LOCUS       sequence-477060-       14146 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression vector of GFPuv with 3' gq2 motif.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14146)
  AUTHORS   Liu Y, Rajput R, Islam MT, Valle ID, Yao T, Agrawal R, Boone BA, 
            Eckert CA, Abraham PE, Chen JG, Tuskan GA, Yang X
  TITLE     A split ribozyme system for in vivo plant RNA imaging and genetic 
            engineering.
  JOURNAL   Plant Biotechnol J. 2025 May;23(5):1640-1649. doi: 
            10.1111/pbi.14612. Epub 2025 Feb 7.
  PUBMED    39919021
REFERENCE   2  (bases 1 to 14146)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/pbi.14612";
            journalName: "Plant Biotechnol J"; date: "2025-05"; volume: "23"; 
            issue: "5"; pages: "1640-1649"
COMMENT     Sequence Label: pXYB108
FEATURES             Location/Qualifiers
     source          1..14146
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(16..361)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     723..745
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     737..754
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     738..754
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1269..1614
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     terminator      2413..2661
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     primer_bind     complement(3351..3367)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3351..3367)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3364..3386)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3375..3391
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3399..3405,3406..3423,3424..3429))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3444..3465
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        3656..4332
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     CDS             4400..5197
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLL
                     SSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEH
                     QGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDI
                     ALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4457..4476)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5067..5086
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5237..5411
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    5489..5513
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             5938..6732
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      6819..7407
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7308..7327
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7561..7578
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7593..7733
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7659..7678)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7819..7841
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8077..8271
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8337..9410)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(9839..10468)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    11769..11793
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     terminator      complement(11815..12067)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(12099..12116)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(join(12123..13940,14131..14145))
                     /codon_start=1
                     /product="beta-glucuronidase"
                     /label=GUSPlus(TM)
                     /note="codon-optimized Staphylococcus gusA gene with a 
                     catalase intron to ensure expression in plants but not 
                     bacteria"
                     /translation="MVDLRNRRTSLYPINTETRGVFDLNGVWNFKLDYGKGLEEKWYES
                     KLTDTISMAVPSSYNDIGVTKEIRNHIGYVWYEREFTVPAYLKDQRIVLRFGSATHKAI
                     VYVNGELVVEHKGGFLPFEAEINNSLRDGMNRVTVAVDNILDDSTLPVGLYSERHEEGL
                     GKVIRNKPNFDFFNYAGLHRPVKIYTTPFTYVEDISVVTDFNGPTGTVTYTVDFQGKAE
                     TVKVSVVDEEGKVVASTEGLSGNVEIPNVILWEPLNTYLYQIKVELVNDGLTIDVYEEP
                     FGVRTVEVNDGKFLINNKPFYFKGFGKHEDTPINGRGFNEASNVMDFNILKWIGANSFR
                     TAHYPYSEELMRLADREGLVVIDETPAVGVHLNFMATTGLGEGSERVSTWEKIRTFEHH
                     QDVLRELVSRDKNHPSVVMWSIANEAATEEEGAYEYFKPLVELTKELDPQKRPVTIVLF
                     VMATPETDKVAELIDVIALNRYNGWYFDGGDLEAAKVHLRQEFHAWNKRCPGKPIMITE
                     YGADTVAGFHDIDPVMFTEEYQVEYYQANHVVFDEFENFVGEQAWNFADFATSQGVMRV
                     QGNKKGVFTRDRKPKLAAHVFRERWTNIPDFGYKN"
     intron          13941..14130
                     /label=cat1 intron
                     /note="castor bean catalase intron, modified"
ORIGIN
        1 gtcaagagtc ccccgtgttc tctccaaatg aaatgaactt ccttatatag aggaagggtc
       61 ttgcgaagga tagtgggatt gtgcgtcatc ccttacgtca gtggagatat cacatcaatc
      121 cacttgcttt gaagacgtgg ttggaacgtc ttctttttcc acgatgctcc tcgtgggtgg
      181 gggtccatct ttgggaccac tgtcggcaga ggcatcttca acgatggcct ttcctttatc
      241 gcaatgatgg catttgtagg agccaccttc cttttccact atcttcacaa taaagtgaca
      301 gatagctggg caatggaatc cgaggaggtt tccggatatt accctttgtt gaaaagtctc
      361 aattgccctt tggtcttctg agactgtatc tttgatattt ttggagtaga caagtgtgtc
      421 gtgctccacc atgttgacga agattttctt cttgtcattg agtcgtaaga gactctgtat
      481 gaactgttcg ccagtcttta cggcgagttc tgttaggtcc tctatttgaa tctttgactc
      541 catgaagcta aactgaaggc gggaaacgac aatctgatcc aagctcaagc tgctctagca
      601 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
      661 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt
      721 ttcccagtca cgacgttgta aaacgacggc cagtgccaag cttgcatgcc tgcaggtccc
      781 cagattagcc ttttcaattt cagaaagaat gctaacccac agatggttag agaggcttac
      841 gcagcaggtc tcatcaagac gatctacccg agcaataatc tccaggaaat caaatacctt
      901 cccaagaagg ttaaagatgc agtcaaaaga ttcaggacta actgcatcaa gaacacagag
      961 aaagatatat ttctcaagat cagaagtact attccagtat ggacgattca aggcttgctt
     1021 cacaaaccaa ggcaagtaat agagattgga gtctctaaaa aggtagttcc cactgaatca
     1081 aaggccatgg agtcaaagat tcaaatagag gacctaacag aactcgccgt aaagactggc
     1141 gaacagttca tacagagtct cttacgactc aatgacaaga agaaaatctt cgtcaacatg
     1201 gtggagcacg acacacttgt ctactccaaa aatatcaaag atacagtctc agaagaccaa
     1261 agggcaattg agacttttca acaaagggta atatccggaa acctcctcgg attccattgc
     1321 ccagctatct gtcactttat tgtgaagata gtggaaaagg aaggtggctc ctacaaatgc
     1381 catcattgcg ataaaggaaa ggccatcgtt gaagatgcct ctgccgacag tggtcccaaa
     1441 gatggacccc cacccacgag gagcatcgtg gaaaaagaag acgttccaac cacgtcttca
     1501 aagcaagtgg attgatgtga tatctccact gacgtaaggg atgacgcaca atcccactat
     1561 ccttcgcaag acccttcctc tatataagga agttcatttc atttggagag aacacggggg
     1621 actcaaacat tactcattca caaaaccatc ttaaagcaac tacacaagtc ttgaaatttt
     1681 ctcatatttt ctatttacta tataaacttt taatcaaatc aagattaaag ttaattaaat
     1741 gacaaccttc aaaatcgagt cccggatcca cggcaacctc aacggggaga agttcgagtt
     1801 ggttggaggt ggagtaggtg aggagggtcg cctcgagatt gagatgaaga ctaaagataa
     1861 accactggca ttctctccct tcctgctgac cacttgcatg ggttacgggt tctaccactt
     1921 cgccagcttc ccaaagggga ttaagaacat ctatcttcat gctgcaacga acggaggtta
     1981 caccaacacc aggaaggaga tctatgaaga cggcggcatc ttggaggtca acttccgtta
     2041 cacttacgag ttcaacaaga tcatcggtga cgtcgagtgc attggacatg gattcccaag
     2101 tcagagtccg atcttcaagg acacgatcgt gaagagttgt cccacggtgg acctgatgtt
     2161 gccaatgtcc gggaacatca tcgccagctc ctacgcttac gccttccaac tgaaggacgg
     2221 ctctttctac acggcagaag tcaagaacaa catagacttc aagaatccaa tccacgagtc
     2281 cttctcgaag tcagggccca tgttcaccca cagacgtgtc gaggagactc tcaccaagga
     2341 gaaccttgcc atagtggagt accagcaggt tttcaacagc gccccaagag acatgtagtg
     2401 gtggtggtgg tatatgaaga tgaagatgaa atatttggtg tgtcaaataa aaagcttgtg
     2461 tgcttaagtt tgtgtttttt tcttggcttg ttgtgttatg aatttgtggc tttttctaat
     2521 attaaatgaa tgtaagatct cattataatg aataaacaaa tgtttctata atccattgtg
     2581 aatgttttgt tggatctctt ctgcagcata taactactgt atgtgctatg gtatggacta
     2641 tggaatatga ttaaagataa gatgggctca tagagtaaaa cgaggcgagg gacctataaa
     2701 cctcccttca tcatgctatt tcatgatcta ttttataaaa taaagatgta gaaaaaagta
     2761 agcgtaataa ccgcaaaaca aatgatttaa aacatggcac ataatgagga gattaagttc
     2821 ggtttacgtt tattttagta ctaattgtaa cgtgagacta cgtatcggga atcgcctaat
     2881 taaagcatta atgcgaacct gattagattc accgaccctc ctatcgtgtc gacctttctg
     2941 tttcttagaa ttttttggta gtctatgtac taataatgtc agcttcgtat ttatttcata
     3001 agcaatttgc atttgcaatt tgttttttac ttttattttt attgtattgt ggaatgtgga
     3061 ctcgtaccaa catgaagtta tataccacca aaaaaattac agttagtcaa aagattcacg
     3121 agtgagagct acttatgatt gtcttttacg tatatgtcta attgtctatt tgctcaataa
     3181 tctttgtact ttcttttgtc gttgataaaa tcacaaagtt ccaaaagtaa tcgaatgatt
     3241 tgcttttaag aaaagaagag ctcaataatt caacatatat ctgtacacaa ttaccgcctt
     3301 tgagtgagct ctagatgcca cctgacgtct aagaagaatt cgtaatcatg gtcatagctg
     3361 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     3421 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     3481 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     3541 gcggggagag gcggtttgcg tattggctag agcagcttgc caacatggtg gagcacgaca
     3601 ctctcgtcta ctccaagaat atcaaagata cagtctcaga agaccaaagg gctattgaga
     3661 cttttcaaca aagggtaata tcgggaaacc tcctcggatt ccattgccca gctatctgtc
     3721 acttcatcaa aaggacagta gaaaaggaag gtggcaccta caaatgccat cattgcgata
     3781 aaggaaaggc tatcgttcaa gatgcctctg ccgacagtgg tcccaaagat ggacccccac
     3841 ccacgaggag catcgtggaa aaagaagacg ttccaaccac gtcttcaaag caagtggatt
     3901 gatgtgaaca tggtggagca cgacactctc gtctactcca agaatatcaa agatacagtc
     3961 tcagaagacc aaagggctat tgagactttt caacaaaggg taatatcggg aaacctcctc
     4021 ggattccatt gcccagctat ctgtcacttc atcaaaagga cagtagaaaa ggaaggtggc
     4081 acctacaaat gccatcattg cgataaagga aaggctatcg ttcaagatgc ctctgccgac
     4141 agtggtccca aagatggacc cccacccacg aggagcatcg tggaaaaaga agacgttcca
     4201 accacgtctt caaagcaagt ggattgatgt gatatctcca ctgacgtaag ggatgacgca
     4261 caatcccact atccttcgca agacccttcc tctatataag gaagttcatt tcatttggag
     4321 aggacacgct gaaatcacca gtctctctct acaaatctat ctctctcgag ctttcgcaga
     4381 tcccgggggg caatgagata tggggattga acaagatgga ttgcacgcag gttctccggc
     4441 cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga
     4501 tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct
     4561 gtccggtgcc ctgaatgaac tccaggacga ggcagcgcgg ctatcgtggc tggccacgac
     4621 gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct
     4681 attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt
     4741 atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt
     4801 cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt
     4861 cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag
     4921 gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acacatggcg atgcctgctt
     4981 gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg
     5041 tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg
     5101 cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg
     5161 catcgccttc tatcgccttc ttgacgagtt cttctgaagt agatgccgac cggatctgtc
     5221 gatcgacaag ctcgagtttc tccataataa tgtgtgagta gttcccagat aagggaatta
     5281 gggttcctat agggtttcgc tcatgtgttg agcatataag aaacccttag tatgtatttg
     5341 tatttgtaaa atacttctat caataaaatt tctaattcct aaaaccaaaa tccagtacta
     5401 aaatccagat cccccgaatt aattcggcgt taattcagta cattaaaaac gtccgcaatg
     5461 tgttattaag ttgtctaagc gtcaatttgt ttacaccaca atatatcctg ccaccagcca
     5521 gccaacagct ccccgaccgg cagctcggca caaaatcacc actcgataca ggcagcccat
     5581 cagtccggga cggcgtcagc gggagagccg ttgtaaggcg gcagactttg ctcatgttac
     5641 cgatgctatt cggaagaacg gcaactaagc tgccgggttt gaaacacgga tgatctcgcg
     5701 gagggtagca tgttgattgt aacgatgaca gagcgttgct gcctgtgatc accgcggttt
     5761 caaaatcggc tccgtcgata ctatgttata cgccaacttt gaaaacaact ttgaaaaagc
     5821 tgttttctgg tatttaaggt tttagaatgc aaggaacagt gaattggagt tcgtcttgtt
     5881 ataattagct tcttggggta tctttaaata ctgtagaaaa gaggaaggaa ataataaatg
     5941 gctaaaatga gaatatcacc ggaattgaaa aaactgatcg aaaaataccg ctgcgtaaaa
     6001 gatacggaag gaatgtctcc tgctaaggta tataagctgg tgggagaaaa tgaaaaccta
     6061 tatttaaaaa tgacggacag ccggtataaa gggaccacct atgatgtgga acgggaaaag
     6121 gacatgatgc tatggctgga aggaaagctg cctgttccaa aggtcctgca ctttgaacgg
     6181 catgatggct ggagcaatct gctcatgagt gaggccgatg gcgtcctttg ctcggaagag
     6241 tatgaagatg aacaaagccc tgaaaagatt atcgagctgt atgcggagtg catcaggctc
     6301 tttcactcca tcgacatatc ggattgtccc tatacgaata gcttagacag ccgcttagcc
     6361 gaattggatt acttactgaa taacgatctg gccgatgtgg attgcgaaaa ctgggaagaa
     6421 gacactccat ttaaagatcc gcgcgagctg tatgattttt taaagacgga aaagcccgaa
     6481 gaggaacttg tcttttccca cggcgacctg ggagacagca acatctttgt gaaagatggc
     6541 aaagtaagtg gctttattga tcttgggaga agcggcaggg cggacaagtg gtatgacatt
     6601 gccttctgcg tccggtcgat cagggaggat atcggggaag aacagtatgt cgagctattt
     6661 tttgacttac tggggatcaa gcctgattgg gagaaaataa aatattatat tttactggat
     6721 gaattgtttt agtacctaga atgcatgacc aaaatccctt aacgtgagtt ttcgttccac
     6781 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     6841 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     6901 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     6961 actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     7021 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     7081 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     7141 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     7201 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     7261 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     7321 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     7381 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     7441 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     7501 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     7561 agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat
     7621 ctgtgcggta tttcacaccg catatggtgc actctcagta caatctgctc tgatgccgca
     7681 tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac
     7741 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca
     7801 gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga
     7861 aacgcgcgag gcagggtgcc ttgatgtggg cgccggcggt cgagtggcga cggcgcggct
     7921 tgtccgcgcc ctggtagatt gcctggccgt aggccagcca tttttgagcg gccagcggcc
     7981 gcgataggcc gacgcgaagc ggcggggcgt agggagcgca gcgaccgaag ggtaggcgct
     8041 ttttgcagct cttcggctgt gcgctggcca gacagttatg cacaggccag gcgggtttta
     8101 agagttttaa taagttttaa agagttttag gcggaaaaat cgcctttttt ctcttttata
     8161 tcagtcactt acatgtgtga ccggttccca atgtacggct ttgggttccc aatgtacggg
     8221 ttccggttcc caatgtacgg ctttgggttc ccaatgtacg tgctatccac aggaaagaga
     8281 ccttttcgac ctttttcccc tgctagggca atttgcccta gcatctgctc cgtacattag
     8341 gaaccggcgg atgcttcgcc ctcgatcagg ttgcggtagc gcatgactag gatcgggcca
     8401 gcctgccccg cctcctcctt caaatcgtac tccggcaggt catttgaccc gatcagcttg
     8461 cgcacggtga aacagaactt cttgaactct ccggcgctgc cactgcgttc gtagatcgtc
     8521 ttgaacaacc atctggcttc tgccttgcct gcggcgcggc gtgccaggcg gtagagaaaa
     8581 cggccgatgc cgggatcgat caaaaagtaa tcggggtgaa ccgtcagcac gtccgggttc
     8641 ttgccttctg tgatctcgcg gtacatccaa tcagctagct cgatctcgat gtactccggc
     8701 cgcccggttt cgctctttac gatcttgtag cggctaatca aggcttcacc ctcggatacc
     8761 gtcaccaggc ggccgttctt ggccttcttc gtacgctgca tggcaacgtg cgtggtgttt
     8821 aaccgaatgc aggtttctac caggtcgtct ttctgctttc cgccatcggc tcgccggcag
     8881 aacttgagta cgtccgcaac gtgtggacgg aacacgcggc cgggcttgtc tcccttccct
     8941 tcccggtatc ggttcatgga ttcggttaga tgggaaaccg ccatcagtac caggtcgtaa
     9001 tcccacacac tggccatgcc ggccggccct gcggaaacct ctacgtgccc gtctggaagc
     9061 tcgtagcgga tcacctcgcc agctcgtcgg tcacgcttcg acagacggaa aacggccacg
     9121 tccatgatgc tgcgactatc gcgggtgccc acgtcataga gcatcggaac gaaaaaatct
     9181 ggttgctcgt cgcccttggg cggcttccta atcgacggcg caccggctgc cggcggttgc
     9241 cgggattctt tgcggattcg atcagcggcc gcttgccacg attcaccggg gcgtgcttct
     9301 gcctcgatgc gttgccgctg ggcggcctgc gcggccttca acttctccac caggtcatca
     9361 cccagcgccg cgccgatttg taccgggccg gatggtttgc gaccgctcac gccgattcct
     9421 cgggcttggg ggttccagtg ccattgcagg gccggcaggc aacccagccg cttacgcctg
     9481 gccaaccgcc cgttcctcca cacatggggc attccacggc gtcggtgcct ggttgttctt
     9541 gattttccat gccgcctcct ttagccgcta aaattcatct actcatttat tcatttgctc
     9601 atttactctg gtagctgcgc gatgtattca gatagcagct cggtaatggt cttgccttgg
     9661 cgtaccgcgt acatcttcag cttggtgtga tcctccgccg gcaactgaaa gttgacccgc
     9721 ttcatggctg gcgtgtctgc caggctggcc aacgttgcag ccttgctgct gcgtgcgctc
     9781 ggacggccgg cacttagcgt gtttgtgctt ttgctcattt tctctttacc tcattaactc
     9841 aaatgagttt tgatttaatt tcagcggcca gcgcctggac ctcgcgggca gcgtcgccct
     9901 cgggttctga ttcaagaacg gttgtgccgg cggcggcagt gcctgggtag ctcacgcgct
     9961 gcgtgatacg ggactcaaga atgggcagct cgtacccggc cagcgcctcg gcaacctcac
    10021 cgccgatgcg cgtgcctttg atcgcccgcg acacgacaaa ggccgcttgt agccttccat
    10081 ccgtgacctc aatgcgctgc ttaaccagct ccaccaggtc ggcggtggcc catatgtcgt
    10141 aagggcttgg ctgcaccgga atcagcacga agtcggctgc cttgatcgcg gacacagcca
    10201 agtccgccgc ctggggcgct ccgtcgatca ctacgaagtc gcgccggccg atggccttca
    10261 cgtcgcggtc aatcgtcggg cggtcgatgc cgacaacggt tagcggttga tcttcccgca
    10321 cggccgccca atcgcgggca ctgccctggg gatcggaatc gactaacaga acatcggccc
    10381 cggcgagttg cagggcgcgg gctagatggg ttgcgatggt cgtcttgcct gacccgcctt
    10441 tctggttaag tacagcgata accttcatgc gttccccttg cgtatttgtt tatttactca
    10501 tcgcatcata tacgcagcga ccgcatgacg caagctgttt tactcaaata cacatcacct
    10561 ttttagacgg cggcgctcgg tttcttcagc ggccaagctg gccggccagg ccgccagctt
    10621 ggcatcagac aaaccggcca ggatttcatg cagccgcacg gttgagacgt gcgcgggcgg
    10681 ctcgaacacg tacccggccg cgatcatctc cgcctcgatc tcttcggtaa tgaaaaacgg
    10741 ttcgtcctgg ccgtcctggt gcggtttcat gcttgttcct cttggcgttc attctcggcg
    10801 gccgccaggg cgtcggcctc ggtcaatgcg tcctcacgga aggcaccgcg ccgcctggcc
    10861 tcggtgggcg tcacttcctc gctgcgctca agtgcgcggt acagggtcga gcgatgcacg
    10921 ccaagcagtg cagccgcctc tttcacggtg cggccttcct ggtcgatcag ctcgcgggcg
    10981 tgcgcgatct gtgccggggt gagggtaggg cgggggccaa acttcacgcc tcgggccttg
    11041 gcggcctcgc gcccgctccg ggtgcggtcg atgattaggg aacgctcgaa ctcggcaatg
    11101 ccggcgaaca cggtcaacac catgcggccg gccggcgtgg tggtgtcggc ccacggctct
    11161 gccaggctac gcaggcccgc gccggcctcc tggatgcgct cggcaatgtc cagtaggtcg
    11221 cgggtgctgc gggccaggcg gtctagcctg gtcactgtca caacgtcgcc agggcgtagg
    11281 tggtcaagca tcctggccag ctccgggcgg tcgcgcctgg tgccggtgat cttctcggaa
    11341 aacagcttgg tgcagccggc cgcgtgcagt tcggcccgtt ggttggtcaa gtcctggtcg
    11401 tcggtgctga cgcgggcata gcccagcagg ccagcggcgg cgctcttgtt catggcgtaa
    11461 tgtctccggt tctagtcgca agtattctac tttatgcgac taaaacacgc gacaagaaaa
    11521 cgccaggaaa agggcagggc ggcagcctgt cgcgtaactt aggacttgtg cgacatgtcg
    11581 ttttcagaag acggctgcac tgaacgtcag aagccgactg cactatagca gcggaggggt
    11641 tggatcaaag tactttgatc ccgaggggaa ccctgtggtt ggcatgcaca tacaaatgga
    11701 cgaacggata aaccttttca cgccctttta aatatccgat tattctaata aacgctcttt
    11761 tctcttaggt ttacccgcca atatatcctg tcaaacactg atagtttaat tcccgatcta
    11821 gtaacataga tgacaccgcg cgcgataatt tatcctagtt tgcgcgctat attttgtttt
    11881 ctatcgcgta ttaaatgtat aattgcggga ctctaatcat aaaaacccat ctcataaata
    11941 acgtcatgca ttacatgtta attattacat gcttaacgta attcaacaga aattatatga
    12001 taatcatcgc aagaccggca acaggattca atcttaagaa actttattgc caaatgtttg
    12061 aacgatcggg gaaattcgag ctggtcacca attcacacgt gatggtgatg gtgatggcta
    12121 gcgttcttgt agccgaaatc tggaatgttg gtccagcgct cgcgaaagac gtgcgcggcg
    12181 agcttcggct tgcggtcacg agtgaacacg cccttcttgt ttccttggac gcgcatcacg
    12241 ccctgagagg tcgcgaagtc cgcgaagttc cacgcttgct cacccacgaa gttctcaaac
    12301 tcatcgaaca cgacgtggtt cgcctggtag tactcgactt gatattcctc ggtgaacatc
    12361 actggatcaa tgtcgtgaaa gcccgcaacg gtgtctgcgc cgtactcagt gatcatgatc
    12421 ggctttcctg ggcaacgctt gttccacgcg tgaaattcct ggcggagatg gactttggcc
    12481 gcttcgagat caccgccatc gaagtaccat ccgttatagc gattgagcgc gatgacgtca
    12541 atcagttcgg cgactttgtc cgtctccggg gtagccatca caaacagcac gatcgtgacc
    12601 ggacgcttct gtgggtcgag ttccttggtc agctccacca acggcttgaa gtactcgtac
    12661 gcgccctctt cctcagtcgc cgcctcgttg gcgatgctcc acatcacgac gcttggatgg
    12721 ttcttgtcac gagacaccag ttcacggaga acgtcttgat ggtgctcaaa cgtccgaatc
    12781 ttctcccagg tactgacgcg ctcgctgcct tcgccgagtc ccgtggtggc catgaagttg
    12841 aggtgcacgc caactgccgg agtctcgtcg atcacgacca gaccctcgcg atccgcaaga
    12901 cgcatcaact cttcagagta cggatagtgt gcggtccgga agctgttggc gccgatccat
    12961 ttgaggatat tgaaatccat cacattgctc gcttcgttaa agccacggcc gttgatagga
    13021 gtgtcctcat gtttgccaaa gcccttgaag tagaacggtt tgttgttgat gaggaacttg
    13081 ccgtcgttga cttccacggt ccgcacgccg aacggctctt catagacatc gatggtcagt
    13141 ccgtcgttca ccagttccac tttgatctgg tagagatacg tgttcagtgg ttcccagagg
    13201 atgacattcg gaatctccac gttaccgctc aggccctcgg tgcttgcgac cactttgcct
    13261 tcctcatcca cgaccgacac tttcacggtc tcggctttgc cttgaaagtc caccgtatag
    13321 gtcacagtcc cggttgggcc attgaagtcg gtcacaaccg agatgtcctc gacgtacgta
    13381 aacggggtcg tgtagatttt caccggacgg tgcaggcctg catagttgaa gaagtcgaag
    13441 ttcggcttgt tacgaatgac ttttccgagg ccctcttcgt ggcgctcgct gtacagcccc
    13501 accgggaggg tgctatcgtc gaggatgttg tccacggcga cggtgacgcg attcatgcca
    13561 tcacgcagcg agttgttgat ttccgcttcg aatggcagga atccgccctt gtgctccacg
    13621 accagctcac cattgacata gacaattgct ttgtgagttg cagagccgaa gcggagcacg
    13681 atacgctgat ccttcagata ggccggcacc gtgaactcac gttcgtacca gacatatccg
    13741 atatggttgc ggatttcctt ggtcacgcca atgtcattgt aactgcttgg gacggccata
    13801 ctaatagtgt cggtcagctt gctttcgtac cacttctctt ccagtccttt cccgtagtcc
    13861 agcttgaagt tccagacgcc attgaggtcg aagacgccac gggtctcggt gttgatcggg
    13921 tacagactag ttcgtcggtt ctgtaactat catcatcatc atagacacac gaaataaagt
    13981 aatcagatta tcagttaaag ctatgtaata tttacaccat aaccaatcaa ttaaaaaata
    14041 gatcagttta aagaaagatc aaagctcaaa aaaataaaaa gagaaaaggg tcctaaccaa
    14101 gaaaatgaag gagaaaaact agaaatttac cctcagatct accatg
//
