctagcgttc ttgtagccga aatctggaat gttggtccag cgctcgcgaa
    13321 agacgtgcgc ggcgagcttc ggcttgcggt cacgagtgaa cacgcccttc ttgtttcctt
    13381 ggacgcgcat cacgccctga gaggtcgcga agtccgcgaa gttccacgct tgctcaccca
    13441 cgaagttctc aaactcatcg aacacgacgt ggttcgcctg gtagtactcg acttgatatt
    13501 cctcggtgaa catcactgga tcaatgtcgt gaaagcccgc aacggtgtct gcgccgtact
    13561 cagtgatcat gatcggcttt cctgggcaac gcttgttcca cgcgtgaaat tcctggcgga
    13621 gatggacttt ggccgcttcg agatcaccgc catcgaagta ccatccgtta tagcgattga
    13681 gcgcgatgac gtcaatcagt tcggcgactt tgtccgtctc cggggtagcc atcacaaaca
    13741 gcacgatcgt gaccggacgc ttctgtgggt cgagttcctt ggtcagctcc accaacggct
    13801 tgaagtactc gtacgcgccc tcttcctcag tcgccgcctc gttggcgatg ctccacatca
    13861 cgacgcttgg atggttcttg tcacgagaca ccagttcacg gagaacgtct tgatggtgct
    13921 caaacgtccg aatcttctcc caggtactga cgcgctcgct gccttcgccg agtcccgtgg
    13981 tggccatgaa gttgaggtgc acgccaactg ccggagtctc gtcgatcacg accagaccct
    14041 cgcgatccgc aagacgcatc aactcttcag agtacggata gtgtgcggtc cggaagctgt
    14101 tggcgccgat ccatttgagg atattgaaat ccatcacatt gctcgcttcg ttaaagccac
    14161 ggccgttgat aggagtgtcc tcatgtttgc caaagccctt gaagtagaac ggtttgttgt
    14221 tgatgaggaa cttgccgtcg ttgacttcca cggtccgcac gccgaacggc tcttcataga
    14281 catcgatggt cagtccgtcg ttcaccagtt ccactttgat ctggtagaga tacgtgttca
    14341 gtggttccca gaggatgaca ttcggaatct ccacgttacc gctcaggccc tcggtgcttg
    14401 cgaccacttt gccttcctca tccacgaccg acactttcac ggtctcggct ttgccttgaa
    14461 agtccaccgt ataggtcaca gtcccggttg ggccattgaa gtcggtcaca accgagatgt
    14521 cctcgacgta cgtaaacggg gtcgtgtaga ttttcaccgg acggtgcagg cctgcatagt
    14581 tgaagaagtc gaagttcggc ttgttacgaa tgacttttcc gaggccctct tcgtggcgct
    14641 cgctgtacag ccccaccggg agggtgctat cgtcgaggat gttgtccacg gcgacggtga
    14701 cgcgattcat gccatcacgc agcgagttgt tgatttccgc ttcgaatggc aggaatccgc
    14761 ccttgtgctc cacgaccagc tcaccattga catagacaat tgctttgtga gttgcagagc
    14821 cgaagcggag cacgatacgc tgatccttca gataggccgg caccgtgaac tcacgttcgt
    14881 accagacata tccgatatgg ttgcggattt ccttggtcac gccaatgtca ttgtaactgc
    14941 ttgggacggc catactaata gtgtcggtca gcttgctttc gtaccacttc tcttccagtc
    15001 ctttcccgta gtccagcttg aagttccaga cgccattgag gtcgaagacg ccacgggtct
    15061 cggtgttgat cgggtacaga ctagttcgtc ggttctgtaa ctatcatcat catcatagac
    15121 acacgaaata aagtaatcag attatcagtt aaagctatgt aatatttaca ccataaccaa
    15181 tcaattaaaa aatagatcag tttaaagaaa gatcaaagct caaaaaaata aaaagagaaa
    15241 agggtcctaa ccaagaaaat gaaggagaaa aactagaaat ttaccctcag atctaccatg
//
