LOCUS       sequence-477057-       14557 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression vector of deactivated ribozyme split GFPuv tEvopreq 
            motif.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14557)
  AUTHORS   Liu Y, Rajput R, Islam MT, Valle ID, Yao T, Agrawal R, Boone BA, 
            Eckert CA, Abraham PE, Chen JG, Tuskan GA, Yang X
  TITLE     A split ribozyme system for in vivo plant RNA imaging and genetic 
            engineering.
  JOURNAL   Plant Biotechnol J. 2025 May;23(5):1640-1649. doi: 
            10.1111/pbi.14612. Epub 2025 Feb 7.
  PUBMED    39919021
REFERENCE   2  (bases 1 to 14557)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/pbi.14612";
            journalName: "Plant Biotechnol J"; date: "2025-05"; volume: "23"; 
            issue: "5"; pages: "1640-1649"
COMMENT     Sequence Label: pXYB107
FEATURES             Location/Qualifiers
     source          1..14557
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(16..361)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     723..745
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     737..754
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     738..754
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1269..1614
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     terminator      2824..3072
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     primer_bind     complement(3762..3778)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3762..3778)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3775..3797)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3786..3802
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3810..3816,3817..3834,3835..3840))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3855..3876
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        4067..4743
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     CDS             4811..5608
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLL
                     SSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEH
                     QGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDI
                     ALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4868..4887)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5478..5497
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5648..5822
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    5900..5924
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6349..7143
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      7230..7818
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7719..7738
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7972..7989
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8004..8144
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8070..8089)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8230..8252
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8488..8682
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8748..9821)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10250..10879)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    12180..12204
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     terminator      complement(12226..12478)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(12510..12527)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(join(12534..14351,14542..14556))
                     /codon_start=1
                     /product="beta-glucuronidase"
                     /label=GUSPlus(TM)
                     /note="codon-optimized Staphylococcus gusA gene with a 
                     catalase intron to ensure expression in plants but not 
                     bacteria"
                     /translation="MVDLRNRRTSLYPINTETRGVFDLNGVWNFKLDYGKGLEEKWYES
                     KLTDTISMAVPSSYNDIGVTKEIRNHIGYVWYEREFTVPAYLKDQRIVLRFGSATHKAI
                     VYVNGELVVEHKGGFLPFEAEINNSLRDGMNRVTVAVDNILDDSTLPVGLYSERHEEGL
                     GKVIRNKPNFDFFNYAGLHRPVKIYTTPFTYVEDISVVTDFNGPTGTVTYTVDFQGKAE
                     TVKVSVVDEEGKVVASTEGLSGNVEIPNVILWEPLNTYLYQIKVELVNDGLTIDVYEEP
                     FGVRTVEVNDGKFLINNKPFYFKGFGKHEDTPINGRGFNEASNVMDFNILKWIGANSFR
                     TAHYPYSEELMRLADREGLVVIDETPAVGVHLNFMATTGLGEGSERVSTWEKIRTFEHH
                     QDVLRELVSRDKNHPSVVMWSIANEAATEEEGAYEYFKPLVELTKELDPQKRPVTIVLF
                     VMATPETDKVAELIDVIALNRYNGWYFDGGDLEAAKVHLRQEFHAWNKRCPGKPIMITE
                     YGADTVAGFHDIDPVMFTEEYQVEYYQANHVVFDEFENFVGEQAWNFADFATSQGVMRV
                     QGNKKGVFTRDRKPKLAAHVFRERWTNIPDFGYKN"
     intron          14352..14541
                     /label=cat1 intron
                     /note="castor bean catalase intron, modified"
ORIGIN
        1 gtcaagagtc ccccgtgttc tctccaaatg aaatgaactt ccttatatag aggaagggtc
       61 ttgcgaagga tagtgggatt gtgcgtcatc ccttacgtca gtggagatat cacatcaatc
      121 cacttgcttt gaagacgtgg ttggaacgtc ttctttttcc acgatgctcc tcgtgggtgg
      181 gggtccatct ttgggaccac tgtcggcaga ggcatcttca acgatggcct ttcctttatc
      241 gcaatgatgg catttgtagg agccaccttc cttttccact atcttcacaa taaagtgaca
      301 gatagctggg caatggaatc cgaggaggtt tccggatatt accctttgtt gaaaagtctc
      361 aattgccctt tggtcttctg agactgtatc tttgatattt ttggagtaga caagtgtgtc
      421 gtgctccacc atgttgacga agattttctt cttgtcattg agtcgtaaga gactctgtat
      481 gaactgttcg ccagtcttta cggcgagttc tgttaggtcc tctatttgaa tctttgactc
      541 catgaagcta aactgaaggc gggaaacgac aatctgatcc aagctcaagc tgctctagca
      601 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
      661 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt
      721 ttcccagtca cgacgttgta aaacgacggc cagtgccaag cttgcatgcc tgcaggtccc
      781 cagattagcc ttttcaattt cagaaagaat gctaacccac agatggttag agaggcttac
      841 gcagcaggtc tcatcaagac gatctacccg agcaataatc tccaggaaat caaatacctt
      901 cccaagaagg ttaaagatgc agtcaaaaga ttcaggacta actgcatcaa gaacacagag
      961 aaagatatat ttctcaagat cagaagtact attccagtat ggacgattca aggcttgctt
     1021 cacaaaccaa ggcaagtaat agagattgga gtctctaaaa aggtagttcc cactgaatca
     1081 aaggccatgg agtcaaagat tcaaatagag gacctaacag aactcgccgt aaagactggc
     1141 gaacagttca tacagagtct cttacgactc aatgacaaga agaaaatctt cgtcaacatg
     1201 gtggagcacg acacacttgt ctactccaaa aatatcaaag atacagtctc agaagaccaa
     1261 agggcaattg agacttttca acaaagggta atatccggaa acctcctcgg attccattgc
     1321 ccagctatct gtcactttat tgtgaagata gtggaaaagg aaggtggctc ctacaaatgc
     1381 catcattgcg ataaaggaaa ggccatcgtt gaagatgcct ctgccgacag tggtcccaaa
     1441 gatggacccc cacccacgag gagcatcgtg gaaaaagaag acgttccaac cacgtcttca
     1501 aagcaagtgg attgatgtga tatctccact gacgtaaggg atgacgcaca atcccactat
     1561 ccttcgcaag acccttcctc tatataagga agttcatttc atttggagag aacacggggg
     1621 actcaaacat tactcattca caaaaccatc ttaaagcaac tacacaagtc ttgaaatttt
     1681 ctcatatttt ctatttacta tataaacttt taatcaaatc aagattaaag ttaattaaat
     1741 gacaaccttc aaaatcgagt cccggatcca cggcaacctc aacggggaga agttcgagtt
     1801 ggttggaggt ggagtaggtg aggagggtcg cctcgagatt gagatgaaga ctaaagataa
     1861 accactggca ttctctccct tcctgctgac cacttgcatg ggttacgggt tctaccactt
     1921 cgccagcttc ccaaagggga ttaagaacat ctataaaagt tatcaggcat gcacctggta
     1981 gctagtcttt aaaccaatag attgcatcgg tttaaaaggc aagaccgtca aattgcggga
     2041 aaggggtcaa cagccgttca gtaccaagtc tcaggggaaa ctttgagatg gccttgcaaa
     2101 gggtatggta ataagctgac ggacatggtc ctaaccacgc agccaagtcc taagtcaaca
     2161 gatcttctgt tgatatggat gcagttcaca aactaaatgt cggtcgggga agatgtattc
     2221 ttctcataag atatagtcgg acctctcctt aatgggagct agcggatgaa gtgatgcaac
     2281 actggagccg ctgggaacta atttgtatgc gaaagtatat tgattagttt tggagtactc
     2341 gcttcatgct gcaacgaacg gaggttacac caacaccagg aaggagatct atgaagacgg
     2401 cggcatcttg gaggtcaact tccgttacac ttacgagttc aacaagatca tcggtgacgt
     2461 cgagtgcatt ggacatggat tcccaagtca gagtccgatc ttcaaggaca cgatcgtgaa
     2521 gagttgtccc acggtggacc tgatgttgcc aatgtccggg aacatcatcg ccagctccta
     2581 cgcttacgcc ttccaactga aggacggctc tttctacacg gcagaagtca agaacaacat
     2641 agacttcaag aatccaatcc acgagtcctt ctcgaagtca gggcccatgt tcacccacag
     2701 acgtgtcgag gagactctca ccaaggagaa ccttgccata gtggagtacc agcaggtttt
     2761 caacagcgcc ccaagagaca tgtagcgcgg ttctatctag ttacgcgtta aaccaactag
     2821 aaatatgaag atgaagatga aatatttggt gtgtcaaata aaaagcttgt gtgcttaagt
     2881 ttgtgttttt ttcttggctt gttgtgttat gaatttgtgg ctttttctaa tattaaatga
     2941 atgtaagatc tcattataat gaataaacaa atgtttctat aatccattgt gaatgttttg
     3001 ttggatctct tctgcagcat ataactactg tatgtgctat ggtatggact atggaatatg
     3061 attaaagata agatgggctc atagagtaaa acgaggcgag ggacctataa acctcccttc
     3121 atcatgctat ttcatgatct attttataaa ataaagatgt agaaaaaagt aagcgtaata
     3181 accgcaaaac aaatgattta aaacatggca cataatgagg agattaagtt cggtttacgt
     3241 ttattttagt actaattgta acgtgagact acgtatcggg aatcgcctaa ttaaagcatt
     3301 aatgcgaacc tgattagatt caccgaccct cctatcgtgt cgacctttct gtttcttaga
     3361 attttttggt agtctatgta ctaataatgt cagcttcgta tttatttcat aagcaatttg
     3421 catttgcaat ttgtttttta cttttatttt tattgtattg tggaatgtgg actcgtacca
     3481 acatgaagtt atataccacc aaaaaaatta cagttagtca aaagattcac gagtgagagc
     3541 tacttatgat tgtcttttac gtatatgtct aattgtctat ttgctcaata atctttgtac
     3601 tttcttttgt cgttgataaa atcacaaagt tccaaaagta atcgaatgat ttgcttttaa
     3661 gaaaagaaga gctcaataat tcaacatata tctgtacaca attaccgcct ttgagtgagc
     3721 tctagatgcc acctgacgtc taagaagaat tcgtaatcat ggtcatagct gtttcctgtg
     3781 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     3841 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     3901 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     3961 ggcggtttgc gtattggcta gagcagcttg ccaacatggt ggagcacgac actctcgtct
     4021 actccaagaa tatcaaagat acagtctcag aagaccaaag ggctattgag acttttcaac
     4081 aaagggtaat atcgggaaac ctcctcggat tccattgccc agctatctgt cacttcatca
     4141 aaaggacagt agaaaaggaa ggtggcacct acaaatgcca tcattgcgat aaaggaaagg
     4201 ctatcgttca agatgcctct gccgacagtg gtcccaaaga tggaccccca cccacgagga
     4261 gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa gcaagtggat tgatgtgaac
     4321 atggtggagc acgacactct cgtctactcc aagaatatca aagatacagt ctcagaagac
     4381 caaagggcta ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct cggattccat
     4441 tgcccagcta tctgtcactt catcaaaagg acagtagaaa aggaaggtgg cacctacaaa
     4501 tgccatcatt gcgataaagg aaaggctatc gttcaagatg cctctgccga cagtggtccc
     4561 aaagatggac ccccacccac gaggagcatc gtggaaaaag aagacgttcc aaccacgtct
     4621 tcaaagcaag tggattgatg tgatatctcc actgacgtaa gggatgacgc acaatcccac
     4681 tatccttcgc aagacccttc ctctatataa ggaagttcat ttcatttgga gaggacacgc
     4741 tgaaatcacc agtctctctc tacaaatcta tctctctcga gctttcgcag atcccggggg
     4801 gcaatgagat atggggattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt
     4861 ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt
     4921 gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc
     4981 cctgaatgaa ctccaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc
     5041 ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga
     5101 agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat
     5161 ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca
     5221 agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga
     5281 tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc
     5341 gcgcatgccc gacggcgagg atctcgtcgt gacacatggc gatgcctgct tgccgaatat
     5401 catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga
     5461 ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg
     5521 ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt
     5581 ctatcgcctt cttgacgagt tcttctgaag tagatgccga ccggatctgt cgatcgacaa
     5641 gctcgagttt ctccataata atgtgtgagt agttcccaga taagggaatt agggttccta
     5701 tagggtttcg ctcatgtgtt gagcatataa gaaaccctta gtatgtattt gtatttgtaa
     5761 aatacttcta tcaataaaat ttctaattcc taaaaccaaa atccagtact aaaatccaga
     5821 tcccccgaat taattcggcg ttaattcagt acattaaaaa cgtccgcaat gtgttattaa
     5881 gttgtctaag cgtcaatttg tttacaccac aatatatcct gccaccagcc agccaacagc
     5941 tccccgaccg gcagctcggc acaaaatcac cactcgatac aggcagccca tcagtccggg
     6001 acggcgtcag cgggagagcc gttgtaaggc ggcagacttt gctcatgtta ccgatgctat
     6061 tcggaagaac ggcaactaag ctgccgggtt tgaaacacgg atgatctcgc ggagggtagc
     6121 atgttgattg taacgatgac agagcgttgc tgcctgtgat caccgcggtt tcaaaatcgg
     6181 ctccgtcgat actatgttat acgccaactt tgaaaacaac tttgaaaaag ctgttttctg
     6241 gtatttaagg ttttagaatg caaggaacag tgaattggag ttcgtcttgt tataattagc
     6301 ttcttggggt atctttaaat actgtagaaa agaggaagga aataataaat ggctaaaatg
     6361 agaatatcac cggaattgaa aaaactgatc gaaaaatacc gctgcgtaaa agatacggaa
     6421 ggaatgtctc ctgctaaggt atataagctg gtgggagaaa atgaaaacct atatttaaaa
     6481 atgacggaca gccggtataa agggaccacc tatgatgtgg aacgggaaaa ggacatgatg
     6541 ctatggctgg aaggaaagct gcctgttcca aaggtcctgc actttgaacg gcatgatggc
     6601 tggagcaatc tgctcatgag tgaggccgat ggcgtccttt gctcggaaga gtatgaagat
     6661 gaacaaagcc ctgaaaagat tatcgagctg tatgcggagt gcatcaggct ctttcactcc
     6721 atcgacatat cggattgtcc ctatacgaat agcttagaca gccgcttagc cgaattggat
     6781 tacttactga ataacgatct ggccgatgtg gattgcgaaa actgggaaga agacactcca
     6841 tttaaagatc cgcgcgagct gtatgatttt ttaaagacgg aaaagcccga agaggaactt
     6901 gtcttttccc acggcgacct gggagacagc aacatctttg tgaaagatgg caaagtaagt
     6961 ggctttattg atcttgggag aagcggcagg gcggacaagt ggtatgacat tgccttctgc
     7021 gtccggtcga tcagggagga tatcggggaa gaacagtatg tcgagctatt ttttgactta
     7081 ctggggatca agcctgattg ggagaaaata aaatattata ttttactgga tgaattgttt
     7141 tagtacctag aatgcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     7201 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     7261 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     7321 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt
     7381 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     7441 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     7501 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     7561 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     7621 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     7681 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     7741 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     7801 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     7861 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     7921 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     7981 gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt
     8041 atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
     8101 cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa
     8161 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
     8221 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
     8281 ggcagggtgc cttgatgtgg gcgccggcgg tcgagtggcg acggcgcggc ttgtccgcgc
     8341 cctggtagat tgcctggccg taggccagcc atttttgagc ggccagcggc cgcgataggc
     8401 cgacgcgaag cggcggggcg tagggagcgc agcgaccgaa gggtaggcgc tttttgcagc
     8461 tcttcggctg tgcgctggcc agacagttat gcacaggcca ggcgggtttt aagagtttta
     8521 ataagtttta aagagtttta ggcggaaaaa tcgccttttt tctcttttat atcagtcact
     8581 tacatgtgtg accggttccc aatgtacggc tttgggttcc caatgtacgg gttccggttc
     8641 ccaatgtacg gctttgggtt cccaatgtac gtgctatcca caggaaagag accttttcga
     8701 cctttttccc ctgctagggc aatttgccct agcatctgct ccgtacatta ggaaccggcg
     8761 gatgcttcgc cctcgatcag gttgcggtag cgcatgacta ggatcgggcc agcctgcccc
     8821 gcctcctcct tcaaatcgta ctccggcagg tcatttgacc cgatcagctt gcgcacggtg
     8881 aaacagaact tcttgaactc tccggcgctg ccactgcgtt cgtagatcgt cttgaacaac
     8941 catctggctt ctgccttgcc tgcggcgcgg cgtgccaggc ggtagagaaa acggccgatg
     9001 ccgggatcga tcaaaaagta atcggggtga accgtcagca cgtccgggtt cttgccttct
     9061 gtgatctcgc ggtacatcca atcagctagc tcgatctcga tgtactccgg ccgcccggtt
     9121 tcgctcttta cgatcttgta gcggctaatc aaggcttcac cctcggatac cgtcaccagg
     9181 cggccgttct tggccttctt cgtacgctgc atggcaacgt gcgtggtgtt taaccgaatg
     9241 caggtttcta ccaggtcgtc tttctgcttt ccgccatcgg ctcgccggca gaacttgagt
     9301 acgtccgcaa cgtgtggacg gaacacgcgg ccgggcttgt ctcccttccc ttcccggtat
     9361 cggttcatgg attcggttag atgggaaacc gccatcagta ccaggtcgta atcccacaca
     9421 ctggccatgc cggccggccc tgcggaaacc tctacgtgcc cgtctggaag ctcgtagcgg
     9481 atcacctcgc cagctcgtcg gtcacgcttc gacagacgga aaacggccac gtccatgatg
     9541 ctgcgactat cgcgggtgcc cacgtcatag agcatcggaa cgaaaaaatc tggttgctcg
     9601 tcgcccttgg gcggcttcct aatcgacggc gcaccggctg ccggcggttg ccgggattct
     9661 ttgcggattc gatcagcggc cgcttgccac gattcaccgg ggcgtgcttc tgcctcgatg
     9721 cgttgccgct gggcggcctg cgcggccttc aacttctcca ccaggtcatc acccagcgcc
     9781 gcgccgattt gtaccgggcc ggatggtttg cgaccgctca cgccgattcc tcgggcttgg
     9841 gggttccagt gccattgcag ggccggcagg caacccagcc gcttacgcct ggccaaccgc
     9901 ccgttcctcc acacatgggg cattccacgg cgtcggtgcc tggttgttct tgattttcca
     9961 tgccgcctcc tttagccgct aaaattcatc tactcattta ttcatttgct catttactct
    10021 ggtagctgcg cgatgtattc agatagcagc tcggtaatgg tcttgccttg gcgtaccgcg
    10081 tacatcttca gcttggtgtg atcctccgcc ggcaactgaa agttgacccg cttcatggct
    10141 ggcgtgtctg ccaggctggc caacgttgca gccttgctgc tgcgtgcgct cggacggccg
    10201 gcacttagcg tgtttgtgct tttgctcatt ttctctttac ctcattaact caaatgagtt
    10261 ttgatttaat ttcagcggcc agcgcctgga cctcgcgggc agcgtcgccc tcgggttctg
    10321 attcaagaac ggttgtgccg gcggcggcag tgcctgggta gctcacgcgc tgcgtgatac
    10381 gggactcaag aatgggcagc tcgtacccgg ccagcgcctc ggcaacctca ccgccgatgc
    10441 gcgtgccttt gatcgcccgc gacacgacaa aggccgcttg tagccttcca tccgtgacct
    10501 caatgcgctg cttaaccagc tccaccaggt cggcggtggc ccatatgtcg taagggcttg
    10561 gctgcaccgg aatcagcacg aagtcggctg ccttgatcgc ggacacagcc aagtccgccg
    10621 cctggggcgc tccgtcgatc actacgaagt cgcgccggcc gatggccttc acgtcgcggt
    10681 caatcgtcgg gcggtcgatg ccgacaacgg ttagcggttg atcttcccgc acggccgccc
    10741 aatcgcgggc actgccctgg ggatcggaat cgactaacag aacatcggcc ccggcgagtt
    10801 gcagggcgcg ggctagatgg gttgcgatgg tcgtcttgcc tgacccgcct ttctggttaa
    10861 gtacagcgat aaccttcatg cgttcccctt gcgtatttgt ttatttactc atcgcatcat
    10921 atacgcagcg accgcatgac gcaagctgtt ttactcaaat acacatcacc tttttagacg
    10981 gcggcgctcg gtttcttcag cggccaagct ggccggccag gccgccagct tggcatcaga
    11041 caaaccggcc aggatttcat gcagccgcac ggttgagacg tgcgcgggcg gctcgaacac
    11101 gtacccggcc gcgatcatct ccgcctcgat ctcttcggta atgaaaaacg gttcgtcctg
    11161 gccgtcctgg tgcggtttca tgcttgttcc tcttggcgtt cattctcggc ggccgccagg
    11221 gcgtcggcct cggtcaatgc gtcctcacgg aaggcaccgc gccgcctggc ctcggtgggc
    11281 gtcacttcct cgctgcgctc aagtgcgcgg tacagggtcg agcgatgcac gccaagcagt
    11341 gcagccgcct ctttcacggt gcggccttcc tggtcgatca gctcgcgggc gtgcgcgatc
    11401 tgtgccgggg tgagggtagg gcgggggcca aacttcacgc ctcgggcctt ggcggcctcg
    11461 cgcccgctcc gggtgcggtc gatgattagg gaacgctcga actcggcaat gccggcgaac
    11521 acggtcaaca ccatgcggcc ggccggcgtg gtggtgtcgg cccacggctc tgccaggcta
    11581 cgcaggcccg cgccggcctc ctggatgcgc tcggcaatgt ccagtaggtc gcgggtgctg
    11641 cgggccaggc ggtctagcct ggtcactgtc acaacgtcgc cagggcgtag gtggtcaagc
    11701 atcctggcca gctccgggcg gtcgcgcctg gtgccggtga tcttctcgga aaacagcttg
    11761 gtgcagccgg ccgcgtgcag ttcggcccgt tggttggtca agtcctggtc gtcggtgctg
    11821 acgcgggcat agcccagcag gccagcggcg gcgctcttgt tcatggcgta atgtctccgg
    11881 ttctagtcgc aagtattcta ctttatgcga ctaaaacacg cgacaagaaa acgccaggaa
    11941 aagggcaggg cggcagcctg tcgcgtaact taggacttgt gcgacatgtc gttttcagaa
    12001 gacggctgca ctgaacgtca gaagccgact gcactatagc agcggagggg ttggatcaaa
    12061 gtactttgat cccgagggga accctgtggt tggcatgcac atacaaatgg acgaacggat
    12121 aaaccttttc acgccctttt aaatatccga ttattctaat aaacgctctt ttctcttagg
    12181 tttacccgcc aatatatcct gtcaaacact gatagtttaa ttcccgatct agtaacatag
    12241 atgacaccgc gcgcgataat ttatcctagt ttgcgcgcta tattttgttt tctatcgcgt
    12301 attaaatgta taattgcggg actctaatca taaaaaccca tctcataaat aacgtcatgc
    12361 attacatgtt aattattaca tgcttaacgt aattcaacag aaattatatg ataatcatcg
    12421 caagaccggc aacaggattc aatcttaaga aactttattg ccaaatgttt gaacgatcgg
    12481 ggaaattcga gctggtcacc aattcacacg tgatggtgat ggtgatggct agcgttcttg
    12541 tagccgaaat ctggaatgtt ggtccagcgc tcgcgaaaga cgtgcgcggc gagcttcggc
    12601 ttgcggtcac gagtgaacac gcccttcttg tttccttgga cgcgcatcac gccctgagag
    12661 gtcgcgaagt ccgcgaagtt ccacgcttgc tcacccacga agttctcaaa ctcatcgaac
    12721 acgacgtggt tcgcctggta gtactcgact tgatattcct cggtgaacat cactggatca
    12781 atgtcgtgaa agcccgcaac ggtgtctgcg ccgtactcag tgatcatgat cggctttcct
    12841 gggcaacgct tgttccacgc gtgaaattcc tggcggagat ggactttggc cgcttcgaga
    12901 tcaccgccat cgaagtacca tccgttatag cgattgagcg cgatgacgtc aatcagttcg
    12961 gcgactttgt ccgtctccgg ggtagccatc acaaacagca cgatcgtgac cggacgcttc
    13021 tgtgggtcga gttccttggt cagctccacc aacggcttga agtactcgta cgcgccctct
    13081 tcctcagtcg ccgcctcgtt ggcgatgctc cacatcacga cgcttggatg gttcttgtca
    13141 cgagacacca gttcacggag aacgtcttga tggtgctcaa acgtccgaat cttctcccag
    13201 gtactgacgc gctcgctgcc ttcgccgagt cccgtggtgg ccatgaagtt gaggtgcacg
    13261 ccaactgccg gagtctcgtc gatcacgacc agaccctcgc gatccgcaag acgcatcaac
    13321 tcttcagagt acggatagtg tgcggtccgg aagctgttgg cgccgatcca tttgaggata
    13381 ttgaaatcca tcacattgct cgcttcgtta aagccacggc cgttgatagg agtgtcctca
    13441 tgtttgccaa agcccttgaa gtagaacggt ttgttgttga tgaggaactt gccgtcgttg
    13501 acttccacgg tccgcacgcc gaacggctct tcatagacat cgatggtcag tccgtcgttc
    13561 accagttcca ctttgatctg gtagagatac gtgttcagtg gttcccagag gatgacattc
    13621 ggaatctcca cgttaccgct caggccctcg gtgcttgcga ccactttgcc ttcctcatcc
    13681 acgaccgaca ctttcacggt ctcggctttg ccttgaaagt ccaccgtata ggtcacagtc
    13741 ccggttgggc cattgaagtc ggtcacaacc gagatgtcct cgacgtacgt aaacggggtc
    13801 gtgtagattt tcaccggacg gtgcaggcct gcatagttga agaagtcgaa gttcggcttg
    13861 ttacgaatga cttttccgag gccctcttcg tggcgctcgc tgtacagccc caccgggagg
    13921 gtgctatcgt cgaggatgtt gtccacggcg acggtgacgc gattcatgcc atcacgcagc
    13981 gagttgttga tttccgcttc gaatggcagg aatccgccct tgtgctccac gaccagctca
    14041 ccattgacat agacaattgc tttgtgagtt gcagagccga agcggagcac gatacgctga
    14101 tccttcagat aggccggcac cgtgaactca cgttcgtacc agacatatcc gatatggttg
    14161 cggatttcct tggtcacgcc aatgtcattg taactgcttg ggacggccat actaatagtg
    14221 tcggtcagct tgctttcgta ccacttctct tccagtcctt tcccgtagtc cagcttgaag
    14281 ttccagacgc cattgaggtc gaagacgcca cgggtctcgg tgttgatcgg gtacagacta
    14341 gttcgtcggt tctgtaacta tcatcatcat catagacaca cgaaataaag taatcagatt
    14401 atcagttaaa gctatgtaat atttacacca taaccaatca attaaaaaat agatcagttt
    14461 aaagaaagat caaagctcaa aaaaataaaa agagaaaagg gtcctaacca agaaaatgaa
    14521 ggagaaaaac tagaaattta ccctcagatc taccatg
//
