LOCUS       sequence-477056-       14559 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of Ribozyme split GFPuv with gq2 motif.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14559)
  AUTHORS   Liu Y, Rajput R, Islam MT, Valle ID, Yao T, Agrawal R, Boone BA, 
            Eckert CA, Abraham PE, Chen JG, Tuskan GA, Yang X
  TITLE     A split ribozyme system for in vivo plant RNA imaging and genetic 
            engineering.
  JOURNAL   Plant Biotechnol J. 2025 May;23(5):1640-1649. doi: 
            10.1111/pbi.14612. Epub 2025 Feb 7.
  PUBMED    39919021
REFERENCE   2  (bases 1 to 14559)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/pbi.14612";
            journalName: "Plant Biotechnol J"; date: "2025-05"; volume: "23"; 
            issue: "5"; pages: "1640-1649"
COMMENT     Sequence Label: pXYB109
FEATURES             Location/Qualifiers
     source          1..14559
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(16..361)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     723..745
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     737..754
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     738..754
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1269..1614
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     terminator      2826..3074
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     primer_bind     complement(3764..3780)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3764..3780)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3777..3799)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3788..3804
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3812..3818,3819..3836,3837..3842))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3857..3878
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        4069..4745
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     CDS             4813..5610
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLL
                     SSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEH
                     QGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDI
                     ALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4870..4889)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5480..5499
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5650..5824
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    5902..5926
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6351..7145
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      7232..7820
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7721..7740
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7974..7991
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8006..8146
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8072..8091)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8232..8254
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8490..8684
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8750..9823)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10252..10881)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    12182..12206
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     terminator      complement(12228..12480)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(12512..12529)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(join(12536..14353,14544..14558))
                     /codon_start=1
                     /product="beta-glucuronidase"
                     /label=GUSPlus(TM)
                     /note="codon-optimized Staphylococcus gusA gene with a 
                     catalase intron to ensure expression in plants but not 
                     bacteria"
                     /translation="MVDLRNRRTSLYPINTETRGVFDLNGVWNFKLDYGKGLEEKWYES
                     KLTDTISMAVPSSYNDIGVTKEIRNHIGYVWYEREFTVPAYLKDQRIVLRFGSATHKAI
                     VYVNGELVVEHKGGFLPFEAEINNSLRDGMNRVTVAVDNILDDSTLPVGLYSERHEEGL
                     GKVIRNKPNFDFFNYAGLHRPVKIYTTPFTYVEDISVVTDFNGPTGTVTYTVDFQGKAE
                     TVKVSVVDEEGKVVASTEGLSGNVEIPNVILWEPLNTYLYQIKVELVNDGLTIDVYEEP
                     FGVRTVEVNDGKFLINNKPFYFKGFGKHEDTPINGRGFNEASNVMDFNILKWIGANSFR
                     TAHYPYSEELMRLADREGLVVIDETPAVGVHLNFMATTGLGEGSERVSTWEKIRTFEHH
                     QDVLRELVSRDKNHPSVVMWSIANEAATEEEGAYEYFKPLVELTKELDPQKRPVTIVLF
                     VMATPETDKVAELIDVIALNRYNGWYFDGGDLEAAKVHLRQEFHAWNKRCPGKPIMITE
                     YGADTVAGFHDIDPVMFTEEYQVEYYQANHVVFDEFENFVGEQAWNFADFATSQGVMRV
                     QGNKKGVFTRDRKPKLAAHVFRERWTNIPDFGYKN"
     intron          14354..14543
                     /label=cat1 intron
                     /note="castor bean catalase intron, modified"
ORIGIN
        1 gtcaagagtc ccccgtgttc tctccaaatg aaatgaactt ccttatatag aggaagggtc
       61 ttgcgaagga tagtgggatt gtgcgtcatc ccttacgtca gtggagatat cacatcaatc
      121 cacttgcttt gaagacgtgg ttggaacgtc ttctttttcc acgatgctcc tcgtgggtgg
      181 gggtccatct ttgggaccac tgtcggcaga ggcatcttca acgatggcct ttcctttatc
      241 gcaatgatgg catttgtagg agccaccttc cttttccact atcttcacaa taaagtgaca
      301 gatagctggg caatggaatc cgaggaggtt tccggatatt accctttgtt gaaaagtctc
      361 aattgccctt tggtcttctg agactgtatc tttgatattt ttggagtaga caagtgtgtc
      421 gtgctccacc atgttgacga agattttctt cttgtcattg agtcgtaaga gactctgtat
      481 gaactgttcg ccagtcttta cggcgagttc tgttaggtcc tctatttgaa tctttgactc
      541 catgaagcta aactgaaggc gggaaacgac aatctgatcc aagctcaagc tgctctagca
      601 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
      661 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt
      721 ttcccagtca cgacgttgta aaacgacggc cagtgccaag cttgcatgcc tgcaggtccc
      781 cagattagcc ttttcaattt cagaaagaat gctaacccac agatggttag agaggcttac
      841 gcagcaggtc tcatcaagac gatctacccg agcaataatc tccaggaaat caaatacctt
      901 cccaagaagg ttaaagatgc agtcaaaaga ttcaggacta actgcatcaa gaacacagag
      961 aaagatatat ttctcaagat cagaagtact attccagtat ggacgattca aggcttgctt
     1021 cacaaaccaa ggcaagtaat agagattgga gtctctaaaa aggtagttcc cactgaatca
     1081 aaggccatgg agtcaaagat tcaaatagag gacctaacag aactcgccgt aaagactggc
     1141 gaacagttca tacagagtct cttacgactc aatgacaaga agaaaatctt cgtcaacatg
     1201 gtggagcacg acacacttgt ctactccaaa aatatcaaag atacagtctc agaagaccaa
     1261 agggcaattg agacttttca acaaagggta atatccggaa acctcctcgg attccattgc
     1321 ccagctatct gtcactttat tgtgaagata gtggaaaagg aaggtggctc ctacaaatgc
     1381 catcattgcg ataaaggaaa ggccatcgtt gaagatgcct ctgccgacag tggtcccaaa
     1441 gatggacccc cacccacgag gagcatcgtg gaaaaagaag acgttccaac cacgtcttca
     1501 aagcaagtgg attgatgtga tatctccact gacgtaaggg atgacgcaca atcccactat
     1561 ccttcgcaag acccttcctc tatataagga agttcatttc atttggagag aacacggggg
     1621 actcaaacat tactcattca caaaaccatc ttaaagcaac tacacaagtc ttgaaatttt
     1681 ctcatatttt ctatttacta tataaacttt taatcaaatc aagattaaag ttaattaaat
     1741 gacaaccttc aaaatcgagt cccggatcca cggcaacctc aacggggaga agttcgagtt
     1801 ggttggaggt ggagtaggtg aggagggtcg cctcgagatt gagatgaaga ctaaagataa
     1861 accactggca ttctctccct tcctgctgac cacttgcatg ggttacgggt tctaccactt
     1921 cgccagcttc ccaaagggga ttaagaacat ctataaatag caatatttac ctttgggtca
     1981 aaaagttatc aggcatgcac ctggtagcta gtctttaaac caatagattg catcggttta
     2041 aaaggcaaga ccgtcaaatt gcgggaaagg ggtcaacagc cgttcagtac caagtctcag
     2101 gggaaacttt gagatggcct tgcaaagggt atggtaataa gctgacggac atggtcctaa
     2161 ccacgcagcc aagtcctaag tcaacagatc ttctgttgat atggatgcag ttcacagact
     2221 aaatgtcggt cggggaagat gtattcttct cataagatat agtcggacct ctccttaatg
     2281 ggagctagcg gatgaagtga tgcaacactg gagccgctgg gaactaattt gtatgcgaaa
     2341 gtatattgat tagttttgga gtactcgctt catgctgcaa cgaacggagg ttacaccaac
     2401 accaggaagg agatctatga agacggcggc atcttggagg tcaacttccg ttacacttac
     2461 gagttcaaca agatcatcgg tgacgtcgag tgcattggac atggattccc aagtcagagt
     2521 ccgatcttca aggacacgat cgtgaagagt tgtcccacgg tggacctgat gttgccaatg
     2581 tccgggaaca tcatcgccag ctcctacgct tacgccttcc aactgaagga cggctctttc
     2641 tacacggcag aagtcaagaa caacatagac ttcaagaatc caatccacga gtccttctcg
     2701 aagtcagggc ccatgttcac ccacagacgt gtcgaggaga ctctcaccaa ggagaacctt
     2761 gccatagtgg agtaccagca ggttttcaac agcgccccaa gagacatgta gtggtggtgg
     2821 tggtatatga agatgaagat gaaatatttg gtgtgtcaaa taaaaagctt gtgtgcttaa
     2881 gtttgtgttt ttttcttggc ttgttgtgtt atgaatttgt ggctttttct aatattaaat
     2941 gaatgtaaga tctcattata atgaataaac aaatgtttct ataatccatt gtgaatgttt
     3001 tgttggatct cttctgcagc atataactac tgtatgtgct atggtatgga ctatggaata
     3061 tgattaaaga taagatgggc tcatagagta aaacgaggcg agggacctat aaacctccct
     3121 tcatcatgct atttcatgat ctattttata aaataaagat gtagaaaaaa gtaagcgtaa
     3181 taaccgcaaa acaaatgatt taaaacatgg cacataatga ggagattaag ttcggtttac
     3241 gtttatttta gtactaattg taacgtgaga ctacgtatcg ggaatcgcct aattaaagca
     3301 ttaatgcgaa cctgattaga ttcaccgacc ctcctatcgt gtcgaccttt ctgtttctta
     3361 gaattttttg gtagtctatg tactaataat gtcagcttcg tatttatttc ataagcaatt
     3421 tgcatttgca atttgttttt tacttttatt tttattgtat tgtggaatgt ggactcgtac
     3481 caacatgaag ttatatacca ccaaaaaaat tacagttagt caaaagattc acgagtgaga
     3541 gctacttatg attgtctttt acgtatatgt ctaattgtct atttgctcaa taatctttgt
     3601 actttctttt gtcgttgata aaatcacaaa gttccaaaag taatcgaatg atttgctttt
     3661 aagaaaagaa gagctcaata attcaacata tatctgtaca caattaccgc ctttgagtga
     3721 gctctagatg ccacctgacg tctaagaaga attcgtaatc atggtcatag ctgtttcctg
     3781 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     3841 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     3901 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     3961 gaggcggttt gcgtattggc tagagcagct tgccaacatg gtggagcacg acactctcgt
     4021 ctactccaag aatatcaaag atacagtctc agaagaccaa agggctattg agacttttca
     4081 acaaagggta atatcgggaa acctcctcgg attccattgc ccagctatct gtcacttcat
     4141 caaaaggaca gtagaaaagg aaggtggcac ctacaaatgc catcattgcg ataaaggaaa
     4201 ggctatcgtt caagatgcct ctgccgacag tggtcccaaa gatggacccc cacccacgag
     4261 gagcatcgtg gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga
     4321 acatggtgga gcacgacact ctcgtctact ccaagaatat caaagataca gtctcagaag
     4381 accaaagggc tattgagact tttcaacaaa gggtaatatc gggaaacctc ctcggattcc
     4441 attgcccagc tatctgtcac ttcatcaaaa ggacagtaga aaaggaaggt ggcacctaca
     4501 aatgccatca ttgcgataaa ggaaaggcta tcgttcaaga tgcctctgcc gacagtggtc
     4561 ccaaagatgg acccccaccc acgaggagca tcgtggaaaa agaagacgtt ccaaccacgt
     4621 cttcaaagca agtggattga tgtgatatct ccactgacgt aagggatgac gcacaatccc
     4681 actatccttc gcaagaccct tcctctatat aaggaagttc atttcatttg gagaggacac
     4741 gctgaaatca ccagtctctc tctacaaatc tatctctctc gagctttcgc agatcccggg
     4801 gggcaatgag atatggggat tgaacaagat ggattgcacg caggttctcc ggccgcttgg
     4861 gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc
     4921 gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt
     4981 gccctgaatg aactccagga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt
     5041 ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc
     5101 gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc
     5161 atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac
     5221 caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag
     5281 gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag
     5341 gcgcgcatgc ccgacggcga ggatctcgtc gtgacacatg gcgatgcctg cttgccgaat
     5401 atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg
     5461 gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa
     5521 tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc
     5581 ttctatcgcc ttcttgacga gttcttctga agtagatgcc gaccggatct gtcgatcgac
     5641 aagctcgagt ttctccataa taatgtgtga gtagttccca gataagggaa ttagggttcc
     5701 tatagggttt cgctcatgtg ttgagcatat aagaaaccct tagtatgtat ttgtatttgt
     5761 aaaatacttc tatcaataaa atttctaatt cctaaaacca aaatccagta ctaaaatcca
     5821 gatcccccga attaattcgg cgttaattca gtacattaaa aacgtccgca atgtgttatt
     5881 aagttgtcta agcgtcaatt tgtttacacc acaatatatc ctgccaccag ccagccaaca
     5941 gctccccgac cggcagctcg gcacaaaatc accactcgat acaggcagcc catcagtccg
     6001 ggacggcgtc agcgggagag ccgttgtaag gcggcagact ttgctcatgt taccgatgct
     6061 attcggaaga acggcaacta agctgccggg tttgaaacac ggatgatctc gcggagggta
     6121 gcatgttgat tgtaacgatg acagagcgtt gctgcctgtg atcaccgcgg tttcaaaatc
     6181 ggctccgtcg atactatgtt atacgccaac tttgaaaaca actttgaaaa agctgttttc
     6241 tggtatttaa ggttttagaa tgcaaggaac agtgaattgg agttcgtctt gttataatta
     6301 gcttcttggg gtatctttaa atactgtaga aaagaggaag gaaataataa atggctaaaa
     6361 tgagaatatc accggaattg aaaaaactga tcgaaaaata ccgctgcgta aaagatacgg
     6421 aaggaatgtc tcctgctaag gtatataagc tggtgggaga aaatgaaaac ctatatttaa
     6481 aaatgacgga cagccggtat aaagggacca cctatgatgt ggaacgggaa aaggacatga
     6541 tgctatggct ggaaggaaag ctgcctgttc caaaggtcct gcactttgaa cggcatgatg
     6601 gctggagcaa tctgctcatg agtgaggccg atggcgtcct ttgctcggaa gagtatgaag
     6661 atgaacaaag ccctgaaaag attatcgagc tgtatgcgga gtgcatcagg ctctttcact
     6721 ccatcgacat atcggattgt ccctatacga atagcttaga cagccgctta gccgaattgg
     6781 attacttact gaataacgat ctggccgatg tggattgcga aaactgggaa gaagacactc
     6841 catttaaaga tccgcgcgag ctgtatgatt ttttaaagac ggaaaagccc gaagaggaac
     6901 ttgtcttttc ccacggcgac ctgggagaca gcaacatctt tgtgaaagat ggcaaagtaa
     6961 gtggctttat tgatcttggg agaagcggca gggcggacaa gtggtatgac attgccttct
     7021 gcgtccggtc gatcagggag gatatcgggg aagaacagta tgtcgagcta ttttttgact
     7081 tactggggat caagcctgat tgggagaaaa taaaatatta tattttactg gatgaattgt
     7141 tttagtacct agaatgcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     7201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     7261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     7321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     7381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     7441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     7501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     7561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     7621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     7681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     7741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     7801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     7861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     7921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     7981 cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg
     8041 gtatttcaca ccgcatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
     8101 gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc
     8161 aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc
     8221 tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc
     8281 gaggcagggt gccttgatgt gggcgccggc ggtcgagtgg cgacggcgcg gcttgtccgc
     8341 gccctggtag attgcctggc cgtaggccag ccatttttga gcggccagcg gccgcgatag
     8401 gccgacgcga agcggcgggg cgtagggagc gcagcgaccg aagggtaggc gctttttgca
     8461 gctcttcggc tgtgcgctgg ccagacagtt atgcacaggc caggcgggtt ttaagagttt
     8521 taataagttt taaagagttt taggcggaaa aatcgccttt tttctctttt atatcagtca
     8581 cttacatgtg tgaccggttc ccaatgtacg gctttgggtt cccaatgtac gggttccggt
     8641 tcccaatgta cggctttggg ttcccaatgt acgtgctatc cacaggaaag agaccttttc
     8701 gacctttttc ccctgctagg gcaatttgcc ctagcatctg ctccgtacat taggaaccgg
     8761 cggatgcttc gccctcgatc aggttgcggt agcgcatgac taggatcggg ccagcctgcc
     8821 ccgcctcctc cttcaaatcg tactccggca ggtcatttga cccgatcagc ttgcgcacgg
     8881 tgaaacagaa cttcttgaac tctccggcgc tgccactgcg ttcgtagatc gtcttgaaca
     8941 accatctggc ttctgccttg cctgcggcgc ggcgtgccag gcggtagaga aaacggccga
     9001 tgccgggatc gatcaaaaag taatcggggt gaaccgtcag cacgtccggg ttcttgcctt
     9061 ctgtgatctc gcggtacatc caatcagcta gctcgatctc gatgtactcc ggccgcccgg
     9121 tttcgctctt tacgatcttg tagcggctaa tcaaggcttc accctcggat accgtcacca
     9181 ggcggccgtt cttggccttc ttcgtacgct gcatggcaac gtgcgtggtg tttaaccgaa
     9241 tgcaggtttc taccaggtcg tctttctgct ttccgccatc ggctcgccgg cagaacttga
     9301 gtacgtccgc aacgtgtgga cggaacacgc ggccgggctt gtctcccttc ccttcccggt
     9361 atcggttcat ggattcggtt agatgggaaa ccgccatcag taccaggtcg taatcccaca
     9421 cactggccat gccggccggc cctgcggaaa cctctacgtg cccgtctgga agctcgtagc
     9481 ggatcacctc gccagctcgt cggtcacgct tcgacagacg gaaaacggcc acgtccatga
     9541 tgctgcgact atcgcgggtg cccacgtcat agagcatcgg aacgaaaaaa tctggttgct
     9601 cgtcgccctt gggcggcttc ctaatcgacg gcgcaccggc tgccggcggt tgccgggatt
     9661 ctttgcggat tcgatcagcg gccgcttgcc acgattcacc ggggcgtgct tctgcctcga
     9721 tgcgttgccg ctgggcggcc tgcgcggcct tcaacttctc caccaggtca tcacccagcg
     9781 ccgcgccgat ttgtaccggg ccggatggtt tgcgaccgct cacgccgatt cctcgggctt
     9841 gggggttcca gtgccattgc agggccggca ggcaacccag ccgcttacgc ctggccaacc
     9901 gcccgttcct ccacacatgg ggcattccac ggcgtcggtg cctggttgtt cttgattttc
     9961 catgccgcct cctttagccg ctaaaattca tctactcatt tattcatttg ctcatttact
    10021 ctggtagctg cgcgatgtat tcagatagca gctcggtaat ggtcttgcct tggcgtaccg
    10081 cgtacatctt cagcttggtg tgatcctccg ccggcaactg aaagttgacc cgcttcatgg
    10141 ctggcgtgtc tgccaggctg gccaacgttg cagccttgct gctgcgtgcg ctcggacggc
    10201 cggcacttag cgtgtttgtg cttttgctca ttttctcttt acctcattaa ctcaaatgag
    10261 ttttgattta atttcagcgg ccagcgcctg gacctcgcgg gcagcgtcgc cctcgggttc
    10321 tgattcaaga acggttgtgc cggcggcggc agtgcctggg tagctcacgc gctgcgtgat
    10381 acgggactca agaatgggca gctcgtaccc ggccagcgcc tcggcaacct caccgccgat
    10441 gcgcgtgcct ttgatcgccc gcgacacgac aaaggccgct tgtagccttc catccgtgac
    10501 ctcaatgcgc tgcttaacca gctccaccag gtcggcggtg gcccatatgt cgtaagggct
    10561 tggctgcacc ggaatcagca cgaagtcggc tgccttgatc gcggacacag ccaagtccgc
    10621 cgcctggggc gctccgtcga tcactacgaa gtcgcgccgg ccgatggcct tcacgtcgcg
    10681 gtcaatcgtc gggcggtcga tgccgacaac ggttagcggt tgatcttccc gcacggccgc
    10741 ccaatcgcgg gcactgccct ggggatcgga atcgactaac agaacatcgg ccccggcgag
    10801 ttgcagggcg cgggctagat gggttgcgat ggtcgtcttg cctgacccgc ctttctggtt
    10861 aagtacagcg ataaccttca tgcgttcccc ttgcgtattt gtttatttac tcatcgcatc
    10921 atatacgcag cgaccgcatg acgcaagctg ttttactcaa atacacatca cctttttaga
    10981 cggcggcgct cggtttcttc agcggccaag ctggccggcc aggccgccag cttggcatca
    11041 gacaaaccgg ccaggatttc atgcagccgc acggttgaga cgtgcgcggg cggctcgaac
    11101 acgtacccgg ccgcgatcat ctccgcctcg atctcttcgg taatgaaaaa cggttcgtcc
    11161 tggccgtcct ggtgcggttt catgcttgtt cctcttggcg ttcattctcg gcggccgcca
    11221 gggcgtcggc ctcggtcaat gcgtcctcac ggaaggcacc gcgccgcctg gcctcggtgg
    11281 gcgtcacttc ctcgctgcgc tcaagtgcgc ggtacagggt cgagcgatgc acgccaagca
    11341 gtgcagccgc ctctttcacg gtgcggcctt cctggtcgat cagctcgcgg gcgtgcgcga
    11401 tctgtgccgg ggtgagggta gggcgggggc caaacttcac gcctcgggcc ttggcggcct
    11461 cgcgcccgct ccgggtgcgg tcgatgatta gggaacgctc gaactcggca atgccggcga
    11521 acacggtcaa caccatgcgg ccggccggcg tggtggtgtc ggcccacggc tctgccaggc
    11581 tacgcaggcc cgcgccggcc tcctggatgc gctcggcaat gtccagtagg tcgcgggtgc
    11641 tgcgggccag gcggtctagc ctggtcactg tcacaacgtc gccagggcgt aggtggtcaa
    11701 gcatcctggc cagctccggg cggtcgcgcc tggtgccggt gatcttctcg gaaaacagct
    11761 tggtgcagcc ggccgcgtgc agttcggccc gttggttggt caagtcctgg tcgtcggtgc
    11821 tgacgcgggc atagcccagc aggccagcgg cggcgctctt gttcatggcg taatgtctcc
    11881 ggttctagtc gcaagtattc tactttatgc gactaaaaca cgcgacaaga aaacgccagg
    11941 aaaagggcag ggcggcagcc tgtcgcgtaa cttaggactt gtgcgacatg tcgttttcag
    12001 aagacggctg cactgaacgt cagaagccga ctgcactata gcagcggagg ggttggatca
    12061 aagtactttg atcccgaggg gaaccctgtg gttggcatgc acatacaaat ggacgaacgg
    12121 ataaaccttt tcacgccctt ttaaatatcc gattattcta ataaacgctc ttttctctta
    12181 ggtttacccg ccaatatatc ctgtcaaaca ctgatagttt aattcccgat ctagtaacat
    12241 agatgacacc gcgcgcgata atttatccta gtttgcgcgc tatattttgt tttctatcgc
    12301 gtattaaatg tataattgcg ggactctaat cataaaaacc catctcataa ataacgtcat
    12361 gcattacatg ttaattatta catgcttaac gtaattcaac agaaattata tgataatcat
    12421 cgcaagaccg gcaacaggat tcaatcttaa gaaactttat tgccaaatgt ttgaacgatc
    12481 ggggaaattc gagctggtca ccaattcaca cgtgatggtg atggtgatgg ctagcgttct
    12541 tgtagccgaa atctggaatg ttggtccagc gctcgcgaaa gacgtgcgcg gcgagcttcg
    12601 gcttgcggtc acgagtgaac acgcccttct tgtttccttg gacgcgcatc acgccctgag
    12661 aggtcgcgaa gtccgcgaag ttccacgctt gctcacccac gaagttctca aactcatcga
    12721 acacgacgtg gttcgcctgg tagtactcga cttgatattc ctcggtgaac atcactggat
    12781 caatgtcgtg aaagcccgca acggtgtctg cgccgtactc agtgatcatg atcggctttc
    12841 ctgggcaacg cttgttccac gcgtgaaatt cctggcggag atggactttg gccgcttcga
    12901 gatcaccgcc atcgaagtac catccgttat agcgattgag cgcgatgacg tcaatcagtt
    12961 cggcgacttt gtccgtctcc ggggtagcca tcacaaacag cacgatcgtg accggacgct
    13021 tctgtgggtc gagttccttg gtcagctcca ccaacggctt gaagtactcg tacgcgccct
    13081 cttcctcagt cgccgcctcg ttggcgatgc tccacatcac gacgcttgga tggttcttgt
    13141 cacgagacac cagttcacgg agaacgtctt gatggtgctc aaacgtccga atcttctccc
    13201 aggtactgac gcgctcgctg ccttcgccga gtcccgtggt ggccatgaag ttgaggtgca
    13261 cgccaactgc cggagtctcg tcgatcacga ccagaccctc gcgatccgca agacgcatca
    13321 actcttcaga gtacggatag tgtgcggtcc ggaagctgtt ggcgccgatc catttgagga
    13381 tattgaaatc catcacattg ctcgcttcgt taaagccacg gccgttgata ggagtgtcct
    13441 catgtttgcc aaagcccttg aagtagaacg gtttgttgtt gatgaggaac ttgccgtcgt
    13501 tgacttccac ggtccgcacg ccgaacggct cttcatagac atcgatggtc agtccgtcgt
    13561 tcaccagttc cactttgatc tggtagagat acgtgttcag tggttcccag aggatgacat
    13621 tcggaatctc cacgttaccg ctcaggccct cggtgcttgc gaccactttg ccttcctcat
    13681 ccacgaccga cactttcacg gtctcggctt tgccttgaaa gtccaccgta taggtcacag
    13741 tcccggttgg gccattgaag tcggtcacaa ccgagatgtc ctcgacgtac gtaaacgggg
    13801 tcgtgtagat tttcaccgga cggtgcaggc ctgcatagtt gaagaagtcg aagttcggct
    13861 tgttacgaat gacttttccg aggccctctt cgtggcgctc gctgtacagc cccaccggga
    13921 gggtgctatc gtcgaggatg ttgtccacgg cgacggtgac gcgattcatg ccatcacgca
    13981 gcgagttgtt gatttccgct tcgaatggca ggaatccgcc cttgtgctcc acgaccagct
    14041 caccattgac atagacaatt gctttgtgag ttgcagagcc gaagcggagc acgatacgct
    14101 gatccttcag ataggccggc accgtgaact cacgttcgta ccagacatat ccgatatggt
    14161 tgcggatttc cttggtcacg ccaatgtcat tgtaactgct tgggacggcc atactaatag
    14221 tgtcggtcag cttgctttcg taccacttct cttccagtcc tttcccgtag tccagcttga
    14281 agttccagac gccattgagg tcgaagacgc cacgggtctc ggtgttgatc gggtacagac
    14341 tagttcgtcg gttctgtaac tatcatcatc atcatagaca cacgaaataa agtaatcaga
    14401 ttatcagtta aagctatgta atatttacac cataaccaat caattaaaaa atagatcagt
    14461 ttaaagaaag atcaaagctc aaaaaaataa aaagagaaaa gggtcctaac caagaaaatg
    14521 aaggagaaaa actagaaatt taccctcaga tctaccatg
//
